Chd5 (NM_001271226) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	CHD-5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217713 representing NM_001271226 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGGCCCGCTGGGCACCGAGGAGGAGCTGCCGCGGCTGTTCGCGGAGGAGATGGAGAACGAGGAGG AGATGTCAGAAGAAGAAGACGGTGGCCTTGAAGGCTTTGAGGACTTCTTTCCTGCAGAGCCCGTGAGCCT TCCCAAGAAGAAGCCGAAGAAGCTGAAGGAGAGCAAGTCCAAAGGGAAGAGGAAGAAGAAAGAGGGGAGC AATGATGAGCTGTCAGAAAATGAGGAGGATCTGGAGGAGAAGTCAGAGAGCGAAGGTAGCGACTACAGCC CCACCAAGAAGAAGAAGAAGAAACTGAAGGAGAAAAAGGAGAAGAAGGCTAAGCGGAAAAAGAGGGATGA GGATGAAGAGGATAATGAGGACGGAGGCTTGAAGGAGCCCAAGTCCTCGGGACAGCTCATGGCCGAATGG GGGCTTGACGATGTGGATTACCTATTTTCTGAGGATGACTACCACACGCTGACCAACTACAAGGCCTTCA GCCAGTTCCTCCGGCCTCTTATTGCTAAGAAGAACCCGAAGATCCCCATGTCCAAAATGATGACTGTCCT GGGGGCCAAGTGGCGAGAATTCAGCGCCAACAACCCATTCAAGGGCAGCTCGGCGGCGGCAGCTGCGGCG GCCGTGGCTGCGGCCGTGGAGACGGTGACCATCGCTCCTCCACTGGCCATCAGTCCCCAGCAAGTGCCCC AGCCTCTGCCCGTACGCAAGGCCAAGACCAAAGAGGGCAAGGGGCCCGGAGTGAGGAAGAAGAACAAAGG CGCCAAAGATTCCAAGAAAAAGGGCAGGGGCAAGAGAGTGGCAGGGCTCAAGTTCCGCTTCGGAGGGATC AGCAAGAGGAAGAAGGGTTCCTCGAGCGAGGAGGATGAACCGGAGGACTCAGATTTGGACAATGCCAGCA TCCACAGCTCTTCTGTGCGTTCTGAGTGCTCTGCAGCTCTGGGCAAGAAGAATAAGAGGAGGCGCAAGAA GAAGAGGATTGATGATGGTGACGGCTATGAGACAGACCACCAGGACTACTGCGAGGTGTGCCAGCAGGGA GGGGAGATCATTCTCTGTGATACCTGCCCGAGGGCGTACCACCTGGTCTGCCTGGACCCGGAGCTGGAGA AGGCTCCAGAGGGCAAGTGGAGCTGCCCACACTGTGAGAAGGAGGGGATCCAGTGGGAGCCGAAGGATGA TGATGAGGAAGAGGAGGAGGGCGGTTGCGAGGAGGAGGAGGATGACCACATGGAGTTCTGCCGCGTGTGC AAGGACGGTGGCGAGCTTCTGTGCTGTGATGCTTGTCCCTCCTCCTACCACTTGCACTGCCTCAACCCGC CGCTGCCGGAGATCCCGAACGGTGAATGGCTCTGCCCGCGCTGTACATGTCCCCCACTGAAGGGCAAGGT TCAGCGGATCCTACACTGGAGGTGGACGGAACCCCCGGCTCCCTTTATGGTGGGGCTGCCAGGACCAGAG GTGGAGCCCGGTATGCCCCCGCCCAGGCCTCTGGAGGGCATACCTGAGAGAGAGTTCTTTGTCAAGTGGG CTGGTCTCTCCTACTGGCACTGCTCCTGGGTGAAGGAGCTACAGTTGGAGCTGTACCACACCGTGATGTA TCGCAACTACCAAAGAAAGAATGACATGGACGAACCACCACCCTTCGATTACGGCTCTGGTGATGAGGAC GGTAAGAGCGAGAAACGGAAGAACAAGGACCCTCTCTACGCCAAGATGGAGGAACGCTTCTACCGCTACG GCATCAAGCCCGAGTGGATGATGGTCCACCGCATCCTGAACCACAGCTTTGACAAGAAGGGGGATGTACA TTACCTGATCAAGTGGAAGGACCTGCCCTACGACCAGTGCACCTGGGAGATTGACGAGATTGACATCCCC