Abraxas1 (NM_001009633) Rat Tagged ORF Clone

CAT#: RR202541

  • TrueORF®

Fam175a (Myc-DDK-tagged ORF) - Rat coiled-coil domain containing 98 (Ccdc98), (10 ug)



  "NM_001009633" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Ccdc98; Fam175a; RGD1305287
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR202541 representing NM_001009633
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCGAGAGCACGTTGGGGGTGTTGTCTGGCTTCGTGCTTGGCGCGCTCACCTTCCAGCATCTCA
ACACTGACTCAGATACGGAGGGTCTTCTCCTCGGTGAAATGAAAGGTGAAGCCAAGAATAGCATCACTGA
CTCACAGATGGACAGCGTTAAAGTCGTCTACACAATTGACATTCAGAAATATATTCCATGCTACCGGCTT
TTTAGCTTTTATAATTCTTTAGGTGAAGTAAATGAACACGCACTGAAGAAAATTCTTTCAAATGTCAAAA
AGACTGTGGTGGGTTGGTATAAATTCCGTCGTCACTCGGATCAGATAATGACATTTAGAGAGCAGTTGCT
CCACAGAAACTTACAGACGCATCTTTCAAGCCCAGAGCTTGTTTTTCTGTTGTTAACACCAAGTATAACA
ACAGAGAGCTGCTGCACTCACTGCCTGGAACACGGCCTGTACAAACCTCAAAGTGGGCTTTTTCATAAGG
TACCTTTGGTGGTTACCAATCTGGGAATGTCTGATCAACTGGGTTATAAAACAGAGTCTGTTTCCTGTAC
GTCCACTGTCTTTAGCAGAGCTGTGAGGACATACAGTTCTCAATTTTTTAATGAAGATGGATCATTAAAG
GAAGTACGTAAGATAAATGAAATGTATGCTGCTATACAAGAAGAATTAAAGACTATATGCCAAAAAGTTG
AACAAAGTGAACGAGAAGTAGAGAAACTATTGATGGATGTAAACCGATTAAAAGAAGTTAGAAAAAAACA
GCAAGCACAGGCTAAAGGAGCAGGAGAGAAGAGCCAAAACCATCCCCAGGAGAACATTCTCCTCTGCCAA
GCTTTACGAACATTCTTTCCAGAGTCTCGAGTTCTTCACTCTTGCGTTATTTCTTTGAAAAATAGACATA
TTTCTCACAGTGGGTGTAACACTGACCACCATCTTGATGTGGTGGATAAGCTAACCTTAATGGTGGAGTA
CGTTTACAGCCCTGAAGCTAGTCCAGCACCTGCAGCCCCACTGAGCAAACGTAAAGCTCTAGACACACAA
GATCAGTGGCCTGCGAAGAGACCACGGCTGCTAGAGTCAGAAAGCAGACCTGGGCCAGCATTTCGAGGCA
GTCACCAGGACAAAGCCTCCTCAAGCAGCCTCGATATAGACACAGAGGTGGGGAGCCCGGAAGATGATAC
TGACTACCCTCGCTCTCCCACATTTTGACTACCCTCGCTCTCCCACATTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR202541 representing NM_001009633
Red=Cloning site Green=Tags(s)

MEGESTLGVLSGFVLGALTFQHLNTDSDTEGLLLGEMKGEAKNSITDSQMDSVKVVYTIDIQKYIPCYRL
FSFYNSLGEVNEHALKKILSNVKKTVVGWYKFRRHSDQIMTFREQLLHRNLQTHLSSPELVFLLLTPSIT
TESCCTHCLEHGLYKPQSGLFHKVPLVVTNLGMSDQLGYKTESVSCTSTVFSRAVRTYSSQFFNEDGSLK
EVRKINEMYAAIQEELKTICQKVEQSEREVEKLLMDVNRLKEVRKKQQAQAKGAGEKSQNHPQENILLCQ
ALRTFFPESRVLHSCVISLKNRHISHSGCNTDHHLDVVDKLTLMVEYVYSPEASPAPAAPLSKRKALDTQ
DQWPAKRPRLLESESRPGPAFRGSHQDKASSSSLDIDTEVGSPEDDTDYPRSPTF*LPSLSHI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001009633
ORF Size 1215 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 1513 bp
RefSeq ORF 1218 bp
Locus ID 289468
UniProt ID Q5I0F1
MW 45.7 kDa
Gene Summary Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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