Scn7a (NM_031686) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Na-G; Scn6a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201560 representing NM_031686 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGACTTCCCCAGAGCCGAAGGGCCTTGTCCCATTCACGGCAGAGTCACTTGAACTTATAAAAAATC ACATTGCTAAAAAATGCAACGAAGAGCATGAAGAAGAAGATTTAAAACCAAGCCGGGATATAGAAGCAGG CAAAAAACTTCCATTTGCCTATGGAACCCTTCCTCAAGGAACCGTGTCAGAGCCCTTGGAAGATGTGGAT CCATACTACTATGTTAAGAGAAATACTTTCATGGTCTTAAACAGAAACAGAGTCATCTTCAGGTTCAATG CGGTTTCCATCCTCTGCACGTTGTCTCCTTTAAGCTCTCTCAGAAGAGCTGTTATCAAGGTTTTGGTGCA CCCCCTTTTGCGCCTGCTGATTTTAATTAGTGTTCTCACCGACAGCATACTTATGTGCATGAGTAACCTA CCGGAATGGATATTGGCAGTAGAGAATACTTGCTTGGGAATTTACACATTCGAAATACTGGTAAAAGTCA TTGCAAGAGGTATCTGGGCAGGGTCATTTTCCTTCCTTGGGGATCTTTGGAACTGGCTTGATTTCAGTGT AACTTTGTTCGAGCTTATCACAAGGTCTTCACCTCTAAGCTCCCTTCCAATGTTTAAAACAATAAGAACC TTGCGAATTTTGAAGATCATCCCTTTGAACCATGGCCTGCAGTCTATTGTGGTGACGCTGGTCCAGTGTT TGAAGAAACTACTCGGAGCCATTGCCCTAGCTCTGTTTTTTCTGACTGTGTCTTCGCTATTCGGGATGGG TCTCTTCATGGGCAACCTGAAGCACAAATGTGTGCGATGGCCCCAAGAAGATGGAAATGATGTGATGTAC AGTGGAACTGGAAGCCAGTACCATATTCTAGAAAGAGAAAACTTCTACTATATGGAAGGCGCGAGATACG CTCTCCTCTGCGGCAACAAGACAGATGCCGGCCTGTGTCCAGAAGGTTATATGTGTGTAAAAGAGGGCTC AAATCCTGACAATGGCTTCACAAGTTTTGACAATTTTGGCTGGGCTCTACTGGCTATGTTTCGATTGATG ACTCAGGATTACCCTGAATTACTTTATCACCAGATCCTTTATGCTTCAGGAAAGATCTACATGATATTTT TTGTTCTGATCAGTTTCTGGTTTGCCTTCTATATGGCAAGTTTGTTCTTGGGCATCCTCACTATGGCCTA TGAACAGGAGAAGCAGAGAGCTTCTGAGGAGTCTAGAGACATGGATTCTAAATGTCATCAGACTGTGAAA GAATTCGAGGAAGAACATGAAGGAGCTGAGCTTCAGTGTATCTGGTTTTATGAGGAGGTTCTTGATCTAC TTGGACTTGAGCTTTGTATCAAGGAGATGGAAACTACACAAATAGAAATGAAGAAAAGATCACCAACTTC TATAAACACCACATTGGATATACTGGAAGACACTGCCCTTGGACACAAAGAAGAACCAGAAACATCCAGG AAGGAATGCCCACTCTGTTGGTATAAGTTTACTAAAACCTGCTTTATCTGGAAATGCTCTCCCTGTTGGA TAAAACTGAATGAGTTTGCTGACAGAATTATAACACACCCATTGTTTGATCTTTTCCTTGTTATATGCAT CATTTTAAACATATGCTTCCTGGCCCTAGAACACTTTCCAATGAGTGAGGAGCTCATGTCACTCCTTGCC