Tns1 (NM_001191810) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Tns
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217709 representing NM_001191810 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACCGTGAGCCTGGTGTGCTGCGAGGCGCTCGAGCCTCTGCCTTCTTGTGGCCCGCAGCCCC CCGGGACTCCGCCGGGCCCCGCGCGGCCCGAGCGCTCTGAGCCCGGGGGTGCGGCATCCATCCAGAGGAG GAGGCTGCTGCTTCAGCCAGAAGACCTGGAGGCCCCCAAGACCCACCACTTCAAGGTGAAGGCCTTCAAG AAGGTGAAGCCCTGTGGTGTCTGCCGGCAGGCCATCACTCGGGAAGGCTGTATTTGCAAAGTCTGCAGCT TCTCCTGTCATCGGAAATGCCAGGCCAAGGTGGCTGCCCCTTGCGTTCCTCCATCCAGCCATGAACTGGT GCCCATCGCCACGGAGAGTGTGCCCAAGAATGTAGTAGATGTGGGGGAAGGAGACTGCCGGGGTGGAAGT ACTCCGAAAAACCTGGAGGAGGCCGGCTCCATGAGAGTTTCTCCAAGCATACAGCCACAGCCACAGCCAC AGCCACAGCCTATCAGTCTCTCTAGAAACCTGAGTGTGAGCCGAGCCATGGAAGACAGCTGTGAGCTGGA CCTGGTGTATATCACCGAACGCATCATCGCCGTGTCCTTCCCCAGCACAGCTAATGAGGAGAACTTCCGC AGCAACCTCCGTGAAGTGGCTCAGATGCTGAAGTCCAAGCATGGGGGCAACTACCTGCTATTCAACCTCT CTGAGCAGAGGCCTGACATCACAAAGCTCCATGCTAAGGTCCTGGAATTTGGCTGGCCTGACCTGCACAC ACCAGCTCTGGAGAAGATATGCAGTGTCTGTAAGGCCATGGACACCTGGCTCAATGCAGACCCCCACAAT GTCGTCGTTCTCCACAACAAGGGAAACCGAGGAAGGATTGGGGTCGTCATTGCAGCATATATGCACTACA GCAACATTTCAGCCAGTGCTGACCAGGCTCTTGACAGGTTTGCAATGAAGAGATTCTATGAGGACAAGAT CGTGCCCATTGGCCAGCCATCCCAGAGGAGATATGTGCACTACTTCAGCGGCCTGCTCTCCGGCTCCATC AAAATGAACAACAAGCCTTTGTTTCTGCACCATGTTATCATGCACGGCATCCCCAACTTTGAGTCTAAAG GAGGGTGCCGGCCCTTTCTTCGCATCTACCAAGCCATGCAACCTGTGTACACATCTGGCATTTACAACAT CCCTGGAGACAGCCAGGCCAGCATCTGTATCACCATCGAACCAGGGCTGCTCCTGAAAGGAGACATCTTG CTGAAGTGTTATCATAAGAAGTTCCGGAGCCCGGCTCGAGACGTCATCTTCCGAGTGCAGTTCCACACCT GTGCCATCCATGACTTGGGGGTCGTTTTTGGGAAGGAAGATCTGGATGAGGCCTTCAAAGATGATCGATT TCCTGACTATGGCAAAGTGGAGTTTGTCTTTTCCTATGGACCAGAGAAAATCCAAGGCATGGAGCACCTG GAGAACGGGCCCAGTGTATCTGTAGACTATAACACTTCCGACCCCCTCATCCGATGGGATTCCTATGACA ACTTCAATGGGCATCGTGAGGATGGCATGGAAGAAGTGGTGGGACACACCCAAGGGCCGCTGGATGGGAG CCTGTATGCCAAAGTGAAGAAGAAGGACTCCCTGAATGGCAGTTCGGGGCCTATCACCACCGCAAGGCCT GCCCTGTCAGCCACTCCCAACCATGTGGAACACACACTGTCTGTGAGCAGCGACTCCGGCAACTCCACAG CCTCCACTAAGACAGACAAGACTGACGAGCCAGTCTCTGGGGCTGCCATTGCCCCTGCTGCCCTGAGTCC TCAGGAGAAGAAAGAGCTGGATCGCCTACTAAGTGGCTTCGGTGTAGATAGAGAGAAGCAAGGTGCCATG