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Bcar3 (NM_001107722) Rat Tagged ORF Clone
CAT＃: RR200632
- TrueORF®
Bcar3 (Myc-DDK-tagged ORF) - Rat breast cancer anti-estrogen resistance 3 (Bcar3), (10 ug)
"NM_001107722" in other vectors (3)
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CNY 10,550.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200632 representing NM_001107722
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGGAAAATTTGCAAGCCTTCCCAGACACATGCCCGTGAATCACCAGTTCCCTTTGGCCTCGT CCATGGACCTCCTGAGCAGCAAGTCCCCTCTCGCTGAGCATCGCACAGAGGCCTATCCAGACGTGTCCAT ACACGGCACTCTTCCACGGAAGAAAAAAGGCCCTCCTCCCATACGGTCCTGTGACAGTGCCAGTCACATG GGCACCCTCCCTCACTCCAAGTCCCCACGACAGAGCTCACCTCTGACCCAGGACCTCATCCTGGAAAAAC CACTGCCAGACTGGAAAGGGGACAGCTTCGCCTTCAGGGATCCCTATCTTCTGGACCCAACTCTGGAGTA CGTGAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCTGAGAGGCTGAAAAAGGAACTGGAGGAG GAGCTACTGCTGAGCAGCGAGGACCTACGCAGCCATGCCTGGTACCACGGCCGGATCCCCAGACAGGTGT CTGAAAACCTTGTGCAGCGGGATGGGGACTTCCTGGTTCGTGACTCCCTGTCTAGCCCTGGAAACTTTGT CCTGACCTGTCAGTGGAAGAACCTTGCTCAGCACTTTAAGATCAACCGGACTGTTCTGCGGCTCAGCGAG GCCTACAGCCGTGTGCAGTACCAATTCGAGATGGAGAGCTTTGACTCCATCCCCGGGCTGGTACGCTGCT ACGTGGGAAACCGGAGGCCCATCTCCCAGCAGAGCGGCGCCATCATCTTCCAGCCTATCAATAGGACAGT GCCCCTTTGGTGTCTGGAGGAGCGTTATGGCACCTCACCAGGCCGAGGCCGGGAGGGCAGCTTTGCTGAG GGAAGACCAGATGTGGTGAAGAGACTGAGCCTCACCACAGGTGGCATCCAGGCTCGGGACCACAGCTTGC CCAGAGGAAACCTCCTCAGGAATAAGGACAAGAGTGGCAGCCAGCCCGCCTGCCTGGATCACGTGCAGGA CCGGAAGGCCGCAACACTCAAGGCTCACCAGTCTGAGAGTCACCTGCCCATCGGCTGCAAGCTGCCACCC CAGTCTCCGAGTGTGGACACAAGCCCTTGCCCCAACTCTCCCGTGTTCAGGACTGGCAGCGAGCCCACTC TGAGTCCGGCACTGGTACGAAGGTTCTCTTCGGACGCTAGGGCAGGGGAGGCACTTCGGGGATCAGACAG TCAACTGTGCCCCAAGCCACCCCCAAAGCCCTGCAAGGTGCCCTTCCTCAAGGTTCCTCCCTCTCCATCT CCCTGGCTAAACTCAGAGGCCAACTACTGTGAACTGAACCCTGCTTTTGCGGTGGGCTGTGACAGGGGAG CCAAGCTTCTCTCACAGGCTCTCGACAGCCACGAGATGCTGCTGACAGCCAAACAGAATGGGGCATCGGG TCCCCGGAACTCTGGCATCAACTACTCGATACTTGATGGGGATGACCAAGGGAGACATTGGGATCCCCTG GCCGTGCAGACGGATGAGGGTCAGGAGGACGAGACCAAGTTTGTACCACCTGTCATGGAGACAGTGTCAT CATTCAGACCCAATGACTTTGAGTCGAAGCTTCTTCCTCCAGAGAACAAGCCCCTGGAAACAGCCATGCT AAAGCATGCCAAGGAACTGTTCACCAACCATGACGCGAGGGTCATTGCACAGCACATGCTGAGCGTGGAC TGCAAGGTTGCTAGGATACTGGAAGTCTCTGAAGACATGAAGAGGAGCATGGGCGTGAGCTCGGGACTGG AACTCATTACCTTGCCTCACGGACGCCAGCTGCGCCTGGACATTATTGAAAGACACAACACCATGGCCAT TGGCATTGCTGTGGACATTCTGGGCTGCACGGGCACATTGGAGAACCGGGCAGGAACCCTTAATAAGATC ATCCAGGTGGCAATGGAACTGAAGGACACCATGGGAGACCTCTATTCCTTCTCAGCCATCATGAAAGCCC TCGAGATGCCTCAGATCACAAGGTTAGAAAAAACATGGACGGCTCTGAGGCACCACTACACGCAAACAGC CATCCTCTACGAAAAGCAGTTGAAGCCCTTTAGCAAAATCCTGCATGAAGGCAGAGAGTCGACATATGTC CCAGCAAGCAGTGTGTCCGTCCCACTGCTGATGCCTCTTGTGACCTTAATGGAACGGCAGGCTGTCACTT TTGAAGGAACCGACATGTGGGAAAAAAATGACGAGAGCTGTGAAATCATGCTGAGTCACTTGGCCACAGC CAGGTTCATGGCCGAGGCTTCGGAGAGCTACAGGATGAATGCTGAGAGGGTCCTGGCAGATTTCCAACCG GATGAAGAAATGACTGAAATCCTAAAGACTGAGTTCCAGATGCGATTGTTGTGGGGCAGCAAGGGTGCTG AAGTCAACCAGAACGAGAGATATGACAAGTTCAACCAGATCCTAACTGCCCTCTCACGGAAACTGGAACC TCCTTCTGGAAAGCAGGCTGAGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200632 representing NM_001107722
