Ank3 (NM_031805) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ANK-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200065 representing NM_031805 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGAAGAGGCAAAGGAGAAAACCGCCAAGCCTGCTCATAGGAAGAGGAAAGGGAAAAAGTCGGATG CCAATGCAAGTTACTTGAGAGCAGCTCGGGCGGGGCACCTGGAAAAGGCCCTTGACTACATCAAAAATGG AGTGGACGTCAACATCTGTAACCAGAATGGGTTGAATGCGCTCCATCTTGCTTCCAAAGAAGGCCATGTG GAAGTGGTCTCCGAGCTGCTGCAGAGAGAAGCCAACGTCGATGCAGCCACAAAGAAAGGAAACACAGCCT TACACATCGCATCCTTGGCTGGACAAGCGGAAGTGGTCAAGGTCTTGGTTACGAACGGAGCGAACGTCAA CGCACAGTCTCAGAATGGCTTCACGCCGCTGTATATGGCGGCCCAGGAGAACCACCTGGAAGTCGTCAGG TTTCTTCTGGACAATGGTGCCAGCCAAAGCCTGGCTACAGAGGATGGCTTCACGCCATTGGCCGTGGCTC TTCAACAAGGTCACGACCAAGTCGTATCCCTCCTGCTCGAGAACGACACGAAGGGAAAAGTGCGCCTCCC AGCCCTCCACATCGCGGCCCGGAAAGATGACACCAAAGCGGCAGCTCTGCTCCTACAGAACGACACCAAT GCAGACATAGAGTCAAAGAGTGGCTTCACCCCACTCCACATAGCTGCCCACTACGGGAATATCAATGTGG CCACGTTGCTGTTAAACCGAGCGGCTGCCGTGGACTTCACCGCACGGAATGACATCACTCCTTTGCATGT TGCCTCGAAACGGGGAAACGCAAATATGGTAAAGCTGTTGCTCGACCGGGGTGCGAAGATCGACGCCAAG ACCAGGGACGGTCTGACCCCGCTGCACTGCGGGGCGAGAAGTGGCCACGAGCAGGTGGTAGAGATGTTGC TGGACAGAGCCGCCCCCATCCTTTCGAAAACCAAGAATGGACTGTCCCCACTGCACATGGCCACACAAGG AGACCATTTAAACTGCGTCCAACTCCTCCTCCAGCACAACGTGCCCGTGGATGACGTCACCAACGACTAC CTGACCGCCCTCCACGTGGCGGCCCACTGCGGCCATTACAAAGTTGCCAAGGTTCTTTTGGATAAGAAAG CTAACCCCAATGCCAAAGCTCTGAATGGCTTCACCCCTCTCCATATCGCCTGCAAAAAGAACCGAATCCG AGTAATGGAACTCCTTTTGAAGCACGGTGCATCTATTCAAGCCGTAACCGAGTCGGGCCTTACCCCAATC CATGTCGCTGCCTTCATGGGACATGTAAATATCGTGTCACAGCTAATGCATCATGGAGCCTCCCCAAACA CCACCAACGTGAGAGGAGAAACAGCATTGCATATGGCGGCTCGGTCGGGGCAAGCAGAAGTGGTGCGGTA TTTGGTACAAGATGGAGCTCAGGTAGAAGCAAAAGCTAAGGATGACCAGACCCCACTCCACATTTCAGCC CGACTCGGGAAGGCTGACATAGTGCAACAACTGTTACAGCAAGGAGCGTCCCCGAATGCAGCAACAACTT CCGGGTACACCCCCCTTCACCTTTCGGCCAGAGAGGGGCATGAGGATGTAGCTGCCTTCCTTCTGGACCA TGGAGCATCTTTATCCATAACAACAAAGAAGGGATTCACGCCTCTGCACGTGGCTGCAAAATATGGGAAG CTTGAAGTGGCAAGTCTCCTGCTGCAGAAGAGTGCGTCTCCTGATGCCGCTGGGAAGAGCGGGCTAACTC CACTGCATGTAGCAGCACATTACGATAATCAGAAAGTGGCCCTTCTGCTTTTGGACCAGGGAGCCTCACC CCACGCAGCTGCAAAGAACGGTTATACGCCACTGCACATCGCCGCCAAGAAGAACCAGATGGACATAGCC ACGTCCCTGCTGGAGTACGGAGCTGATGCAAACGCCGTTACCCGGCAAGGGATTGCGTCCGTCCACCTTG