Numa1 (NM_001100691) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217736 representing NM_001100691 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCATGCCACCCGCGCATCTACTCTCCTCTCTTGGGTGAACAGTCTGCATGTTGCTGACCCAG TGGAGACAGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATTATTAACAGCATCCATGACACTAA AGAGGGGCAACAAATCCTACAGCAGCCACTGCCGGAAAGACTAGACTTTGTTTGCAGTTTTCTACAGAAA AATCGGAAGCATCCTTCATCTACACAATGCCTGGTATCTGTGCAGAAAGTGATGGAGGGCTCCGAGATGG AACTGGCCAAGATGGTAATGCTATTTTTATATCACTCCACCATGAGCTCCAGAAACCCCCGGGACTGGGA GCAGTTTGAATATGGGGTTCAGGCTGAGTTGGCTGTCATCCTGAAGTTTATGCTGGATCACGAGGATTCA CCTAACCTTACTGAGGACTTGGAGAATTTCTTGGAGAAAGTTCCTTCCACCTACCCCAGCACCCTCTCTG AAGAGCTTTCTCCACCCAGCCACCAGGCCAAGAGGAAGATTCGCTTCCTAGAAATACAGAGGATTGCTTC ATCTTCCAGTGAGAACAACTTCCTCTCAGGGTCTCCATCCTCCCCTGTGGGCGACATCCTGCAGACCCCA CAGTTCCAGATGAGGCGACTGAAGAAGCAGCTGGCAGATGAGAGGAACAACAGGGATGAGCTGGAGCTAG AGCTGTCTGAGAGCCTCAAGCTCCTCACCGAGAAGGACGCTCAGATCGCCATGATGCAGCAGCGCATCGA CCACCTGGCTCTGCTGAATGAGAAGCAGGCAGCCAGCCCCCAGGAGCCCAGCGAACTTGAGGAGCTCCGT GGCAAGAATGAAAGCCTCACTGTGCGTCTGCATGAGACTTTGAAGCAGTGTCAGAACCTGAAGACCGAGA AGAACCAGATGGATCGAAAGATTAGCCAGCTTTCTGAGGAGAACGGGGACCTTTCCTTTAAGGTGCGAGA GTTTGCAAACCACTTGCAACAACTGCAGGGTGCGTTCAACGACCTGATAGAGGAGCACAGCAAGGCTTCT CAGGAGTGGGCAGAGAAGCAGGCTCATCTGGAAAGTGAGCTCAGCACAGCCCTCCAGGATAAGAAATGTC TGGAAGAGAAGAGTGAGATCCTTCAGGAGAAGATTTCCCAGCTGGAGGATCGAGCAGCTCAGCTGCAGGG GAGCCCTGCCCCGGAGAAGGGTGAGGTGCTGGGTGACGCCTTGCAGCTGGATACCCTGAAACAAGAGGCA GCCAAACTTGCCACAGATAACACAGAGCTCCAAGCCCGCGTAGAGACTCTGGAGTGTGAGCGTGGTAAAC AGGAAGCCCAGCTGCTTGCTGAGCGGGGCCACTTTGAAGAAGAGAAGCGGCAGCTGGCCAGCTTGGTTGC TGACCTGCAGAGCTCCGTCTCTAACCTCAGCCAGGCCAAAGAGGAGCTGCAGCAAGCCTCCCAGGCTCAG GGGGCTCAGCTCACTGCCCAGCTGGCCTCTCTGACAGCACTGAATGCCACCCTGCAGCAGCAAGATCAAG AGCTGACCAGCCTGAAAGAACAGGCCAAAAAGGAACAGGCTCAGATGTTACAGACCTTGCAAGAGCAAGA ACAGGCTGCCCAGGGCCTCCGCCAGCAGGTGGAGCAGCTGAGCAGCAGCCTGAAGCTGAAGGAGCAGCAA TTGGAAGAGGCAGCCAAGGAGCAGGAGGCAGCCAGGCAGGATCACGCCCAGCAACTGGCCACCATTGTTG AGGCTCGAGAGGCCTCTGTAAGGGAGCGAGATGCTGCTCGCCAGCAGCTAGAAACATTGGAGAAGGAGAA GGATGCCAAGCTGGAGAGCCTGCAGCAGCAACTTCAGGCTTCTAATGAAGCCAGGGACACTGCGCAGACC TCGGTCACACAGGCCCAGCGGGAAAAGGCAGAGCTGAGCCAAAAGATAGGAGAACTCCATGCCTGCATTG AAGCTGCCCACCAGGAACAGCGTCAGGCCCAAGCTCACGTGACAGAGCTAGAGGCCCAGCTGAAGGCTGA GCAGCAAAAAGCAACTGAGAGAGAAAAGGTGGTGCAGGAGAAGGTCCAGCTCCAGGAGCAGCTCCAGGCC