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Brf1 (NM_001106761) Rat Tagged ORF Clone
CAT＃: RR202705
- TrueORF®
Brf1 (Myc-DDK-tagged ORF) - Rat BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (Brf1), (10 ug)
"NM_001106761" in other vectors (3)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202705 representing NM_001106761
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGCCGCGTCTGCCGCGGTTGCGGCGGCACGGACATCGAGTTGGATGCGGCGCGCGGCGACGCGG TGTGCACGGGCTGCGGTTCGGTGCTCGAGGACAACATCATCGTGTCCGAGGTGCAGTTCGTGGAGAACAG CGGCGGCGGCTCGTCGGCAGTCGGCCAGTTCGTGTCGCTGGACGGTGCGGGGAAAACCCCAACCCTAGGC GGCGGCTTTCATGTGAACTTGGGAAAGGAATCAAGAGCACAGACCCTACAGAATGGTATTCTCTACTTCA TCCTTGCTGACCATGGGGTGGGACAGGATGCTGGGGTGGGGGCGGCGCCACATCCACCACCTGGGGAGCC AGTTGCAACTGAACCAGCACTGCCTGGACACAGCCTTCAACTTCTTCAAGATGGCTGTAAGCAAGCATCT TACCCGAGGACGGAAAATGGCCCATGTGATTGCTGCCTGCCTCTACTTGACATGCTCCTCGACCTCAGCG ACCTGCTCCAGGTTAACGTGTATGTGCTGGGAAAGACCTTCCTGCTCTTGGCGAGGGAGCTTTGCATCAA CGCACCAGCTATAGACCCATGCTTGTACATTCCACGATTTGCGCATCTACTGGAATTTGGGGAGAAGAAC CATGAGGTATCCATGACGGCCCTGAGGCTTCTGCAGAGGATGAAAAGGGATTGGATGCACACAGGCCGGC GCCCCTCTGGCCTCTGTGGAGCAGCACTTCTGGTTGCAGCCAGAATGCATGACTTCCGGAGAACCGTCAA AGAAGTCATCAGCGTGGTCAAAGTATGTGAGTCCACATTGCGGAAGAGGCTCACAGAATTTGAAGACACT CCAACCAGTCAGCTCACTATTGACGAGTTTATGAAGATTGACCTGGAGGAGGAGTGTGACCCGCCCTCAT ACACCGCTGGACAGAGGAAGCTGCGCATGAAGCAGCTGGAACAGGTCCTGTCCAAAAAGCTAGAAGAAGT TGAAGGTGAGATATCCACTTACCAAGATGCCATTGAAATTGAGCTAGAAAACAGTCGGCCAAAGGCTAAA GGGGCTCTCGCCAACTTGTCAAAGGATGGCTCCGGTGAGGACGCCACATCCAGCCCGTTCTGTGAGGAAG ACACTGAGGATGAGGAGCTCGAGGCTGCTGCCAGCCACATGAACAAGGACTTTTACCGAGAGCTCCTGGG GGCTGATGGCGGCTCGGAGGCAGCAGAAGACGCTGATGGGGGCAGCAGACCCCTCGCCCTGGAGTCCCTG CTTGGTCCCCTGCCCACAGCAGCCAGCCTGGGTATCTCAGACTCCATCCGAGAGTGCATCTCCTCCCCCA GTGGAGACCCCAAAGACTCTTCAGGGGATGGGGAGCTGGACCTCAGCGGCATCGATGACCTTGAGATTGA CAGATACATCCTGAACGAGTCAGAAGCCCGGGTAAAGGCTGAGTTGTGGATGCGGGAGAATGCTGAGTAC CTGCGGGAACAGAAAGAGAAAGAAGCAAGAATAGCGAAGGAGAAGGAGCTTGGCATCTATAAGGAGCACA AGCCCAAGAAGTCTTGCAAACGCCGGGAGCCAATCCTAGCCAGCACAGCCGGGGAGGCCATTGAGAAGAT GCTGGAACAGAAGAAAATCTCCAGCAAGATCAACTACAGTGTGCTGCGGGACCTTAACAGCAAGGGTGGG GGCAGCCCGCCCAGGGACGACTCACAGCCTCCAGAGCACACCAGCACCAAGAAGCTGTCACGGAGGAAAA GAGCAGCTACCAGGAACAGTGCTGATCCTGGGACAAGCATGGGGAAGAGGTTGAGGCCTTTGGTGTCTGC TCAGCCCGCTAAGAAGGCAGCTGTGGGAGAGGCCTTGCTCTTAAATTCCCCTGCCTTGGGAGCTGAGCCT GTCAAGCCATCGGCAGTCTTGGTGGAGAGTGGCCCTGTGTCATACCATCCTGAGGAGGATGCAGATGAGG AGGACGCCGAGGAAGAGGATGGAGAGCCCTGCGTCAGTGCTCTGCAGATGATGGGAGGCAATGATTATGG CTGTGATGGTGACGAGGATGATGGCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202705 representing NM_001106761
Red=Cloning site Green=Tags(s) MTGRVCRGCGGTDIELDAARGDAVCTGCGSVLEDNIIVSEVQFVENSGGGSSAVGQFVSLDGAGKTPTLG GGFHVNLGKESRAQTLQNGILYFILADHGVGQDAGVGAAPHPPPGEPVATEPALPGHSLQLLQDGCKQAS YPRTENGPCDCCLPLLDMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGEKN HEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDT PTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISTYQDAIEIELENSRPKAK GALANLSKDGSGEDATSSPFCEEDTEDEELEAAASHMNKDFYRELLGADGGSEAAEDADGGSRPLALESL LGPLPTAASLGISDSIRECISSPSGDPKDSSGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEY LREQKEKEARIAKEKELGIYKEHKPKKSCKRREPILASTAGEAIEKMLEQKKISSKINYSVLRDLNSKGG GSPPRDDSQPPEHTSTKKLSRRKRAATRNSADPGTSMGKRLRPLVSAQPAKKAAVGEALLLNSPALGAEP VKPSAVLVESGPVSYHPEEDADEEDAEEEDGEPCVSALQMMGGNDYGCDGDEDDGY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106761 |
| ORF Size | 2058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106761.1, NP_001100231.1 |
| RefSeq Size | 2380 bp |
| RefSeq ORF | 2061 bp |
| Locus ID | 299347 |
| MW | 74.1 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202705 | Brf1 (untagged ORF) - Rat BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (Brf1), (10 ug) |
CNY 8,840.00 |
|
| RR202705L3 | Lenti ORF clone of Brf1 (Myc-DDK-tagged ORF) - Rat BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (Brf1), (10 ug) |
CNY 10,640.00 |
|
| RR202705L4 | Lenti ORF clone of Brf1 (mGFP-tagged ORF) - Rat BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (Brf1), (10 ug) |
CNY 11,690.00 |