ATTGGAAACTTGGTTTTTATTGGAATTTACACAATAGAAATGATTTTGAAGATAATCGCTATGCATCCAT ATGGGTATTTCCAAATAAGCTGGCATATTTTTGACAGTATTCTTGTGGTTTTGGGATTAACAGAAATGTT ACTAGCAGATATTGAAGAAATAACTGTTTTTATATTAGTCCCATTGATATTTATAAAATTGGGAAAATAT GCACCACCATTTAAGAATTTGATGCGTATTCTTGGTAGAGCATTGGTGGCCCTGAAAGACTTGGTCCTGT TGGTCTCCATTTTCATTTACTTCTCTGCCGTGTTTGGCATGAAGCTGTTTGGTCGAAGTTACAAAGATTG TGTCTGCCACGTAGATCAAGACTGTCAACGCCAACGTTGGCACATGAGTGACTTCCTCCACGCCTACGTG ACTGTGTTCCGAATCCTCTGCGGAGAGTGGATAGAGACATTATGGGAGTGTATGGAGGTTGCAGGCGAGG CCTGGTGTATTCCATTTTACATGATGGTCATTTTAATTGGAAATTTATTGATACTTTACCTCTTTGTGGG ATTGGTGAGCTCATTTGCTTCATACGATGCTACCACAGAAGTGAGCAAAGAAGCCAAAAACCTTCAGCTT GCAGTGGCATGGATAAAAATGGTAATAAACTGTGTGCTTCTTAAAATACTGTGCAAAGAAAAAACTGTTT CTACAGAAGCAACAGACCAAACATGTGATCCAAGTGTTAAAGAGAATATTTCTGGTCATACTCTTTCTGA ACTGAGCAACACCCAAACATTCCTCAGATACAAGGACCAGAGCAGTGGTACTGAGAAAACTCCAGTGACT GAGTCTGAGAGCCAATCCCTGATTGCTAGTCCCAGTGTATCAGAAACTGTGCCAATTGCTTCAGGAGAAT CCGATATAGAAAACCTGGATAACAAGGAGACTCGGAGCAAGTCTGCAAATGGAAGCAGTAAAGAGAAAAT GAAGCAATCTAGTTCATCTGAGTGCAGCACGGTTGATATTGCTATTTCTGAAGAAGAAGAAATGGTCTAT GAACATGAAAAGTCGAAGCTTCATAAAAATGGTTATGAACGCAAATCTTCAGCTGGCCAAGTCAGTAGAG AATCTAGGAATGGAAAGATTTGGAGGAACATAAGGAAAACTTGCTGCAAGATAGTGGAGAACAGCTGGTT TGAGTGTTTCATTGGCCTGGTCACTCTGCTCTGCACAGGCACTCTGGCTCTGGAAGACATATATATTCAT CAGCGGAAAACCATTAAAATCTTCCTGGAGTATGGAGACATGATATTTGCTTACATCTTCATTCTGGAGA TGCTTCTCAAGTGGGTGGCCTACGGCTTTAAAGCCTACTTCTCTAACAACTGGTACAAGCTGGACTTCAT GGTCGTTATCGTGTTGTGTCTTAGCTTAAAAGGCAAAACTCGAGAGGACCTGAACCCTCTGCCTTCCATA AAGTTCCTTCGAGCACTTAGAGTTCTGTCTCAGTTTGAAAGAATGAAGGTGGTTCTGAGAGCTTTGATAA AAACAACCTTACCCGCTGTGAGCGTGTTTCTGGTCTGCCTAATGATCTGGCTGCTTTTCAGTGTTATGGG AGTGTTCTTATTCGCTGGCAAGTTCTATGAATGCATTGACCCAACAAGGGGAGAAAGATTCTCTGTATTT GAAGTTATGAATAAGAGTCAGTGTGAAAACCTGGTATTTAATGAATCAATGCCATGGGAGAATGCAAAAC TGAACTTTGATAATGTTGGAAATGGTTTTCTTTCTTTGTTCCAAGTGGCAACATTTAATGGGTGGATCAG