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Rtn3 (NM_001009953) Rat Tagged ORF Clone
CAT＃: RR200210
- TrueORF®
Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 2, (10 ug)
"NM_001009953" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200210 representing NM_001009953
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGTCATCGGCGGCCACTCAGTCTCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAA CGCTTGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCCTG TGCGGTGCATGATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACCACGCTGATC ATGCTGCTCTCTCTGGCAGCTTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTCTACTCTCTG TCACCATCAGCTTCAGAGTCTACAAGTCTGTCATCCAAGCTGTGCAGAAGTCAGAAGAAGGACATCCATT CAAGGCCTACCTGGATGTGGACATTACACTGTCCTCAGAAGCTTTCCACAGCTACATGAATGCTGCAATG GTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTGACTCCTTGA AGCTGGCTGTCTTCATGTGGCTGATGACCTACGTCGGTGCTGTTTTTAACGGAATTACCCTTCTGATTCT CGCCGAGCTGCTGGTTTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGACCACTATGTT GGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAATCGCCAAAA AAAAGGCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200210 representing NM_001009953
Red=Cloning site Green=Tags(s) MAESSAATQSPSVSSSSSGAEPSTLGGGGGSPGACPALGAKSCGSSCAVHDLIFWRDVKKTGFVFGTTLI MLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHSYMNAAM VHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQIDHYV GIARDQTKSIVEKIQAKLPGIAKKKAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001009953 |
| ORF Size | 711 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001009953.3, NP_001009953.2 |
| RefSeq Size | 2817 bp |
| RefSeq ORF | 714 bp |
| Locus ID | 140945 |
| UniProt ID | Q6RJR6 |
| MW | 25.4 kDa |
| Gene Summary | This gene encodes a member of the reticulon family, a large group of membrane-bound proteins that primarily localize to the endoplasmic reticulum with a small subpopulation at the cell surface. These proteins play roles in endocytosis, exocytosis, trafficking and neuronal outgrowth. Proteins belonging to this family are characterized by a carboxy-terminal reticulon homology domain that consists of a small hydrophilic loop flanked by hydrophobic regions. In rat, this protein has been shown to interact with two members of the synaptic adhesion-like molecule family, suggesting that it may function in trafficking of these proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200210 | Rtn3 (untagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 2, (10 ug) |
CNY 3,990.00 |
|
| RR200210L3 | Lenti ORF clone of Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 2, (10 ug) |
CNY 6,080.00 |
|
| RR200210L4 | Lenti ORF clone of Rtn3 (mGFP-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 2, (10 ug) |
CNY 6,650.00 |
