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Phkb (NM_001014152) Rat Tagged ORF Clone
CAT＃: RR202189
- TrueORF®
Phkb (Myc-DDK-tagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug)
"NM_001014152" in other vectors (3)
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CNY 17,580.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202189 representing NM_001014152
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGGGAAGAGGGACTCATGTCTGAAGTAAGCTGGAAGCTCTTGGAACGACGAGCCCGGGCCAAGC GCTCAGGCTCAGTTTATGAACCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGA TAAGTTGGATCATTATTACAGAATTGTCAAGTCAACGATGCTGACATATCAAAGCCCAACTACAGGTCTC TTTCCTACTAAAACTTGTGGTGGAGATCAGAAGTCCAAAGTGCATGAGAGCCTGTACTGTGCTGCGGGTG CTTGGGCCTTGGCTCTGGCGTACAGGCGCATTGATGATGACAAGGGAAGGACCCATGAGCTGGAGCACTC GGCCATAAAGTGTATGAGAGGAGTTCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAGCAGTTCAAG CAGGATCCCCGGCCCACGACGTGTCTACACTCTGTTTTCAATGTGCACACTGGGGATGAAGTGCTCTCCT ATGAAGAATATGGCCATCTTCAGATAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTGGAAATGATTTC CTCAGGTCTCCAGATAATCTATAACACGGATGAGGTCTCTTTTATTCAAAACCTTGTGTTTTGTGTAGAG AGAGTTTACCGTGTTCCCGACTTCGGTGTCTGGGAAAGAGGAAGCAAATATAACAACGGCAGCACAGAAC TGCACTCGAGCTCTGTTGGTTTGGCAAAAGCAGCCTTAGAAGCGATTAATGGATTTAACCTCTTTGGAAA CCAGGGCTGTTCTTGGTCAGTTATATTCGTGGATCTCGATGCTCACAACCGCAACAGGCAGACGTTGTGT TCACTGTTACCCAGAGAATCACGCTCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCACCTACCCCG CCTTTGCCCTGGACGATGAAGCTCTGTTCAGCCAGACACTCGATAAAGTGATAAGAAAATTAAAAGGGAA ATATGGATTTAAGCGTTTCTTGCGAGATGGGTACAGAACGCCCCTGGAAGACCCCAACAGACGCTACTAC AAACCAGCGGAGATTAAGCTGTTTGATGGCATCGAATGTGAATTTCCCATATTCTTCCTTTACCTGATGA TCGATGGTGTTTTTAGAGGCAATCTTGAACAAGTCAAGGAATATCAGGACCTGTTGACACCTTTGCTTCA CCAGACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAA AAAAGAAACCCTGGTAGTCAGAAGCGGTTTCCTAGTAACTGTGGCCGTGATGGGAAATTGTTTCTTTGGG GACAAGCTCTTTATATCATTGCAAAACTCCTAGCTGATGAGTTAATCAGTCCTAAAGACATTGACCCCGT CCATCGCTTTGTCCCACTTCAGAATCAACGTAACGTGAGCATGAGATTCTCTAATCAGGGCCCACTGGAA AATGACTTGGTGGTTCATGTGGCACTTGTAGCAGAAAGCCAACGCCTTCAGGTCTTCCTCAATACTTACG GGATTCAGACTCAAACGCCCCAGCAGGTGGAGCCTATTCAGATCTGGCCTCAGCAGGAGCTCGTGAAAGC TTATTTCCACCTGGGTATCAATGAAAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTT GGTACTTCAAAGATTTACCGCATCCTAGGAAGGACCGTGGTTTGTTACCCTATCATCTTCGACCTCAGTG ATTTCTACATGTCTCAGGACGTTCTCCTGCTCATAGATGACATAAAGAATGCACTACAGTTCATTAAGCA GTATTGGAAAATGCATGGACGCCCACTTTTTCTTGTTCTCATCCGGGAAGACAACATAAGAGGTAGCAGA TTCAACCCCATATTAGACATGCTGGCAGCCTTTAAAAAGGGGATAATTGGAGGAGTCAAAGTTCATGTTG ACCGTTTACAGACATTGATATCTGGAGCTGTAGTAGAACAGCTTGACTTTCTGCGGATTAGCGACACAGA AAAGCTTCCAGAGTTTAAGAGCTTTGAAGAACTAGAATTTCCCAAGCATTCAAAAGTCAAACGACAGAGC AGTACTGCAGATGCTCCTGAACTGCGACAGGAGCCAGACATCACCATCACAGAGTGGAAAAACAAATCCA CCCACGAAATCCTTCAGAAGCTTAATGACTGCGGTTGTCTGGCTGGCCAAACCATCCTGCTGGGTATACT GCTCAAAAGAGAGGGCCCTAACTTCATCACGATGGAAGGAACTGTGTCTGATCACATTGAGAGGGTGTAC AGAAGAGCTGGCAGCAAAAAGCTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGG ACAGCCTGGCCCCATCCATTACTAATGTTTTAGTGCAGGGCAAACAGGTAACCCTAGGTGCCTTTGGACA