Abcc10 (NM_001108201) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202266 representing NM_001108201 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGCTCCTGGCCCAGCTTTGTGGCACGGATGCGGCGCGGCCACTCCCGCTCTGGGAAGGGGACA CCACGGGCCACTGCTTCACCCAGCTGGTGCTCAGCGTCCTTCCACACGCTCTCCTCGCCGTCCTCAGCGC CTGCCACTTGGGCACCCCAAGGACTAACAATATCATCCAACCTTTCAATCCTGGCTGGCGCCTCCGACTC ACAGCTTCCTTCCTCCTCTCCGTCTTCCCGCTGCTAGACCTCCTTCCGGTTGTTTTACCTCCGGTGTCAC ACCCAGGGCCCCTATGGCTAGAAGTGCTGGCAGGGTGTGTGACTGCTGTGGCCTGGTTCACCCACAGCCT GGCCCTGTGGGCATTGGTTCATTCCCCTCATGGCCATTCTCGTGGACCCTTGGCCTTGGCTCTGGCAGCC TTCTTGCCAACCCCAGCCCTGGTACTGACCCTGCTGTGGCATTGCCAGCGAGGTACATTTCTGCCCCCGC TTCTCCCAGGACCTCTGGGCCGTGTGTGTCTTCTCATCCTGCAGTTGGCAGCCGTCTTGGCCTATGGGCT GGGCTGGGCAGCCCCTGGGGGACCTCGAGAGCCCTGGACTCACGATCCCTTCCTGTCCCCTGAGAGTCAA GAAAGAGAGGTAGCTGAAGACGGAGAGAGTTGGCTGTCACGCTTTTCCTATGCCTGGTTGGCACCTCTGC TTGCTCGTGGAGTCCGAGGAGAGCTCCGGCAGCCCCAGGACACTTGCCGCCTCCCCCGCAGACTGCACCC TGCCTACCTGGCCCGAGCCTTCCAAGCGCACTGGAAGGAGGGGGCCCAACTGTGGAGGGCCCTGTATGGG GCCTTTGGATGTTGCTACATAGCCCTTGGACTGTTGAAGATGGTGGGGACCATGCTGGGCTTCTCTGGGC CTTTACTGCTCTCCCTACTGGTGGGCTTCCTGGAGGAAGGGCAAGAGCCTCTAAGCCACGGCCTGCTCTA CGTCCTGGGGCTGGCCAGTGGGTCTGTGATAAGCGCCGTACTACAGAACCAGTATGGGTATGAGGTGCGT AAAGTGACACTTCAGGCACGGGTGGCTGTGCTGAGCATCCTTTACCGCAAAACCTTGAAGCTTGGGCCTA GCCGCCCTCCCACTGGGGAGGTCCTGAACCTACTAGGCACCGACTCCGAGAGGCTGCTTAACTTTGCTGG CAGCTTCCATGAGGCCTGGGGCCTGCCTCTGCAACTGGCCATCACTCTCTACCTGCTGTACGAGCAGGTG GGCGTGGCCTTCCTGGCTGGGTTAGTCTTGGCACTGCTCCTGGTGCCTGTCAACAAAGTGATCGCCACCC GAATCATGGCCAACAACCAGGAGATGCTCCGGCACAAGGATGCACGGGTTAAGCTCATGACAGAGCTGCT GAGCGGCATTCGAGTCCTCAAGTTCTTCAGGTGGGAGCAGGCTCTGGGGGACCGGGTGAAGGCTTGCCGG ACCCAAGAGCTGGGACGGCTCCGCGTCATCAAATACCTGGATGCAGCCTGCGTGTACCTCTGGGCTGCTC TGCCTGTAGTCATCTGCATCGTCATCTTCATCACCTACGTCCTCCTGGGGCACCAGCTCACTGCCACCAA GGTGTTTACAGCACTGGCACTTGTGCACATGCTCATTCTTCCTCTCAACAACTTCCCCTGGGTGATCAAT GGTCTCTTGGAATCCAAAGTATCCCTGGACCGGATCCAGCGTTTCCTCGACCTTCCAAGCTATAGCCCTG AGGCCTACTACAGTCCTGATCCCCCCACAGAGCCATCCACAGTACTGGAGCTGCATGAAGCCCTGTTCTC CTGGGACCCAACTGGAACAAGCCAGAAGACCTTCATCAGTCACCTCCAAGTGAAAAAGGGCATGCTGGTG GGCATCGTGGGAAAGGTCGGCTGTGGGAAGAGTTCACTGCTGGCTGCCATCACTGGGGAGCTCCACAGGC TGTGTGGGTGGGTGGCAGTGTCAGACCTGTCCAAAGGCTTTGGACTGGCCACTCAGGAGCCCTGGATCCA