Vcan (NM_001170560) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cspg2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217671 representing NM_001170560 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGATAAATATGAACGGCATCCTATGGATGTGCTCAACCTTACTGTTAACGCATGCACTGCATAAAG CCAAAATGGAAGAAAACCCACCTGTTAAAGGCTCTCTGTCTGGAAAAGTGATCCTACCTTGTCATTTTTC AACCTTGCCCACCTTACCACCCGATTACAACACGAGTGAATTTCTCAGAATCAAATGGTCTAAAATAGAA GTGGACAAAAATGGAAAAGACATAAAGGAGACTACTGTCCTGGTGGCCCAAGACGGGAACATCAAGATTG GTCAGGACTACAAGGGGCGGGTATCAGTGCCTACGCATCCCGATGACGTAGGCGATGCCTCTCTCACCAT GGTCAAACTCCGTGCTAGTGACGCAGGTGTCTACCGCTGTGATGTCATGTATGGCATTGAAGACACTCAG AACACGATGTCGCTGGCCGTGGACGGTGTCGTGTTTCACTACAGGGCAGCGACCAGCAGATACACTCTGA ACTTCGAGTCTGCTCAACAGGCTTGTTTGGACATCGGGGCGGTCATAGCAACCCCAGAGCAGCTGTTCGC TGCCTATGAGGATGGATTTGAGCAGTGTGATGCAGGATGGCTGTCTGACCAAACTGTCAGATATCCCATA CGGGCTCCCCGAGAGGGCTGTTATGGAGACATGATGGGGAAGGAAGGGGTCCGGACCTATGGATTCCGCT CTCCCCAGGAAACCTATGATGTGTATTGCTATGTGGATCATCTGGACGGCGATGTGTTCCACATCACTGC TCCCAGTAAATTCACCTTCGAGGAGGCCGAAGCAGAGTGTGCAAACCGGGATGCCAGGCTGGCGACTGTT GGGGAACTTCACGCAGCTTGGAGGAACGGCTTTGACCAGTGCGATTACGGCTGGCTGTCGGATGCCAGCG TGCGGCACCCTGTGACTGTGGCCAGGGCCCAGTGTGGAGGTGGTCTACTTGGGGTGAGAACCCTGTATCG TTTTGAGAACCAGACATGCTTCCCTCTCCCTGATAGCAGATTTGATGCCTACTGCTTTAAACCTAAACAG AATATATCAGAGGCAACCACCATCGAAACGAATACCCTCGCAGAAACTGCATCACCCAGTTTATCCAAAG AACCACACATGGTACCCGAGAGAGCTACACCAGTCATTCCATTAGTCACCGAATTACCTATTTTTACCAC ACACTTCCCTCCTGCAGGAAATATAGTCAATTCTGAACAAAAATCTGTAATCTACTCTCAGGCTATCACA GACAGAGTAGCCACTGAATCACCCACAGCTGCTGGGAGTCCCATTAACTCTTGGGATGCGGATGACTACT TGCCTTCTGGGTCAGGACCACTTGGAAAGCCAGACATATCTGAAATTAAGGAGGAAGGACTTCAAAGTAC AACTGTCACCTCCCAACATGCTACTGCCTCACACCATGGAATTACAGAGGATACGCAAACACATGAATCA GTTACACAGATTGAACAAATAGAAGTGGGCCCCTTGGTAACATCCATGGAAATCACAAATCACATTTCTT TCAAGGAATTTCTAGAAACCAAAACACCATTGGAATCCACAGAAGTGACCCTGGAACAGCATCAGACCGA CATGCCAACAGTTATCACCAGTCCTGAATTGGCAACCACAAGTCACTATGGAGTCACCTTGAGAGAAGAT GATAGAGAAGATATAGCACTGACAGTAAGATCTGGTCAGAGTACCCGGGTCTTTAGCCAAATACCAGAAG TCATCACGGTATCAAAGACATCAGAAGACACTACCTACTCACAGCTAGGGGACTTAGAATTCGTTTCAAC AGCAACAATAACCATGCTTGGCACAGATGGATCACTGACAGATGAAGGGAAAGAACCCCAAACTGATGAT AAGACGGCCGAAGACGAGTTTGGTCAATCACAACCTACAATCCCCTTCCCATCACAGCATCTTACAGAAG TAGAATCACTTCCATATTCTGGTGACACAATATCAGTAGAGAGAATATCCACAGTTAGTTATCCATCATT ACAAACAGATGTGACACAAGGAAGAGAGAGAACAGAAGTACCAAGGCCAGGGTTAAAAAAAGATCCTTAT GCAGTTGATGAGATACAAGAAAAGATCACCAAAGATCCATTTATAGGTACAATAGAAGAAGGCTTCTCTG