TATTATGAATTCAGCAATTGACTCTGTTGGTGTGTACATGCAGCCTAGTTTTGAGCACAGCCTCCACATG TACACTTACTTTATCATCTTTGTTGTGTTTGGATTATTTCTTCCCTTGTGTATGCTGATTGGTGTTATTA TCAGGAATTTCAACAAGCAGAAAATTAAGCAGGGAGGATCAAATATCTTTATAACGGTAAAGCAGAAGAA GCAGTACCGGGCACTGAAGAAGCTCTTGTATGCAGACTCTCAGAAACCAGCAGCCCGCCCTAGAAACAAG TTCCAAGGCTTCATTTGTGACGTAGTAACACATCGTGTCTTTAATGTCGTCGTCGTACTTCTTATCTGTT TCCAAGCAACAACCATTATGATACAAAATGATGAGCAGAGTCCACAAATTGAAACCGCTGTCTTCTGGAT GAACTCCCTTTTTACCATGCTGTTCACTCTGGAATGCATACTGAAGCTCACCGCCTTCCGCTGCCACTAT TTCACCAGCGCATGGAATGTTCATGACTTTATGGTGGTCGTTTTCTCCATTACAGGACTGCTGCTGCCTT TGTCAATAGGACAATACTTTGTGCCTCCTTCACTGGTGCAGCTGCTTCTTCTCTCTCGCATCATCCATGT CCTGCGTCCTGGGAAAGGACCAAAGGTGTTCCATGATCTGATGCTTCCCTTGATGCTGTCACTCCCAGCA CTGCTCAACATTGGTCTTCTCATCTTCCTGGTCATGTTCATCTACGCCATCTTTGGAATGTACAACTTCG CCTACGTGAAGAAAGAAGCCGGAATTAATGATGTGTCCAACTTTGAGACCTTTGGAAGCAGTATGCTTTG TCTCTTTCAAGTTACAACATTTTCTGGTTGGGACGGGATGCTGGATGCAATTTTCAACAGTCAGTGGTCT GACTGCGATCCTGATAAAATTAACCCAGGGACTCAGGTCAGGGGAGACTGTGGGAGCCCTTCTGTTGGGA TTTTTTATTTTGTCAGTTACATCCTCATATCATGGTTGATCATTGTTAATATGTACGTTGTTTTGATCAT GGAGTTCTTAAGTATTCCTTCTAAGAGAAAAAACAGGACCTTGAGCGAAGATGACTTTAGGAGATTCTTC AAGGTATGGAACAGGTTTGACCCTGACAGGACCCAGTACATAGACTCTACTAAGCTTTCTGATTTTGCAG CTGCTCTCGATCCTCCTCTTTTCATGGCAAAACCAAACAAGGGCCAGCTTGTAGCCATGGATCTCCCCAT GGCTGCAGGAGACAGAATCCATTGCCTTGATATTTTGCTTGCCTTTACGAAAAGAGTGATGGGAAAGGAT GAGCGGGTGGAGAAGATCCTTTCAGAGATAGAATCTGGGTTTATGTTAGCGAACCCTTTCAAAATTACGT ATGAGCCGATTACAACGACTTTGAAACGCAAACAAGAGGCAGTCTCAGCCACTATCATCCAGCGGGCATA CAAAAGTTACCGCTTAAGGCAAAGTGACAAAAAGATACAAGATATCCCGGAAATAGATGATGGCAGGGAG GATCCTAACTCTAAAGGTGTCCACTCTGGTCAAATCGAGGAAAAGGCATCTATTCAGACCCAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201560 representing NM_031686 Red=Cloning site Green=Tags(s) MLTSPEPKGLVPFTAESLELIKNHIAKKCNEEHEEEDLKPSRDIEAGKKLPFAYGTLPQGTVSEPLEDVD PYYYVKRNTFMVLNRNRVIFRFNAVSILCTLSPLSSLRRAVIKVLVHPLLRLLILISVLTDSILMCMSNL