Vwf (NM_053889) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217794 representing NM_053889 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTTTCGGATATGAGATCTGCCTGTTAGTTCTGGCCCTCACCTGGCCAGGGACCCTCTGCACAG AAGATCCTCGTGACAGGCCGTCGACAGCCCGATGCAGCCTCTTTGGGGATGGCTTCATCAACACTTTCGA TGAAGCCATGTACAGTTTTGCAGGAGGCTGCAGTTATCTTCTGGCTGGGGACTGCCAGAAACGTTCCTTC TCAATTCTCGGGAACTTCCAAGATGGCAAGAGAGTGGGCCTGTCTGTGTATCTTGGGGAGTTTTTTGACA TCCATTTGTTTGCCAACGGCACCGTGATGCAGGGGGACCAAAGCATCTCCATGCCCTATGCCTCCAAGGG ACTCTATGTAGAGCATGAGGCTGGGTACTACAAAATTTCCAGTGAGGCCTTCGGCTTTGCGGCCAGAATT GACGGCGACGGCAACTTCCAAGTCCTGATGTCAGACAGACACTTCAACAAGACCTGTGGGCTGTGTGGTG ACTTCAACATCTTCGCTGAAGATGACTTTAGGACGCAGGAGGGGACCTTGACCTCAGACCCCTACGACTT TGCCAACTCCTGGGCCCTGAGTAGTGAGAACCAGCGGTGTAAACGGACATCTCCTCCCAGCACTAACTGC ACCAGCTCCTCTGGGGACATGCAGCAGGCCATGTGGGAGCAGTGCCAGTTACTGAAGACGGCGTCGGTGT TTGCCCGCTGCCACCCTCTGGTGGATCCCGAGCCCTTCGTGGCTCTCTGTGAGAAGACTTTGTGTACATG CGTCACGGGGCCAGAGTGCGCGTGTCCTGCTCTCCTTGAGTATGCCCGAACCTGTGCCCAGGAAGGGATG GTGCTGTATGGCTGGGCTGACCACAGTGCCTGTCGCCCAGCATGCCCGGCTGGCATGGAGTATAAGGAGT GTGTGTCTCCTTGCACCAGAACCTGCCAGAGCCTGCCTATCAATGAAGTGTGTCAGCAGCAATGTGTAGA TGGCTGTGGCTGCCCTGAGGGAGAGCTCCTGGACGAAGACCGCTGTGTGCAGAGCTCTGACTGTTCTTGT GTACACGCCGGAAAGCGGTACCCACCTGGCACCTCCCTCCCTCAAGACTGCAACACTTGCATCTGCAGAC ACAGTCTCTGGATCTGCAGCAATGAGGAATGCCCAGGGGAGTGTCTTGTCACAGGCCAATCCCACTTCAA GAGCTTCGACAACAGACACTTCACCTTCAGTGGCATCTGCCAGTACCTGCTGGCCCGGGACTGCCAAGAC CACTCTTTCTCCATTGTCATAGAGACCATGCAGTGCGCTGATGACCCTGATGCTGTCTGCACCCGCTCAG TCAGTGTGCGGCTGTCTGCCCTGCACAACAGCCTCATGAAGCTGAAGCACGGGGGAGGGGTGGCCATCGA TGGTCAGGATGTCCAGATTCCCTTCCTGCAAGGTGACCTCCGTATCCAGCACACGGTGATGGCTTCTGTA CGCCTCAGCTATGGGGAGGACCTGCAGATGGAATGGGATGGCCGCGGGCGGCTACTGGTTAAGCTGTCCC CAATCTATTCTGGGAAGACCTGTGGTTTGTGTGGGAATTACAATGGCAACAAGGGAGACGACTTCCTCAC GCCGGCCGGCCTGGTGGAGCCTCTGGTGGTAGACTTTGGAAACGCCTGGAAGCTTCAAGGGGATTGTCCG