GTGTGCCACCATCAGAGACAACGTCCTGTTTGGGAAGACATTTGATGCCCAGCTGTACAGGGAGGTGCTG GAGGCCTGCGCCCTCAATGATGATCTCAGCATCCTGCCTGCTGGAGACCAGACAGAGGTTGGAGAGAAGG GTGTGACCCTCAGCGGGGGACAGAGGGCCCGGATTGCCCTTGCCCGAGCTGTCTACCAGGAGAAAACACT CTACCTCCTTGATGACCCTCTGGCTGCTGTGGATGCAGATGTGGCCAACCACCTGTTGCACAGGTGCATC CTGGGAGTGCTGGGCCACACCACCCGGCTGCTGTGCACCCATCGCACCGAGTACCTGGAGAGGGCTGACC TGGTGCTGTTGATGGAGGCTGGGCGCCTGGTCCGAGCAGGGCCTCCCTCAGAGATTCTGCCATTGGTGCA AGCTGCCCCCACAGCCTTGGCTGACAAGGAACAAGTGACTGACTCAGGTCAGTCCCCGTCTGTACACAAC CTAGAGAAAACCACAGAGGAGCTGGACGTGGCGCAGAACACATCCGGTCGCCTCGTGCAGGAGGAGAGCA AAAGCGAGGGCGCCGTGGCCCTGCACGTGTACCGAGCATACTGGAGGGCCATGGGCAGTGGCCTGGCCAC CGCCATCCTCATCTCCCTGCTCCTCATGCAAGCCACGCGCAACGGTGCTGATTGGTGGCTCGCTCACTGG CTCTCTCAGCTGAAGGCAGGCAGAAATAGCTCCGAGGAGCGTCCCGCTTCCTCCAGCCCACGCTCCATGG GATTCTTCTCCCCACGGCTGCTCCTCTTCTCCCCCGGAAACCTCTACATCCCACTGCACAAAGCTACTTC CAATGGCTCCTCAGACGTCCACTTCTACCTCACCGTGTACGCAACCATTGCTGGTGTCAATTCCCTCTGC ACCCTGCTCCGGGCTGTGCTCTTTGCAGCAGGTGCCCTTCAAGCAGCTGCTAGCCTACACCACCGTCTAC TGCACCGACTGCTCATGGCACCAGTGACTTTCTATGACTCCACACCTTCCGGCCGGGTCTTGAACCGCTT CTCCTCAGACGTCGCCTGTGTAGACGACAGCCTGCCCTTCCTCCTCAACATCCTGCTGGCCAATTCTGTA GGGCTGCTGGGCCTCCTCGCTGTCCTGGGTTCTGGTCTGCCCTGGCTGCTGCTCCTGCTGCCGCCTCTGA GCTTCATCTACTACGGCGTGCAGCGCCGCTACAGGGCTTCCTTCCGGGAGCTGCGGCGTCTGGGCAGCCT CACCTTGTCTCCGCTCTACTCCCACCTGGCTGACACCCTGGCAGGCCTCCCTGTGCTCCGGGCTGCAGGG GCCACTTACAGGTTTGAGGAGGAGAACCAGCGACTCTTGGAGCTGAACCAGAGGTGCCAGTTTGCTTCCT ATGCCACGATGCAGTGGTTGGACATTCGGCTGCAGCTCATGGGGGCAGCGGTGGTCAGCGCCATTGCGGG CATTGCCCTGGTGCAGCACCAGCAGGGCCTTGCCAACCCAGGGCTGGTGGGCCTCGTGCTTTCTTATGCA CTGTCTCTGACGGGCCTGCTCTCCGGCCTGGTGAGCAGCTTCACACAGACGGAAGCCATGATGGTGAGCG TTGAACGACTGGAGGAATATTCCTGTGACATTCCCCAGGAGCCCCATGGCCAGCCACCACAGTCACCCCA CCAGCGCGTCAGCTGGCTGACCCAGGGAAGTGTGGAGTTCCAGGATGTGGTGCTGGTGTATCGGCCAGGG CTGCCAAATGCCCTAGACGGGGTGACCTTTCGCGTGGAGCCTGGCGAGAAGCTGGGCATTGTGGGCCGCA CAGGCTCCGGCAAGTCTTCCCTGTTTCTGGTGCTCTTCCGGCTGCTGGAGCCCAGTGCAGGGCAAGTGCT CCTGGACGGTGTGGACACCAGCCAGCTGGAGCTGGCTGAGCTCAGATCCCAGCTGGCTGTCATCCCCCAG GAGCCCTTCTTGTTCAGTGGGACCGTTCGGGAGAACCTGGACCCCCAGGGCCTACATGAGGACAGGGCCC TGTGGCAAGCCCTAGAACAGTGCCACCTGAGTGAGGTGGCCATGGCCATAGGAGGCCTGGATGGGGAGCT GGGCGAAAGGGGCAGGGACTTGTCCCTAGGGCAGAGACAGCTGCTATGCCTGGCAAGGGCTCTTCTCACA