GAATGTCTCTCTCTACATCTTCCTCAGAGACTTCAGCGGAAAGGACGGAATCTGTTTCCCCAGCATTGAC AATTGAGAAGTTAACTGTGAAGCCAGCCGTAGCAAGCGATGTGGACGAAATGACAATCCTGACTAGATTA GAAACAGATGTGCCAACGTCTGACGAAGATGTTACAAGTGCTCATTTGACCCATAGTACTTTAAATGTGG AAGTGGTCACAGTATTAAAATGGCCAGGGAATGAAGACAATTCAACTTCAAAGCCTTTACCCCCAACAGA ACGTGCAGGCTTTACAAAATCGCCTCCTGTCTCACTTAGCACTGTAGGAATAGTTGGAAAAGATAAAGAA ACCCCAAGTTTCACAGATGGGGGAGACGAATACACTCTTTCCCTAGATGGTACTCCCAAGCCATTAGAAA AGTTTTCTGAGGAAGATTTAACATCTGGAGAATTTGCAGTTACAATTCCTACATCTATATCAATTGACAG TGCAGAGAAGTCAACTTTGGGAGAGCCTACAACTGGAGACAGGGTTCTGTCCACCACAAGCACAAAAGAT CTGGTCATCAATGCAACTGTGGAAGGAAGTGCTTTGGATGAAGATATGGATGCCTCCAAGCCACTGTTTA CTGCCACCCCATTTGTCCACACCTCCGATGTGGAAGAATCGGCATTTGTTAATTATAGTAGCACCCAGCA GCCCACTACTTATGTAGACATATCCCACACTAGTCCTCTTTCTATAATTCCCAAGACAGAATGGAGTGTA TCGGAAACTTCTGTTCCACTGGAAGATGAGGTTCTAGGCAAATCTGATCAAGATACCCTTGAACAGACAC ATCTAGAAGCAACCATGTCTCCTGAAGCTTTGAGTACAATAGAAGTCACACAGGGAGAAACCCAGGAAGA ACCACAAACACCAGGAATCCCATTTCCTGCTCTCAGTTCTACTGCAGTTATGACCAAGGAGACAACAGCA TTTGAAGAAGAGGGGGAGGGGTCAACATATACCCTCTCTGAAGACAGATTGATGACAGATTCTGAAATAG TCCCAAGTTTAGAAACAACTCCAGTTGGTACATCTTATCCAGGTGGTGCAATGACACAGCAGGGAGTAGA AATGGATACAATGGTAACACAGATGTCAAGCATTAGGCCAACAGTAGTTTTAAGTACTGAGCCAGAAGTA AGTTATGAAGCAGAAGGTAGTAGTCCAATGGAATTTGCATCGACTTTGAAGCCCTTTGGGACTCAAGTCA CACAGCTTGTGGAAGAAACGACTGAGGAAGGGAAAAAGACACCCTTGGATTATACAGATTTAGGTTCAGG GTTATTTGAACAACCGAGAGTTACTGAGCTCCCAGATTTTTCAATGACTCCAAGTGATATCAGTGTCTTC ACTGCCATCGACAGCCTTCACAGAACCACACCCTTGAGGCCACCTTCGCCATTCACTGAGGAACCACACA TCTTTGAGAAGGAGCCCAGTGAGAAAACAACAGGTGACATAATACTTCCTAGAGAATCTGTAACACAGCA CCCTCTGACAACTCTCATGGATATCATAGCCAAGAAAACAGAATCTGATATTGATCACGAGTATCACATG ACCTCAAAGCCTCCTGTAATGCAGCCAACAAGACCATCAGTTGTGGAAAGAAAAACAACTTCCAAACCTC AAGAGTTGTCTACTTCATCACCCCCGGCAGGGACCAAATTCCACCCTGACATAAATGTTTATATTATTGA GGTCAGAGAAAACAAGACAGGACCTGATCTCTGCAAAACAAACCCATGCCTCAATGGAGGCACCTGCTAT CCTACTGAGACTTCCTATGTGTGCACCTGTGCACCTGGCTACAGTGGAGACCAGTGTGAACTGGATTTTG ATGAATGTCACTCTAACCCTTGTCGGAATGGAGCCACCTGTGTGGACGGTCTGAATACATTTAGATGCCT CTGCCTTCCGAGTTATGTCGGTGCACTCTGCGAACAAGACACTGAGACATGCGACTATGGCTGGCACAAA TTCCAAGGGCAATGCTACAAGTACTTTGCTCATCGCCGTACATGGGATGCTGCTGAAAGGGAGTGTCGCC TGCAGGGTGCCCACCTCACAAGCATCCTTTCTCATGAGGAACAAATGTTTGTGAATCGTGTGGGCCATGA TTACCAGTGGATTGGCCTCAATGACAAGATGTTTGAACATGACTTCCGCTGGACTGACGGCAGCGCACTG CAATATGAGAACTGGAGACCCAACCAGCCAGACAGCTTCTTTTCTGCTGGAGAAGACTGCGTTGTGATCA TTTGGCATGAGAATGGCCAGTGGAATGACGTCCCCTGCAACTACCACCTCACCTACACCTGCAAGAAGGG AACAGTTGCTTGCGGCCAACCCCCTGTTGTAGAAAATGCCAAGACCTTTGGAAAGATGAAACCACGTTAT