Zfyve16 (NM_001191958) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1564784
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217652 representing NM_001191958 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGTTACTTTAAAGCAGCTGTCAGTGACTTGGACAAACTTCTTGATGATTTTGAGCAGAACCCAG AAAAACAAGATTACTTCCAAGATGTGCAGGATGTTTATGCTTTTAACCAGTGTTCAGTTTCTTCAGAGTT GGCCTCTCCACAGCTAGCTTTACTGTCAAAGGACCAAAGATGCATTAGTAGTCATGCCTCATCAGAAACA TGCTGTGAAGCAAATGAGACTTTTCTCCAAGGAAAGATACCTGAGGGGTTAACCTCTAAACAGAATGAAA AAAACGTAGCGGGACTTGATCTTCTTTCTTCTGTGGATGCTAGTACTTCAGATGAGATCCAGCCTTCATG TATGAGACGATGTAGTAAGCCTGTCTGTGACCTGATAAGTGACATGGGTAACTTAGTACACGCCACCAGT AGTGAGGAAGACATTAAACAGTTATTGCCAGATGACTCGAAGTCTCCTGCAGATACTTTGATCACACTGG ACTCATCTTCAGTTTCAGAGGTTTTGATCGGATTGGATTTGTCTTCAGTTTCAGATACTCTCGCTGTTTC TTCAGTAGACGGTGGTAGTCAGGCTGTCAGGAAAGAGCAGGATACCAACGCTGGTGTGCAAAGCAGAGAT AACAGTAGGATCCAAGAATCGGGTGCAGAAGTGGACATGGCACTTTCTGATTCTTGTAAATATAGTAGAA CAGAAAATTTAAAGGATAAAATGATTTCTAATGAGTTAGAAACAGTTGATTTTGGCATGCCATCTGTTTT GACTGAACAGAGTTCAGAAATGTCCAATACCAAAGATAGCCCACAATACCAGAGGCTGCCATGTGAGCTG TTAAAAGATGACGGTGATTTGGCAGAAGAGAAAGTAGGCATGGCAGGCAATAGTACAAAGTGTTTAGAAG AAGGAGGTAACATAATTGCTATGCCTTGCAAGCTTCCAAAAAATGAAGAGATATCCCCAAATGACCCAAC TTCAAAAGATGAAAACTTCAAGTTACCTGACTTTTCTGTCCAGGAAAGTAGAACTGCTGTGTTCATAAAG CAGACTGTGGCAGAGGACTCGGTAAAGTCAGACCTTAAAGATAATAATGATGTCGTCCAAGTTTCTTCTT CATCTCTACATGTTTCGAGTGATGGTGTGTCCCCTTCACTGTCCTGTCTTTCATTGTCTGGGTCTTTGTG TGGATCCTTAGTTGACTGTAATGCACATGGTGATTTTTTACCCTCTGATGAGTCTAAAGGTCAGAACAAT GACGCAGTAACTGCACATGAGGAGATACAGAAGAGTGCCGCTTTAGATGGGGAGACAGATCTCCTGAAGA AGGAAAAGCAGGAAAGCATGTTTCTTCAGCCAGTAAATGATAAGGAAGGGGATGGAGAGGTTGAACTTGA GGAGATGGTAATCAGCGGTGAGCCTTTGGAGTACCCTGAGGGTACCAACCCTGCCGCAGCTGGCTCTCAA ATGGCACCCCCTACTGTGAGTGCCTCAGAGGCTCCTGGTCAGTGCGAAGCTCTCACTTTTTCCAGTGGCG ATATGGATGGGCAAGAGTTAGATTACTTTAATATTGATGAAAGCATGAGAAGTGGCATACTGGTTAGCGA TGCTGAACTTGATGCTTTTCTGAAGGAACAGAGTCTTCAGAACTCTAACACCATGTCTTTTGGAGAAAAT GCAAGTGATTCACAATTGCAGATGAATCAGATAGATATGAAAGGATTATGTGATGAAAATGCTGGTGATA GATACTTCAATGCTGAAGCAGGAGCTGCTGGCGAAAGTGGAGGCGTTGTGATTTGTGAAACAACTGATAA AGAAAACACAGTACAAAATGGTGGTCTTTCTGTAGGAGAAAAAAGCGCAATTCCAACGGAAAGAGGGTTA TCTGCTAGTCAGCCTGACATACGGAGTGAAGTGCCAGTCCCCAGTATTAACACCCAGGCTGTCGGAGGAG CTAGACCTAAGCAGCTGCCGAGTCGTCCACCAGGAACAAGCAGTTCACAGGGACCGAGTAAGCCACATGT