Tns1 (NM_001191810) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Tns
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217709 representing NM_001191810 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACCGTGAGCCTGGTGTGCTGCGAGGCGCTCGAGCCTCTGCCTTCTTGTGGCCCGCAGCCCC CCGGGACTCCGCCGGGCCCCGCGCGGCCCGAGCGCTCTGAGCCCGGGGGTGCGGCATCCATCCAGAGGAG GAGGCTGCTGCTTCAGCCAGAAGACCTGGAGGCCCCCAAGACCCACCACTTCAAGGTGAAGGCCTTCAAG AAGGTGAAGCCCTGTGGTGTCTGCCGGCAGGCCATCACTCGGGAAGGCTGTATTTGCAAAGTCTGCAGCT TCTCCTGTCATCGGAAATGCCAGGCCAAGGTGGCTGCCCCTTGCGTTCCTCCATCCAGCCATGAACTGGT GCCCATCGCCACGGAGAGTGTGCCCAAGAATGTAGTAGATGTGGGGGAAGGAGACTGCCGGGGTGGAAGT ACTCCGAAAAACCTGGAGGAGGCCGGCTCCATGAGAGTTTCTCCAAGCATACAGCCACAGCCACAGCCAC AGCCACAGCCTATCAGTCTCTCTAGAAACCTGAGTGTGAGCCGAGCCATGGAAGACAGCTGTGAGCTGGA CCTGGTGTATATCACCGAACGCATCATCGCCGTGTCCTTCCCCAGCACAGCTAATGAGGAGAACTTCCGC AGCAACCTCCGTGAAGTGGCTCAGATGCTGAAGTCCAAGCATGGGGGCAACTACCTGCTATTCAACCTCT CTGAGCAGAGGCCTGACATCACAAAGCTCCATGCTAAGGTCCTGGAATTTGGCTGGCCTGACCTGCACAC ACCAGCTCTGGAGAAGATATGCAGTGTCTGTAAGGCCATGGACACCTGGCTCAATGCAGACCCCCACAAT GTCGTCGTTCTCCACAACAAGGGAAACCGAGGAAGGATTGGGGTCGTCATTGCAGCATATATGCACTACA GCAACATTTCAGCCAGTGCTGACCAGGCTCTTGACAGGTTTGCAATGAAGAGATTCTATGAGGACAAGAT CGTGCCCATTGGCCAGCCATCCCAGAGGAGATATGTGCACTACTTCAGCGGCCTGCTCTCCGGCTCCATC AAAATGAACAACAAGCCTTTGTTTCTGCACCATGTTATCATGCACGGCATCCCCAACTTTGAGTCTAAAG GAGGGTGCCGGCCCTTTCTTCGCATCTACCAAGCCATGCAACCTGTGTACACATCTGGCATTTACAACAT CCCTGGAGACAGCCAGGCCAGCATCTGTATCACCATCGAACCAGGGCTGCTCCTGAAAGGAGACATCTTG CTGAAGTGTTATCATAAGAAGTTCCGGAGCCCGGCTCGAGACGTCATCTTCCGAGTGCAGTTCCACACCT GTGCCATCCATGACTTGGGGGTCGTTTTTGGGAAGGAAGATCTGGATGAGGCCTTCAAAGATGATCGATT TCCTGACTATGGCAAAGTGGAGTTTGTCTTTTCCTATGGACCAGAGAAAATCCAAGGCATGGAGCACCTG GAGAACGGGCCCAGTGTATCTGTAGACTATAACACTTCCGACCCCCTCATCCGATGGGATTCCTATGACA ACTTCAATGGGCATCGTGAGGATGGCATGGAAGAAGTGGTGGGACACACCCAAGGGCCGCTGGATGGGAG CCTGTATGCCAAAGTGAAGAAGAAGGACTCCCTGAATGGCAGTTCGGGGCCTATCACCACCGCAAGGCCT GCCCTGTCAGCCACTCCCAACCATGTGGAACACACACTGTCTGTGAGCAGCGACTCCGGCAACTCCACAG CCTCCACTAAGACAGACAAGACTGACGAGCCAGTCTCTGGGGCTGCCATTGCCCCTGCTGCCCTGAGTCC TCAGGAGAAGAAAGAGCTGGATCGCCTACTAAGTGGCTTCGGTGTAGATAGAGAGAAGCAAGGTGCCATG