CAGCGCAGGAAGGGCACGTGGACATGGTGTCGCTGCTCCTCAGTAGAAATGCGAACGTCAACTTGAGCAA TAAGAGCGGCCTCACCCCACTCCACCTGGCGGCTCAAGAAGACCGAGTGAACGTGGCTGAGGTCCTTGTC AACCAAGGGGCCCACGTGGATGCACAGACAAAGATGGGCTACACGCCGCTCCATGTGGGCTGTCACTATG GAAACATCAAAATAGTCAATTTTCTGCTGCAGCATTCTGCGAAAGTTAATGCCAAGACAAAGAATGGATA CACGCCACTGCACCAGGCAGCCCAGCAGGGCCACACACACATCATCAACGTCCTGCTCCAGAACAACGCT TCCCCCAATGAACTCACTGTGAATGGGAACACAGCCCTGGCCATCGCCCGACGCCTTGGCTACATCTCGG TGGTTGACACCCTAAAGGTCGTGACAGAGGAAATTATGACCACCACTACCATCACGGAGAAGCACAAAAT GAATGTCCCAGAAACGATGAATGAAGTCCTCGACATGTCAGACGATGAAGGTGAAGATGCCATCACAGGG GACACTGACAAGTATCTCGGGCCACAGGACCTCAAGGAGCTGGGCGATGACTCCCTGCCTGCCGAGGGCT ATGTGGGCTTCAGTCTCGGAGCCCGTTCTGCCAGTCCCAAGATAAGTTCGGATAGGTCCTACACCCTGAA CAGAAGCTCCTATGCGCGGGACAGCATGATGATCGAGGAGCTTCTGGTGCCATCCAAAGAGCAGCACCTT CCATTCACGAGGGAGTTTGACTCCGACTCCCTCAGACACTACAGTTGGGCAGCAGACACACTGGACAATG TGAACCTGGTCTCCAGCCCTGTGCATTCTGGGTTTCTGGTTAGCTTTATGGTGGACGCGAGAGGGGGCTC CATGCGAGGAAGCCGCCACCACGGGATGCGGATCATCATCCCTCCGCGCAAGTGTACGGCCCCCACCCGC ATCACTTGCCGCCTGGTAAAGAGACATAAACTGGCCAACCCACCCCCCATGGTGGAAGGAGAGGGATTAG CCAGTAGGCTGGTAGAAATGGGTCCTGCGGGGGCACAATTTTTAGGCCCGGTCATAGTGGAAATCCCTCA TTTTGGGTCCATGAGAGGAAAGGAGAGGGAACTCATTGTCCTCCGGAGCGAGAACGGAGAGACCTGGAAG GAACACCAGTTTGACAGCAAAAACGAAGACCTCTCGGAGCTCCTCAATGGCATGGACGAAGAACTTGACA GCCCAGAAGAGTTGGGCACAAAGCGCATCTGCAGAATCATCACGAAGGATTTCCCCCAGTATTTCGCTGT GGTTTCCCGGATTAAGCAGGAAAGCAACCAGATTGGTCCCGAAGGTGGGATTCTGAGCAGCACCACGGTC CCCCTCGTCCAGGCGTCTTTCCCAGAGGGTGCCTTAACCAAAAGGATCCGTGTGGGTCTGCAGGCTCAGC CCGTACCAGAGGAGACTGTGAAAAAGATCCTTGGAAACAAAGCAACGTTTAGCCCAATTGTCACGGTGGA GCCGAGGAGGAGGAAATTCCATAAGCCGATCACCATGACCATCCCGGTGCCCCCACCCTCGGGAGAAGGC GTGTCCAATGGGTACAAGGGGGACACCACGCCCAGCCTGCGACTCCTCTGCAGCATTACAGGAGGGACCT CGCCGGCTCAGTGGGAAGACATCACAGGCACAACCCCGCTGACCTTCATAAAGGACTGTGTGTCTTTCAC AACCAATGTTTCAGCCAGGTTCTGGCTGGCGGACTGCCATCAGGTGTTAGAAACCGTAGGGCTAGCCTCG CAGCTGTACAGAGAGTTGATATGTGTCCCCTACATGGCCAAGTTTGTTGTTTTTGCCAAAACAAACGACC CAGTGGAATCCTCCCTAAGGTGCTTCTGTATGACAGACGACAGAGTGGACAAAACCCTGGAGCAGCAGGA GAATTTCGAGGAGGTTGCCAGAAGCAAAGACATCGAGGTTCTGGAAGGAAAGCCCATCTATGTTGATTGC TATGGAAACCTGGCTCCTCTGACCAAAGGAGGGCAGCAGCTTGTTTTTAACTTCTATTCTTTCAAAGAAA ACAGACTGCCATTTTCCATCAAGGTTAGAGACACCAGCCAAGAGCCCTGCGGCCGTCTGTCTTTCCTGAA