Numa1 (NM_001100691) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217736 representing NM_001100691 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCATGCCACCCGCGCATCTACTCTCCTCTCTTGGGTGAACAGTCTGCATGTTGCTGACCCAG TGGAGACAGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATTATTAACAGCATCCATGACACTAA AGAGGGGCAACAAATCCTACAGCAGCCACTGCCGGAAAGACTAGACTTTGTTTGCAGTTTTCTACAGAAA AATCGGAAGCATCCTTCATCTACACAATGCCTGGTATCTGTGCAGAAAGTGATGGAGGGCTCCGAGATGG AACTGGCCAAGATGGTAATGCTATTTTTATATCACTCCACCATGAGCTCCAGAAACCCCCGGGACTGGGA GCAGTTTGAATATGGGGTTCAGGCTGAGTTGGCTGTCATCCTGAAGTTTATGCTGGATCACGAGGATTCA CCTAACCTTACTGAGGACTTGGAGAATTTCTTGGAGAAAGTTCCTTCCACCTACCCCAGCACCCTCTCTG AAGAGCTTTCTCCACCCAGCCACCAGGCCAAGAGGAAGATTCGCTTCCTAGAAATACAGAGGATTGCTTC ATCTTCCAGTGAGAACAACTTCCTCTCAGGGTCTCCATCCTCCCCTGTGGGCGACATCCTGCAGACCCCA CAGTTCCAGATGAGGCGACTGAAGAAGCAGCTGGCAGATGAGAGGAACAACAGGGATGAGCTGGAGCTAG AGCTGTCTGAGAGCCTCAAGCTCCTCACCGAGAAGGACGCTCAGATCGCCATGATGCAGCAGCGCATCGA CCACCTGGCTCTGCTGAATGAGAAGCAGGCAGCCAGCCCCCAGGAGCCCAGCGAACTTGAGGAGCTCCGT GGCAAGAATGAAAGCCTCACTGTGCGTCTGCATGAGACTTTGAAGCAGTGTCAGAACCTGAAGACCGAGA AGAACCAGATGGATCGAAAGATTAGCCAGCTTTCTGAGGAGAACGGGGACCTTTCCTTTAAGGTGCGAGA GTTTGCAAACCACTTGCAACAACTGCAGGGTGCGTTCAACGACCTGATAGAGGAGCACAGCAAGGCTTCT CAGGAGTGGGCAGAGAAGCAGGCTCATCTGGAAAGTGAGCTCAGCACAGCCCTCCAGGATAAGAAATGTC TGGAAGAGAAGAGTGAGATCCTTCAGGAGAAGATTTCCCAGCTGGAGGATCGAGCAGCTCAGCTGCAGGG GAGCCCTGCCCCGGAGAAGGGTGAGGTGCTGGGTGACGCCTTGCAGCTGGATACCCTGAAACAAGAGGCA GCCAAACTTGCCACAGATAACACAGAGCTCCAAGCCCGCGTAGAGACTCTGGAGTGTGAGCGTGGTAAAC AGGAAGCCCAGCTGCTTGCTGAGCGGGGCCACTTTGAAGAAGAGAAGCGGCAGCTGGCCAGCTTGGTTGC TGACCTGCAGAGCTCCGTCTCTAACCTCAGCCAGGCCAAAGAGGAGCTGCAGCAAGCCTCCCAGGCTCAG GGGGCTCAGCTCACTGCCCAGCTGGCCTCTCTGACAGCACTGAATGCCACCCTGCAGCAGCAAGATCAAG AGCTGACCAGCCTGAAAGAACAGGCCAAAAAGGAACAGGCTCAGATGTTACAGACCTTGCAAGAGCAAGA