Vcan (NM_001170560) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217671 representing NM_001170560 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAACAG AATATATCAGAGGCAACCACCATCGAAACGAATACCCTCGCAGAAACTGCATCACCCAGTTTATCCAAAG AACCACACATGGTACCCGAGAGAGCTACACCAGTCATTCCATTAGTCACCGAATTACCTATTTTTACCAC ACACTTCCCTCCTGCAGGAAATATAGTCAATTCTGAACAAAAATCTGTAATCTACTCTCAGGCTATCACA GACAGAGTAGCCACTGAATCACCCACAGCTGCTGGGAGTCCCATTAACTCTTGGGATGCGGATGACTACT TGCCTTCTGGGTCAGGACCACTTGGAAAGCCAGACATATCTGAAATTAAGGAGGAAGGACTTCAAAGTAC AACTGTCACCTCCCAACATGCTACTGCCTCACACCATGGAATTACAGAGGATACGCAAACACATGAATCA GTTACACAGATTGAACAAATAGAAGTGGGCCCCTTGGTAACATCCATGGAAATCACAAATCACATTTCTT TCAAGGAATTTCTAGAAACCAAAACACCATTGGAATCCACAGAAGTGACCCTGGAACAGCATCAGACCGA CATGCCAACAGTTATCACCAGTCCTGAATTGGCAACCACAAGTCACTATGGAGTCACCTTGAGAGAAGAT GATAGAGAAGATATAGCACTGACAGTAAGATCTGGTCAGAGTACCCGGGTCTTTAGCCAAATACCAGAAG TCATCACGGTATCAAAGACATCAGAAGACACTACCTACTCACAGCTAGGGGACTTAGAATTCGTTTCAAC AGCAACAATAACCATGCTTGGCACAGATGGATCACTGACAGATGAAGGGAAAGAACCCCAAACTGATGAT AAGACGGCCGAAGACGAGTTTGGTCAATCACAACCTACAATCCCCTTCCCATCACAGCATCTTACAGAAG TAGAATCACTTCCATATTCTGGTGACACAATATCAGTAGAGAGAATATCCACAGTTAGTTATCCATCATT ACAAACAGATGTGACACAAGGAAGAGAGAGAACAGAAGTACCAAGGCCAGGGTTAAAAAAAGATCCTTAT GCAGTTGATGAGATACAAGAAAAGATCACCAAAGATCCATTTATAGGTACAATAGAAGAAGGCTTCTCTG GAATGTCTCTCTCTACATCTTCCTCAGAGACTTCAGCGGAAAGGACGGAATCTGTTTCCCCAGCATTGAC AATTGAGAAGTTAACTGTGAAGCCAGCCGTAGCAAGCGATGTGGACGAAATGACAATCCTGACTAGATTA GAAACAGATGTGCCAACGTCTGACGAAGATGTTACAAGTGCTCATTTGACCCATAGTACTTTAAATGTGG AAGTGGTCACAGTATTAAAATGGCCAGGGAATGAAGACAATTCAACTTCAAAGCCTTTACCCCCAACAGA ACGTGCAGGCTTTACAAAATCGCCTCCTGTCTCACTTAGCACTGTAGGAATAGTTGGAAAAGATAAAGAA ACCCCAAGTTTCACAGATGGGGGAGACGAATACACTCTTTCCCTAGATGGTACTCCCAAGCCATTAGAAA AGTTTTCTGAGGAAGATTTAACATCTGGAGAATTTGCAGTTACAATTCCTACATCTATATCAATTGACAG TGCAGAGAAGTCAACTTTGGGAGAGCCTACAACTGGAGACAGGGTTCTGTCCACCACAAGCACAAAAGAT CTGGTCATCAATGCAACTGTGGAAGGAAGTGCTTTGGATGAAGATATGGATGCCTCCAAGCCACTGTTTA CTGCCACCCCATTTGTCCACACCTCCGATGTGGAAGAATCGGCATTTGTTAATTATAGTAGCACCCAGCA GCCCACTACTTATGTAGACATATCCCACACTAGTCCTCTTTCTATAATTCCCAAGACAGAATGGAGTGTA TCGGAAACTTCTGTTCCACTGGAAGATGAGGTTCTAGGCAAATCTGATCAAGATACCCTTGAACAGACAC ATCTAGAAGCAACCATGTCTCCTGAAGCTTTGAGTACAATAGAAGTCACACAGGGAGAAACCCAGGAAGA ACCACAAACACCAGGAATCCCATTTCCTGCTCTCAGTTCTACTGCAGTTATGACCAAGGAGACAACAGCA TTTGAAGAAGAGGGGGAGGGGTCAACATATACCCTCTCTGAAGACAGATTGATGACAGATTCTGAAATAG TCCCAAGTTTAGAAACAACTCCAGTTGGTACATCTTATCCAGGTGGTGCAATGACACAGCAGGGAGTAGA AATGGATACAATGGTAACACAGATGTCAAGCATTAGGCCAACAGTAGTTTTAAGTACTGAGCCAGAAGTA AGTTATGAAGCAGAAGGTAGTAGTCCAATGGAATTTGCATCGACTTTGAAGCCCTTTGGGACTCAAGTCA CACAGCTTGTGGAAGAAACGACTGAGGAAGGGAAAAAGACACCCTTGGATTATACAGATTTAGGTTCAGG GTTATTTGAACAACCGAGAGTTACTGAGCTCCCAGATTTTTCAATGACTCCAAGTGATATCAGTGTCTTC ACTGCCATCGACAGCCTTCACAGAACCACACCCTTGAGGCCACCTTCGCCATTCACTGAGGAACCACACA TCTTTGAGAAGGAGCCCAGTGAGAAAACAACAGGTGACATAATACTTCCTAGAGAATCTGTAACACAGCA CCCTCTGACAACTCTCATGGATATCATAGCCAAGAAAACAGAATCTGATATTGATCACGAGTATCACATG ACCTCAAAGCCTCCTGTAATGCAGCCAACAAGACCATCAGTTGTGGAAAGAAAAACAACTTCCAAACCTC AAGAGTTGTCTACTTCATCACCCCCGGCAGGGACCAAATTCCACCCTGACATAAATGTTTATATTATTGA GGTCAGAGAAAACAAGACAGGACCTGATCTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTAT CCTACTGAGACTTCCTATGTGTGCACCTGTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTG ATGAATGTCACTCTAACCCTTGTCGGAATGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCT CTGCCTTCCGAGTTATGTCGGTGCACTCTGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAA TTCCAAGGGCAATGCTACAAGTACTTTGCTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCC TGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGA TTACCAGTGGATTGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTG CAATATGAGAACTGGAGACCCAACCAGCCAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCA TTTGGCATGAGAATGGCCAGTGGAATGACGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGG AACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTAT GAAATCAACTCCTTGATTAGATACCACTGCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGT GCCTAGGAAATGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTA TTCTAAGAAATACTTAAAAAATTCCTCATCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCAT CGCTGGAGCCGGAGGTGGCAGGAAACGAGGCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170560
Insert Size	4866 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170560.1, NP_001164031.1
RefSeq Size	7073 bp
RefSeq ORF	4866 bp
Locus ID	114122
UniProt ID	Q9ERB4
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006] Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3, also referred to as V2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data from several strains because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.

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