GATGCTAAGATCTTGTGCATTGACGAGGCCACGGCAAGTGTGGACCAGAAGACAGACCAGCTGCTCCAGC AAACCATCTGCAGACGCTTTGCCAACAAGACAGTGCTGACCATTGCCCACAGGCTCAACACGATCCTAAA CTCTGACCGGGTGCTGGTGCTCCAAGCCGGGAGGGTGGTGGAACTGGACTCCCCCTCAGCCCTGCGCAAC CAGCCCCATTCCCTGTTTAGGCAGCTCCTGCAGAGCAGCCAGCAGGGGGCCCACTCCCTTCCTGCCGCGT GC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202266 representing NM_001108201 Red=Cloning site Green=Tags(s) MEGLLAQLCGTDAARPLPLWEGDTTGHCFTQLVLSVLPHALLAVLSACHLGTPRTNNIIQPFNPGWRLRL TASFLLSVFPLLDLLPVVLPPVSHPGPLWLEVLAGCVTAVAWFTHSLALWALVHSPHGHSRGPLALALAA FLPTPALVLTLLWHCQRGTFLPPLLPGPLGRVCLLILQLAAVLAYGLGWAAPGGPREPWTHDPFLSPESQ EREVAEDGESWLSRFSYAWLAPLLARGVRGELRQPQDTCRLPRRLHPAYLARAFQAHWKEGAQLWRALYG AFGCCYIALGLLKMVGTMLGFSGPLLLSLLVGFLEEGQEPLSHGLLYVLGLASGSVISAVLQNQYGYEVR KVTLQARVAVLSILYRKTLKLGPSRPPTGEVLNLLGTDSERLLNFAGSFHEAWGLPLQLAITLYLLYEQV GVAFLAGLVLALLLVPVNKVIATRIMANNQEMLRHKDARVKLMTELLSGIRVLKFFRWEQALGDRVKACR TQELGRLRVIKYLDAACVYLWAALPVVICIVIFITYVLLGHQLTATKVFTALALVHMLILPLNNFPWVIN GLLESKVSLDRIQRFLDLPSYSPEAYYSPDPPTEPSTVLELHEALFSWDPTGTSQKTFISHLQVKKGMLV GIVGKVGCGKSSLLAAITGELHRLCGWVAVSDLSKGFGLATQEPWIQCATIRDNVLFGKTFDAQLYREVL EACALNDDLSILPAGDQTEVGEKGVTLSGGQRARIALARAVYQEKTLYLLDDPLAAVDADVANHLLHRCI LGVLGHTTRLLCTHRTEYLERADLVLLMEAGRLVRAGPPSEILPLVQAAPTALADKEQVTDSGQSPSVHN LEKTTEELDVAQNTSGRLVQEESKSEGAVALHVYRAYWRAMGSGLATAILISLLLMQATRNGADWWLAHW LSQLKAGRNSSEERPASSSPRSMGFFSPRLLLFSPGNLYIPLHKATSNGSSDVHFYLTVYATIAGVNSLC TLLRAVLFAAGALQAAASLHHRLLHRLLMAPVTFYDSTPSGRVLNRFSSDVACVDDSLPFLLNILLANSV GLLGLLAVLGSGLPWLLLLLPPLSFIYYGVQRRYRASFRELRRLGSLTLSPLYSHLADTLAGLPVLRAAG ATYRFEEENQRLLELNQRCQFASYATMQWLDIRLQLMGAAVVSAIAGIALVQHQQGLANPGLVGLVLSYA LSLTGLLSGLVSSFTQTEAMMVSVERLEEYSCDIPQEPHGQPPQSPHQRVSWLTQGSVEFQDVVLVYRPG LPNALDGVTFRVEPGEKLGIVGRTGSGKSSLFLVLFRLLEPSAGQVLLDGVDTSQLELAELRSQLAVIPQ EPFLFSGTVRENLDPQGLHEDRALWQALEQCHLSEVAMAIGGLDGELGERGRDLSLGQRQLLCLARALLT DAKILCIDEATASVDQKTDQLLQQTICRRFANKTVLTIAHRLNTILNSDRVLVLQAGRVVELDSPSALRN QPHSLFRQLLQSSQQGAHSLPAAC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108201
ORF Size	4482 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108201.1, NP_001101671.1
RefSeq Size	4859 bp
RefSeq ORF	4485 bp
Locus ID	316231
MW	163.3 kDa

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