GACCTGCACAGACAACACAGCGACCCCTGCAGCCTGAACCCACGCTTGACCAGGTTTGCGGAGGAGGCTT GTGCGCTCCTGACGTCCTCCAAGTTCGAGACCTGCCACCATGCGGTCAGCCCTCTGCCCTACCTGCAGAA CTGTCGTTATGATGTTTGCTCCTGCGCTGACAGCCAGGATTGCCTGTGTAGCGCAGTAGCTAACTACGCT GCTGCGTGTGCCCGAAAAGGCGTGCACATCGGGTGGCGGGAGCCAGACTTCTGTGCTCTGAGCTGCCCAC AGGGCCAGGTGTACCTGCAGTGTGGCAATTCCTGCAACATGACCTGCCGCTCCCTCTCCCTTCCGGATGA AGAATGCAGTGAAGTCTGTCTTGAAGGCTGCTTCTGCCCCCCAGGGCTCTACCAGGATGAAAGAGGGGAC TGTGTGCCCAAGGCCCAGTGTCCCTGTTACCACGACGGTGAGCTCTTCCAGCCTGCAGACATCTTCTCAG ACCACCACACCATGTGTTACTGTGAAGATGGCTTCATGCACTGTACCACGAGTGGTGCCTCGGGGAGCCT GTTGCCTAACATTGTCCTCAGCAGCCCCTTGTCTCACCGCAGCAAAAGGAGCCTCTCCTGTCGGCCACCC ATGGTCAAGTTGGTATGTCCTGCTGACAACCCACGGGCTCAAGGGCTGGAGTGTGCTAAGACGTGCCAGA ACTATGACCTGGAATGCATGAGTCTGGGCTGTGTGTCAGGCTGCCTCTGTCCCCCAGGCATGGTCCGGCA CGAAAACAGGTGTGTGGCCTTGGAGCGCTGTCCCTGCTTCCACCAGGGTGAGGCGTACTCCCCAGGAGAT ACAGTGAAGATCGGCTGCAACACCTGTGTCTGCCGGGACCGGAGGTGGAACTGTACAACCCACGTGTGTG ACGCCACTTGCTCTGCCCTTGGTATGGCTCACTACCTCACCTTCGATGGTCTCAAGTACCTGTTCCCTGG GGAGTGTCAGTACGTTCTGGTGCAGGATTACTGTGGCGGTAGCTCTGGGACCTTTCGGATCCTGGTGGGG AATGAGGGTTGCACTTATCCCTCGGTGAAATGCAAGAAACGGGTGACCATCCTGGTGGATGGAGGGGAGA TTGAACTGTTCAATGGAGAGGTGAATGTTAAGAGACCTCTGAAAGATGAGTCTCACTTTGAGATGGTGGA GTCGGGCCAGTACGTCATCCTGCTGCTGGGTCAGGGCCTTTCTGTGGTCTGGGACCACCGCCTCAGCATC TCCGTGGTCCTGAAGTACACATACCAGGAACACGTGTGTGGCCTGTGTGGGAACTTCGATGGTATCCAGA ACAATGACTTCACCAGCAGCAGCCTTCAGGTGGAGGAAGACCCCATTGACTTTGGGAACTCCTGGAAAGT GAGCCCACAGTGTGCCGACACAAGAAAGCTGTCACTAGACGTTTCCCTTGCCGCCTGCCACAACAATATC ATGAAACAGACAATGGTGGACTCATCTTGCAGAATCCTTACCAGTGACATCTTCCAGGGCTGCAACAGGC TGGTGGACCCCGAGCCATACCTGGACATCTGCATTTATGACACTTGCTCCTGTGAGTCCATCGGGGACTG CTCCTGTTTCTGTGACACCATCGCTGCCTATGCCCACGTGTGCGCCCAGCATGGCCAGGTGGTAGCCTGG AGGAAACCCACACTGTGCCCCCAGAGCTGTGAAGAAAGGAACGTTCGGGAAAACAGCTATGAGTGTGAGT