Phkb (NM_001014152) Rat Untagged Clone

CAT#: RN202189

Phkb (untagged ORF) - Rat phosphorylase kinase, beta (Phkb), (10 ug)



  "NM_001014152" in other vectors (3)

CNY 17,580.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN202189 representing NM_001014152
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGGGAAGAGGGACTCATGTCTGAAGTAAGCTGGAAGCTCTTGGAACGACGAGCCCGGGCCAAGC
GCTCAGGCTCAGTTTATGAACCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGA
TAAGTTGGATCATTATTACAGAATTGTCAAGTCAACGATGCTGACATATCAAAGCCCAACTACAGGTCTC
TTTCCTACTAAAACTTGTGGTGGAGATCAGAAGTCCAAAGTGCATGAGAGCCTGTACTGTGCTGCGGGTG
CTTGGGCCTTGGCTCTGGCGTACAGGCGCATTGATGATGACAAGGGAAGGACCCATGAGCTGGAGCACTC
GGCCATAAAGTGTATGAGAGGAGTTCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAGCAGTTCAAG
CAGGATCCCCGGCCCACGACGTGTCTACACTCTGTTTTCAATGTGCACACTGGGGATGAAGTGCTCTCCT
ATGAAGAATATGGCCATCTTCAGATAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTGGAAATGATTTC
CTCAGGTCTCCAGATAATCTATAACACGGATGAGGTCTCTTTTATTCAAAACCTTGTGTTTTGTGTAGAG
AGAGTTTACCGTGTTCCCGACTTCGGTGTCTGGGAAAGAGGAAGCAAATATAACAACGGCAGCACAGAAC
TGCACTCGAGCTCTGTTGGTTTGGCAAAAGCAGCCTTAGAAGCGATTAATGGATTTAACCTCTTTGGAAA
CCAGGGCTGTTCTTGGTCAGTTATATTCGTGGATCTCGATGCTCACAACCGCAACAGGCAGACGTTGTGT
TCACTGTTACCCAGAGAATCACGCTCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCACCTACCCCG
CCTTTGCCCTGGACGATGAAGCTCTGTTCAGCCAGACACTCGATAAAGTGATAAGAAAATTAAAAGGGAA
ATATGGATTTAAGCGTTTCTTGCGAGATGGGTACAGAACGCCCCTGGAAGACCCCAACAGACGCTACTAC
AAACCAGCGGAGATTAAGCTGTTTGATGGCATCGAATGTGAATTTCCCATATTCTTCCTTTACCTGATGA
TCGATGGTGTTTTTAGAGGCAATCTTGAACAAGTCAAGGAATATCAGGACCTGTTGACACCTTTGCTTCA
CCAGACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAA
AAAAGAAACCCTGGTAGTCAGAAGCGGTTTCCTAGTAACTGTGGCCGTGATGGGAAATTGTTTCTTTGGG
GACAAGCTCTTTATATCATTGCAAAACTCCTAGCTGATGAGTTAATCAGTCCTAAAGACATTGACCCCGT
CCATCGCTTTGTCCCACTTCAGAATCAACGTAACGTGAGCATGAGATTCTCTAATCAGGGCCCACTGGAA
AATGACTTGGTGGTTCATGTGGCACTTGTAGCAGAAAGCCAACGCCTTCAGGTCTTCCTCAATACTTACG
GGATTCAGACTCAAACGCCCCAGCAGGTGGAGCCTATTCAGATCTGGCCTCAGCAGGAGCTCGTGAAAGC
TTATTTCCACCTGGGTATCAATGAAAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTT
GGTACTTCAAAGATTTACCGCATCCTAGGAAGGACCGTGGTTTGTTACCCTATCATCTTCGACCTCAGTG
ATTTCTACATGTCTCAGGACGTTCTCCTGCTCATAGATGACATAAAGAATGCACTACAGTTCATTAAGCA
GTATTGGAAAATGCATGGACGCCCACTTTTTCTTGTTCTCATCCGGGAAGACAACATAAGAGGTAGCAGA
TTCAACCCCATATTAGACATGCTGGCAGCCTTTAAAAAGGGGATAATTGGAGGAGTCAAAGTTCATGTTG
ACCGTTTACAGACATTGATATCTGGAGCTGTAGTAGAACAGCTTGACTTTCTGCGGATTAGCGACACAGA
AAAGCTTCCAGAGTTTAAGAGCTTTGAAGAACTAGAATTTCCCAAGCATTCAAAAGTCAAACGACAGAGC
AGTACTGCAGATGCTCCTGAACTGCGACAGGAGCCAGACATCACCATCACAGAGTGGAAAAACAAATCCA
CCCACGAAATCCTTCAGAAGCTTAATGACTGCGGTTGTCTGGCTGGCCAAACCATCCTGCTGGGTATACT
GCTCAAAAGAGAGGGCCCTAACTTCATCACGATGGAAGGAACTGTGTCTGATCACATTGAGAGGGTGTAC
AGAAGAGCTGGCAGCAAAAAGCTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGG
ACAGCCTGGCCCCATCCATTACTAATGTTTTAGTGCAGGGCAAACAGGTAACCCTAGGTGCCTTTGGACA
CGAAGAGGAGGTCATCTCTAATCCACTATCTCCAAGAGTGATTAAGAACATCATCTATTATAAGTGTAAC
ACCCATGATGAGAGGGAGGCTGTCATTCAGCAAGAGCTGGTCATCCATATTGGCTGGATCATCTCCAACA
ACCCCGAGCTGTTCAGTGGCATGCTGAAAATCAGAATTGGGTGGATCATCCATGCCATGGAGTACGAACT
GCAGATCCGTGGCGGGGATAAGCCAGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAGGTTAAGCAGCTG
CTGCTCGACATCCTGCAGCCTCAGCAGAGTGGGAGATGTTGGCTGAATAGACGTCAGATTGATGGGTCAC
TGAATAGAACACCCCCAGGATTCTACGACCGCGTGTGGCAGATCCTGGAGCGCACACCTAATGGTATCGT
TGTAGCTGGCAAGCATTTGCCACAGCAACCAACCTTGTCAGATATGACCATGTATGAGATGAATTTTTCT
CTCCTTGTTGAAGATATGTTGGGAAACATTGACCAGCCAAAGTATAGACAGATCATTGTAGAGTTACTAA
TGGTTGTATCCATTGTGCTGGAAAGAAATCCTGAGCTAGAATTTCAAGACAAAGTGGATCTGGACAAACT
GGTCAAAGAAGCGTTTCATGAGTTTCAAAAAGATGAGAGCCGGCTAAAAGAAATCGAAAAGCAAGTAAGT
GGAGAGGCCCGTGTCCCACAGAACGCTTTCATTGTTTCCACTGTTGAAATCCTCTCTAATCTTCCCACTT
TCCAGACAGATGGACAGAGCACAAAAGAGGATGCTCTACAAGCAGTCAGGCTTACTGAGGAATTAATATT
GCTTTTTAGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001014152
Insert Size 3303 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001014152.1, NP_001014174.1
RefSeq Size 3630 bp
RefSeq ORF 3303 bp
Locus ID 361377
Gene Summary expression in regenerating muscle may be correlated with that of other phosphorylase kinase subunits [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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