Med12 (NM_001193292) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217750 representing NM_001193292 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTCGGGATCTTGAGCTATGAACACCGACCCCTGAAGCGGCTGCGGCTGGGGCCTCCCGATG TGTACCCTCAAGATCCCAAACAGAAGGAGGATGAACTGACGGCTTTGAATGTAAAACAAGGTTTCAATAA CCAGCCTGCTGTCTCTGGGGATGAACATGGCAGTGCCAAGAACGTCAACTTCAATCCTGCCAAGATCAGT TCCAACTTCAGCAGCATCATCGCAGAGAAGTTAAGGTGTAATACTCTCTCTGACACTGGTCGAAGGAAGT CCCTAATGAACCAGAAGGACAACTTCTGGCTGGTGACTGCAAGATCTCAGAGTGCTATTAACACCTGGTT CACTGACCTAGCTGGCACCAAACCACTCACACACCTAGCCAAAAAGGTCCCCATTTTCAGTAAGAAGGAA GAAGTATTTGGGTACTTGGCCAAATACACAGTGCCTGTGATGCGGGCTGCTTGGCTCATTAAGATGACCT GCGCCTACTATGCAGCAATGTCTGAGACTAAGGTTAAGAAGAAAAACACCGCTGACCCCTTCACTGAATG GACTCAAATCATCACAAAGTACTTATGGGAGCAGCTACAGAAGATGGCTGAATACTATCGGCCAGGGCCT GCAGGAAGTGGAGTCTGTGGTTCTGCTATAGGACCTTTGCCCCATGATGTAGAGATGGCAATCAGGCAGT GGGACTACAATGAGAAGCTCGCCCTCTTCATGTTTCAGGATGGAATGCTGGACAGACATGAGTTCCTGAC TTGGGTGCTTGAGTGTTTTGAGAAAATACGCCCTGGAGAAGATGAATTGCTTAAATTGCTGCTTCCCCTA CTGCTTCGATACTCAGGGGAGTTCGTTCAGTCTGCCTATCTGTCCCGCCGCCTTGCCTACTTCTGTACCC GGAGATTGGCTCTACAGCTGGATGGTGTGAACAGCCACTCATCTCACGTTATATCGGCTCAGTCAACGAG TTCTCTGCCCACTACCCCAGCACCTCAACCCCCAACTAGCAATACACCCTCGACTCCCTTTAGTGACCTG CTTATGTGCCCTCAGCACCGTCCCTTGGTTTTTGGCCTCAGCTGTATCCTTCAGACCATCCTCCTGTGTT GCCCTAGTGCCCTAGTTTGGCACTACTCATTGACTGATAGCCGAATTAAGACTGGCTCACCACTTGACCA CCTGCCTATTGCTCCTTCCAACTTGCCTATGCCAGAGGGTAACAGTGCCTTTACTCAGCAGGTCCGTGCA AAATTACGAGAGATCGAACAACAGATCAAGGAGCGTGGACAAGCAGTTGAGGTTCGCTGGTCCTTTGATA AGTGCCAGGAAGCTACTGCAGGCTTCACCATTGGACGGGTGCTTCATACTTTAGAAGTGCTGGACAGCCA TAGTTTTGAGCGCTCTGACTTTAGTAACTCTCTTGACTCCCTTTGTAATCGAATCTTTGGACTGGGGCCT AGTAAGGATGGTCATGAGATCTCCTCTGATGATGATGCTGTGGTGTCGTTATTGTGTGAATGGGCTGTAA GCTGTAAGCGCTCCGGGCGGCACCGTGCTATGGTAGTAGCCAAACTCCTGGAGAAGAGACAAGCAGAAAT TGAGGCTGAGCGCTGTGGAGAATCTGAAGCAGCTGATGAGAAGGGTTCCGTCGCCTCTGGTTCCCTTTCT GCTCCTAGTGCACCCATTTTCCAGGATGTCCTCCTGCAGTTTCTGGATACACAGGCTCCCATGCTGACTG ATCCCCGAAGTGAGAGTGAGCGAGTGGAATTCTTTAACTTGGTACTGCTGTTCTGTGAACTGATCCGACA TGATGTCTTCTCCCATAATATGTACACCTGCACTCTCATCTCTCGGGGAGACCTTGCATTTGGAACACCT GGTCCTCGGCCCCCCTCTCCTTTTGATGATCCTGCAGATGATCCCGAGCGCAAGGAGGCTGAAGGCAGCA GCAGCAGCAAGCTAGAGGATCCAGGGCTCTCTGAATCTATGGACATCGACCCTAGTTCCAGTGTGCTTTT TGAGGACATGGAAAAGCCTGATTTCTCATTGTTCTCCCCTACTATGCCTTGTGAGGGGAAGGGAAGCCCA TCCCCTGAGAAGCCAGATGTCGAAAAGGATGTGAAACCCCCACCCAAAGAGAAGATTGAGGGGACACTTG