GGAGCCGAAAACAACAAAAGGATTACCCCAAACGGCTGTTTGCAACTTAAATATTACTCTACCGGCGCAT AAAAAGGCTGAGAAGGCAGACAGACGCCAGAGCTTCACATCCCTAGCCTTACGTCCACAGAGTCCTTGTG AGCGGACGGATATCAGGATGGCAATAGTAGCCGATCACCTGGGACTTAGTTGGACAGAGCTGGCAAGGGA ACTGAATTTTTCAGTGGATGAAATCAACCAAATACGAGTGGAAAATCCCAATTCTTTAATTTCTCAGAGC TTCATGTTATTAAAAAAGTGGGTGACGAGAGACGGGAAGAATGCCACAACTGATGCCTTAACTTCGGTCT TAACAAAAATTAACCGAATAGACATAGTAACTCTGCTGGAAGGACCAATATTTGATTATGGGAATATTTC AGGCACCAGAAGCTTTGCAGATGAAAACAATGTTTTCCATGACCCAGTTGATGGTCACCCTTCCTTTCAA GTGGAGCTGGAGACCCCAATGGGGTTGTACTGCACACCACCCACCCCTTTCCAGCAAGATGACCATTTTA GTGATAACTCTAGCATAGAGTCTCCCTTTAGGACCCCCAGTAGACTGAGTGACGGGCTGATGCCTTCCCA GGGAAGCATAGAGCATCCAGCAGGTGGACCTCCAGTGGTAACTGCAGAAGACACTTCTTTAGAAGACAGC AAAATGGACGATTCAGTAACGGTAACAGAAACGGCTGACCCACTGGACGTAGATGAGAGCCAGTTGAAGG ACCTGTGTCAGAGCGAGTGTGCTCACTGCTGGGCGAGTGTGCCCGGGGTCCCAAGCGGCGGTCCGCAGGC AGAGCCACTGAGAGCGCAGACTAGAAAAGTAGGCGTGAGCTCTGAACAGCAGGAGAAAGGAGACTCTGGT CCTGAGGAGGAGATGGCGGACGACAAGGTCAGATCTCTGTTTGAGGACATTCAACTTGAAGAAGTAGAGG CTGAGGAGATGACAGAAGACGAGGGGCAGGCTATACTTAACCGTGTTCCGCGAGCAGAACTGGCAATGTC TTCACTTGCAGGTTGGCAGAACGAGACGCCAAGTGGAAGCCTAGAGTCCCCGGCGCAAGCGCGAAGAATA ACCGGTGGGTTACTGGACCGTCTGGACGACAGCTCTGACCAGGTTCGGGATCCCATTACCTCATACCTCA CAGGAGAAGCTGGGAAGTTCGAAGCAAACGGAAACCACGCAGAAGTCATTCCAGAAGCAAAGGCCAAGGC CTACTTTCCTGAATCCCAAAACGATATAGGGAAACAGAGCATCAAGGAGAACTTGAAACCAAAAACACAC GGATGTGGTCGCGCTGAGGAACCAGTGTCGCCCCTCACAGCCTACCAGAAATCTCTAGAAGAAACCAGCA AGCTTGTCATAGAAGACGCACCTAAACCCTGTGTGCCTGTCGGCATGAAAAAGATGACCAGGACTCCGGC TGACGGCAAAGCCAGGCTCAACCTCCAGGAAGAGGAGGGGTCCGCCAGGTCAGAGCCTAAGGACTTGAGA CACAGTGAGAGTGATTCAAGCTCAGAGGAGGAGCGGAGGGTCACTACCCGGGTCATACGACGGCGTGTGA TTATAAAGGGAGAGGAAGCCAAAACCATCCCTGGTGAATCTGTCACAGAAGAGCAATTTACCGATGAAGA AGGCAACCTCATCACTAGAAAAATCACTCGGAAGGTAATAAGACGGATTGGTCCACAAGAGAGGAAGCAG GATGATGTGCAGGGAGAAGGCTATAAGGTGAAAACAAAGAAGGAAATCCGGAATGTGGAGAAGAAAGCCC AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200065 representing NM_031805 Red=Cloning site Green=Tags(s) MSEEAKEKTAKPAHRKRKGKKSDANASYLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHV EVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVR FLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDTN ADIESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAK TRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDY LTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPI HVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISA RLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGK LEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIA TSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLV NQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNA SPNELTVNGNTALAIARRLGYISVVDTLKVVTEEIMTTTTITEKHKMNVPETMNEVLDMSDDEGEDAITG DTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASPKISSDRSYTLNRSSYARDSMMIEELLVPSKEQHL PFTREFDSDSLRHYSWAADTLDNVNLVSSPVHSGFLVSFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTR ITCRLVKRHKLANPPPMVEGEGLASRLVEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWK EHQFDSKNEDLSELLNGMDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTV PLVQASFPEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPSGEG VSNGYKGDTTPSLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLADCHQVLETVGLAS QLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQENFEEVARSKDIEVLEGKPIYVDC YGNLAPLTKGGQQLVFNFYSFKENRLPFSIKVRDTSQEPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAH KKAEKADRRQSFTSLALRPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQS FMLLKKWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDGHPSFQ VELETPMGLYCTPPTPFQQDDHFSDNSSIESPFRTPSRLSDGLMPSQGSIEHPAGGPPVVTAEDTSLEDS KMDDSVTVTETADPLDVDESQLKDLCQSECAHCWASVPGVPSGGPQAEPLRAQTRKVGVSSEQQEKGDSG PEEEMADDKVRSLFEDIQLEEVEAEEMTEDEGQAILNRVPRAELAMSSLAGWQNETPSGSLESPAQARRI TGGLLDRLDDSSDQVRDPITSYLTGEAGKFEANGNHAEVIPEAKAKAYFPESQNDIGKQSIKENLKPKTH GCGRAEEPVSPLTAYQKSLEETSKLVIEDAPKPCVPVGMKKMTRTPADGKARLNLQEEEGSARSEPKDLR HSESDSSSEEERRVTTRVIRRRVIIKGEEAKTIPGESVTEEQFTDEEGNLITRKITRKVIRRIGPQERKQ DDVQGEGYKVKTKKEIRNVEKKAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031805
ORF Size	5952 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031805.1, NP_113993.1
RefSeq Size	7401 bp
RefSeq ORF	5955 bp
Locus ID	361833
UniProt ID	O70511
MW	216.6 kDa
Gene Summary	may play a role in the organization of the sarcoplasmic reticulum and postsynaptic membrane of skeletal muscle fibers [RGD, Feb 2006]

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