Wnk1 (NM_001199095) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hsn2; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217783 representing NM_001199095 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGCACCGCAGAGAAGCAGAGCGGCACTCCTGGCTTCCTTTCGCCTCCGGCTCCAGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCCGCGGTCGCCGACTCCGGAATTGG CAGGACAGAGGAATACCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACT ACCCCCACGGAGCACCGATTTTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACGGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCTCAACCTAGTGTACCCGCAGTGGTGCCTCAGAGTGCTCCACCCGAGCC TCACAGAGAGGAAACTTTGACGGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCGCGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGTAGCAAAGAGGAGCCGCCGCCGTCCAGAAGTGGAAGTGGCAGCGGTGG TGCCAGTGCCAAAGAGCCGCAGGAGGAAAGGAACCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CGGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAAGCTGAAATGCTAAAGGGTCTTCAACATCCTAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAAACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGACTTCAATTTCTCCATACACGAACTCCCCCGATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACCGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAGTACGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACGTCTGAATAT CCATACTCAGAGTGCCAAAATGCTGCTCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTGAAGGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATCAAATTATGGCTACGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTCGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTATCATTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAACAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGTGTTTCCCAGGCAGGAATCCAGCCACT CTCTGTTGCTAGCACTGGCATACCCACTGCTCCCACCACATCCGCTTCAGTTTCTACTCAAGTAGAGCCT GAAGAACCCGAGGCAGATCAGCATCAACAGCTGCAGTATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACAGTTGACAGTGGTCAAGGATCATCTGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGCTCCCAACATGAGCAGGCACATTCTATTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCGTCGTGGCCGTAGCATGTCGGTTT GTGTTCCCCATCTTTCTGCTGTTCCCTCTCTATCCCGCATCTCTCCCAGTGCTCCTTCCACCCCACCACC AGTGCTGTCTGCACCTCTTTGTCCTTCCCTCCTTCGGAGTGCCCCTGAGGAAACTTTTGCGGAAAAACTC TCTAAAGCATTGGAGAGTGTCCTGCCTATGCACTCTGCCTCCCAGCGCAAGCACCGACGCTCCAGCCTGC CTTCCCTCTTTGTCACTACTCCTCAGTCCGTGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATC ACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCTACCTGTCCACCGAAA GTAGCCATTTCCCAGCGACGTAAGAGTACCTCCTTCCTGGAAGCTCAAACTCGCCACTTCCAACCCCTGC TGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTGGCAGCCCAACTAACTGGACACCAGAGGCCATAGT TATGTTGGGTACTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGATGCAGGTCCAACCTGTGTTTGAG ACAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAAGAAGCTCACTATTTTATTCCTC AGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCATGCGGCAGGACAGTATGAGGGTATTTCATA CAACTCACCAGTACTGTCAAGTCCTATGAAACAGATAACTGAACAGAAGCCAGTGCCTGGGTGCCCTGCC TCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTAGTAGGACACCTTCAGAATTTAAGATTAG ATTCTGGACCAAGTCCAGCATCACCCCTGTCTAGTATTTCTGCGCCTAACAGTACAGATGCTACACATTT GAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAACTCATAGCAATGAGAACAGAAGC AACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCCTCTTCAGGAGAAGGAGGTGGGATTTTACCTC AACGTGTTTACCGAAATCGACAGGTTGCAGTGGACTCAAGTCAGGAAGAACTGTCTCCTCAGTCAGTTGG ATTACACTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCATACCCCAGAATTGACCATTTCT GTGGTAGAACCTATGGGACAAAACTGGCCAGTAGGAAGCCCAGAATATTCCAGTGATTCCTCTCAAATTA CTTCTTCAGATATCAGTGATTTTCAATCACCTCCCCCTACAGGGGGAACAGCTGCACCTTTTGGCTCTGA CGTCTCATTGCCCTATATTCGCCTGCCTCAGACAGTGTTACAAGAATCCCCACTATTCTTCTGTTTCCCC CAAGGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGAGGATCTGCACTCCATCCACAGG CACAGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTAGCAGGGGTTCTATCTTCACAACCCGTCCAACA TCCTCAGCAGCAGGGAATACAGCCGACTGTACCTCCTCAACAGGCAGTACAGTATTCACTTCCACAGGCA GCGTCTTCCAGTGAAGGCACTGTCCAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTAGTA CACAGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAATAACACAGTCACAACCTACTCAACCTGT TCCATTGGTTTCCTCTGTGGACAGTGCCCATTCAGATGTTGCTTCTGGTATGAGTGATGGCAATGAGAAT GCTCCTTCATCCAGCGGGAGGCATGAAGGGAGGACGACAAAACGTCATTATCGAAAATCTGTAAGAAGTC GTTCTCGTCATGAAAAGACTTCTCGCCCGAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGT AGTAGAATGTCAATTGGAGACTCATAATCGGAAAATGGTTACATTTAAATTTGACCTAGATGGTGACAAC CCTGAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCCATAGAGAGAGAGTCTTTTGTGG CTCAAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAGCGAGGATGTCAGTGTGGAGCCTGAGGG TGACCAGGGATTGGAGAGTCTACAAGGAAAGGATGACTATGGCTTTCCAGGTTCTCAGAAATTAGAAGGA GAGTTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACAC AAGTTGTTCATTCTGCTGGAAGACGATTTATAGTGAGTCCAGTTCCAGAAAGTCGATTACGAGAATCAAA AATTTTCACCAGTGAAATACCAGATCCAGTTGCGGCCTCTACATCTCAGGGCCCTGGAATGAACTTATCT CACTCCGCTTCATCCCTTAGTCTCCAGCAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGAC CCAATACAGCACCTCCAAATTTTAATCACCCAGGACCAACATTTTCTCCCTTTTTGACTAGCATTGCTGG TGTCCAAACTGTAGCAGCATCCACACCATCAGTATCAGTCCCTATAACAAGCAGCCCTCTTAATGACATT TCCACATCAGTGATGCAGTCAGAGGGTGCACTGCCCACTGACAAAGGCATTGGGGGAGTTACCACCAGCA CAGGTGTGGTAGCTTCAGGTGGTCTCACCACATTGTCTGTGTCTGAGACACCAACACTTTCCAGTGCAGT TTCAAGTTCTACAGCGCCTGCAGTTGTCACAGTGTCTACAACATCCCAACCAGTTCAGGCTTCCACATCT GGGAGCATTGCTTCTAGTACAGGCAGTTTTCCTTCTGGAACATTTTCCACAACTACAGGTACCACAGTGA GTAGTGTAGCTGTCCCAAATGCTAAGCCTCCAACTGTATTACTGCAACAGGTAGCAGGTAATACTGCTGG GGTAGCCATAGTGACATCAGTTTCTACTACCACTCCATTCCCAGCCATGGCTTCACAGCCATCCCTTCCA CTTGGTAGCAGCACATCTGCTCCTACACTAGCTGAAACAGTGGTGGTTAGTGCACACTCACTAGACAAGG CATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGCGCACCGTCCTCTTCTTCCTCCTCTGGAAC AGCAGTGTCTAGTTCTGTTTCTCAGCCTGGTATTGTGCATCCTTTGGTCATCTCATCAGCTATAGCTTCT ACACCTGTCCTCCCACAACCAGCAGTACCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCCCAC CCTTGGTACAACCTGTTGCTAATGTGCCTGCTGTACAGCAAACACTCATTCATAGTCAGCCTCAACCAGC TCTACTTCCCAACCAGCCTCACACACACTGTCCCGAAATGGATGCTGATACCCAGTCCAAAGCTCCTGGG ATCGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCGCTCTTTAGTGAGCATAGCTCATCTGGAACTC AGCATGCTTCTGTCTCCCTGGAGACACCCTTAGTAGTAGAGACTGTCACACCGGGCATCCCTACCACTGC TGTAGCACCAAGTAAACTCATGACTTCCACTACAAGCACTTGCTTGCCACCAACCAATTTACCCTTAGGA