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Gmcl1 (NM_001033931) Rat Tagged ORF Clone
CAT＃: RR200084
- TrueORF®
Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 1, (10 ug)
"NM_001033931" in other vectors (3)
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CNY 6,750.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gcl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200084 representing NM_001033931
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCTCTGAGCAGCCGGGTCCTGCGGCCCGCGGGGCGCACAGAGCAGCCCGAACCCACGCCCGGTG CTGGGGGCTCGGCCCGCAGATCGGACGCGGGCGAAGATGCGGCCCACGGCTTCTGTTACTGTCCCGGCGG CCGCAAGCGCAAGCGCAGCAGCGGCACTTTCTGCTACTGTCACCCCGAGTCAGAGACAGACGAAGACGAG GACGAGGGCGACGAGCAGCAGAGGCTGCTGAACACGCCGCGCAGGAAAAAATTAAAGAGCACATCGAAAT ACATCTACCAAACGCTGTTTTTGAATGGTGAAAACAGTGACATTAAGATCTGTGCTCTAGGTGAAGAGTG GAGCTTACACAAAATCTACTTATGTCAATCTGGCTACTTTTCTAGTATGTTCAGTGGTTCTTGGAAAGAA TCCAGCATGAATATTATTGAACTGGAGATTCCTGACCAGAACATTGATATAGAAGCACTGCAGGTTGCAT TTGGATCACTGTATCGAGATGACGTCTTAATAAAGCCCAGCAGGGTTGTCGCCATTTTGGCAGCAGCTTG CATGCTGCAGTTGGATGGTTTGATACAGCAGTGTGGTGAGACCATGAAGGAGACCATCTCTGTAAGAACT GTGTGTGGCTATTACACATCGGCAGGGACCTATGGACTAGACTCTGTAAAGAAAAAGTGCCTCGAGTGGC TGCTGAACAACCTCATGACTCACCAGAGTGTGGAGCTTTTCAAAGAACTCAGTATAAACGTCATGAGGCA GCTCATTGGTTCATCTAACTTATTTGTGATGCAAGTGGAAATGGATGTATATACAGCTCTGAAAAAGTGG ATGTTCCTTCAGTTGGTGCCTTCCTGGAATGGGTCTTTAAAGCAGCTTTTGACAGAAACAGATGTCTGGT TTTCAAAGTGGAAAAAAGACTTTGAAGGGACGACTTTCCTTGAAACTGAGCAGGGAAAACCATTTGCGCC CGTGTTCAGACATTTAAGGCTACAGTACATTATCAGTGATCTGGCTTCTGCAAGGATCATTGAGCAGGAC TCTCTGGTACCTTCAGAATGGCTGGCTTCAGTGTATAAGCAGCAGTGGTTGGCTATGCTTCGGGCTGAAC AAGACAGTGAAGTGGGGCCTCAAGAAATCAATAAAGAAGAACTTGAGGGAAATAGCATGAGGTGTGGTAG AAAGCTTGCCAAAGATGGTGAATACTGCTGGCGGTGGACAGGTTTTAATTTTGGCTTTGACCTCCTTGTG ACTTACACCAATCGATACATCATTTTCAAACGCAATACGCTAAACCAGCCATGTAGTGGATCTGTCAGCT TGCAGCCTCGAAGAAGCATAGCATTTAGATTGCGCTTGGCTTCTTTCGACAGTAGTGGAAAACTCATATG CAGTAGAGCGACTGGCTACCAAATCCTGACGCTTGAGAAAGACCAGGAGCAAGTGGTGATGAACTTGGAC AGCAGACTGCTGATCTTCCCTCTATACATCTGCTGTAACTTCTTGTATATATCACCAGAAAAAAGAACTG AGAGTAATCGTCACCCAGAAAACCCAGGACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200084 representing NM_001033931
Red=Cloning site Green=Tags(s) MGALSSRVLRPAGRTEQPEPTPGAGGSARRSDAGEDAAHGFCYCPGGRKRKRSSGTFCYCHPESETDEDE DEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKE SSMNIIELEIPDQNIDIEALQVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRT VCGYYTSAGTYGLDSVKKKCLEWLLNNLMTHQSVELFKELSINVMRQLIGSSNLFVMQVEMDVYTALKKW MFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRLQYIISDLASARIIEQD SLVPSEWLASVYKQQWLAMLRAEQDSEVGPQEINKEELEGNSMRCGRKLAKDGEYCWRWTGFNFGFDLLV TYTNRYIIFKRNTLNQPCSGSVSLQPRRSIAFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLD SRLLIFPLYICCNFLYISPEKRTESNRHPENPGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033931 |
| ORF Size | 1572 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033931.2, NP_001029103.1 |
| RefSeq Size | 2947 bp |
| RefSeq ORF | 1575 bp |
| Locus ID | 312516 |
| MW | 59.7 kDa |
| Gene Summary | This gene encodes a nuclear envelope protein that contains a broad-complex, tramtrack and bric a brac/ Pox virus and Zinc finger (BTB/POZ) protein-protein interaction domain. In mouse, this gene is expressed at high levels in the testis, and null mutant mice exhibit morphologically abnormal sperm that have chromatin condensation defects. These defects result in significantly impaired fertility, demonstrating a role for this gene in male germ cell development in mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200084 | Gmcl1 (untagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 1, (10 ug) |
CNY 6,750.00 |
|
| RR200084L3 | Lenti ORF clone of Gmcl1 (Myc-DDK-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 1, (10 ug) |
CNY 8,650.00 |
|
| RR200084L4 | Lenti ORF clone of Gmcl1 (mGFP-tagged ORF) - Rat germ cell-less homolog 1 (Drosophila) (Gmcl1), transcript variant 1, (10 ug) |
CNY 9,500.00 |