CTTGAGGAGACTTTGAAGATTGTCAGAGGTAGCCTTGAAGAAGAGAAATGCAGGGCTGCAGATGCCCTGA AGGAGCAGCAGCGCCACGCCACTGAGATGGAGGCAGAGACTCGACACCTGATGGAGCAGCGGGAGCAGGA GCAGAAGGAGTTAGAGCAGGAGAAGGCTGAGCGAAAGGGGCTGGAGGCCCGGCTACAGCAGCTTGAAGAG GCTCATCAGGCCGAGACTGAAGCCTTGCGACATGAGCTAGCAGGGGCCACAGCTGCTCAGCACGGAGCTG AGAGTGAGCGTGAGCAGCTCCTCAGGGAGGTTGAGAGCTGGCAGAAGCGGGTTGAAGCCAGACAGCAAGA GGAGGCAAGGTATGGTGCCATGTTCCAGGAACAGCTGATGGCCTTGAAAGGGGAGCATGGAAAGATTGGC CAAGAAGAACAGAAGGAGGCAGGAGAGATCCACGGTGAAGGCCAGACAGGCCAGCAGCAGAGTCAGCTAG CTCAGCTTCATGCCTGTTTGGCAAAAGCCCTTCAGCAGGTCCAGGAGAAAGAAGCGAGAGCACAGAAGCT GCTAGATGATCTCTCGGCTCTGCGGGAGAAGATGGCTGCCACTAACAAGGAGGTGGCCTGCCTGAAGGCC TTGGTGCTCAAGGCAGGTGAGCAGCAGGCAGCAGCCTCACATGAGCTCAAAGAGCCCCCGAGGGCTGGGA ACCAAGAGTCTGACTGGGAGGAAGAGCAGGCAAGGCCGTTGGGTAGTACACAGGCAGCACTGAAGGCTGT GCAGCGTGAAGCTGAGCAAATGGGCGGAGAGCTGGAGAGACTGCGGGCAGCACTGATGCAGAGCCAGGGG CAGCAGCAAGAGGTTCGTGGGCAGCAGGAGAGGGAGGTAGCACGGCTGACCCAGGAGCGGGGCCAGGCCC AAGCTGACCTGGCTCAGGAGAAGGCAGCCAAGGCAGAACTTGAGATGCGGCTGCAGAACACCCTCAATGA GCAGCGTGTGGAGTTTGCTGCCTTACAAGAAGCACTGACCCATGCCATGACAGAAAAGGAAGGGAAAGAC CAAGAGCTTGCCAAACTTCGTGAGCAAGAGGCAGCTCAGATATCAGAGCTGAAGGCGCTTCAGCAGACTT TGGAAGAGCTGAAGAAGAAAGAGAAAGAGCACCCCACAGGGGGTGCCAGGGGAGAAGACGCTTCAGGAGA TGGCCCAGGATCCCAGCTGCACACACCTGGGAAGACAGAGGCACCAGGCCCTGAGGTGGAGGCTCTTCGG GCAGAGATCAGTAAGCTAGAGCGGCAGTGGCAGCAGCAGCAGCAACAGGTTGAAGGCCTGACACACAGCC TGGAGTCCGAGCGTGCTTGCCGGGCTGAGCAAGACAAAGCCCTGGAGACACTGCAGGGCCAGTTAGAGGA GAAGGCTCGAGAGCTGGGGCATAATCAGGCTGCCTCAGCCTCAGCTCAAAGAGAGTTGCAAGCCCTCCGT GCCAAAGCCCAGGACCACAGCAAAGCTGAGGAAGAGTGGAAGGCCCAGGTAGCCCGTGGCCAGCAGGAAG CTGAGAGAAAGAGCAGTCTTATCAGCAGCTTGGAAGAGGAGGTATCCATCCTGAATCGCCAAGTCCTAGA GAAGGAGGGAGAAAGCAAGGAGTTGAAGCGCCTGGTTGTAGCTGAGTCAGAGAAGAGCCAGAAGCTGGAA GAGCGGCTGCGCCTGCTTCAGGTAGAAACAGCCAGCAGCAGTGCCAGGGCAGCAGAACGCAGCTCAGCTC TACGGGAGGAGGTACAGAGCCTCCGAGAAGAGGTAGAGAAGCAGCGTGTAGTTTCTGAGAACTTGAGGCA GGAGCTGGCCTCACAAGCGGAGCGAGCTGAGGAATCGGGTCAAGAATTGAAGGCCTGGCAGGAGAAGTTT TTCCAGAAGGAGCAAGCGCTCTCTGCCCTGCAGCTGGAGCACACCAGCACACAGGCCCTGGTGAGTGAGC TGCTGCCTGCCAAGCACCTTTGTCAGCAGCTGCAAGCTGAGCAGGCAGCTGCCGAGAAGCGCTTCCGGGA GGAGATAGAGCAGAGCAAGCAGGCAGCTGGCGGGCTGCAGGCAGAGCTAATGCGGGCTCAACGGGAGCTT GGAGAGCTAGGATCTTTGCGGCAGAAGATTGTTGAGCAGGAGCGAGCAGCCCAGCAGCTGCGGGCAGAAA AGGCCAGCTATGCAGAGCAGTTGAGCATGCTGAAGAAGGCCCATGGCCTGCTGGCCGAGGAGAACCGGGG