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Nlgn1 (NM_053868) Rat Tagged ORF Clone
CAT＃: RR200719
- TrueORF®
Nlgn1 (Myc-DDK-tagged ORF) - Rat neuroligin 1 (Nlgn1), (10 ug)
"NM_053868" in other vectors (3)
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CNY 10,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200719 representing NM_053868
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTTCCCAGATGCATGTGGCCAAATTATGTTTGGAGAGCCATGATGGCATGTGTGGTCCACAGGG GATCGGGTGCCCCGTTGACTCTCTGCTTGTTGGGATGTTTGCTACAGACTTTCCACGTACTCTCTCAAAA GTTGGATGATGTAGACCCATTGGTTACTACTAACTTTGGCAAAATAAGGGGAATTAAGAAAGAACTCAAT AATGAAATTTTGGGGCCTGTCATTCAGTTTCTTGGGGTTCCATATGCTGCTCCACCAACAGGAGAACATC GATTTCAGCCTCCAGAACCACCATCTCCCTGGTCTGATATCCGGAATGCCACTCAGTTTGCTCCTGTATG TCCCCAGAATATCATTGATGGCAGATTGCCTGAAGTTATGCTTCCTGTGTGGTTCACTAATAACTTGGAT GTGGTTTCCTCATATGTGCAAGACCAGAGTGAAGACTGTCTATACTTAAACATCTATGTCCCAACTGAAG ATGTAAAAAGAATATCCAAGGAATGTGCCAGAAAACCCGGCAAGAAAATATGTAGAAAAGGAGATATTCG GGACAGTGGGGGTCCCAAACCAGTGATGGTGTACATCCATGGTGGCTCTTACATGGAAGGTACTGGAAAT CTATATGATGGGAGTGTGTTGGCAAGCTACGGTAATGTGATCGTCATCACAGTCAACTATCGGCTTGGGG TACTTGGCTTCTTGAGCACAGGGGATCAGGCTGCCAAAGGAAACTACGGGCTCCTTGACCTCATCCAGGC CCTCAGATGGACCAGCGAGAACATTGGGTTCTTTGGTGGTGACCCCTTGCGAATCACCGTGTTTGGATCA GGCGCTGGGGGTTCATGTGTCAATCTGCTGACTTTATCCCATTATTCTGAAGGTAACCGTTGGAGCAATT CAACCAAAGGACTTTTTCAACGAGCAATAGCTCAGAGTGGAACAGCCCTTTCCAGCTGGGCTGTTAGTTT CCAGCCTGCAAAATACGCTAGAATTCTAGCCACAAAAGTTGGCTGCAATGTGTCAGATACAGTAGAGTTA GTAGAATGCCTGCAGAAGAAGCCTTACAAAGAACTTGTTGATCAAGATGTTCAACCAGCCCGATACCACA TAGCCTTTGGACCTGTGATCGATGGTGATGTAATACCAGATGACCCTCAGATACTGATGGAACAAGGAGA GTTCCTCAATTATGATATCATGTTAGGAGTTAACCAAGGGGAAGGGTTGAAGTTTGTGGAAAACATAGTA GATAGTGATGATGGTGTATCAGCCAGTGATTTTGACTTTGCTGTCTCTAATTTTGTTGATAATTTATATG GATATCCTGAAGGCAAAGATGTTTTGAGAGAAACCATTAAATTCATGTATACTGACTGGGCTGATCGCCA TAATCCTGAAACTAGAAGGAAGACATTGTTGGCTTTGTTTACAGACCATCAGTGGGTAGCACCCGCTGTG GCTACAGCAGACCTTCACTCGAACTTTGGCTCACCTACATACTTCTATGCCTTTTATCATCATTGCCAAA CAGACCAAGTTCCAGCTTGGGCTGATGCAGCTCATGGGGATGAGGTTCCCTATGTATTGGGAATCCCCAT GATTGGCCCTACAGAGTTATTTCCTTGCAATTTCTCCAAGAATGATGTGATGTTGAGTGCTGTAGTAATG ACATACTGGACGAATTTTGCTAAAACCGGTGACCCAAATCAACCAGTTCCTCAAGACACAAAATTCATTC ATACCAAACCCAACCGCTTTGAAGAAGTAGCATGGACCAGATATTCCCAGAAAGACCAACTTTATCTCCA TATTGGATTAAAACCGAGAGTTAAAGAACATTACAGAGCCAATAAGGTAAATCTCTGGTTGGAGCTGGTA