Ralgds (NM_019250) Rat Tagged ORF Clone
CAT#: RR203249
- TrueORF®
Ralgds (Myc-DDK-tagged ORF) - Rat ral guanine nucleotide dissociation stimulator (Ralgds), (10 ug)
"NM_019250" in other vectors (3)
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CNY 11,590.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Rgds |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR203249 representing NM_019250
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAGCGCATGTGGGCCGAGGCGTCCGGGCCCATAGGGGGCGCCGAGCCGTTGTTTCCCGGCTCCC GGCGCAGCCGCAGCGTGTGGGACGCCGTGCGTCTGGAGGTGGGCGTCCCTGACAGCTGTCCAGTGGTGCT GCACAGCTTCACGCAGCTCGACCCCGATCTGCCGCGCCTGGAGAGCTCTACGCAGGAGATTGGGGAGGAG CTGATCAACGGGGTCATCTACTCCATCTCCCTGCGCAAGGTTCAGCTACACCCAGGAGCCACCAAGGGCC AGCGCTGGCTAGGGTGCGAGAATGAGTCAGCTCTGAACCTCTACGAGACTTGCAAGGTGCGCACGGTGAA GGCCGGTACTCTGGAGAAGCTGGTGGAACACCTGGTGCCTGCCTTCCAGGGCAGTGACCTTTCCTACGTC ACCGTATTCCTGTGCACCTACAGAGCCTTCACAACCACTCAGCAGGTGCTAGACCTGCTGTTCAAAAGAT ATGGCTGCATCCTTCCCTACTCCAGTGAGGACGGCGGACCACAGGACCAACTCAAAAATGCCATCTCTTC CATCCTGGGCACCTGGCTGGACCAATACTCCGAGGACTTCTGTCAACCCCCGGACTTTCCCTGCCTCAAG CAGCTGGTGGCTTATGTGCAGCTCAACATGCCTGGCTCAGACCTGGAGCGCCGTGCTCACCTTCTCCTGG CCCAGCTGGAGGACCTGGAGCCCAGTGAGGTTGAGCCTGAGGCCCTGTCCCCAGCTCCAGTGCTGTCTCT GAAACCAGCTTCACAGCTAGAACCAGCTCCCGCCCTGCTTCTGACGCCCAGCCGAGCTGTGGCATCCACT CCAGTCCGAGAGCCAGCCCCGGTGCCAGTACTGGCATCCAGCCCGGTGGTGGCGCCAGCCTCTGAGCTAG AACCAGCTCTGGAGCCGCCCCTAGACCCTGAGCCAACCCTCGCACCTGCTCCAGAGCTGGACCCCACTGT CTCACAGAGCCTCCACCTCGAGCCCGCTCCCGTGCCCGCTCCTGCATTAGAGCCTTCCTGGCCTCTGCCT GAAACCACGGAGAACGGACTGTGTGCAAAGCCTCACCTACTGCTGTTCCCTCCAGACTTGGTGGCTGAAC AGTTTACGCTGATGGACGCAGAGCTATTCAAGAAAGTTGTGCCCTACCACTGCCTGGGCTCCATCTGGTC CCAGCGGGACAAGAAGGGCAAGGAGCACCTCGCGCCCACCATCCGTGCCACTGTCGCCCAGTTCAACAAC GTGGCCAACTGTGTCATCACCACCTGCCTTGGGGACCAGAGTATGAAAGCTTCGGACAGGGCCCGGGTGG TGGAACACTGGATCGAGGTGGCCAGGGAGTGCAGGGTGCTCAAGAATTTCTCCTCCCTCTACGCCATCCT CTCTGCCCTACAGAGCAATGCCATCCACCGCCTAAAGAAGACATGGGAAGAGGTCTCCAGGGGCAGCTTT CGAGTGTTCCAGAAACTGTCAGAAATCTTCTCTGATGAGAACAACTACTCCTTGAGCAGAGAGCTGCTCA TCAAGGAAGGAACCTCCAAGTTTGCCACGCTGGAGATGAACCCTAGGAGAACCCAGAGGCGGCAGAAGGA GACAGGTGTCATCCAGGGCACTGTCCCCTACCTGGGCACATTCCTCACTGACCTGGTGATGCTGGACACA GCCATGAAGGACTATCTATATGGGAGACTGATCAACTTTGAAAAGAGGAGGAAGGAGTTCGAAGTCATCG CCCAGATCAAGTTGCTCCAGTCGGCCTGCAACAACTACAGCATTGTTCCCGAGGAGCACTTTGGAGCCTG GTTCCGAGCCATGGGACGGCTCAGCGAGGCTGAGAGCTACAACCTGTCGTGTGAGCTGGAGCCCCCGTCT GAGTCAGCCAGCAACACCCTGAGGAGCAAGAAAAGCACAGCTATCGTCAAGCGCTGGAGCGACCGCCAGG CTCCCAGCACGGAGCTCAGCACCAGTAGCAGTGCACACTCCAAGTCATGTGACCAGCTCCGGTGCAGCCC CTACCTCAGCAGCGGGGACATCACGGACGCACTCAGTGTGCACTCAGCTGGTTCATCCAGCTCTGACGTG