GCTGGGAGAACGGGCCAACCTGGGCCGGCAGTTTCTAGAAGTAGAGCTCGATCAAGCCCGGGAGAAGTAT GTCCAGGAGCTGGCCGCTGTGCGCACTGATGCCGAGACCCACCTGGCTGAAATGCGGCAAGAAGCTCAGA GCACTAGCCGGGAGCTGGAGGTGATGACCGCCAAGTACGAAAGTGCCAAGGTGAAGGTCCTAGAGGAGAG GCAGAGGTTCCAAGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTGAATAAGAAGCTGACAGAG AGTGAACAAGCCAGCAGGGTGCAGCAGCAGAAGCTGAAGGCTTTCCAGGCCCAGGGAGGTGAAAGCCAGC AGGAGGTCCAGTGCCTCCAGACGCAGCTGAGTGAGCTGCAAGCCCAGCTGAGCCAGAAGGAGCAGGCAGC TGAGCACTACAAGCTACAGATGGAGAAAGCCAAGACCCATTACGATGCCAAGAAGCAGCAGAACCAAGAG CTGCGGGAACAGCTTCAAGACCTGGAGCAGCTGCAGAAGGACAACAAGGAGCTGCGGTCTGAGACTGAAC GTCTGGGCCGTGAGCTGCAGCAGGCAGGGCTGAAGACCAAGGAAGCTGAACAGGCTTGCCGGCACCTCAC AGCCCAGGTGCGCAGCCTGGAGGCCCAGGTTGCCCACGCAGACCAGCAGCTTCGAGACCTGGGCAAATTC CAGGTGGCAACTGATGCCTTAAAGAGCCGTGAGCCTCAAGTGAAACCCCAGCTGGACCTAAGTATTGACA GCCTGGATCTGAGCTTGGAGGAGGGAACCCCGTGCAGTGTCGCTAGCAAGCTGCCTCGTACCCAGCCAGA TGGTACCAGTGTCCCAGGAGAGCCAGCCTCACCCATCTCCCAGCGCTTGCCCCCCAAAGTGGAATCCCTG GAGAGTCTCTACTTCACCCCCATTCCTGCTCGGGGTCAGGCCCCCTTGGAGACCAGCCTGGACTCTCTGG GGGATGCCTTCCCTGACTCCGGCCGTAAGACCCGCTCTGCTCGCCGGCGTACCACACAGATCATCAACAT TACCATGACCAAGAAACTAGAACTGGAGGAGCCGGACAGCGCCAACTCGTCCTTCTACAGTACGCAGTCT GCCCCTGCTTCCCAGGCCAACTTGCGAGCCACTTCCTCCACTCAGTCTCTGGCCCGCTTGGGCTCTCCTG ATGACAGCAACTCCGCGCTGCTCAGCCTGCCTGGCTACCGGCCTACCACCCGAAGCTCTGCTCGGCGCTC CCAGACCAGGACATCCAGTGGGGCCCCCCACGGAAGGAATAGCTTCTACATGGGGACTTGCCAGGACGAG CCCGAGCAGGTGGATGACTGGAACCGCATCGCGGAGTTGCAGCAGCGCAACAGAGTATGCCCTCCGCACC TGAAGACCTGCTACCCTCTGGAGTCCAGGCCTGCCCTGAGCCTGGCCACCATCACTGACGAGGAGATGAA AACCGGAGACCCCCGGGAAACCTTGCGCAGAGCCAGCATGCAGCCAGCTCAGATCGCTGAGGGCGCTGGC ATCACTACCAGGCAGCAGCGTAAACGGGTTTCCTCGGAGACCCACCAGGGCCCCGGTACTCCTGAGTCCA AGAAAGCTACGAGCTGCTTTCCACGCCCCATGACTCCCAGGGACCGGCATGAAGGACGGAAGCAGAACAG CACTGCTGATGCCCAGAAGAAAGCAGCTCCAGTTCTCAAGCAGTCGGACCGGCGTCAGTCAATGGCTTTC AGCATCCTTAACACACCCAAGAAGCTGGGAAATAGCCTTCTACGGCGAGGAACTTCAAAGAAGACCCCAG CAAAGGTTTCCCCCAATCCTCGCAGTGGAACCCGCCGCTCTCCACGCATCGCCACCACCACGGCTGGTGC TGCTACTGCTGCCACCACTCCTCGGGCCAAGGGCAAGGCAAAGCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100691
Insert Size	6279 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100691.1, NP_001094161.1
RefSeq Size	7793 bp
RefSeq ORF	6279 bp
Locus ID	308870

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