CCTCATCTGCATAATCTCAATGACATTTCTCAGTATACCTCGACAACAACTAAAGTGCCATCAACAGACA TCACTCTCAGACCTACAAGGAAAAATTCCACTCCAGTCACATCAGCCTTTCCCACTGCCAAGCAGGATGA TCCCAAACAACAACCAAGTCCCTTCTCGGTAGATCAAAGGGACTACTCCACAGAGCTAAGTGTCACAATC GCAGTGGGAGCCTCTCTGCTGTTTCTGAACATCTTGGCTTTTGCAGCCCTATACTACAAGAAAGACAAGA GGAGACATGATGTCCACCGGAGGTGCAGCCCTCAGCGCACCACGACCAACGACCTAACCCATGCTCCAGA AGAGGAAATTATGTCTCTCCAAATGAAGCACACTGACTTGGATCACGAGTGTGAGTCCATCCATCCACAT GAGGTGGTTCTTCGGACCGCCTGCCCCCCAGATTATACTCTTGCTATGAGGAGGTCACCGGATGATGTTC CTTTAATGACACCTAACACCATCACAATGATTCCCAACACTATACCAGGGATTCAGCCCTTACATACATT CAACACATTTACTGGAGGACAGAATAATACACTGCCCCACCCCCACCCTCACCCCCATTCACATTCAACA ACCAGGGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200719 representing NM_053868
Red=Cloning site Green=Tags(s) MALPRCMWPNYVWRAMMACVVHRGSGAPLTLCLLGCLLQTFHVLSQKLDDVDPLVTTNFGKIRGIKKELN NEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLD VVSSYVQDQSEDCLYLNIYVPTEDVKRISKECARKPGKKICRKGDIRDSGGPKPVMVYIHGGSYMEGTGN LYDGSVLASYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGS GAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVEL VECLQKKPYKELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENIV DSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAV ATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVM TYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELV PHLHNLNDISQYTSTTTKVPSTDITLRPTRKNSTPVTSAFPTAKQDDPKQQPSPFSVDQRDYSTELSVTI AVGASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTTNDLTHAPEEEIMSLQMKHTDLDHECESIHPH EVVLRTACPPDYTLAMRRSPDDVPLMTPNTITMIPNTIPGIQPLHTFNTFTGGQNNTLPHPHPHPHSHST TRV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053868 |
| ORF Size | 2529 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053868.2, NP_446320.1 |
| RefSeq Size | 3322 bp |
| RefSeq ORF | 2532 bp |
| Locus ID | 116647 |
| UniProt ID | Q62765 |
| MW | 94.3 kDa |
| Gene Summary | transmembrane ligand for neurexins; involved in development and maturation of synaptic connections [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200719 | Nlgn1 (untagged ORF) - Rat neuroligin 1 (Nlgn1), (10 ug) |
CNY 10,740.00 |
|
| RR200719L3 | Lenti ORF clone of Nlgn1 (Myc-DDK-tagged ORF) - Rat neuroligin 1 (Nlgn1), (10 ug) |
CNY 12,640.00 |
|
| RR200719L4 | Lenti ORF clone of Nlgn1 (mGFP-tagged ORF) - Rat neuroligin 1 (Nlgn1), (10 ug) |
CNY 13,780.00 |