GGATTCTGTATGACCAGCCACGACATGTGCAGTATGCCACACACTTTCCAATCCCACAGGAGGAGTCATG CAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTGGGGTGGGGAAGCAGCGAGATGATGCCCGCCAT GCCATCAAGAAGATTACCAAGGATATCCTGAAGGTTCTGAACCGCAAGGGGACAGCAGAAACTGACCAGC TTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACATTCTTAGGTGGGGAAGATGGGCAGAAGCGACG CCGCAACCGGCCTGAAGCCTTCCCCACTGCTGAAGATATTTTTGCTAAGTTCCAGCACCTTTCTCATTAT GACCAACATCAGGTCACGGCTCAGGTCTCCCGGAATGTTCTGGAGCAGATTACGAGTTTTGCTCTTGGCA TGTCATACCACTTGCCTCTGGTGCAGCATGTGCAGTTCGTCTTCGACCTCATGGAATATTCACTAAGCAT CAGTGGCCTCATCGACTTTGCCATTCAGCTGCTGAATGAGCTGAGTGTGGTTGAGGCAGAGCTCCTTCTC AAATCGTCTGATCTGGTGGGCAGCTATACTACCAGCCTGTGCTTATGCATCGTGGCTGTCCTTCGACACT ATCATGCCTGCCTCATCCTCAATCAAGACCAGATGGCGCAAGTGTTTGAGGGGCTCTGTGGCGTGGTGAA ACATGGAATGAACCGTTCAGATGGCTCCTCTGCAGAGCGCTGTATCCTTGCTTATCTCTATGATCTGTAT ACCTCCTGTAGCCATTTAAAGAGCAAATTTGGGGAGCTCTTCAGTGACTTTTGCTCAAAAGTGAAGAACA CCATCTACTGTAATGTGGAGCCATCAGAATCCAATATGCGCTGGGCACCGGAGTTCATGATTGACACTCT GGAGAACCCTGCTGCTCACACTTTCACCTACACGGGGCTAGGCAAGAGTCTTAGTGAGAACCCTGCCAAC CGCTACAGCTTTGTCTGCAATGCTCTTATGCACGTCTGTGTGGGCCACCATGATCCTGATAGGGTAAATG ACATCGCTATCCTTTGTGCAGAGCTCACCGGCTATTGCAAGTCACTGAGTGCAGAGTGGTTAGGAGTACT TAAGGCCTTGTGCTGCTCCTCTAACAATGGCACTTGTGGTTTCAACGATCTCCTGTGCAACGTAGATGTC AGTGACTTGTCTTTCCACGATTCCCTGGCTACTTTTGTTGCTATCCTCATCGCCCGTCAGTGTTTGCTCC TAGAAGACCTGATTCGCTGTGCAGCCATCCCTTCGCTCCTCAATGCTGCTTGTAGTGAGCAGGATTCTGA GCCAGGGGCCAGGCTTACTTGCCGAATTCTCCTCCACCTGTTCAAGACGCCACAACTCAATCCTTGCCAG TCCGATGGAAACAAACCTACCGTTGGAATCCGGTCCTCCTGTGACCGCCACCTGCTGGCTGCCTCCCAGA ACCGCATCGTGGATGGAGCTGTGTTTGCTGTTCTCAAGGCTGTGTTTGTACTCGGGGATGCGGAGCTTAA AGGTTCAGGCTTCACTGTGCCGGGAGGAACAGAAGAACTTCCAGAAGAGGAGGGAGGAGGTGGTAGTAGC GGTCGGAGGCAGGGTGGCCGCAACATCTCTGTGGAGACAGCAAGTCTGGATGTCTATGCCAAGTACGTGC TGCGAAGCATCTGCCAACAGGAATGGGTAGGAGAACGTTGCCTTAAGTCACTGTGTGAGGATAGCAATGA TCTACAAGACCCAGTGTTGAGTAGTGCCCAGGCCCAGCGCCTCATGCAGCTCATCTGCTACCCACATCGA CTGCTGGACAGCGAAGATGGAGAAAACCCCCAGCGGCAGCGCATTAAGCGGATTCTCAAGAACTTAGACC AATGGACCATGCGCCAGTCCTCTTTGGAGCTACAGCTGATGATCAAGCAGACCCCCAACACCGAGATGAA CTCTCTCTTGGAGAACATTGCCAAGGCCACAATTGAGGTTTTCCAACAGTCCGCAGAGACGGGATCATCC TCTGGCAGTACAGCAAGCAATATGCCCAGCAGCAGCAAGGCCAAACCTGTGCTCAGCTCTCTGGAGCGCT CTGGTGTATGGTTGGTGGCTCCTCTCATTGCCAAACTGCCCACTTCAGTTCAGGGCCATGTATTAAAAGC TGCTGGGGAGGAATTGGAAAAGGGTCAGCACCTGGGTTCTTCTTCCCGGAAAGAACGAGATCGACAAAAA CAGAAGAGTATGTCCCTGTTGAGCCAGCAGCCTTTCTTATCACTGGTGCTAACATGTCTGAAAGGACAGG