ACAGGCTGCCCAGGGCCTCCGCCAGCAGGTGGAGCAGCTGAGCAGCAGCCTGAAGCTGAAGGAGCAGCAA TTGGAAGAGGCAGCCAAGGAGCAGGAGGCAGCCAGGCAGGATCACGCCCAGCAACTGGCCACCATTGTTG AGGCTCGAGAGGCCTCTGTAAGGGAGCGAGATGCTGCTCGCCAGCAGCTAGAAACATTGGAGAAGGAGAA GGATGCCAAGCTGGAGAGCCTGCAGCAGCAACTTCAGGCTTCTAATGAAGCCAGGGACACTGCGCAGACC TCGGTCACACAGGCCCAGCGGGAAAAGGCAGAGCTGAGCCAAAAGATAGGAGAACTCCATGCCTGCATTG AAGCTGCCCACCAGGAACAGCGTCAGGCCCAAGCTCACGTGACAGAGCTAGAGGCCCAGCTGAAGGCTGA GCAGCAAAAAGCAACTGAGAGAGAAAAGGTGGTGCAGGAGAAGGTCCAGCTCCAGGAGCAGCTCCAGGCC CTTGAGGAGACTTTGAAGATTGTCAGAGGTAGCCTTGAAGAAGAGAAATGCAGGGCTGCAGATGCCCTGA AGGAGCAGCAGCGCCACGCCACTGAGATGGAGGCAGAGACTCGACACCTGATGGAGCAGCGGGAGCAGGA GCAGAAGGAGTTAGAGCAGGAGAAGGCTGAGCGAAAGGGGCTGGAGGCCCGGCTACAGCAGCTTGAAGAG GCTCATCAGGCCGAGACTGAAGCCTTGCGACATGAGCTAGCAGGGGCCACAGCTGCTCAGCACGGAGCTG AGAGTGAGCGTGAGCAGCTCCTCAGGGAGGTTGAGAGCTGGCAGAAGCGGGTTGAAGCCAGACAGCAAGA GGAGGCAAGGTATGGTGCCATGTTCCAGGAACAGCTGATGGCCTTGAAAGGGGAGCATGGAAAGATTGGC CAAGAAGAACAGAAGGAGGCAGGAGAGATCCACGGTGAAGGCCAGACAGGCCAGCAGCAGAGTCAGCTAG CTCAGCTTCATGCCTGTTTGGCAAAAGCCCTTCAGCAGGTCCAGGAGAAAGAAGCGAGAGCACAGAAGCT GCTAGATGATCTCTCGGCTCTGCGGGAGAAGATGGCTGCCACTAACAAGGAGGTGGCCTGCCTGAAGGCC TTGGTGCTCAAGGCAGGTGAGCAGCAGGCAGCAGCCTCACATGAGCTCAAAGAGCCCCCGAGGGCTGGGA ACCAAGAGTCTGACTGGGAGGAAGAGCAGGCAAGGCCGTTGGGTAGTACACAGGCAGCACTGAAGGCTGT GCAGCGTGAAGCTGAGCAAATGGGCGGAGAGCTGGAGAGACTGCGGGCAGCACTGATGCAGAGCCAGGGG CAGCAGCAAGAGGTTCGTGGGCAGCAGGAGAGGGAGGTAGCACGGCTGACCCAGGAGCGGGGCCAGGCCC AAGCTGACCTGGCTCAGGAGAAGGCAGCCAAGGCAGAACTTGAGATGCGGCTGCAGAACACCCTCAATGA GCAGCGTGTGGAGTTTGCTGCCTTACAAGAAGCACTGACCCATGCCATGACAGAAAAGGAAGGGAAAGAC CAAGAGCTTGCCAAACTTCGTGAGCAAGAGGCAGCTCAGATATCAGAGCTGAAGGCGCTTCAGCAGACTT TGGAAGAGCTGAAGAAGAAAGAGAAAGAGCACCCCACAGGGGGTGCCAGGGGAGAAGACGCTTCAGGAGA TGGCCCAGGATCCCAGCTGCACACACCTGGGAAGACAGAGGCACCAGGCCCTGAGGTGGAGGCTCTTCGG GCAGAGATCAGTAAGCTAGAGCGGCAGTGGCAGCAGCAGCAGCAACAGGTTGAAGGCCTGACACACAGCC