TACTATGACAACCTGAAGCAGACCTACTGGGGCCACAGGGAACTGATGCTGGGAGAGGACGCTAGGCTGC CCAAGAGGCTGGTCAAGAAGGGCAAGAAGCTGAAGGATGATAAACAAGAGAAGCCACCCGATACACCCAT CGTGGACCCCACAGTCAAGTTCGACAAGCAGCCGTGGTACATCGACTCCACAGGAGGCACGCTGCACCCT TACCAGCTGGAGGGCCTCAACTGGTTACGCTTCTCCTGGGCCCAGGGCACCGACACTATCCTGGCTGATG AGATGGGTTTGGGGAAGACAGTACAGACCATTGTGTTTCTCTATTCTCTGTACAAGGAGGGCCACTCCAA GGGGCCTTACCTGGTCAGCGCGCCCCTGTCCACCATCATCAACTGGGAACGGGAGTTTGAGATGTGGGCT CCTGACTTTTACGTGGTCACCTACACGGGGGACAAGGAGAGCCGCTCTGTGATCCGTGAGAACGAGTTTT CTTTTGAGGACAACGCGATTCGAGGCGGGAAGAAAGTATTCCGCATGAAGAAGGAGGTGCAGATCAAATT CCACGTGTTGCTTACCTCCTATGAGCTCATCACCATTGACCAAGCCATCCTGGGCTCCATCGAGTGGGCC TGCCTCGTGGTGGATGAGGCCCATCGGCTCAAGAACAACCAGTCCAAGTTCTTTAGGGTCTTGAATAGCT ACAAGATCGACTACAAGCTGCTGCTGACGGGGACCCCCCTCCAGAACAACCTGGAGGAGCTGTTCCACCT CCTCAACTTCCTGACTCCAGAGAGGTTCAACAATCTGGAAGGCTTCTTGGAGGAGTTTGCCGACATCTCC AAGGAAGATCAGATCAAGAAGCTGCATGACCTGCTGGGGCCACACATGCTTCGGCGGCTCAAGGCCGACG TGTTCAAGAACATGCCGGCCAAGACGGAGCTGATTGTCCGCGTGGAGCTGAGCCAGATGCAGAAGAAGTA CTACAAGTTCATCCTGACGCGGAACTTCGAGGCGCTCAACTCCAAGGGGGGTGGCAACCAGGTGTCTCTG CTCAACATCATGATGGATCTGAAGAAGTGCTGCAACCACCCGTACCTCTTCCCCGTGGCTGCCGTGGAAG CCCCCATGTTGCCCAACGGCTCCTATGACGGCAGCTCCCTGGTCAAGTCTTCAGGGAAGCTGATGCTACT TCAGAAGATGCTGAAGAAGCTGCGGGATGAGGGGCACCGAGTGCTCATCTTCTCCCAGATGACCAAGATG TTGGACCTGTTGGAGGACTTCCTGGAGTATGAGGGCTACAAGTATGAGCGGATCGACGGGGGTATCACTG GGGGCCTCCGGCAAGAAGCCATCGACAGATTCAATGCTCCTGGGGCCCAACAGTTCTGCTTCCTGCTCTC AACACGTGCCGGTGGCCTGGGCATCAATCTGGCCACGGCCGACACAGTCATCATTTACGACTCGGACTGG AACCCCCACAATGACATCCAGGCCTTCAGCCGTGCTCACCGCATCGGGCAGAACAAGAAGGTCATGATCT ACCGCTTCGTGACACGGGCCTCGGTGGAGGAACGCATCACTCAGGTGGCCAAGCGCAAGATGATGCTCAC GCACCTGGTGGTGCGACCCGGCCTGGGCTCCAAGTCGGGCTCCATGACCAAGCAGGAGCTGGACGATATC CTTAAGTTCGGGACAGAGGAGCTCTTCAAGGATGACGTGGAGGGTATGATGTCCCAGGGCCAGAGGCCGA CCACGCCCATCCCTGATGTACAGTCCACCAAGGGCGGATCTCTGGCTGCCGGTGCAAAGAAAAAGCACGG CGGCACCCCGCCAGGCGACAACAAAGATGTAGAAGACAGCAGTGTGATCCACTACGACGACGCAGCCATA TCAAAGCTGCTGGACCGGAACCAGGACGCCACGGATGACACTGAGCTGCAGAACATGAACGAGTACCTGA GCTCCTTCAAAGTGGCCCAGTACGTGGTCCGTGAGGAAGATGGCGTGGAGGAGGTGGAGAGGGAGGTGAT CAAGCAGGAGGAGAATGTGGATCCTGACTACTGGGAGAAGCTTTTGCGCCATCACTACGAGCAGCAGCAG