Gli3 (NM_080405) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217660 representing NM_080405 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCAGTCCCACAGCTCGACGACAACTGAGAGGAAGAAAGCTGAAAGTTCCATTGCGAAGTGTC CCACAAGAACAGATGTCAGTGAGAAGGCCGTGGCCTCCAGTACCACTTCAAATGAGGATGAAAGTGCTGG ACAGATCTATCACCGAGAGAGAAGAAACGCAATCACTATGCAGCCTCAGAGTGTGCAGGGTCTCAGCAAA ATCAGTGAGGAGCCCTCAACATCTAGTGATGAGAGGGCCTCGTTGATCAAGAAAGAGATCCATGGATCTC TACCACATCTGGCTGAGCCCTCTCTCCCTTATCGTGGGACTGTGTTTGCCATGGATCCCCGGAATGGTTA CATGGAGCCTCACTACCACCCTCCTCATCTTTTTCCTGCCTTCCATCCTCCTGTACCGATTGATGCCAGA CATCACGAGGGCCGCTACCATTATGATCCATCTCCTATTCCTCCATTACACGTGCCTTCTGCCTTATCTA GTAGTCCAACGTATCCAGACTTGCCTTTCATTAGGATCTCCCCACACCGTAACCCCACTGCAGCTTCAGA GTCCCCCTTCAGCCCCCCACACCCCTACATCAACCCATATATGGACTACATCCGCTCCTTGCACAGCAGC CCATCCCTCTCCATGATCTCCGCTGCCCGAGGGCTGAGCCCCACAGATGCCCCCCATGCTGGAGTCAGCC CTGCGGAATACTATCACCAGATGGCTCTGCTGACAGGCCAGCGCAGCCCCTATGCAGACATCCTTCCCTC AGCCGCCACTGCTGGTGCAGGGGCCATCCATATGGAGTACCTTCATGCCATGGACAGCACCAGATTTCCC AGCCCTAGACTGTCAGCTAGGCCCAGCCGAAAGCGTACCCTGTCGATCTCTCCACTGTCAGATCATAGCT TCGACCTTCAGACCATGATAAGAACATCTCCTAACTCCTTGGTCACAATCCTCAATAATTCCCGTAGCAG TTCTTCAGCAAGTGGTTCCTATGGGCACTTATCTGCAAGTGCAATCAGCCCTGCTTTGAGCTTCACCTAC CCCTCCGCCCCCGTGTCTCTCCATATGCATCAACAGATCCTAAGCCGACAGCAGAGCTTAGGTTCAGCAT TTGGACATAGCCCTCCCCTCATCCACCCTGCTCCAACATTTCCAACGCAGAGACCCATCCCTGGGATTCC GACGGTTCTGAACCCTGTCCAGGTCAGCTCTGGCCCTTCTGAGTCTTCACAGAGCAAACCCACAAGTGAG TCTGCAGTGAGTAGTACCGGTGACCCTATGCATAATAAGCGGTCCAAGATCAAGCCCGATGAAGACCTCC CCAGCCCAGGATCACGGGGGCAGCAGGAACAGCCGGAGGGAACAACCCTAGTCAAGGAGGAAGGGGACAA AGATGAAAGCAAGCAGGAGCCTGAAGTCATCTATGAGACAAACTGCCACTGGGAAGGCTGCACAAGAGAG TTCGACACCCAGGATCAGCTTGTGCATCATATCAATAATGACCACATTCATGGAGAAAAGAAGGAGTTTG TGTGCCGCTGGCTTGATTGTTCAAGAGAGCAGAAACCGTTCAAAGCCCAGTACATGTTGGTAGTACATAT GAGAAGACACACCGGAGAGAAGCCTCACAAATGTACATTTGAAGGTTGTACAAAAGCCTACTCAAGACTC GAAAACCTGAAAACCCACTTGAGATCTCACACTGGAGAGAAGCCATATGTCTGTGAGCATGAGGGCTGCA ACAAGGCTTTCTCTAACGCTTCAGATCGGGCCAAGCACCAAAACAGAACACATTCCAATGAGAAACCATA TGTATGCAAAATCCCTGGCTGCACCAAGCGTTACACAGACCCCAGCTCTCTCCGGAAACATGTGAAGACT GTGCATGGCCCTGAGGCTCATGTTACCAAGAAGCAGCGTGGGGACATGCACCCTCGGCCTCCACCACCTA GAGATTCTGGCAGCCATTCACAGTCCAGGTCCCCCGGCCGGCCGACTCAGGGAGCATTCGGTGAGCAGAA GGAGCTGAGCAACACTACCTCAAAGCGGGAAGAATGCCTCCAGGTGAAGACTGTCAAGGCTGAAAAGCCC ATGACATCTCAGCCAAGCCCTGGTGGTCAGTCTTCATGCAGCAGCCAACAGTCCCCCATCAGCAACTATT CCAACAGTGGGCTCGAGCTTCCTCTGACTGATGGAGGTAGTATAGCAGACCTCAGTGCCATCGATGAGAC CCCAATCATGGACTCGACCATTTCCACGGCAACCACAGCCCTTGCTTTGCAAGGCAGGAGAAACCCAGCA GGGACCAAATGGATGGAGCATATCAAACTTGAAAGGCTAAAGCAAGTGAATGGAATGTTTCCAAGACTGA ACCCTATTCTACCCTCCAAAGCCCCTGCGGTATCTCCTCTCATAGGAAATGGCACACAGTCAAATAACAA CTACAGTTCAGGCGGGCCCGGGACCCTTCTCCCGAGCAGAAGTGATCTGTCGGGTGTAGACTTCACTGTG