GGCGCTATAACAGCTGTGCACCCGCCTGCCCGGTCACATGCCAGCACCCTGAGCCTCTGGCTTGTCCTGT ACAGTGTGTGGAGGGCTGCCATGCACACTGCCCTCCAGGGAAAATCCTGGATGAACTTCTGCAGACCTGT GTGGATGCCCAAGACTGCCCTGTGTGTGAGGTGGCTGGTCGGCGCTTGGCTTCCGGGAAGAAAATCACCC TGAGTCCCAATGACCCTCAGCACTGTCAGAATTGTCACTGTGATGGTGTGAACCTTACCTGTGAAGCCTG CCAAGAGCCCGGAGGCCTGGTGGTACCCCCAACAGATGCCCCAGTCAGCTCCACCACCCCATATGTTGAG GACACCCCCGAACCCCCCCTGCACAACTTCTACTGCAGCAAGCTGCTGGATCTCGTCTTCCTCCTGGATG GCTCCTACAGGCTGTCTGAGGCTGAGTTTGAAGTGCTCAAAGCTTTTGTGGTGGGTACGATGGAGAGGCT ACACATCTCTCAGAAGCGCATCCGCGTGGCAGTGGTAGAGTACCATGATGGCTCCCATGCCTACCTTGAG CTCAGGGCCCGGAAGCGACCCTCAGAGCTTCGGCGCATCGCCAGCCAGATTAAGTATGTGGGCAGCCAGT TGGCCTCTACCAGTGAGGTTTTGAAGTACACACTGTTCCAGATCTTTGGCAAAATTGACCGTCCTGAAGC CTCCCGTGTCATTCTGCTCCTGACCGCTAGCCAGGAGCCCCAACGGATGGCTAGGTATTTCACCCGCTAT CTCCAAGGTTTCAAGAAGAAGAAGGTCATTCTGATCCCTGTGGGCATTGGGCCGCACGCCAACCTCAAAC AGATCCGCCTCATTGAGAAGCAGGCCCCTGAGAACAAGGCCTTTCTGCTTAGTGGGGTGGATGAGCTGGA GCAGAGAAGAGATGAGATAATTAACTACCTCTGTGACCTTGCCCCCGAGGCCCCAGCCCCTACTAAGCCT CCACAGGTAGCACACATCACTGTGAGCCCTGGTATCTCTGGGGTTTCATCACCAGGACCCAAAAGGAAGT CTCTGGTTCTGGATGTGGTGTTTGTCCTGGAGGCGTCAGACGAAGTTGGTGAAGCCAACTTCAATAAGAG CAAGGAGTTCCTGGAGGAGGTTATCCAGCGCATGGATGTGAGCCCGGCTGGGACGCACATCGCAGTTCTG CAGTACTCATACACAGTAAACGTGGAGTACACCTTCAAGGAGGCCCAGTCCAAGGAGGATGTGCTGCGAC ATGTGCGAGAGATCCGCTACCAGGGGGGCAACAGGACAAACACTGGGCAGGCCCTGCAGTACCTTTCTGA GCACAGCTTCTCTCCCAGCCAGGGGGACCGGGAGCAGGCACCTAACCTGGTCTACATGGTCACGGGGAAC CCTGCCTCTGATGAGATCAGGAGGTTGCCTGGAGACATCCAGGTGGTACCCATTGGGGTGGGCTCCAGGG CCAACCTGCAGGAACTGGAGAGGATCAGCAGACCCATCGCTCCCATCTTCATCCAAGACTTTGAGACACT TCCCCGAGAGGCTCCCGACCTGGTCCTGAGGACGTGCTGCTCCAAGGAGGGGCTGCAGCTGCCCACCCTC CCCCCTCTCCCTGACTGCAGCCAACCCCTGGATGTGGTCCTGCTCCTGGATGGCTCCTCTAGCTTGCCTG CATCTTCCTTTGATGAGATGAAGAGTTTTGCCAAAGCTTTCATCTCAAAGGCCAACATTGGGCCCCATCT CACTCAGGTGTCCGTGATACAATACGGAAGCATCAATACCATCGATGTACCGTGGAATGTGGCCCAGGAG