GAGGACTTGGCCCGCAACCTGGGCAAAGGCAAGCGCATCCGTAAGCAGGTCAACTACAACGACGCCTCCC AGGAGGACCAGGAGTGGCAGGATGAGCTCTCGGACAACCAGTCGGAATATTCCATCGGCTCTGAGGACGA GGACGAGGACTTCGAGGAGAGGCCAGAAGGGCAGAGCGGACGTCGACAATCCAGGAGGCAGCTGAAGAGT GACAGGGACAAGCCCCTGCCGCCTCTTCTGGCCCGAGTTGGGGGCAACATTGAGGTTCTGGGCTTCAATG CCAGACAGAGGAAGGCATTTTTGAATGCCATCATGCGTTGGGGCATGCCACCTCAGGATGCCTTCAACTC CCACTGGCTGGTGCGCGACCTTCGTGGGAAGAGTGAGAAGGAATTTAGAGCCTATGTATCACTCTTCATG CGCCACCTGTGTGAGCCCGGCGCAGACGGCGCGGAGACCTTTGCAGACGGCGTGCCCCGGGAGGGCCTGT CCAGGCAGCACGTACTCACGCGCATTGGGGTCATGTCGCTGGTTAGGAAGAAGGTGCAGGAGTTTGAACA TGTCAACGGCAAGTACAGCACCCCAGACCTGGTCCCCGAGGGGCCCGAGGGCAAGAAGCCAGGCGAGGTC ATCTCCTCGGACCCCAACACACCCGTGCCTGCCAGCCCTGCACAGCTCCCACCGGCTCCGCTGGGCCTGC CAGACAAAATGGAAGCCCAGCTGGGCTACACGGACGAGAAGGAGTCAGGCACACAGAAGCCAAAGAAGTC CCTGGAAATCCAGGCCCTGCCAACTGCCCTGGACAGAGTAGAGGCTGAAGACAAACATCAGAGCTCAGAC AGCAAGGACAGAGCTCGGGAGGAGAGGATGGAGGAGGTGGAGAAGGCTCAGGGCTCTCCGGAGCAGCCAC TGAAAGAGGAAACACTACCGGACAAGGAACCTGTCCCAGACAAGCTGGAGCTAAGCCTGAGTCACAGCAA CGATTTCAGGCCAGATGACCCCAAGGCCGAGGAGAAGGAGCCCACAGAGACCCAGCAAAACGGCGACAGA GAGGAAGACGAGGAGGGCAAGAAAGAAGACAAGAACGGGAAATTCAAGTTCATGTTCAACATTGCAGATG GTGGTTTCACAGAGCTGCACACGCTGTGGCAGAATGAGGAACGGGCAGCTGTGTCCTCGGGGAAAATCTA CGAAATCTGGCACCGTCGCCATGACTACTGGCTGCTGGCAGGCATTGTGACGCATGGCTATGCCCGCTGG CAGGACATTCAGAATGACCCGAGGTACATGATCCTAAATGAGCCTTTCAAGTCTGAGGTTCATAAGGGTA ACTACCTGGAGATGAAGAATAAGTTCTTGGCCCGTCGGTTCAAGCTGCTGGAGCAGGCGCTGGTGATCGA GGAGCAGCTCCGGAGGGCAGCGTACCTCAACATGACCCAGGATCCCAACCACCCAGCCATGGCGCTCAAT GCTCGCCTGGCAGAGGTGGAGTGCCTTGCCGAGAGCCACCAGCACCTATCCAAGGAGTCCCTTGCCGGGA ACAAGCCCGCCAACGCTGTCCTGCACAAGGTCCTGAACCAGCTGGAAGAGCTGCTGAGTGATATGAAAGC AGATGTGACACGCTTGCCCTCCATGTTATCACGCATCCCCCCGGTGGCCGCCCGTCTGCAGATGTCGGAA CGCAGCATCCTGAGTCGCCTGACCAACCGCGCCGGTGACCCTACCATCCAGCAGGGCGCTTTCGGCTCCT CTCAGATGTACAACAACAGCTTTGGGCCCAACTTCCGGGGCCCCGGACCGGGAGGGATTGTCAACTACAA CCAGATGCCCCTGGGGCCCTATGTGACTGACATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271226
Insert Size	5847 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271226.2, NP_001258155.1
RefSeq Size	6528 bp
RefSeq ORF	5847 bp
Locus ID	691589

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