ACAGCTGGCATGCCAGTTATGCCAGTGGGCACACCTGGTCAAGTTTCTACCCCAGGCACACATGCCTCAG CCCCAGCCAGCACCGCAACAGGAGCAAAACCTGGAACAACTCCCCCAAAGCCATCTTTAACTAAGACTGT GGTGCCGCCAGTGGGTACTGAGCTTTCGGCTGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCCGGA CCGTCTCTAATTCAGACCCAGCAACCCCTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAG AGACTATTCCAGTGACACCCGCAGTTGGTCCTTTGTCTACGATGTCTTCAACGGCAGTTACAGAAGCAGG AAGTCAACCTCAGAAGGATGGCACAGAAGTTCATGTCACAGCAAGTAGCTCAGGAGCTGGTGTTGTTAAG ATGGGGAGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGAAAGAGCGTAAAAATAGGTCAGAAGATA CAAAGTCGGTTCATTTTGAATCTAGTACATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTAC CCTGGTAAAGCCAGAGCCTAATGGAATTACTGTTTCTGGCATCTCCTTAGATGTGCCAGACAGTACCCAT AGAACACCGACCCCAGAAGCAAAGTCAGAAACAGGACAGCCTACCAAGGTTGGACGTTTTCAGGTGACAA CTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGA GGGACCAGTGACCTCTCCTTTCAGAGATTCAGAACAAACTGTTATTCCTGCTGCGATACCAAAGAAGGAG AAGCCTGAGCTGGCAGAACCTTCCCATCTGAATGGGCCATCTTCAGACTTGGAGGCTGCCTTTCTAAGTA GGGGTGGAGAGGATGGATCAGGTAGTCCTCACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCATCCA GACTCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCTTACATGAGTAGTGACAATGAGTCCGACATT GAAGATGAAGACTTAAGGTTAGAACTGCGACGACTACGGGAAAAACATCTTAAAGAGATTCAGGACCTGC AGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACCAAACTGGGCAAGGTTCCCCCTGCTGTCATTAT TCCCCCAGCTGCTCCTCTGTCAGGGAGAAGAAGGAGGCCTACTAAAAGCAAAGGCAGCAAGTCCAGTCGC AGCAGCTCATTGGGCAACAAAAGCCCTCAGCTTTCAGGTAACCTGTCTGGTCAGAGTGGAACTTCAGTCT TAAACCCCCAACAGACCCTCCATCCCCCAGGCAATACCCCAGAGACTGGACACAACCAGCTGTTACAGCC TCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTACCA AGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAACACCGTTGGAGGAACAGTGAGTAGCCAGGCTG CCCAGGCTCAGCCTCCTGCCATGACTTCCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTTGGT AGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTATGAG GGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGTGCATTTCCATGACCTCAAACATGGGTG GCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCACTTCACCAAGTCCATGTGCCCCCCACA GCAGTATGGTTTTCCAGCTGCCCCATTCGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCACAGCCA CTTGGTCAGTTCCAACCTGTAGGAACCACATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGTCCA TCAGCAACCCCCCAGGTTCCAACCTACGGACCACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199095
Insert Size	7878 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199095.1, NP_001186024.1
RefSeq Size	10470 bp
RefSeq ORF	7878 bp
Locus ID	116477
UniProt ID	Q9JIH7
Gene Summary	catalyzes the phosphorylation of myelin basic protein in vitro; has autophosphorylation activity; may regulate the thiazide-sensitive Na-Cl cotransporter NCC [RGD, Feb 2006] Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame compared to variant 1. This variant represents the exon combination of the brain and spinal cord variant inferred from the mouse sequence described in Figure 2F of PubMed ID 18521183. This variant encodes isoform 2, which is shorter than isoform 1 but has the same N- and C-termini. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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