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Vps26a (NM_001007740) Rat Tagged ORF Clone
CAT＃: RR202230
- TrueORF®
Vps26a (Myc-DDK-tagged ORF) - Rat vacuolar protein sorting 26 homolog A (S. pombe) (Vps26a), (10 ug)
"NM_001007740" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Vps26 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202230 representing NM_001007740
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTTTCTTGGAGGCTTTTTTGGTCCCATCTGTGAGATCGATGTTGCCCTTAGTGATGGGGAGACCA GGAAAATGGCAGAAATGAAAACGGAAGATGGCAAAGTAGAAAAACACTATCTCTTCTATGATGGGGAATC TGTCTCAGGAAAGGTAAACCTAGCCTTTAAGCAGCCTGGAAAGAGGCTAGAGCATCAAGGAATTAGAATT GAATTTGTAGGTCAAATTGAGCTTTTCAATGACAAGAGTAATACTCATGAATTTGTAAACCTAGTGAAGG AACTAGCCTTGCCTGGAGAGCTGACTCAGAGCAGAAGCTATGACTTTGAATTCATGCAAGTTGAAAAGCC ATATGAGTCATACATCGGTGCCAATGTCCGCCTGAGGTATTTCCTTAAGGTGACCATTGTGAGAAGACTG ACAGACTTAGTGAAAGAGTACGATCTTATTGTTCACCAGCTCGCCACCTACCCAGAGGTCAACAACTCCA TTAAAATGGAGGTGGGAATTGAAGACTGTCTGCACATAGAGTTTGAGTACAATAAGTCCAAGTATCATTT AAAGGATGTAATTGTTGGAAAAATTTACTTCTTATTAGTAAGAATAAAAATACAACACATGGAGTTACAG CTGATCAAGAAAGAGATCACAGGAATTGGACCCAGCACCACAACAGAGACAGAAACAATCGCTAAGTATG AAATAATGGATGGGGCGCCAGTAAAAGGAGAATCTATTCCGATAAGATTGTTCTTAGCAGGGTATGACCC AACCCCCACAATGAGAGATGTGAACAAGAAGTTTTCAGTAAGGTACTTTTTGAACCTCGTGCTTGTTGAT GAGGAGGACAGAAGGTACTTCAAGCAGCAGGAGATTATCCTGTGGAGAAAAGCACCTGAGAAACTGAGAA AACAGAGGACGAACTTTCACCAGCGATTTGAATCTCCAGAATCGCAGGCGTCTGCAGAACAGCCTGAGAT G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202230 representing NM_001007740
Red=Cloning site Green=Tags(s) MSFLGGFFGPICEIDVALSDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRI EFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRL TDLVKEYDLIVHQLATYPEVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQ LIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVD EEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001007740 |
| ORF Size | 981 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001007740.1, NP_001007741.1 |
| RefSeq Size | 1292 bp |
| RefSeq ORF | 984 bp |
| Locus ID | 361846 |
| UniProt ID | Q6AY86 |
| MW | 38.1 kDa |
| Gene Summary | Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3.The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins. The CSC complex seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5. Required for retrograde transport of lysosomal enzyme receptor IGF2R. Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Required for the endosomal localization of WASHC2 (indicative for the WASH complex). Required for the endosomal localization of TBC1D5. Mediates retromer cargo recognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP. Involved in retromer-independent lysosomal sorting of F2R. Involved in recycling of ADRB2. Acts redundantly with VSP26B in SNX-27 mediated endocytic recycling of SLC2A1/GLUT1. Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202230 | Vps26a (untagged ORF) - Rat vacuolar protein sorting 26 homolog A (S. pombe) (Vps26a), (10 ug) |
CNY 3,990.00 |
|
| RR202230L3 | Lenti ORF clone of Vps26a (Myc-DDK-tagged ORF) - Rat vacuolar protein sorting 26 homolog A (S. pombe) (Vps26a), (10 ug) |
CNY 6,080.00 |
|
| RR202230L4 | Lenti ORF clone of Vps26a (mGFP-tagged ORF) - Rat vacuolar protein sorting 26 homolog A (S. pombe) (Vps26a), (10 ug) |
CNY 6,650.00 |