PEWILAVENTCLGIYTFEILVKVIARGIWAGSFSFLGDLWNWLDFSVTLFELITRSSPLSSLPMFKTIRT LRILKIIPLNHGLQSIVVTLVQCLKKLLGAIALALFFLTVSSLFGMGLFMGNLKHKCVRWPQEDGNDVMY SGTGSQYHILERENFYYMEGARYALLCGNKTDAGLCPEGYMCVKEGSNPDNGFTSFDNFGWALLAMFRLM TQDYPELLYHQILYASGKIYMIFFVLISFWFAFYMASLFLGILTMAYEQEKQRASEESRDMDSKCHQTVK EFEEEHEGAELQCIWFYEEVLDLLGLELCIKEMETTQIEMKKRSPTSINTTLDILEDTALGHKEEPETSR KECPLCWYKFTKTCFIWKCSPCWIKLNEFADRIITHPLFDLFLVICIILNICFLALEHFPMSEELMSLLA IGNLVFIGIYTIEMILKIIAMHPYGYFQISWHIFDSILVVLGLTEMLLADIEEITVFILVPLIFIKLGKY APPFKNLMRILGRALVALKDLVLLVSIFIYFSAVFGMKLFGRSYKDCVCHVDQDCQRQRWHMSDFLHAYV TVFRILCGEWIETLWECMEVAGEAWCIPFYMMVILIGNLLILYLFVGLVSSFASYDATTEVSKEAKNLQL AVAWIKMVINCVLLKILCKEKTVSTEATDQTCDPSVKENISGHTLSELSNTQTFLRYKDQSSGTEKTPVT ESESQSLIASPSVSETVPIASGESDIENLDNKETRSKSANGSSKEKMKQSSSSECSTVDIAISEEEEMVY EHEKSKLHKNGYERKSSAGQVSRESRNGKIWRNIRKTCCKIVENSWFECFIGLVTLLCTGTLALEDIYIH QRKTIKIFLEYGDMIFAYIFILEMLLKWVAYGFKAYFSNNWYKLDFMVVIVLCLSLKGKTREDLNPLPSI KFLRALRVLSQFERMKVVLRALIKTTLPAVSVFLVCLMIWLLFSVMGVFLFAGKFYECIDPTRGERFSVF EVMNKSQCENLVFNESMPWENAKLNFDNVGNGFLSLFQVATFNGWISIMNSAIDSVGVYMQPSFEHSLHM YTYFIIFVVFGLFLPLCMLIGVIIRNFNKQKIKQGGSNIFITVKQKKQYRALKKLLYADSQKPAARPRNK FQGFICDVVTHRVFNVVVVLLICFQATTIMIQNDEQSPQIETAVFWMNSLFTMLFTLECILKLTAFRCHY FTSAWNVHDFMVVVFSITGLLLPLSIGQYFVPPSLVQLLLLSRIIHVLRPGKGPKVFHDLMLPLMLSLPA LLNIGLLIFLVMFIYAIFGMYNFAYVKKEAGINDVSNFETFGSSMLCLFQVTTFSGWDGMLDAIFNSQWS DCDPDKINPGTQVRGDCGSPSVGIFYFVSYILISWLIIVNMYVVLIMEFLSIPSKRKNRTLSEDDFRRFF KVWNRFDPDRTQYIDSTKLSDFAAALDPPLFMAKPNKGQLVAMDLPMAAGDRIHCLDILLAFTKRVMGKD ERVEKILSEIESGFMLANPFKITYEPITTTLKRKQEAVSATIIQRAYKSYRLRQSDKKIQDIPEIDDGRE DPNSKGVHSGQIEEKASIQTQI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_031686
ORF Size	5106 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_031686.1, NP_113874.1
RefSeq Size	6556 bp
RefSeq ORF	5109 bp
Locus ID	64155
MW	194.7 kDa
Gene Summary	glial Na-channel alpha subunit [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.