AAAGCCTATCTACAGAGTTTGGTGGACCTCATGCAGCAGGAGGGCGGCCCCAGCCAGATTGGGAATGCTC TGGCCTTTGCCGTGCGCTATGTAACTTCACAGATCCATGGAGCCAGGCCTGGAGCCTCCAAAGCAGTGGT CATGATCATCATGGATACCTCCTTGGATTCGGTGGACACAGCAGTGGATGCTGCCAGATCCAACCGGGTG GCAGTGTTCCCCATTGGGGTTGGGGATCGATATGATGAAGCCCAGCTGAGGATCTTGGCAGGCCCTGGGG CCAGCTCCAATGTGGTAAAGCTCCAGCAAGTTGAAGACCTCCTCACCATGGTCACCCCGGGAAACTCCTT CTTCCACAGACTGTGTTCTGGGTTTTCTGGAGTTTGTGTGGATGAGGATGGGAACGAGAAGAGGCCTGGA GACGTCTGGACCTTGCCAGATCAGTGCCACACAGTGACTTGCTTGGCAAATGGTCAGACCTTGTTGCAGA GTCATCGGGTCAATTGTGACCATGGACCCCGGCCTTCATGTTCCAACAGCCAGTCTCCCGTTCGGGTGGA GGAGACCTGTGGCTGCCGCTGGACCTGCCCTTGTGTGTGCACGGGCAGTTCCACTCGGCACATCGTCACC TTCGATGGACAGAATTTCAAGCTTACCGGCAACTGCTCCTACGTCATCTTTCAAAACAAGGAGCAGGACC TGGAAGTGGTCCTCCATAATGGGGCCTGCAGCCCTGGGGCCGTGCAAACCTGCATGAAGACCATCGAGGT TAAGCATGCTGGCCTCTCTGTTGAGCTGCGCAGTGACATGGAGATGGCAGTGAATGGGAGACCCGTCCTT GCCCCGTACGTGGGTGGAAATATGCAAGTCAGCATCTATGGTGCTATCATGTATGAAGTCAGGTTCACCC ATCTTGGTCACACTCTCACATTTACTCCACAAAACAACGAGTTCCAACTGCAGCTTAGCCCCAAGACCTT TGCTTCGAAGATGTATGGTCTCTGTGGAATCTGTGACGAAAATGGGGCCAATGACTTCACATTGCGAGAT GGCACGGTCACCACAGACTGGAAGAGGCTTATCCAGGAATGGACCGTGCAGCAGCCAGGGAGCACATGCC AGTCTGTTCCTGAAGAGCAGTGTCCCATCTCTGACAGCTCCCACTGCCAGGTCCTCCTCTCAGCATCGTT TGCTGAATGCCACAAGGTCATCGCTCCAGCCACATTCCATACAATCTGCCAGCAAGACAGTTGCCACCAG GAGCGAGTGTGTGAGGTGATTGCTTCTTACGCCCATCTCTGTCGGACTAATGGAGTCTGTGTTGATTGGA GGACAACTGACTTCTGTGCTATGTCATGCCCACCATCCCTGATATATAACCACTGTGAGCGTGGCTGCCC TCGGTACTGTGATGGAAACACTAGCTTCTGTGGCGACCACCCCTCAGAAGGCTGCTTCTGCCCCCAACAC CAAGTCTTGCTGGAAGGCAGCTGTGTCCCCGAAGAGGCCTGCACTCAGTGTGTTGGGGACGATGGAGTCC GACATCAGTTCCTAGAGACCTGGGTCCCAGACCATCAGCCCTGTCAGATCTGTATGTGCCTCAGTGGGAG GAAGATTAACTGCACTGCACAGCCATGTCCCACAGCCCGGGCTCCCACCTGTGGCCCTTGTGAAGTGGCT CGTCTCAAGCAGAGTGCAGACCTGTGCTGCCCAGAGTATGAGTGTGTGTGTGACCTGGTCAACTGCAACT TGCCACCAGTGCCTCCGTGTGAAGGAGGACTCCAGCCAACCCTGACCAACCCTGGAGAATGCAGACCCAC