TGTAGGTGTGCCAGCAGGCGAGCCAAGCACAGCAAATGCTGCTGCTGCACCCACTCGCCCTGCAGATCAT ACACCTGATTCTCAGGTTAGCTTCAACTCCAATTATATCGACATAGAAAGTAATTTCGAAGGTGGATCCA GTTTTGTAACAGCAAACAAGGATTCACTGCCTGAGAATATACGGAAAGAAGGCTTGGTTTTGGGCCAGAA ACAACCTACGTGGGTTCCTGATTCAGAAGCTCCAAACTGTATGAACTGCAAAGTCAAATTCACTTTTACA AAACGGCGACACCACTGCCGAGCTTGTGGGAAAGTATTTTGTGGTGTCTGTTGTAACAGGAAATGTAAGC TTCAGTATCTAGAAAAGGAAGCAAGAGTGTGTGTGATCTGCTATGAGACTATTAATAAAGCTCAGGCATT TGAAAGGATGATGAGTCCAGGTGGTGCTTGTCTTAAGTCTAATCATTCTAATGAGTGTGTCACTGCCCAG CCACTTCAGGAGACCCAAACGTCCAGCATACCTTCACCTACAGCTTTGCCCATCTCAGCACTGAAACAAC CAAATGTCGAAGGACCATGTTCCCGAGAACAGAAGAGAGTATGGTTTGCAGATGGCATACTGCCGAATGG TGAAGTTGCAGATACTACGAAATTATCCTCTGGAAGTAAAAGGTGTTCTGATGACTTTAGTCCTGTCTTA CCTGATGCGCCCACAGTGATAAATAAAGTGGACCATACCCCCTCTCCTATGGTGGAGAAAGCAAACAACA GTGTAGGAGACGTTCTAAGAAGTGAGATAACTCAGAGTCCGATTTGTCGTGCTGCACCTCTGGAAATACT GCCTGGGAGCACAGGCGCTGAAGGCTTGCCCACAGCTGGCCCTTTCACACTAGAAGATGATGTTTTTGTG GACAGTGAGGAGCCATCTACTCCTACTGTTGTCCCTGCTAATACTGGTTTGCCTGTTGCTAGTGTTTCAG ACTATAGGTTATTGTGTGGTGTTGCCAAATGTGTCTGCAATAACATTAGTCTTCTACCAGATGACGATGT TGGTTTGCCTCCCCTTCTGGCCACATCTGGAGAAGATGGATCAGTGCCTGTAGTACAAGAACATCCATCT CATGAGCAGATCATTTTGCTTCTTGAAAGTGAAGGATTTCCTCCTGCCACATTTGTTCTAAATGCAAACC TACTTGTGAATGTCAAGTTGGTATTTTATTCCTCAGATAAGTATTGGTACTTCTCAACCAATGGATTGCA TGGTTTGGGACAGGCAGAAATTATTATCCTGTTGCTATGCTTGCCAAATGAGGATACAGTTCCTAAGGAC ATCTTTAGACTATTCATCACCATATATAAGGATGCTTCGAAAGGGAAGTACGTAGAAAACTTGGACAATC TGACCTTTACTGAGAGTTTTCTCAGTAGCAAGGATCACGGAGGGTTCCTATTCATCACACCTACCTTTCA GAAACTTGACGATCTCCCAGTACCAAGGAGCCCTTTTCTCTGTGGCATTCTCATCCACAAGCTGGAGATT CCCTGGGCAAAGGCTTTCCCTATGCGTCTCATGTTGAGATTAGGCGCAGAATACAAAGCATATCCTGCTC CTCTAACAAGTGTCAGAGGCAGGAGACCGCTTTTCGGAGAAATAGGACATACTATTATGAACTTACTTGT TGATCTTCGAAATTACCAGTATACATTGCATAACATCGATCAGCTGTTAATCCATATGGAAATGGGGAAA AGCTGTATAAAGATACCTCGGAAAAAGTACAGTGATGTATTGAAGGTAATAAATTCTTCTAACGAGCACG TCATCAGTATTGGGGCTAGTTTTAGTACAGAAGCAGATTCTCATCTGGTCTGTGTACAGAGTGATGGAGT TTATCAGACTCAGGCCAACAGTGCCACTGGTCAGCCAAGGAAAGTGACAGGTGCAAGTTTTGTGGTGTTC AATGGTGCTCTGAAAACATCTTCAGGATTTCTTGCCAAATCCAGCATAGTCGAAGATGGCTTAATGGTTC AAATAACCCCAGAGACCATGGATGGCTTACGGCTGGCTCTACGAGAGCAAAAGGACTTTAAAATTCAGTG TGGAAAAGTTGATGCAGTAGACCTGAGAGAATATGTGGACATCTGTTGGGTGGATTCGGAAGAAAGGAAA AACAAAGGTGTTATCAGTTCAGTGGATGGGATGTCATTAGAAGGATTTCCAAGTGAAAAAATAAAACTGG AAACAGATTTTGAAACTGAAGAGAAGACTGTGAAATGCACTGAGGTGTTCTGCTTCCTAAAGGACCAGGA