Sbno1 (NM_001107138) Rat Tagged ORF Clone

CAT#: RR203270

  • TrueORF®

Sbno1 (Myc-DDK-tagged ORF) - Rat strawberry notch homolog 1 (Drosophila) (Sbno1), (10 ug)



  "NM_001107138" in other vectors (1)

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CNY 19,950.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203270 representing NM_001107138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGAACCAGGGCAAGATTTACTACTTGCGGCCTTGAGCGAGAGTGGGATTAGTCCGAATGACCTCT
TTGATGTTGATGGTGGAGATGCTGGACTTGCCACCCCTACTCCCCCTTCAGTTCAACAGTCGGTGCCACT
TAGTGCATTAGAACTAGGTTTGGAGACAGAAGCAGCAATTCCTGTTAAACAAGAACCCGAGATGATGTCT
ACTCCGGCACTGTTAAATGTGAGGCAGCAGCCTCCATCCACTACAACATTTGTGCTGAATCAAATCAATC
AACTTCCAACCTTGGGATCTACAATTGTAATGACTAAAACACCACCTGCAACAACGAATAGGCAAACCAT
CACTTTGACTAAGTTCATCCAGACCACTGCAAACACACGCCCTTCAGTTTCAGCACCAGCAGTACGGAAT
GCCATCCCCCCTGCTCCCGCAAAAGACCAAGTTCAGCTAAAGGATTTACTCAAAAATAATAGTTTAAATG
AACTCATGAAACTGAAGCCACCTGCCAATATTGCTCAGCCAGTTGCAACTGCGGCTACTGATGTCAGCAA
TGGCGCAGTTAAAAAGGAGTCTTCTAATAAAGAAGTTGCAAGGGTATGGATAAATGACATGAAGATGAGA
AGCTTTTCCCCCACTATGAAGGTTCCTGTTGTGAGAGAAGAAGATGAACCAGAAGAAGAAGATGAGGAAG
AAATGGGTCATGCAGAAACCTATGCAGAGTATATGCCAATAAAATTAAAAATTGGCCTGCGTCACCCTGA
TGCGGTGGTGGAGACAAGCTCACTGTCCAGCGTCACTCCTCCTGATGTTTGGTACAAAACGTCCATCTCT
GAGGAGACCATCGATAATGGCTGGCTCTCTGCATTGCAGCTTGAGGCAGTCACCTATGCGGCCCAGCAAC
ATGAAACATTCCTTCCTAATGGAGACCGTGCTGGCTTCTTAATAGGCGATGGTGCTGGTGTGGGCAAAGG
CCGGACGATAGCAGGGATCATATATGAAAACTATTTGTTGAGTAGAAAAAGAGCATTGTGGTTTAGTGTA
TCAAATGACTTAAAATATGATGCTGAAAGAGATTTAAGGGATATTGGAGCAAAAAACATTTTGGTCCATT
CATTAAATAAGTTTAAATATGGAAAGATTTCTTCTAAACACAATGGGAGTGTGAAAAAGGGTGTTATTTT
TGCTACCTATTCTTCCCTTATTGGTGAAAGCCAGTCTGGAGGTAAATATAAGACCAGGTTAAAACAGCTT
CTGCACTGGTGTGGTGACGACTTCGATGGAGTGATAGTGTTTGATGAATGTCACAAAGCAAAAAACTTAT
GTCCTGTTGGTTCTTCAAAGCCAACCAAGACAGGCTTGGCTGTTTTAGAACTTCAAAACAAATTGCCAAA
AGCCAGAGTCGTGTATGCCAGTGCCACCGGTGCTTCTGAACCACGCAACATGGCCTATATGAACCGTCTT
GGAATATGGGGTGAGGGAACTCCATTCAGAGAATTCAGTGATTTTATCCAAGCTGTGGAACGCAGAGGTG
TTGGTGCCATGGAAATTGTTGCTATGGATATGAAGCTTAGAGGAATGTACATTGCACGACAGCTGAGCTT
TACTGGAGTGACCTTCAAAATTGAGGAAGTTCTTCTTTCTCAAAGCTACGTTAAAATGTATAACAAAGCA
GTCAAACTGTGGGTTATCGCTAGAGAGCGATTTCAGCAAGCTGCAGATTTGATTGATGCTGAGCAGAGAA