CGAAGAGGAGGTCATCTCTAATCCACTATCTCCAAGAGTGATTAAGAACATCATCTATTATAAGTGTAAC ACCCATGATGAGAGGGAGGCTGTCATTCAGCAAGAGCTGGTCATCCATATTGGCTGGATCATCTCCAACA ACCCCGAGCTGTTCAGTGGCATGCTGAAAATCAGAATTGGGTGGATCATCCATGCCATGGAGTACGAACT GCAGATCCGTGGCGGGGATAAGCCAGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAGGTTAAGCAGCTG CTGCTCGACATCCTGCAGCCTCAGCAGAGTGGGAGATGTTGGCTGAATAGACGTCAGATTGATGGGTCAC TGAATAGAACACCCCCAGGATTCTACGACCGCGTGTGGCAGATCCTGGAGCGCACACCTAATGGTATCGT TGTAGCTGGCAAGCATTTGCCACAGCAACCAACCTTGTCAGATATGACCATGTATGAGATGAATTTTTCT CTCCTTGTTGAAGATATGTTGGGAAACATTGACCAGCCAAAGTATAGACAGATCATTGTAGAGTTACTAA TGGTTGTATCCATTGTGCTGGAAAGAAATCCTGAGCTAGAATTTCAAGACAAAGTGGATCTGGACAAACT GGTCAAAGAAGCGTTTCATGAGTTTCAAAAAGATGAGAGCCGGCTAAAAGAAATCGAAAAGCAAGTAAGT GGAGAGGCCCGTGTCCCACAGAACGCTTTCATTGTTTCCACTGTTGAAATCCTCTCTAATCTTCCCACTT TCCAGACAGATGGACAGAGCACAAAAGAGGATGCTCTACAAGCAGTCAGGCTTACTGAGGAATTAATATT GCTTTTTAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202189 representing NM_001014152
Red=Cloning site Green=Tags(s) MAGEEGLMSEVSWKLLERRARAKRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLTYQSPTTGL FPTKTCGGDQKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGVLYCYMRQADKVQQFK QDPRPTTCLHSVFNVHTGDEVLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVE RVYRVPDFGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLC SLLPRESRSHNTDAALLPCITYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYY KPAEIKLFDGIECEFPIFFLYLMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVEYE KRNPGSQKRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVHRFVPLQNQRNVSMRFSNQGPLE NDLVVHVALVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCL GTSKIYRILGRTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSR FNPILDMLAAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQS STADAPELRQEPDITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVY RRAGSKKLWSVVRRAASLLNKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCN THDEREAVIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPAVDLYQLSPSEVKQL LLDILQPQQSGRCWLNRRQIDGSLNRTPPGFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFS LLVEDMLGNIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDKLVKEAFHEFQKDESRLKEIEKQVS GEARVPQNAFIVSTVEILSNLPTFQTDGQSTKEDALQAVRLTEELILLFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001014152 |
| ORF Size | 3300 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001014152.1, NP_001014174.1 |
| RefSeq Size | 3630 bp |
| RefSeq ORF | 3303 bp |
| Locus ID | 361377 |
| MW | 125.9 kDa |
| Gene Summary | expression in regenerating muscle may be correlated with that of other phosphorylase kinase subunits [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202189 | Phkb (untagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug) |
CNY 17,580.00 |
|
| RR202189L3 | Lenti ORF clone of Phkb (Myc-DDK-tagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug) |
CNY 19,290.00 |
|
| RR202189L4 | Lenti ORF clone of Phkb (mGFP-tagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug) |
CNY 21,000.00 |