Red=Cloning site Green=Tags(s) MAAGKFASLPRHMPVNHQFPLASSMDLLSSKSPLAEHRTEAYPDVSIHGTLPRKKKGPPPIRSCDSASHM GTLPHSKSPRQSSPLTQDLILEKPLPDWKGDSFAFRDPYLLDPTLEYVKFSKERHIMDRTPERLKKELEE ELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSE AYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLWCLEERYGTSPGRGREGSFAE GRPDVVKRLSLTTGGIQARDHSLPRGNLLRNKDKSGSQPACLDHVQDRKAATLKAHQSESHLPIGCKLPP QSPSVDTSPCPNSPVFRTGSEPTLSPALVRRFSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPPSPS PWLNSEANYCELNPAFAVGCDRGAKLLSQALDSHEMLLTAKQNGASGPRNSGINYSILDGDDQGRHWDPL AVQTDEGQEDETKFVPPVMETVSSFRPNDFESKLLPPENKPLETAMLKHAKELFTNHDARVIAQHMLSVD CKVARILEVSEDMKRSMGVSSGLELITLPHGRQLRLDIIERHNTMAIGIAVDILGCTGTLENRAGTLNKI IQVAMELKDTMGDLYSFSAIMKALEMPQITRLEKTWTALRHHYTQTAILYEKQLKPFSKILHEGRESTYV PASSVSVPLLMPLVTLMERQAVTFEGTDMWEKNDESCEIMLSHLATARFMAEASESYRMNAERVLADFQP DEEMTEILKTEFQMRLLWGSKGAEVNQNERYDKFNQILTALSRKLEPPSGKQAEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107722 |
| ORF Size | 2475 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107722.1, NP_001101192.1 |
| RefSeq Size | 3337 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 310838 |
| UniProt ID | D3ZAZ5 |
| MW | 92.5 kDa |
| Gene Summary | Acts as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (By similarity). Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed:24216110). Promotes insulin-mediated membrane ruffling (PubMed:24216110). In response to vasoconstrictor peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (By similarity). Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity). May inhibit TGFB/SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity). Regulates EGF-induced DNA synthesis (By similarity). Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity). Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity). Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity). However, in a contrasting study, lacks GEF activity towards RAP1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200632 | Bcar3 (untagged ORF) - Rat breast cancer anti-estrogen resistance 3 (Bcar3), (10 ug) |
CNY 10,550.00 |
|
| RR200632L3 | Lenti ORF clone of Bcar3 (Myc-DDK-tagged ORF) - Rat breast cancer anti-estrogen resistance 3 (Bcar3), (10 ug) |
CNY 12,350.00 |
|
| RR200632L4 | Lenti ORF clone of Bcar3 (mGFP-tagged ORF) - Rat breast cancer anti-estrogen resistance 3 (Bcar3), (10 ug) |
CNY 13,590.00 |