TGGAGTCCGAGCGTGCTTGCCGGGCTGAGCAAGACAAAGCCCTGGAGACACTGCAGGGCCAGTTAGAGGA GAAGGCTCGAGAGCTGGGGCATAATCAGGCTGCCTCAGCCTCAGCTCAAAGAGAGTTGCAAGCCCTCCGT GCCAAAGCCCAGGACCACAGCAAAGCTGAGGAAGAGTGGAAGGCCCAGGTAGCCCGTGGCCAGCAGGAAG CTGAGAGAAAGAGCAGTCTTATCAGCAGCTTGGAAGAGGAGGTATCCATCCTGAATCGCCAAGTCCTAGA GAAGGAGGGAGAAAGCAAGGAGTTGAAGCGCCTGGTTGTAGCTGAGTCAGAGAAGAGCCAGAAGCTGGAA GAGCGGCTGCGCCTGCTTCAGGTAGAAACAGCCAGCAGCAGTGCCAGGGCAGCAGAACGCAGCTCAGCTC TACGGGAGGAGGTACAGAGCCTCCGAGAAGAGGTAGAGAAGCAGCGTGTAGTTTCTGAGAACTTGAGGCA GGAGCTGGCCTCACAAGCGGAGCGAGCTGAGGAATCGGGTCAAGAATTGAAGGCCTGGCAGGAGAAGTTT TTCCAGAAGGAGCAAGCGCTCTCTGCCCTGCAGCTGGAGCACACCAGCACACAGGCCCTGGTGAGTGAGC TGCTGCCTGCCAAGCACCTTTGTCAGCAGCTGCAAGCTGAGCAGGCAGCTGCCGAGAAGCGCTTCCGGGA GGAGATAGAGCAGAGCAAGCAGGCAGCTGGCGGGCTGCAGGCAGAGCTAATGCGGGCTCAACGGGAGCTT GGAGAGCTAGGATCTTTGCGGCAGAAGATTGTTGAGCAGGAGCGAGCAGCCCAGCAGCTGCGGGCAGAAA AGGCCAGCTATGCAGAGCAGTTGAGCATGCTGAAGAAGGCCCATGGCCTGCTGGCCGAGGAGAACCGGGG GCTGGGAGAACGGGCCAACCTGGGCCGGCAGTTTCTAGAAGTAGAGCTCGATCAAGCCCGGGAGAAGTAT GTCCAGGAGCTGGCCGCTGTGCGCACTGATGCCGAGACCCACCTGGCTGAAATGCGGCAAGAAGCTCAGA GCACTAGCCGGGAGCTGGAGGTGATGACCGCCAAGTACGAAAGTGCCAAGGTGAAGGTCCTAGAGGAGAG GCAGAGGTTCCAAGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAAGAACTGAATAAGAAGCTGACAGAG AGTGAACAAGCCAGCAGGGTGCAGCAGCAGAAGCTGAAGGCTTTCCAGGCCCAGGGAGGTGAAAGCCAGC AGGAGGTCCAGTGCCTCCAGACGCAGCTGAGTGAGCTGCAAGCCCAGCTGAGCCAGAAGGAGCAGGCAGC TGAGCACTACAAGCTACAGATGGAGAAAGCCAAGACCCATTACGATGCCAAGAAGCAGCAGAACCAAGAG CTGCGGGAACAGCTTCAAGACCTGGAGCAGCTGCAGAAGGACAACAAGGAGCTGCGGTCTGAGACTGAAC GTCTGGGCCGTGAGCTGCAGCAGGCAGGGCTGAAGACCAAGGAAGCTGAACAGGCTTGCCGGCACCTCAC AGCCCAGGTGCGCAGCCTGGAGGCCCAGGTTGCCCACGCAGACCAGCAGCTTCGAGACCTGGGCAAATTC CAGGTGGCAACTGATGCCTTAAAGAGCCGTGAGCCTCAAGTGAAACCCCAGCTGGACCTAAGTATTGACA GCCTGGATCTGAGCTTGGAGGAGGGAACCCCGTGCAGTGTCGCTAGCAAGCTGCCTCGTACCCAGCCAGA