GAAATCAACTCCTTGATTAGATACCACTGCAAAGATGGTTTCATTCAGCGTCACCTTCCAACTATCCGGT GCCTAGGAAATGGGAGATGGGCAATGCCTAAAATAACCTGCATGAACCCATCTGCATACCAAAGGACTTA TTCTAAGAAATACTTAAAAAATTCCTCATCAGTCAAGGACAATTCTATAAATACGTCAAAACATGAGCAT CGCTGGAGCCGGAGGTGGCAGGAAACGAGGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217671 representing NM_001170560 Red=Cloning site Green=Tags(s) MLINMNGILWMCSTLLLTHALHKAKMEENPPVKGSLSGKVILPCHFSTLPTLPPDYNTSEFLRIKWSKIE VDKNGKDIKETTVLVAQDGNIKIGQDYKGRVSVPTHPDDVGDASLTMVKLRASDAGVYRCDVMYGIEDTQ NTMSLAVDGVVFHYRAATSRYTLNFESAQQACLDIGAVIATPEQLFAAYEDGFEQCDAGWLSDQTVRYPI RAPREGCYGDMMGKEGVRTYGFRSPQETYDVYCYVDHLDGDVFHITAPSKFTFEEAEAECANRDARLATV GELHAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTCFPLPDSRFDAYCFKPKQ NISEATTIETNTLAETASPSLSKEPHMVPERATPVIPLVTELPIFTTHFPPAGNIVNSEQKSVIYSQAIT DRVATESPTAAGSPINSWDADDYLPSGSGPLGKPDISEIKEEGLQSTTVTSQHATASHHGITEDTQTHES VTQIEQIEVGPLVTSMEITNHISFKEFLETKTPLESTEVTLEQHQTDMPTVITSPELATTSHYGVTLRED DREDIALTVRSGQSTRVFSQIPEVITVSKTSEDTTYSQLGDLEFVSTATITMLGTDGSLTDEGKEPQTDD KTAEDEFGQSQPTIPFPSQHLTEVESLPYSGDTISVERISTVSYPSLQTDVTQGRERTEVPRPGLKKDPY AVDEIQEKITKDPFIGTIEEGFSGMSLSTSSSETSAERTESVSPALTIEKLTVKPAVASDVDEMTILTRL ETDVPTSDEDVTSAHLTHSTLNVEVVTVLKWPGNEDNSTSKPLPPTERAGFTKSPPVSLSTVGIVGKDKE TPSFTDGGDEYTLSLDGTPKPLEKFSEEDLTSGEFAVTIPTSISIDSAEKSTLGEPTTGDRVLSTTSTKD LVINATVEGSALDEDMDASKPLFTATPFVHTSDVEESAFVNYSSTQQPTTYVDISHTSPLSIIPKTEWSV SETSVPLEDEVLGKSDQDTLEQTHLEATMSPEALSTIEVTQGETQEEPQTPGIPFPALSSTAVMTKETTA FEEEGEGSTYTLSEDRLMTDSEIVPSLETTPVGTSYPGGAMTQQGVEMDTMVTQMSSIRPTVVLSTEPEV SYEAEGSSPMEFASTLKPFGTQVTQLVEETTEEGKKTPLDYTDLGSGLFEQPRVTELPDFSMTPSDISVF TAIDSLHRTTPLRPPSPFTEEPHIFEKEPSEKTTGDIILPRESVTQHPLTTLMDIIAKKTESDIDHEYHM TSKPPVMQPTRPSVVERKTTSKPQELSTSSPPAGTKFHPDINVYIIEVRENKTGPDLCKTNPCLNGGTCY PTETSYVCTCAPGYSGDQCELDFDECHSNPCRNGATCVDGLNTFRCLCLPSYVGALCEQDTETCDYGWHK FQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSAL QYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRY EINSLIRYHCKDGFIQRHLPTIRCLGNGRWAMPKITCMNPSAYQRTYSKKYLKNSSSVKDNSINTSKHEH RWSRRWQETRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001170560
ORF Size	4863 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001170560.1, NP_001164031.1
RefSeq Size	7073 bp
RefSeq ORF	4866 bp
Locus ID	114122
UniProt ID	Q9ERB4
MW	179.2 kDa
Gene Summary	extracellular matrix protein secreted by many cell types; part of the large chondroitin sulfate proteoglycan (CSPG) family [RGD, Feb 2006]

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