Scn5a (NM_001160162) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nav1.5; SCAL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217715 representing NM_001160162 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACCTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA TCGAGAAGCGAATGGCTGAAAAGCAAGCCCGAGGAGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCT GCAGGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGC AACCCACCCCGAGAGCTCATCGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCT TCATCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTATGTCCTCAGCCC CTTCCACCCCGTGCGCCGAGCGGCCGTGAAGATCCTGGTACACTCGCTCTTTAGCATGCTCATCATGTGC ACCATCCTGACCAACTGCGTGTTCATGGCCCAGCACGACCCTCCGCCTTGGACCAAATATGTTGAGTACA CCTTCACTGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCATT CACCTTCCTTCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCATACACAACTGAATTT GTGGACCTGGGCAATGTCTCAGCCTTACGCACCTTCCGAGTCCTCCGGGCCCTGAAAACTATATCGGTCA TTTCAGGCCTGAAGACCATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAACTGGCCGATGTGATGGTCCT CACTGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAG TGTGTGCGTAACTTCACCGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGACGGCCTAGTCTGGAACT CCCTGGACGTCTACCTCAATGACCCAGCCAATTACCTGCTCAAGAATGGCACCACGGATGTGTTACTATG TGGGAACAGCTCTGATGCCGGGACATGCCCTGAGGGCTATCGGTGCCTGAAGGCAGGTGAGAACCCAGAC CACGGTTACACCAGCTTCGACTCCTTCGCCTGGGCCTTCCTTGCACTCTTCCGCCTGATGACACAGGACT GCTGGGAACGCCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTCGT CATCTTTCTGGGCTCCTTCTACCTGGTGAACTTGATCCTGGCTGTGGTGGCCATGGCCTACGAGGAGCAA AACCAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGCTTCCAGGAGGCCATGGAGATGCTCAAGA AGGAACACGAGGCTCTCACCATCAGGGGTGTGGATACCGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTT GGCCCCAGTAACCAACCATGAGAGAAAGAGCAAAAGGAGGAAACGACTATCTTCAGGGACAGAGGATGGT GGGGATGACAGGCTCCCCAAGTCGGACTCAGAAGATGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCC ATGGGCTCAGCCGGACATCCATGAGGCCCCGCTCGAGCCGAGGGAGCATTTTCACGTTCCGAAGACGGGA CCAAGGCTCTGAGGCGGACTTCGCAGATGACGAGAACAGCACTGCGGGGGAGAGCGAGAGCCACCGCACA TCGCTGCTGGTACCCTGGCCCCTGCGCCATCCCAGCGCCCAAGGACAGCCCGGCCCTGGAGCCTCAGCTC CCGGTTACGTTCTCAATGGCAAAAGGAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGC AGGTGACGCAGAGGCCACCTCCCCAGGGAGCTACCTTCTCCGCCCTATGGTGCTGGACCGACCCCCAGAC ACGACCACTCCGTCAGAGGAGCCCGGTGGGCCCCAGATGCTGACACCTCAGGCTCCGTGTGCAGATGGTT TTGAGGAGCCCGGAGCACGGCAACGGGCACTCAGCGCTGTCAGTGTCCTCACCAGTGCCCTGGAAGAGTT GGAGGAGTCCCATCGGAAGTGTCCACCATGCTGGAACCGCTTTGCCCAGCACTACCTCATCTGGGAGTGC TGTCCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTTGTGGTCATGGACCCATTTGCCGACCTCACTA TCACCATGTGCATCGTGCTCAATACGCTCTTCATGGCTCTGGAGCATTACAACATGACGGCAGAGTTTGA GGAGATGCTGCAGGTCGGAAACCTGGTCTTCACGGGAATCTTCACAGCGGAGATGACCTTCAAGATCATC GCCCTTGACCCCTACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGTC TCATGGAGCTGGGGCTGTCCCGCATGGGCAACTTGTCTGTGCTACGTTCCTTCCGTCTGCTGCGGGTCTT CAAGCTGGCCAAGTCCTGGCCCACCCTGAACACGCTCATCAAGATCATCGGGAACTCCGTGGGCGCCCTG GGGAACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTCTTCGGCA AGAACTACTCGGAGCTGAGGCACCGCATCAGCGACTCCGGCCTGCTGCCCCGCTGGCACATGATGGACTT TTTCCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGGGAGTGGATCGAGACCATGTGGGACTGCATG GAGGTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTCGTCATGGTCATTGGCAACCTTGTGGTCC TGAATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGG GGAGATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAGAGGGGCCTGCGCTTTGTCAAGCGGACCACC TGGGACTTCTGCTGCGGGATCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGC TGCCCAGCTGTATCACCGCCCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGTGCCCCCAGCCCGCAA GGAAACACGATTCGAGGAGGACAAGCGACCCGGCCAGGGCACCCCTGGGGATTCGGAGCCTGTGTGTGTG CCCATCGCCGTGGCTGAGTCAGACACTGAAGACCAGGAAGAGGATGAAGAGAACAGCCTTGGCACAGAGG AAGAGTCCAGCAAACAGACCCCTGAGGACAGTTACTCCGAGGGCAGCACAGCTGACATGACCAACACCGC CGACCTCCTGGAGCAAATCCCAGACCTTGGTGAGGACGTCAAGGACCCAGAGGACTGCTTTACTGAAGGC TGCGTCCGACGCTGTCCCTGCTGCATGGTAGACACAACCCAGTCCCCAGGGAAGGTCTGGTGGCGATTGC GCAAGACCTGCTACCGCATCGTGGAGCACAGCTGGTTCGAGACTTTCATCATCTTCATGATCCTGCTCAG CAGTGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGACCATCAAGGTTCTGCTGGAGTAC GCGGACAAGATGTTCACCTACGTCTTTGTGTTGGAGATGCTGCTCAAGTGGGTGGCCTACGGCTTCAAGA AGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTGATTGTGGACGTCTCGCTGGTCAGCCTCGTGGC AAACACCTTAGGCTTCGCCGAAATGGGTCCCATCAAGTCACTGAGGACACTGCGTGCACTTCGACCCCTG AGGGCCTTGTCGAGATTTGAGGGCATGCGGGTGGTGGTCAATGCGCTGGTGGGCGCCATCCCCTCCATCA TGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCATGGGCGTGAACCTCTTCGCCGG GAAGTTCGGTAGGTGCATCAACCAGACAGAAGGGGACCTGCCTCTGAACTACACCATCGTGAACAACAAG AGTGAGTGCGAGTCCTTCAACGTGACCGGAGAGTTGTACTGGACCAAGGTGAAGGTCAACTTTGACAACG TGGGAGCCGGGTACCTGGCCCTCCTGCAGGTGGCGACATTTAAAGGCTGGATGGACATCATGTATGCGGC TGTGGACTCCAGAGGGTATGAGGAGCAGCCGCAGTGGGAAGACAACCTCTACATGTACATCTACTTTGTC GTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCATCGGTGTCATCATTGACAACTTCAACC AGCAGAAGAAAAAGTTAGGGGGCCAGGATATCTTCATGACGGAGGAGCAGAAGAAGTACTACAATGCCAT GAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCCTTGAACAAGTACCAGGGTTTCATA TTCGACATTGTGACCAAGCAGGCCTTCGATGTCACCATCATGTTCCTCATCTGTTTGAACATGGTGACCA TGATGGTGGAGACAGATGACCAGAGCCCTGAGAAGGTCAACATCTTGGCCAAGATCAACCTGCTCTTCGT GGCCATCTTCACAGGCGAGTGTATTGTCAAGATGGCTGCCCTGCGCCACTATTACTTCACCAACAGCTGG AACATCTTCGACTTTGTGGTGGTCATCCTCTCCATTGTTGGCACTGTCCTCTCCGACATCATCCAGAAGT ACTTCTTCTCCCCGACACTCTTCCGGGTCATCCGTCTGGCCAGGATCGGCCGCATCCTCAGGCTGATCCG CGGAGCCAAGGGGATTCGCACGCTGCTCTTCGCCCTCATGATGTCCCTGCCCGCCCTCTTCAACATCGGC CTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCCAACTTCGCTTACGTCAAGTGGG AGGCCGGCATCGATGACATGTTCAACTTCCAGACCTTCGCCAACAGCATGCTGTGCCTGTTCCAGATCAC CACATCAGCCGGCTGGGACGGCCTCCTCAGCCCCATCCTCAACACGGGGCCTCCCTACTGCGACCCCAAC CTGCCCAACAGCAACGGCTCCCGGGGGAACTGTGGGAGCCCGGCGGTGGGCATCCTCTTCTTCACCACCT ACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATCGCCATCATCCTCGAGAACTTCAGCGTGGC CACCGAGGAGAGCACAGAGCCCCTGAGCGAGGACGACTTCGACATGTTCTATGAGATCTGGGAGAAGTTC GACCCGGAGGCCACCCAGTTCATTGAGTATCTGGCCCTGTCCGACTTTGCAGATGCCTTGTCTGAGCCGC TCCGCATCGCCAAACCCAACCAGATAAGCCTCATCAACATGGATCTGCCCATGGTGAGCGGAGACCGTAT CCACTGTATGGACATACTGTTCGCTTTCACCAAGAGGGTGCTCGGCGAGTCTGGGGAGATGGATGCCCTG AAGATCCAGATGGAGGAGAAGTTCATGGCGGCCAACCCTTCCAAGATCTCCTACGAGCCCATCACCACCA CCCTGAGGAGAAAGCACGAGGAGGTGTCGGCCACGGTCATCCAGCGTGCCTTCCGGAGGCACCTGCTGCA GCGCTCGGTGAAGCATGCCTCCTTTCTCTTCCGCCAGCAAGCGGGCGGCAGTGGCCTCTCCGACGAGGAT GCCCCTGAGCGGGAGGGCCTCATCGCCTACATGATGAATGGGAACTTCTCTCGGCGCAGTGCTCCGCTCT CCAGCTCCTCCATCTCCTCCACGTCCTTCCCCCCGTCCTACGACAGCGTCACGAGAGCCACCAGTGATAA CCTCCCGGTGCGTGCGTCTGACTATAGCCGCAGCGAAGATCTTGCAGACTTCCCTCCATCTCCAGATAGG GACCGAGAGTCTATCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001160162
Insert Size	5901 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001160162.1, NP_001153634.1
RefSeq Size	8309 bp
RefSeq ORF	5901 bp
Locus ID	25665
UniProt ID	P15389
Gene Summary	acts as a tetrodotoxin-resistant voltage gated sodium channel; may play a role in cardiac function [RGD, Feb 2006] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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