TACCGGGCTCAGCAACTCAGATCCCACCCAGGCGGGGGCCCGACTGTGTCCTCCCCTGGCCGCCACATTG TCCCAGCTCAGGTTCATGTCAATGGTGGGGCCCTGGCATCTGAGCGGGAGACGGACATTCTGGATGATGA GCTGCCCATCCAGGATGGGCAGAGTGGGGGCAGCATGGGAACGCTGTCTTCCCTGGATGGGGTCACCAAC ACCAGCGAAGGTGGCTACCCAGAGACCCTGTCCCCACTGACCAATGGTCTGGACAAACCCTATCCCACAG AACCTATTCTCAACGGGGGAGGCTACCCCTATGAGTCTGCCAACCGGGTGGTACCTGTTCATAGCAGTCA CTCGGCTCCCATTCGACCCTCCTACTCTGCTCAGGAGGGTTTAGGTGGCTACCAGCGTGAGGGACCCCAC CCGGCCTGGTCAAAACAAGTGACCAGCGCTCACCGTGGCTATGACCCCAGTGGTCTATTCCGATCCCAGT CCTTTCCAGATGTGGAGCCTCAGCTGCCCCAAGCTCCAGCCCGTGGGGGCAGCAGCCGAGAGGCTGTGCA GAGGGGCCTGAATTCATGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG CAGCAGCAGCAGCCCCACCCACCTCCCCGCCAGCAGGAGCGATCCCCACTGCAGAGTCTAGCCTGCAGCA AGCCTAGCCCCCATCTGTCAGCAGAGACCCCCATACCAGCTCTCCCAGAGTTCCCAAGGGCAGCCTCCCA GCAGGAGATTGAGCAGTCCATCGAAACTCTCAATATGTTGATGCTGGACTTGGAGCCAGCCTCAGCTGCT GCCCCTCTTCATAAGTCCCAGAGTGTCCCTGGGGCCTGGCCAGGGGCTTCCCCACTCTCTTCCCAGCCCC TCTTGGGGTCTTCCTGCCAGTCCCACCCACTGACCCAGTCCAGATCTGGCTATATCCCCAGTGGGGACAC CCTGGGAGCCCCGGAGCTGCTCTCTTCTGGAAGGTCTTACTCACCTTACGATTATCAGCTGCATCCAGCT GCATCCAACCAGAGTTTCCGGCCAAAAAGTCCAGCCTCTTCCACCTTCCTTCCAAGCCCTCATAGCTCTG CAGGGCCTCAGGAGCCCCCAGCCTCTCTCCCTGGTCTCATCGCCGAGCCTCAGCTCCCACTGAAGGAAAC TACTTCAGATACCTCCCGAACTCCAGAGGAGGAGCCGCTGAACCTGGAAGGGCTGGTAGCCCATCGGGTA GCAGGGGTTCAGGCTCGGGAGAGGCAGCCTGCAGAGCCCCCAGCCCCACTCCGGAGGCGAGCAGCCAGTG ATGGACAGTATGAGAACCAGTCTCCGGAGGCCACATCCCCCCGTAGTCCTGGGGTCCGCTCCCCCGTCCA GTGCGTCTCCCCTGAGTTGGCTCTTACCATTGCCCTCAATCCTGGAGGGCGCCCCAAAGAGCCCCATTTG CATAGCTACAAGGAGGCCTTTGAGGAGATGGAGGGGACCTCCCCAAGCAGCCCACCACCCAGCGTGGCAC GTTCTCCTCCAGGTCTGGCTAAGACACCCCTGTCTGCTCTGGGCCTGAAACCCCACAACCCAGCGGACAT CTTGCTGCACCCCACGGGTGTTGCCAGAAGACTGATCCAGCCAGAGGAAGATGAGGGAGAGGAGGTAGCT AAGCCTCCAGAAGAGCCCCGGAGCTATGTGGAGTCTGTTGCAAGGACAGCAGTGGCAGGCCCTCGAGCTC AGGACGTGGAGCCTAAGAGCTTCAGTGCACCAGCCACTCATGCCTATGGCCACGAGACACCCCTGAGAAA CGGGACTCCCGGAGGCTCCTTTGTCTCCCCCAGCCCCCTTTCCACAAGCAGTCCCATCCTCAGTGCTGAC AGCACTTCCGTGGGCAGTTTCCCATCAGCGGGAAGCAGTGACCAGGGTCCCCGGACACCCATCCAGCCCA TGCTGGACTCCAGCATTCGCTCAGGCAGCTTGGGGCAGCCGAGCCCTGCAGCCTTGAGTTATCAGAGCTC CTCACCTGTCCCTGTTGGTGGCAGCAGCTACAGCAGCCCTGACTACTCCCTTCAACCATTCAGCTCTTCT