TACCGGGCTCAGCAACTCAGATCCCACCCAGGCGGGGGCCCGACTGTGTCCTCCCCTGGCCGCCACATTG TCCCAGCTCAGGTTCATGTCAATGGTGGGGCCCTGGCATCTGAGCGGGAGACGGACATTCTGGATGATGA GCTGCCCATCCAGGATGGGCAGAGTGGGGGCAGCATGGGAACGCTGTCTTCCCTGGATGGGGTCACCAAC ACCAGCGAAGGTGGCTACCCAGAGACCCTGTCCCCACTGACCAATGGTCTGGACAAACCCTATCCCACAG AACCTATTCTCAACGGGGGAGGCTACCCCTATGAGTCTGCCAACCGGGTGGTACCTGTTCATAGCAGTCA CTCGGCTCCCATTCGACCCTCCTACTCTGCTCAGGAGGGTTTAGGTGGCTACCAGCGTGAGGGACCCCAC CCGGCCTGGTCAAAACAAGTGACCAGCGCTCACCGTGGCTATGACCCCAGTGGTCTATTCCGATCCCAGT CCTTTCCAGATGTGGAGCCTCAGCTGCCCCAAGCTCCAGCCCGTGGGGGCAGCAGCCGAGAGGCTGTGCA GAGGGGCCTGAATTCATGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCCCCACCCACCTCCCCGCCAGCAGGAGCGATCCCCACTGCAGAGTCTAGCCTGCAGCA AGCCTAGCCCCCATCTGTCAGCAGAGACCCCCATACCAGCTCTCCCAGAGTTCCCAAGGGCAGCCTCCCA GCAGGAGATTGAGCAGTCCATCGAAACTCTCAATATGTTGATGCTGGACTTGGAGCCAGCCTCAGCTGCT GCCCCTCTTCATAAGTCCCAGAGTGTCCCTGGGGCCTGGCCAGGGGCTTCCCCACTCTCTTCCCAGCCCC TCTTGGGGTCTTCCTGCCAGTCCCACCCACTGACCCAGTCCAGATCTGGCTATATCCCCAGTGGGGACAC CCTGGGAGCCCCGGAGCTGCTCTCTTCTGGAAGGTCTTACTCACCTTACGATTATCAGCTGCATCCAGCT GCATCCAACCAGAGTTTCCGGCCAAAAAGTCCAGCCTCTTCCACCTTCCTTCCAAGCCCTCATAGCTCTG CAGGGCCTCAGGAGCCCCCAGCCTCTCTCCCTGGTCTCATCGCCGAGCCTCAGCTCCCACTGAAGGAAAC TACTTCAGATACCTCCCGAACTCCAGAGGAGGAGCCGCTGAACCTGGAAGGGCTGGTAGCCCATCGGGTA GCAGGGGTTCAGGCTCGGGAGAGGCAGCCTGCAGAGCCCCCAGCCCCACTCCGGAGGCGAGCAGCCAGTG ATGGACAGTATGAGAACCAGTCTCCGGAGGCCACATCCCCCCGTAGTCCTGGGGTCCGCTCCCCCGTCCA GTGCGTCTCCCCTGAGTTGGCTCTTACCATTGCCCTCAATCCTGGAGGGCGCCCCAAAGAGCCCCATTTG CATAGCTACAAGGAGGCCTTTGAGGAGATGGAGGGGACCTCCCCAAGCAGCCCACCACCCAGCGTGGCAC GTTCTCCTCCAGGTCTGGCTAAGACACCCCTGTCTGCTCTGGGCCTGAAACCCCACAACCCAGCGGACAT CTTGCTGCACCCCACGGGTGTTGCCAGAAGACTGATCCAGCCAGAGGAAGATGAGGGAGAGGAGGTAGCT AAGCCTCCAGAAGAGCCCCGGAGCTATGTGGAGTCTGTTGCAAGGACAGCAGTGGCAGGCCCTCGAGCTC AGGACGTGGAGCCTAAGAGCTTCAGTGCACCAGCCACTCATGCCTATGGCCACGAGACACCCCTGAGAAA CGGGACTCCCGGAGGCTCCTTTGTCTCCCCCAGCCCCCTTTCCACAAGCAGTCCCATCCTCAGTGCTGAC AGCACTTCCGTGGGCAGTTTCCCATCAGCGGGAAGCAGTGACCAGGGTCCCCGGACACCCATCCAGCCCA TGCTGGACTCCAGCATTCGCTCAGGCAGCTTGGGGCAGCCGAGCCCTGCAGCCTTGAGTTATCAGAGCTC CTCACCTGTCCCTGTTGGTGGCAGCAGCTACAGCAGCCCTGACTACTCCCTTCAACCATTCAGCTCTTCT CCAGAAAGCCAGGGCCAGCCACATTTCAGTGCGGCCAGCGTCCACATGGTGCCTGGGAGCCCTCAGGCAC