GAGGAGATCAACATGAGCTTTGTTCCAGAGTCTCCTGATGGCCAGGAAAAGAAGTTCTGGGAGTCAGCCT CCCAGTCGTCCCCAGAGACCTCTGGCATCAGCTCGGCCTCCAGCAGCACCTCCTCTTCGTCAGCCTCTAC CACACCGGTGTCTACCACACGCACCCACAAGCGCTCTGTCTCAGGGGTCTGCAGCTACAGCTCCTCACTG CCACTCTACAACCAGCAGGTGGGCGACTGCTGCATCATCAGGGTCAGCCTGGATGTGGACAACGGCAACA TGTACAAGAGCATCCTGGTGACCAGCCAGGATAAGGCTCCGACTGTCATCCGGAAAGCCATGGACAAACA CAACCTAGATGAGGACGAGCCCGAGGACTATGAGCTGCTGCAGATCATCTCAGAGGACCACAAGCTGAAA ATTCCAGAGAACGCCAATGTGTTCTATGCCATGAACTCTGCCGCCAACTATGACTTCATCCTAAAGAAGC GGGCCTTCACTAAGGGGGCTAAAGTCAAGCATGGAGCCAGCTCCACTCTCCCTCGAATGAAGCAGAAGGG ACTCAGGATTGCCAGAGGCATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203249 representing NM_019250
Red=Cloning site Green=Tags(s) MVQRMWAEASGPIGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCPVVLHSFTQLDPDLPRLESSTQEIGEE LINGVIYSISLRKVQLHPGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV TVFLCTYRAFTTTQQVLDLLFKRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK QLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEVEPEALSPAPVLSLKPASQLEPAPALLLTPSRAVAST PVREPAPVPVLASSPVVAPASELEPALEPPLDPEPTLAPAPELDPTVSQSLHLEPAPVPAPALEPSWPLP ETTENGLCAKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVAQFNN VANCVITTCLGDQSMKASDRARVVEHWIEVARECRVLKNFSSLYAILSALQSNAIHRLKKTWEEVSRGSF RVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRTQRRQKETGVIQGTVPYLGTFLTDLVMLDT AMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIVPEEHFGAWFRAMGRLSEAESYNLSCELEPPS ESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLSSGDITDALSVHSAGSSSSDV EEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKRSVSGVCSYSSSL PLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYELLQIISEDHKLK IPENANVFYAMNSAANYDFILKKRAFTKGAKVKHGASSTLPRMKQKGLRIARGIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019250 |
ORF Size | 2685 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019250.2, NP_062123.2 |
RefSeq Size | 3684 bp |
RefSeq ORF | 2688 bp |
Locus ID | 29622 |
UniProt ID | Q03386 |
MW | 98.8 kDa |
Gene Summary | facilitates conversion of Ras-related GTPase (Ral) to its GTP-bound form; may be involved in cardiac hypertrophy [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN203249 | Ralgds (untagged ORF) - Rat ral guanine nucleotide dissociation stimulator (Ralgds), (10 ug) |
CNY 11,590.00 |
|
RR203249L3 | Lenti ORF clone of Ralgds (Myc-DDK-tagged ORF) - Rat ral guanine nucleotide dissociation stimulator (Ralgds), (10 ug) |
CNY 13,210.00 |
|
RR203249L4 | Lenti ORF clone of Ralgds (mGFP-tagged ORF) - Rat ral guanine nucleotide dissociation stimulator (Ralgds), (10 ug) |
CNY 14,540.00 |