CTTTACCTGTGCCTGCAGGAAAGAAGAGTGCAAAAGAGTGTCCCCACCCTCCTGCCCCCCTCACCGGACA CCCACCCTTCGGAAGACCCAGTGCTGTGATGAGTATGAATGCACTTGCAGCTGTGTCAACTCCACGCTGA GCTGCCCACTTGGCTACCTGGCCTCGGCCACCACCAATGACTGTGGCTGCACCACAACCACCTGTCTCCC TGACAAGGTTTGTGTCCACCGAGGCACCGTCTACCCTGTGGGCCAGTTCTGGGAGGAGGGCTGTGACACA TGCACCTGCACCGACATGGAGGATACTGTTGTGGGCCTGCGTGTGGCTCAATGCTCTCAGAAGCCCTGTG AAGACAGCTGTCAGCCAGGTTTTTCTTATGTTCTCCACGAAGGGGAGTGCTGTGGAAAGTGCCTGCCGTC TGCTTGCAAGGTGGCTGGCTCACCGAGGGGTGATTCCCTCTCTTCCTGGAAGAGCGTTGGGTCTCAGTGG GCTGTTCCTGAGAACCCCTGCCTCATCAATGAGTGTGTCCGAGTGGAGGACGCAGTGTTTGTGCAGCAGA GGAACATCTCCTGCCCACAGCTGGCTGTCCCTACCTGTCCCACGGGCTTCCAGCTGAACTGTGAGACCTC AGCATGCTGTCCCAGCTGTCACTGTGAGCCTGTGGAGGCCTGCCTGCTCAATGGCACCATCATTGGGCCC GGGAAGAGTGTGATGGTTGACTTATGCACGACCTGCCGTTGCATTGTTCAGAGAGGAGCCATCTTCAGAT TCAAGCTGGAGTGCAGAAAGACCACCTGTGAGGCCTGCCCTGTGGGCTATCGGGAGGAGAAGAGTCAGAG CGAATGCTGTGGCCGGTGCCTGCCTACGGCTTGCACTATTCAGCTGAGAGGAGGACGGGTCATGACCCTG AAGCAAGATGAGACATTCCAGGATGGCTGTGACAGTCATCTGTGCAGGGTCAACGAGAGAGGGGAGTACA TTTGGGAGAAGAGGGTCACGGGCTGCCCCCCATTCGATGAACGCAAGTGTCTGGCTGAAGGAGGCAAAAT CGTGAAAATTCCAGGCACCTGCTGTGACACGTGTGAGGAGCCCGATTGCAAAGACATCACAGCCAAGGTG CAGTATATCAAAGTGGGAGATTGTAAGTCGGAAGAGGAAGTGGACATTCACTATTGCCAGGGAAAGTGCG CCAGCAAAGCTGTGTACTCCATTGACATCGAGGATCTTCAGGAACAGTGCTCCTGCTGCTGGCCCTCAAG TACAGAGCGCATGCGTGTGCCCTTGCTCTGCACCAATGGCTCGGTTGTGCACCACGAGGTCATCAATGCC ATGCAATGCAGATGTTCTCCCCGTAATTGTGGCAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053889
Insert Size	8439 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053889.1, NP_446341.1
RefSeq Size	8803 bp
RefSeq ORF	8439 bp
Locus ID	116669
Gene Summary	human homolog is a plasma glycoprotein that mediates platelet adhesion to damaged blood vessels and stabilizes blood coagulation factor VIII [RGD, Feb 2006]

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