ATGAACAACGTGAGGGACTCCTTGCCTCCCTCCACAGCCAGGTGCACCAGATTGTGATTAATTGGCGAGA AAACCAGTACTTAGATGATTGCAAACCAAAGCAGCTAATGCATGAGGCACTCAAACTGCGGCTCAACCTG GTCGGGGGCATGTTTGACACTGTGCAGCGTAGTACCCAACAGACTACAGAGTGGGCCCAGCTCCTCCTGG AGATTATCATCAGTGGCACTGTGGATATGCAGTCTAACAATGAACTCTTCACTACTGTGTTGGACATGCT GAGCGTGCTTATCAATGGAACGTTGGCTGCAGACATGTCCAGTATCTCACAAGGCAGCATGGAGGAAAAC AAACGTGCATATATGAACCTGGTGAAGAAGCTTCAGAAGGACTTGGGGGAGCGCCAATCAGACAGTCTGG AAAAGGTTCACCAACTGTTGCCACTACCCAAGCAGAACCGAGATGTCATAACCTGTGAGCCACAGGGCTC CCTTATTGACACCAAGGGCAACAAGATTGCTGGCTTCGATTCCATCTTCAAGAAGGAGGGTCTACAAGTT TCCACCAAACAAAAGATCTCTCCTTGGGAGCTTTTTGAGGGCCTGAAGCCATCAACAGCACCACTATCAT GGGCCTGGTTTGGCACAGTCCGAGTGGACCGCCGAGTGGCACGAGGGGAGGAGCAGCAACGACTGTTGCT CTACCATACCCACCTGAGGCCTCGACCCAGAGCCTATTACCTGGAGCCACTACCTCTGCCCCCAGAAGAT GAGGAGCCACCAGCCCCTGCCCTACTAGAGCCTGAGAAAAAGGCTCCTGAGCCCCCCAAGACTGACAAAC CAGGGACTGCTCCTCCCAGCACAGAGGAGCGCAAGAAGAAATCTACCAAGGGCAAGAAACGCAGTCAGCC AGCCACCAAGAGCGAGGACTATGGCATGGGGCCAGGTCGGAGTGGTCCCTATGGTGTGACAGTGCCTCCA GACCTTCTACACCATGCAAATCCAGGTTCTATATCCCACCTTAGCTACAGGCAGAGCTCCATGGGCCTGT ACACCCAAAACCAGCCACTGCCTGCTGGTGGCCCTCGTGTGGACCCATATCGCCCTGTGCGATTACCAAT GCAGAAGCTGCCAACTCGACCAACTTACCCCGGTGTGCTGCCTACAACTATGACTACTGTCATGGGCCTA GAACCCTCTTCTTATAAGACCTCTGTGTACCGGCAGCAGCAACCCACAGTGCCCCAGGGACAGCGCCTTC GCCAACAGCTCCAGCAGAGTCAGGGAATGTTGGGACAGTCATCTGTCCATCAGATGACTCCTAGTTCTTC CTATGGTTTGCAGAGTTCCCAGGGCTATACGTCCTATGTCTCTCATGTGGGATTGCAGCAACACACAGGC CCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCCTTATCAGAGCACCCACCCTTCTACCAATC CTACTCTTGTAGATCCTACCCGTCACCTGCAACAGCGGCCCAGTGGCTATGTGCATCAGCAGGCCCCAAC CTATGGGCATGGACTGACTTCCACTCAAAGGTTTTCACACCAGACACTGCAGCAGACACCAATGATGGGT ACCATGACTCCGTTGAGTGCCCAGGGTGTCCAGGCAGGCGTCCGTTCAACTTCCATCCTGCCTGAGCAGC AACAACAGCAACAACAGCAGCAGCAGCAACAGCAGCAGCAACAACAGCAACAGCAGCAACAGCAGCAACA GCAACAACAGCAACAACAGCAGTACCACATCCGACAGCAACAACAGCAACAGCAGATCCTAAGGCAACAG CAGCAACAGCAGCAGCAGCAGCAACAACAGCAGCAACAGCAGCAGCAACAACAGCAGCAACAACCACAAC AGCAGCAGCCACACCAGCAGCAGCAGCAGGCAGCACCTCCCCAACCTCAACCCCAGTCCCAGCCCCAGTT CCAGCGCCAGGGGCTTCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAACTTCAACAA CAGCTCTCTAATACCCAGCCACAGCCCAGTACCAACATATTTGGACGCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001193292
Insert Size	6564 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001193292.1, NP_001180221.1
RefSeq Size	6792 bp
RefSeq ORF	6564 bp
Locus ID	679693

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