TGGTACCAGTGTCCCAGGAGAGCCAGCCTCACCCATCTCCCAGCGCTTGCCCCCCAAAGTGGAATCCCTG GAGAGTCTCTACTTCACCCCCATTCCTGCTCGGGGTCAGGCCCCCTTGGAGACCAGCCTGGACTCTCTGG GGGATGCCTTCCCTGACTCCGGCCGTAAGACCCGCTCTGCTCGCCGGCGTACCACACAGATCATCAACAT TACCATGACCAAGAAACTAGAACTGGAGGAGCCGGACAGCGCCAACTCGTCCTTCTACAGTACGCAGTCT GCCCCTGCTTCCCAGGCCAACTTGCGAGCCACTTCCTCCACTCAGTCTCTGGCCCGCTTGGGCTCTCCTG ATGACAGCAACTCCGCGCTGCTCAGCCTGCCTGGCTACCGGCCTACCACCCGAAGCTCTGCTCGGCGCTC CCAGACCAGGACATCCAGTGGGGCCCCCCACGGAAGGAATAGCTTCTACATGGGGACTTGCCAGGACGAG CCCGAGCAGGTGGATGACTGGAACCGCATCGCGGAGTTGCAGCAGCGCAACAGAGTATGCCCTCCGCACC TGAAGACCTGCTACCCTCTGGAGTCCAGGCCTGCCCTGAGCCTGGCCACCATCACTGACGAGGAGATGAA AACCGGAGACCCCCGGGAAACCTTGCGCAGAGCCAGCATGCAGCCAGCTCAGATCGCTGAGGGCGCTGGC ATCACTACCAGGCAGCAGCGTAAACGGGTTTCCTCGGAGACCCACCAGGGCCCCGGTACTCCTGAGTCCA AGAAAGCTACGAGCTGCTTTCCACGCCCCATGACTCCCAGGGACCGGCATGAAGGACGGAAGCAGAACAG CACTGCTGATGCCCAGAAGAAAGCAGCTCCAGTTCTCAAGCAGTCGGACCGGCGTCAGTCAATGGCTTTC AGCATCCTTAACACACCCAAGAAGCTGGGAAATAGCCTTCTACGGCGAGGAACTTCAAAGAAGACCCCAG CAAAGGTTTCCCCCAATCCTCGCAGTGGAACCCGCCGCTCTCCACGCATCGCCACCACCACGGCTGGTGC TGCTACTGCTGCCACCACTCCTCGGGCCAAGGGCAAGGCAAAGCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217736 representing NM_001100691 Red=Cloning site Green=Tags(s) MTLHATRASTLLSWVNSLHVADPVETVLQLQDCSIFIKIINSIHDTKEGQQILQQPLPERLDFVCSFLQK NRKHPSSTQCLVSVQKVMEGSEMELAKMVMLFLYHSTMSSRNPRDWEQFEYGVQAELAVILKFMLDHEDS PNLTEDLENFLEKVPSTYPSTLSEELSPPSHQAKRKIRFLEIQRIASSSSENNFLSGSPSSPVGDILQTP QFQMRRLKKQLADERNNRDELELELSESLKLLTEKDAQIAMMQQRIDHLALLNEKQAASPQEPSELEELR GKNESLTVRLHETLKQCQNLKTEKNQMDRKISQLSEENGDLSFKVREFANHLQQLQGAFNDLIEEHSKAS QEWAEKQAHLESELSTALQDKKCLEEKSEILQEKISQLEDRAAQLQGSPAPEKGEVLGDALQLDTLKQEA AKLATDNTELQARVETLECERGKQEAQLLAERGHFEEEKRQLASLVADLQSSVSNLSQAKEELQQASQAQ GAQLTAQLASLTALNATLQQQDQELTSLKEQAKKEQAQMLQTLQEQEQAAQGLRQQVEQLSSSLKLKEQQ LEEAAKEQEAARQDHAQQLATIVEAREASVRERDAARQQLETLEKEKDAKLESLQQQLQASNEARDTAQT