GACACAGGACAGTGGGCACCAACACTCCACCTAGTCCCGGCTTTGGCCGGCGGGCCATCAACCCCACCAT GGCTGCCCCTGGTAGTCCCAGTTTGAGCCACCGACAGGTGATGGGTCCATCTGGTCCAGGTTTCCATGGT AACGTGGTTTCTGGCCACCCAACCAGTGCAACGGTTGCTCCTGGAAGTCCCAGCTTGGGCCGGCACCCAG TGGGAAGCCATCAAGTTCCAGGTCTCCACAGCGGTGTGGTCACTACTCCAGGGAGCCCAAGCCTGGGCCG ACACCCTGGGGCCCACCAAGGGAATCTGGTCTCCAGTCTCCATGGCAATGCAGTCATTAGCCCTGGGAGC CCCAGTTTGGGCCGGCACCTGGGTGGGTCTGGATCTGTGGTTCCTGGAAGCCCCAGCTTGGACCGGCATG TAGCTTATGGTGGCTACTCTACCCCTGAGGACCGAAGACCAACACTGTCCCGGCAAAGCAGTGCCTCAGG CTACCAGGCTCCTTCCACACCGTCATTCCCTGTTTCTCCTGCCTACTACCCTGGCCTGAGCAGCCCTGCC ACCTCACCCTCGCCAGACTCAGCAGCCTTCCGGCAAGGGAGCCCCACACCAGCCTTACCAGAGAAGCGGA GGATGTCAGTGGGAGACCGGGCAGGCAGTCTCCCCAACTATGCTACCATCAATGGGAAAGTGTCCTCCTC GCCTGTCGCCAATGGCATGGCCAGCCCCAGTGGGAGCAGCACCGTTTCCTTCTCTCACACGCTTCCAGAC TTTTCCAAGTACTCGATGCCAGATAATAGTCCGGAGACACGGGCTAAAGTGAAGTTTGTCCAGGATACTT CCAAGTATTGGTACAAGCCTGAGATCTCCAGAGAGCAGGCCATTGCACTCCTGAAGGATCAGGAGCCAGG GGCCTTCATCATCCGAGACAGCCACTCCTTCCGAGGGGCTTATGGACTGGCCATGAAGGTGTCCTCACCC CCTCCAACCATTACTCAGCAGGGCAAGAAAGGAGACATGACCCATGAGCTGGTGAGGCACTTTCTGATCG AGACAGGCCCTAGAGGAGTCAAGCTCAAGGGCTGTCCCAATGAGCCAAACTTTGGCTCCCTTTCTGCCCT CGTCTATCAGCACTCCGTCATCCCACTGGCCCTGCCCTGTAAGCTGGTCATTCCAAGCCGAGACCCCACA GATGAATCAAAAGATAGCTCAGGCCCTGCCAACTCAACCACTGACCTGCTGAAGCAAGGAGCAGCTTGCA ATGTGCTCTTCATCAATTCTGTGGATATGGAGTCACTCACCGGGCCACAGGCAATTTCCAAAGCCACATC TGAGACTTTGGCTGCTGACCCCACGCCAGCTGCCACCATTGTTCACTTCAAGGTCTCTGCCCAAGGAATC ACACTGACTGACAATCAAAGAAAGCTCTTCTTCAGACGACATTACCCCCTCAACACTGTCACCTTCTGTG ACCTGGATCCACAAGAAAGGAAGTGGATGAAGACAGAGGGAGGTGCCCCTGCTAAGCTCTTCGGCTTCGT GGCCCGGAAGCAGGGCAGCACCACAGACAACGCTTGCCACCTCTTCGCAGAGCTTGACCCCAACCAGCCT GCGTCTGCCATTGTCAACTTCGTCTCCAAGGTCATGCTGAGTGCTGGCCAAAAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191810
Insert Size	5730 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191810.1, NP_001178739.1
RefSeq Size	6126 bp
RefSeq ORF	5730 bp
Locus ID	301509
Gene Summary	mouse homolog is a focal adhesion phosphoprotein that binds to F-actin; knockout mice develop progessive kidney degeneration [RGD, Feb 2006]

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