CCAGAAAGCCAGGGCCAGCCACATTTCAGTGCGGCCAGCGTCCACATGGTGCCTGGGAGCCCTCAGGCAC GACACAGGACAGTGGGCACCAACACTCCACCTAGTCCCGGCTTTGGCCGGCGGGCCATCAACCCCACCAT GGCTGCCCCTGGTAGTCCCAGTTTGAGCCACCGACAGGTGATGGGTCCATCTGGTCCAGGTTTCCATGGT AACGTGGTTTCTGGCCACCCAACCAGTGCAACGGTTGCTCCTGGAAGTCCCAGCTTGGGCCGGCACCCAG TGGGAAGCCATCAAGTTCCAGGTCTCCACAGCGGTGTGGTCACTACTCCAGGGAGCCCAAGCCTGGGCCG ACACCCTGGGGCCCACCAAGGGAATCTGGTCTCCAGTCTCCATGGCAATGCAGTCATTAGCCCTGGGAGC CCCAGTTTGGGCCGGCACCTGGGTGGGTCTGGATCTGTGGTTCCTGGAAGCCCCAGCTTGGACCGGCATG TAGCTTATGGTGGCTACTCTACCCCTGAGGACCGAAGACCAACACTGTCCCGGCAAAGCAGTGCCTCAGG CTACCAGGCTCCTTCCACACCGTCATTCCCTGTTTCTCCTGCCTACTACCCTGGCCTGAGCAGCCCTGCC ACCTCACCCTCGCCAGACTCAGCAGCCTTCCGGCAAGGGAGCCCCACACCAGCCTTACCAGAGAAGCGGA GGATGTCAGTGGGAGACCGGGCAGGCAGTCTCCCCAACTATGCTACCATCAATGGGAAAGTGTCCTCCTC GCCTGTCGCCAATGGCATGGCCAGCCCCAGTGGGAGCAGCACCGTTTCCTTCTCTCACACGCTTCCAGAC TTTTCCAAGTACTCGATGCCAGATAATAGTCCGGAGACACGGGCTAAAGTGAAGTTTGTCCAGGATACTT CCAAGTATTGGTACAAGCCTGAGATCTCCAGAGAGCAGGCCATTGCACTCCTGAAGGATCAGGAGCCAGG GGCCTTCATCATCCGAGACAGCCACTCCTTCCGAGGGGCTTATGGACTGGCCATGAAGGTGTCCTCACCC CCTCCAACCATTACTCAGCAGGGCAAGAAAGGAGACATGACCCATGAGCTGGTGAGGCACTTTCTGATCG AGACAGGCCCTAGAGGAGTCAAGCTCAAGGGCTGTCCCAATGAGCCAAACTTTGGCTCCCTTTCTGCCCT CGTCTATCAGCACTCCGTCATCCCACTGGCCCTGCCCTGTAAGCTGGTCATTCCAAGCCGAGACCCCACA GATGAATCAAAAGATAGCTCAGGCCCTGCCAACTCAACCACTGACCTGCTGAAGCAAGGAGCAGCTTGCA ATGTGCTCTTCATCAATTCTGTGGATATGGAGTCACTCACCGGGCCACAGGCAATTTCCAAAGCCACATC TGAGACTTTGGCTGCTGACCCCACGCCAGCTGCCACCATTGTTCACTTCAAGGTCTCTGCCCAAGGAATC ACACTGACTGACAATCAAAGAAAGCTCTTCTTCAGACGACATTACCCCCTCAACACTGTCACCTTCTGTG ACCTGGATCCACAAGAAAGGAAGTGGATGAAGACAGAGGGAGGTGCCCCTGCTAAGCTCTTCGGCTTCGT GGCCCGGAAGCAGGGCAGCACCACAGACAACGCTTGCCACCTCTTCGCAGAGCTTGACCCCAACCAGCCT GCGTCTGCCATTGTCAACTTCGTCTCCAAGGTCATGCTGAGTGCTGGCCAAAAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217709 representing NM_001191810 Red=Cloning site Green=Tags(s) MGCTVSLVCCEALEPLPSCGPQPPGTPPGPARPERSEPGGAASIQRRRLLLQPEDLEAPKTHHFKVKAFK KVKPCGVCRQAITREGCICKVCSFSCHRKCQAKVAAPCVPPSSHELVPIATESVPKNVVDVGEGDCRGGS TPKNLEEAGSMRVSPSIQPQPQPQPQPISLSRNLSVSRAMEDSCELDLVYITERIIAVSFPSTANEENFR