TATCTCTCTCTCCTCGAGTAGTTACCAGTTTGCAAAAGAGATCGCCACGGCTTGCAGTGCGGCCCTGTGC CCTCACCTGAGGACTCTAAAGAGTAACAGAATGAACAGAATTGGACTCAGAGTTTCTGTTGACACTGATA TGGTTGAATTTCAGGCAGGCTGTGAAGGGCAGCTTCTGCCTCAGCATTACCTAAACGATCTTGATAGCGC TCTGATACCTGTGATCCACGGCGGGACCTCCAACTCGACCGTGCCACTCGAGATAGAGTTAGCATTCTTC ATTTTAGAAAACCTTCTGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217652 representing NM_001191958 Red=Cloning site Green=Tags(s) MDSYFKAAVSDLDKLLDDFEQNPEKQDYFQDVQDVYAFNQCSVSSELASPQLALLSKDQRCISSHASSET CCEANETFLQGKIPEGLTSKQNEKNVAGLDLLSSVDASTSDEIQPSCMRRCSKPVCDLISDMGNLVHATS SEEDIKQLLPDDSKSPADTLITLDSSSVSEVLIGLDLSSVSDTLAVSSVDGGSQAVRKEQDTNAGVQSRD NSRIQESGAEVDMALSDSCKYSRTENLKDKMISNELETVDFGMPSVLTEQSSEMSNTKDSPQYQRLPCEL LKDDGDLAEEKVGMAGNSTKCLEEGGNIIAMPCKLPKNEEISPNDPTSKDENFKLPDFSVQESRTAVFIK QTVAEDSVKSDLKDNNDVVQVSSSSLHVSSDGVSPSLSCLSLSGSLCGSLVDCNAHGDFLPSDESKGQNN DAVTAHEEIQKSAALDGETDLLKKEKQESMFLQPVNDKEGDGEVELEEMVISGEPLEYPEGTNPAAAGSQ MAPPTVSASEAPGQCEALTFSSGDMDGQELDYFNIDESMRSGILVSDAELDAFLKEQSLQNSNTMSFGEN ASDSQLQMNQIDMKGLCDENAGDRYFNAEAGAAGESGGVVICETTDKENTVQNGGLSVGEKSAIPTERGL SASQPDIRSEVPVPSINTQAVGGARPKQLPSRPPGTSSSQGPSKPHVVGVPAGEPSTANAAAAPTRPADH TPDSQVSFNSNYIDIESNFEGGSSFVTANKDSLPENIRKEGLVLGQKQPTWVPDSEAPNCMNCKVKFTFT KRRHHCRACGKVFCGVCCNRKCKLQYLEKEARVCVICYETINKAQAFERMMSPGGACLKSNHSNECVTAQ PLQETQTSSIPSPTALPISALKQPNVEGPCSREQKRVWFADGILPNGEVADTTKLSSGSKRCSDDFSPVL PDAPTVINKVDHTPSPMVEKANNSVGDVLRSEITQSPICRAAPLEILPGSTGAEGLPTAGPFTLEDDVFV DSEEPSTPTVVPANTGLPVASVSDYRLLCGVAKCVCNNISLLPDDDVGLPPLLATSGEDGSVPVVQEHPS HEQIILLLESEGFPPATFVLNANLLVNVKLVFYSSDKYWYFSTNGLHGLGQAEIIILLLCLPNEDTVPKD IFRLFITIYKDASKGKYVENLDNLTFTESFLSSKDHGGFLFITPTFQKLDDLPVPRSPFLCGILIHKLEI PWAKAFPMRLMLRLGAEYKAYPAPLTSVRGRRPLFGEIGHTIMNLLVDLRNYQYTLHNIDQLLIHMEMGK SCIKIPRKKYSDVLKVINSSNEHVISIGASFSTEADSHLVCVQSDGVYQTQANSATGQPRKVTGASFVVF NGALKTSSGFLAKSSIVEDGLMVQITPETMDGLRLALREQKDFKIQCGKVDAVDLREYVDICWVDSEERK NKGVISSVDGMSLEGFPSEKIKLETDFETEEKTVKCTEVFCFLKDQDISLSSSSYQFAKEIATACSAALC PHLRTLKSNRMNRIGLRVSVDTDMVEFQAGCEGQLLPQHYLNDLDSALIPVIHGGTSNSTVPLEIELAFF ILENLLE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191958
ORF Size	4641 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191958.1, NP_001178887.1
RefSeq Size	5372 bp
RefSeq ORF	4644 bp
Locus ID	499508
MW	167.5 kDa

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