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Manf (NM_001108183) Rat Tagged ORF Clone
CAT＃: RR201485
- TrueORF®
Manf (Myc-DDK-tagged ORF) - Rat arginine-rich, mutated in early stage tumors (Armet), (10 ug)
"NM_001108183" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Armet |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR201485 representing NM_001108183
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCTACGCGCGGGCTGGCGGTAGCGCTGGCCCTGAGCGTTCTGCCTGACAGTCGGGCGCTACGGC CAGGCGACTGCGAAGTTTGTATTTCTTATCTGGGGAGATTTTACCAGGACCTCAAAGACAGGGATGTCAC ATTTTCACCAGCCACTATTGAAGAAGAACTTATAAAGTTCTGCCGGGAAGCAAGAGGCAAAGAAAATCGG TTGTGCTACTACATTGGAGCCACAGATGATGCCGCCACCAAGATCATCAATGAGGTATCGAAGCCTCTGG CCCACCATATCCCTGTGGAGAAGATCTGTGAGAAGCTGAAGAAGAAAGACAGCCAGATCTGCGAGCTAAA ATACGGTGAGTGTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201485 representing NM_001108183
Red=Cloning site Green=Tags(s) MWATRGLAVALALSVLPDSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIEEELIKFCREARGKENR LCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYGECD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108183 |
| ORF Size | 366 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108183.1, NP_001101653.1 |
| RefSeq Size | 550 bp |
| RefSeq ORF | 369 bp |
| Locus ID | 315989 |
| MW | 13.8 kDa |
| Gene Summary | Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain (By similarity). Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (PubMed:16462600). Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (PubMed:16462600). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death (By similarity). Retained in the ER/sarcoplasmic reticulum (SR) through association with the endoplasmic reticulum chaperone protein HSPA5 under normal conditions (By similarity). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (By similarity). Following secretion by the ER/SR, directly binds to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells (By similarity). Sulfatide binding promotes its cellular uptake by endocytosis, and is required for its role in alleviating ER stress and cell toxicity under hypoxic and ER stress conditions (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN201485 | Manf (untagged ORF) - Rat arginine-rich, mutated in early stage tumors (Armet), (10 ug) |
CNY 3,990.00 |
|
| RR201485L3 | Lenti ORF clone of Manf (Myc-DDK-tagged ORF) - Rat arginine-rich, mutated in early stage tumors (Armet), (10 ug) |
CNY 6,080.00 |
|
| RR201485L4 | Lenti ORF clone of Manf (mGFP-tagged ORF) - Rat arginine-rich, mutated in early stage tumors (Armet), (10 ug) |
CNY 6,650.00 |
