Gnas (NM_001024823) Rat Tagged ORF Clone
CAT#: RR200464
- TrueORF®
Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug)
"NM_001024823" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200464 representing NM_001024823
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCCCCTGAAGACTCCAAATCTGACATCCA GAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACTACAGCCCACCTCCAGAGGAAGCCATG CCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCACCCTGGAGCAGCCTGGACCATCTGGGA CCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCCGGGACCCCCACTGGTGCACACCCAGG CCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTGAGTTCAATGAGCCTGCCCAGGAAGAC CGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGGTCCGGAAGCATGGGTCTCCAGAGCTC TTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTCCGAGAAGACTACAGCCCTCCACCTGA AGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCGATAGCCCACCCCCAGGACTCCCGCGA GTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGCGGTCCCGAGTACGCTCTGCCTCGCTC CCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGCAGACCATTCCGCGAGGCTGTCAGATC TCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGACCCCTGCTTGAGATTGGCAGAGCCTCC ACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCCAATCGCAAGTGATGGCCCGCCCATCG AAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCCCCACTTGAGCGACAAGCAGCCGAGAC AGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAGTCCCCTCCCTCGAGCGAGGAGAAGGA TCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGTTGCGGAGTCCGGAACGGACTCTTCCA AAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGTCCTGAGGAAGTCGGAGGAGTCCCAAC CATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAGATGTCCGGGCAGAACCAGACGGAGGG ACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACCAGCCGCCGCCGCCGCCGCCGAGCCAG CCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCCGCCGAGCCAGCTGCCGAGGC AGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCGACACCCAAGAGGAGCCCGCAGCCGCG GCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCCCGTCACCCCTACGGAGCCCGCTACCC GGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTCCCTGGAGCCTCAGCGATGTCAGCCGC TGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGGTCTGGGGAGCCAGGTCACTCTCGGCT ACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGCCGCCCGAGCAGCCTCTGCTGCCCGCG CAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCCCATCTTAGACCCCCCAGCCCGGAGAT CCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGAGTGCCTGGCCTGACAAATACGAACGT GGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGAGATCGAGTCCTCCAGCGATGAGTCGG AAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAACCGCCGCCCTGGCCAGCCCCGGAGCCA CACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCTTCGGAAGCTGCTTCGGTCTATCTGAG TGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCCTATGGAAGAGAGGCGCAAACAGATGC GCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAGAAACGCAGCAAGCTCATCGACAAGCA ACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGCTGCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200464 representing NM_001024823
Red=Cloning site Green=Tags(s) MSPSPTRLTVRSVDPLKTPNLTSRAPVRPSRKSKWAETTAHLQRKPCHSRSNSPAWEISGPPWSSLDHLG PHQASKPSTQRFWSPGPPLVHTQAWEPIAPHQKKLCHLSSMSLPRKTVASLPCKSQTLRQGVRKHGSPEL FPRSPGTSDLKTLASEKTTALHLKNLCHFSSMEKNSGAIAHPQDSRESRHKSALAASSRQSRSRVRSASL PPRTRLPSGSRAPLADHSARLSDLLTSHTTFPQWRSPDPCLRLAEPPLGSTTTPLSIWTAPQSQVMARPS KSREPQLRASTQRDPHLSDKQPRQETALSAAPLQRRQKSPPSSEEKDPPPNLKQCISSQLLLRSPERTLP KPIRTQLHTQFFRSVLRKSEESQPCPPIFRLLLKMRAQMSGQNQTEGQPQPPLPSPKTTENQPPPPPPSQ PPSQPLSQPPSQPPSQPPSQLPRQSLTPKPSLPPGQSPTPKRSPQPRQPLPRRRSLPPGQPPSPLRSPLP GLSLLPEPIQPPGLSLEPQRCQPLLGQPPLEQPMQVLWSGEPGHSRLLQPLGHPSLPAQQLPPEQPLLPA QSLPAGQPLPPQAGPILDPPARRSRLLTRLLRGLLRGRVPGLTNTNVAEAAAGMRLRPASARSSPPAMSR KKGPLAASSGFCGETAALASPGATQSGATRSATSSPEPSEAASVYLSVPDHDPSAPGRPRILWKRGANRC AKKPWRCESRSAQIRNAASSSTSNWRRRRWTTCVHTACCF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_001024823 |
ORF Size | 2220 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024823.4, NP_001019994.4 |
RefSeq Size | 3826 bp |
RefSeq ORF | 2223 bp |
Locus ID | 24896 |
MW | 80.7 kDa |
Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200464 | Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
CNY 3,990.00 |
|
RR200464L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
CNY 6,080.00 |
|
RR200464L4 | Lenti ORF clone of Gnas (mGFP-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 2, (10 ug) |
CNY 6,650.00 |