SNLREVAQMLKSKHGGNYLLFNLSEQRPDITKLHAKVLEFGWPDLHTPALEKICSVCKAMDTWLNADPHN VVVLHNKGNRGRIGVVIAAYMHYSNISASADQALDRFAMKRFYEDKIVPIGQPSQRRYVHYFSGLLSGSI KMNNKPLFLHHVIMHGIPNFESKGGCRPFLRIYQAMQPVYTSGIYNIPGDSQASICITIEPGLLLKGDIL LKCYHKKFRSPARDVIFRVQFHTCAIHDLGVVFGKEDLDEAFKDDRFPDYGKVEFVFSYGPEKIQGMEHL ENGPSVSVDYNTSDPLIRWDSYDNFNGHREDGMEEVVGHTQGPLDGSLYAKVKKKDSLNGSSGPITTARP ALSATPNHVEHTLSVSSDSGNSTASTKTDKTDEPVSGAAIAPAALSPQEKKELDRLLSGFGVDREKQGAM YRAQQLRSHPGGGPTVSSPGRHIVPAQVHVNGGALASERETDILDDELPIQDGQSGGSMGTLSSLDGVTN TSEGGYPETLSPLTNGLDKPYPTEPILNGGGYPYESANRVVPVHSSHSAPIRPSYSAQEGLGGYQREGPH PAWSKQVTSAHRGYDPSGLFRSQSFPDVEPQLPQAPARGGSSREAVQRGLNSWQQQQQQQQQQQQQQQQQ QQQQPHPPPRQQERSPLQSLACSKPSPHLSAETPIPALPEFPRAASQQEIEQSIETLNMLMLDLEPASAA APLHKSQSVPGAWPGASPLSSQPLLGSSCQSHPLTQSRSGYIPSGDTLGAPELLSSGRSYSPYDYQLHPA ASNQSFRPKSPASSTFLPSPHSSAGPQEPPASLPGLIAEPQLPLKETTSDTSRTPEEEPLNLEGLVAHRV AGVQARERQPAEPPAPLRRRAASDGQYENQSPEATSPRSPGVRSPVQCVSPELALTIALNPGGRPKEPHL HSYKEAFEEMEGTSPSSPPPSVARSPPGLAKTPLSALGLKPHNPADILLHPTGVARRLIQPEEDEGEEVA KPPEEPRSYVESVARTAVAGPRAQDVEPKSFSAPATHAYGHETPLRNGTPGGSFVSPSPLSTSSPILSAD STSVGSFPSAGSSDQGPRTPIQPMLDSSIRSGSLGQPSPAALSYQSSSPVPVGGSSYSSPDYSLQPFSSS PESQGQPHFSAASVHMVPGSPQARHRTVGTNTPPSPGFGRRAINPTMAAPGSPSLSHRQVMGPSGPGFHG NVVSGHPTSATVAPGSPSLGRHPVGSHQVPGLHSGVVTTPGSPSLGRHPGAHQGNLVSSLHGNAVISPGS PSLGRHLGGSGSVVPGSPSLDRHVAYGGYSTPEDRRPTLSRQSSASGYQAPSTPSFPVSPAYYPGLSSPA TSPSPDSAAFRQGSPTPALPEKRRMSVGDRAGSLPNYATINGKVSSSPVANGMASPSGSSTVSFSHTLPD FSKYSMPDNSPETRAKVKFVQDTSKYWYKPEISREQAIALLKDQEPGAFIIRDSHSFRGAYGLAMKVSSP PPTITQQGKKGDMTHELVRHFLIETGPRGVKLKGCPNEPNFGSLSALVYQHSVIPLALPCKLVIPSRDPT DESKDSSGPANSTTDLLKQGAACNVLFINSVDMESLTGPQAISKATSETLAADPTPAATIVHFKVSAQGI TLTDNQRKLFFRRHYPLNTVTFCDLDPQERKWMKTEGGAPAKLFGFVARKQGSTTDNACHLFAELDPNQP ASAIVNFVSKVMLSAGQKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191810
ORF Size	5727 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191810.1, NP_001178739.1
RefSeq Size	6126 bp
RefSeq ORF	5730 bp
Locus ID	301509
MW	203.9 kDa
Gene Summary	mouse homolog is a focal adhesion phosphoprotein that binds to F-actin; knockout mice develop progessive kidney degeneration [RGD, Feb 2006]

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