SVTQAQREKAELSQKIGELHACIEAAHQEQRQAQAHVTELEAQLKAEQQKATEREKVVQEKVQLQEQLQA LEETLKIVRGSLEEEKCRAADALKEQQRHATEMEAETRHLMEQREQEQKELEQEKAERKGLEARLQQLEE AHQAETEALRHELAGATAAQHGAESEREQLLREVESWQKRVEARQQEEARYGAMFQEQLMALKGEHGKIG QEEQKEAGEIHGEGQTGQQQSQLAQLHACLAKALQQVQEKEARAQKLLDDLSALREKMAATNKEVACLKA LVLKAGEQQAAASHELKEPPRAGNQESDWEEEQARPLGSTQAALKAVQREAEQMGGELERLRAALMQSQG QQQEVRGQQEREVARLTQERGQAQADLAQEKAAKAELEMRLQNTLNEQRVEFAALQEALTHAMTEKEGKD QELAKLREQEAAQISELKALQQTLEELKKKEKEHPTGGARGEDASGDGPGSQLHTPGKTEAPGPEVEALR AEISKLERQWQQQQQQVEGLTHSLESERACRAEQDKALETLQGQLEEKARELGHNQAASASAQRELQALR AKAQDHSKAEEEWKAQVARGQQEAERKSSLISSLEEEVSILNRQVLEKEGESKELKRLVVAESEKSQKLE ERLRLLQVETASSSARAAERSSALREEVQSLREEVEKQRVVSENLRQELASQAERAEESGQELKAWQEKF FQKEQALSALQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRFREEIEQSKQAAGGLQAELMRAQREL GELGSLRQKIVEQERAAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKY VQELAAVRTDAETHLAEMRQEAQSTSRELEVMTAKYESAKVKVLEERQRFQEERQKLTAQVEELNKKLTE SEQASRVQQQKLKAFQAQGGESQQEVQCLQTQLSELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQE LREQLQDLEQLQKDNKELRSETERLGRELQQAGLKTKEAEQACRHLTAQVRSLEAQVAHADQQLRDLGKF QVATDALKSREPQVKPQLDLSIDSLDLSLEEGTPCSVASKLPRTQPDGTSVPGEPASPISQRLPPKVESL ESLYFTPIPARGQAPLETSLDSLGDAFPDSGRKTRSARRRTTQIINITMTKKLELEEPDSANSSFYSTQS APASQANLRATSSTQSLARLGSPDDSNSALLSLPGYRPTTRSSARRSQTRTSSGAPHGRNSFYMGTCQDE PEQVDDWNRIAELQQRNRVCPPHLKTCYPLESRPALSLATITDEEMKTGDPRETLRRASMQPAQIAEGAG ITTRQQRKRVSSETHQGPGTPESKKATSCFPRPMTPRDRHEGRKQNSTADAQKKAAPVLKQSDRRQSMAF SILNTPKKLGNSLLRRGTSKKTPAKVSPNPRSGTRRSPRIATTTAGAATAATTPRAKGKAKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001100691
ORF Size	6276 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001100691.1, NP_001094161.1
RefSeq Size	7793 bp
RefSeq ORF	6279 bp
Locus ID	308870
MW	235 kDa

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