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Ufl1 (NM_001126279) Rat Tagged ORF Clone
CAT＃: RR202893
- TrueORF®
Ufl1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 1810074P20 (RGD1309308), (10 ug)
"NM_001126279" in other vectors (3)
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CNY 10,170.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Maxer; RGD1309308 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202893 representing NM_001126279
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCCTGGGAGGAGATCCGGCGTCTGGCGGCTGACTTCCAGCGGGCGCAGTTTGCTGAGTCCA CGCAAAGGCTGTCAGAGCGGAACTGCATTGAGATTGTGAATAAATTGATCTCTCAGAAACAGCTGGAAGT AGTTCATACGCTTGATGGGAAGGAATATATCACTCCAGCCCAGATCAGTAAAGAAATGAGAGATGAGCTA CATGTCCGAGGTGGTCGAGTAAACATTGTCGACTTACAACAGGTTATTAACGTGGACCTGACTCATATTG AAAATAGAGTCGGCGACATTATTAAGTCGGAGAAGCATGTGCAGATGGTACTGGGACAGCTGGTCGATGA GAACTATCTGGATCGGTTATCAGAAGAGGTGAATGATAAATTGCAAGAAAGTGGTCAAGTAACCGTCTCA GAGCTGTGTAAAACCTATGACCTTCCTGGGGATTTTCTGACACAGGCATTAACTCAGCGACTTGGCCGGA TTATCAATGGCCATCTTGACCTTGATAACAGAGGAGTGATTTTCACTGAGGCCTTTGTGGCTCGCCATAA AGCACGCATCCGTGGACTCTTCAGTGCTATTACCCGGCCTACAGCAGTGAACTCTTTGGTTTCAAAATAT GGATTTCAGGAACAGCTCCTTTACTCTGTGCTTGAGGAACTTGTTAGCACTGGCCGCCTACGAGGCACGG TGGTTGGCGGGCGACAGGACAAAGCAGTGTTTGTCCCTGACATCTATTCCAGGACACAGAGTACTTGGGT GGATTCCTTCTTCAGGCAGAACGGCTATCTAGAATTTGACGCTTTATCCAGACTTGGAATCCCAGATGCT GTGAACTATATAAAGAAAAGGTATAAGAATACCCCACTCTTGTTTTTGAAGGCAACTTGTGTTGGCCAAG GGCTCGTGGACCAGGTGGAAGCATCGGTAGAGGAGGCCATCAGCTCAGGAACATGGGTTGACGTTTCGCC TCTGCTGCCCAGTTCTTTGTCAGTTGAAGATGCTGCTATGCTGCTTCAGCAGGTGATGAGACCATTTGGC AAGCATGCGTCAGCCACAGTCTTTAGTGACACTGTTGTGGTCAGCGAGAAATTCATCAATGACTGTACAA AACTGTTCAGCGAACGAATGCACCAAAAAGCTGAAAAGGAAATGAAAAATAATCCTGTGCATTTAATCAC AGAAGAAGATCTGAAACAAATATCCATTTTAGAAAGTGTTAATACAAATAAAAAGGATAAAAAAGATGAA CGCAGGAAGAAAGCAACAGAGGGTTCTGGAAGTGTAAGAGGCGGAGGAGGGGGCAATGCCAGAGAATACA AGATTAAGAAAGTCAAGAAGAAGGGAAGAAAAGACGAGGACAGTGATGATGAGTCTCAGTCATCGCATGC TGGAAAGAAGAAGCCAGACATCACCTTCATGTTCCAGGATGAAATTGAAGGTTGCTTAAGGAAACACATT CCAGATGCCCCTGAGGAGTTTATTTCAGAACTTGCAGAACACTTAATAAAGCCTCTGAATAAAATGTATC TTGAGGTGGTACGTTCAGTCTTCATGTCGTCAACATCTGCTTCTGGAACTGGCAGAAAACGCACGATCAA GGACTTACAAGAAGAGGTTTCAAATCTATACAATAATATCAGATTATTTGAAAAGGGGATGAAATATTTT GCAGATGACACACAGACTGCTCTGACCAAGCACTTGCTGAAGACAGTGTGTACCGATATCACTAACCTCA TGTTCAACTTCCTAGCCTCAGACTTGATGATGGCGGTGGAGGACCCTGCAACCATTACAAGTGATATGAG AAAAAAAATTTTAAGCAAATTGACAGAAGAAACTAAAGTAGCTCTCACAAAACTCCACAACTCCCTGAAT GAAAAAAGCATAGAAGACTTCCTTTCTTGTCTGGATTCTGCAACAGAAGCTTGTGACATTATGGTGAAAA AGGGAGACAAAAAAAGGGAAAGGCAAATCCTTTTCCAGCATCGGCAGGCTCTGGCTGACCAGCTGAAGGT CACAGAAGACCCTGCTCTGATTTTACATCTCACATCAGTCCTGTTATTTCAGTTCTCAACCCACAGCATG CTCCATGCACCTGGAAGATGTGTCCCACAGATCATCGCCTTTCTTCACAACAAAATCCCAGAGGATCAAC ATACTCTCTTGGTAAAATATCAAGGATTGGTTGTGAAACAGTTAGTCAGTCAAAATAAGAAGAGTGGACA GGGAGAGGATCCGTCAAGCGATGACTTAGACAAGGAACAACATGACGTCACCAATACTACTCGGAAGGAG CTTCAGGAACTGTCTTTATCCATTAAGGACCTTGTTCTGAAGCCCAGGAAATCATCTGTGACAGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202893 representing NM_001126279
Red=Cloning site Green=Tags(s) MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDEL HVRGGRVNIVDLQQVINVDLTHIENRVGDIIKSEKHVQMVLGQLVDENYLDRLSEEVNDKLQESGQVTVS ELCKTYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLVSKY GFQEQLLYSVLEELVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDA VNYIKKRYKNTPLLFLKATCVGQGLVDQVEASVEEAISSGTWVDVSPLLPSSLSVEDAAMLLQQVMRPFG KHASATVFSDTVVVSEKFINDCTKLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTNKKDKKDE RRKKATEGSGSVRGGGGGNAREYKIKKVKKKGRKDEDSDDESQSSHAGKKKPDITFMFQDEIEGCLRKHI PDAPEEFISELAEHLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYF ADDTQTALTKHLLKTVCTDITNLMFNFLASDLMMAVEDPATITSDMRKKILSKLTEETKVALTKLHNSLN EKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALADQLKVTEDPALILHLTSVLLFQFSTHSM LHAPGRCVPQIIAFLHNKIPEDQHTLLVKYQGLVVKQLVSQNKKSGQGEDPSSDDLDKEQHDVTNTTRKE LQELSLSIKDLVLKPRKSSVTEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001126279 |
| ORF Size | 2379 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001126279.1, NP_001119751.1 |
| RefSeq Size | 2766 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 313115 |
| UniProt ID | B2GV24 |
| MW | 89.6 kDa |
| Gene Summary | E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling. May also play a role in nuclear receptor-mediated transcription through TRIP4 ufmylation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. Anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202893 | Ufl1 (untagged ORF) - Rat similar to RIKEN cDNA 1810074P20 (RGD1309308), (10 ug) |
CNY 10,170.00 |
|
| RR202893L3 | Lenti ORF clone of Ufl1 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 1810074P20 (RGD1309308), (10 ug) |
CNY 11,970.00 |
|
| RR202893L4 | Lenti ORF clone of Ufl1 (mGFP-tagged ORF) - Rat similar to RIKEN cDNA 1810074P20 (RGD1309308), (10 ug) |
CNY 13,210.00 |