CTGAACACGCTCAACAGGAGGGACAGCAACACCAGCACCATCAGCTCTGCCTACCTGAGCAGCCGCAGAT CCTCGGGCATCTCCCCCTGCTTTTCCAGCCGCAGGTCCAGTGAGGCATCGCAGGCTGAAGGGCGACCCCA GAATGTGAGTGTGGCCGACTCCTACGATCCCATCTCCACAGATGCTTCACGGAGGTCCAGCGAGGCCAGC CAGGGTGATGGGCTGCCCAGTCTGCTCAGCCTCACACCCGTGCAGCAGTACCGCCTGAAGGCCAAGTATG CTGCTGCCACGGGTGGCCCGCCACCCACACCTCTGCCCCACATGGAAAAGCTGAGCCTGAAGACCAGGAT GGCCCTGCTCGGGGAGGGGAGGGACTCAGGGGTGACTCTGCCTCCAGTCCATCCTCCTCGGAGATGCAGT GATGGAGGAGGCCATACATACAGCAGGCGTCACCTACTGCCTCATGATGCGCTAGCAAACAGTGCGAGGA GAGCCAGCGACCCTGTGAGGACCGTCTCCGAGAACATGTCACTTCCCAGGGTTCAACGCTTCAGCAGCCT GAATAGCTTTAATCCTCCAAATTTGCCTCCATCCGTGGAAAAGCGTAGTTTAGTGCTGCAAAATTATACC CGGCAGGAGAGCACCCATCCCCGGTACTTCCAAGCATCCCCTTGCCCTCCCAGCATCACAGAGAATGTTG CCCTGGAGGCCCTGACCATGGATGCTGATGCTAGCTTGAATGATGAAGATTTCCTGCCAGATGATGTGGT ACAGTATTTGAATTCCCAGAACCAAACAGGGTATGGGCAGCAATTACAGAGTTCCATCTCTGAAGATGGC AAAGTAGCCCGTGAGCCAGAGGACTTGGACTTACCGGGGCTGCCAGACAGTCATGTTGGCCAGCAATACC CAGCTCTGGAGCAACCCTGCTCCGAGGGCAGTAAAACTGATTTGCCCATCCAGTGGAATGAGGTCAGCTC TGGAAGTTCTGATCTGTCATCCTCCAAGCTGAAGTGTGGTCAGCGCCCTACCGTACAGCAGGCTCGAGGC TTTGGGTTATACAGCAATATGGTGGTACATCCACAGAACCTGTGGAAGGTTGGCACTGGCCCAGCTGGGG GATCTCAGACCCTTGGGGAGAATGGCAGTTCCTACAGTGACCCAGAGCACTTTGCAGTCCACAGTGGGGA TGGACTTGGCCCCAGTGGAGATACTTTCCATGAACAGGCCTATAAGACCCAGCAGTATGGGAGCCAGCTC AGCAGGCAGCCACTGACTTCCAGTGTTCTAGACAGTGCCTGTGGTGCTGGGATTCAAGGGTCCAAGCTGA AAGGCAACAGTTTGCAAGAGAATGGGGGTTTGCTAGATTTTGGCCTGTCCATGGCACCAAATGAGTTAGC TGACAACATAGTGAATGGCATACAAACCCAAGAACAAATGGGGCAGGGGTACATTGCTCCTCAGCTACTC AGTGGCAGCATGCAACACCAGGGGCCCAGTCGCCCTGGTCAACAGGTACTAGGGCAGGTTGGTGCTACCT CACATATCAACATCTATCAAGGGACAGAGAGCTGCCTACCAGGGACTCAGGACAAGATCAGCCAGCCATC AAGTATGGCAGTTATCAGGGGCTACCAGCCCTGTGCCAGCTATGGGGGCAGCAGGCGTCAGGCAATGCCA AGGGGCAGCCTCACTCTGCAACAAGGACAGCTCAGTGACGTGAGTCAGACCAGCAGGGTGAACAGCATCA AAATGGAGGCACACGGGCAGTCCCATCAGCTCTGCTCTAGCATGCAGAATTATTCCGGTCAGTTTTATGA CCAAACCATGGGCTTCAGTCAGCAAGACAGGAAAGCTGGCTCATTCTCCCTCTCAGAGGCCAACTGCCTG CTCCAGGAGAATGGCTCTGAAAATTCTGAGTTACTTTCCCCAGGTGTTAACCAGGTGACCAGCACAGTTG ACAGCTTTGAGAGTCATGACCTAGAAGGCGTGCAGATTGATTTTGATGCCATCATAGATGATGGGGACCA TACCAGCCTAATGTCAGGGGCCTTGAGCCCCAGTATCATTCAGAACCTTTCCCACAGCTCCTCCCGTCTC ACCACACCTCGGGCATCCCTCCCATTCCCTTCTCTGTCCATGAGCACCACCAACATGGCTATTGGGGATA TGAGTTCTTTGCTGACCTCCCTTGCAGAAGAAAGCAAGTTCCTTGCAGTTATGCAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_080405
Insert Size	4749 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_080405.2, NP_536330.2
RefSeq Size	5027 bp
RefSeq ORF	4749 bp
Locus ID	140588
Gene Summary	transcription factor; member of the C2H2-type zinc finger protein subclass of the Gli family of transcription factors; mediates sonic hedgehog (Shh) signalling; may play a role in embryogenesis [RGD, Feb 2006]

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