TGAAGAAATCTATGTGGGGTCAGTTCTGGTCTGCTCACCAGAGGTTTTTTAAGTACCTGTGTATAGCGTC
CAAAGTGAAGAGGGTGGTCCAGCTAGCCCGGGAAGAAATCAAGAATGGGAAGTGTGTTGTAATTGGTCTG
CAGTCCACAGGAGAAGCCAGAACTTTAGAGGCCTTGGAAGAGGGTGGAGGAGAACTAAATGATTTTGTTT
CAACTGCCAAAGGGGTATTGCAGTCACTGATTGAAAAACACTTCCCTGCTCCAGACAGGAAGAAACTCTA
TAGTTTGCTAGGAATCGACTTGACAGCCCCAAGTAACAACAGTTCACCAAGAGATAGTCCTTGTAAAGAG
AATAAAATAAAGAAGCGGAAAGGTGAAGAAATTACTCGAGAAGCCAAAAAAGCACGAAAAATTGGTGGCC
TTACTGGAAGCAGCTCTGACGACAGTGGGAGTGAATCTGATGTCTCTGATAATGAAGAAAGCGACTATGA
GAGCTCTAAAAACATGAGCTCTGGTGATGACGATGATTTCAATCCCTTCAGAGATGAGTCCAGCGAAGAC
AACGAGGACGATCCATGGTTAATTAGGAAAGATCATAAGAAAAGCAAAGATAAAAAAAAGAAGAAAAGTA
TCGATCCAGATTCCATTCAAAGTGCCTTGTTAGCATCTGGTCTTGGATCAAAGCGGCCCAGTTTCTCCTC
AGCACCAGTCATCTCACCTGCTTCCAACAGTGCACCAGCCAACAGTAACAGCAACAGTAACAGCAGCCTC
ATAACGAGCCAAGATGCTGTTGAAAGGGCACAGCAGATGAAGAAAGACCTCCTTGATAAGCTGGAAAAGT
TAGCTGAGGACCTCCCGCCTAATACCCTGGATGAGCTTATCGATGAGCTCGGTGGTCCTGAAAATGTGGC
TGAGATGACTGGCCGCAAGGGGCGGGTGGTGAGCAATGATGATGGAAGCATATCCTACGAGTCAAGGTCT
GAACTTGATGTGCCTGTGGAGATTCTGAACATCACGGAGAAGCAGAGGTTCATGGATGGAGACAAGAATA
TTGCTATTATCTCAGAAGCTGCCAGCTCAGGTATTTCATTACAAGCAGATCGGAGAGCTAAGAATCAAAG
GAGAAGAGTTCATATGACTTTAGAGTTACCCTGGAGTGCTGATCGGGCCATTCAGCAGTTCGGAAGAACA
CACAGGTCAAACCAAGTGACTGCTCCAGAGTATGTTTTTCTGATTTCGGAGCTGGCTGGAGAACAGAGGT
TTGCGTCTATTGTTGCTAAGAGACTTGAGAGTCTGGGGGCACTTACACATGGCGACAGAAGAGCAACAGA
ATCTAGAGATCTCAGCAGGTTCAACTTTGACAACAAGTATGGAAGAAATGCTTTAGAAATTGTTATGAAG
TCCATTGTAAACTTGGATTCTCCTATGGTATCACCACCTCCAGACTACCCTGGAGAATTCTTTAAAGATG
TTCGGCAAGGACTGATAGGAGTGGGCTTGATTAATGTAGAAGATCGCTCAGGAATTCTTACTCTTGATAA
AGACTATAATAATATAGGAAAATTCTTAAACAGAATTCTGGGCATGGAGGTGCATCAGCAGAATGCGTTG
TTTCAGTATTTTGCAGACACACTCACTGCAGTTGTTCAGAATGCCAAGAAGAATGGGAGATACGACATGG
GAATCCTAGATCTGGGTTCTGGAGATGAAAAGGTGAGGAAGAGTGATGTCAAGAAGTTTCTGACTCCAGG
ATACTCAACCTCTGGCCATGTAGAGTTATATACAATTAGTGTGGAGAGGGGAATGTCCTGGGATGAAGCA
ACCAAGATTTGGGCCGAGCTGACAGGACCCGACGATGGCTTTTACTTATCGTTGCAATGTTCGTTTTGGA
GGAGAATAGGCCTTAGGAAGGCCTATGTTAACTTGACTGCTGTTGACTGTGCCGCTGGCGTGGCAATTGC
AAAAAAGCAAGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR203270 representing NM_001107138
Red=Cloning site Green=Tags(s)

MVEPGQDLLLAALSESGISPNDLFDVDGGDAGLATPTPPSVQQSVPLSALELGLETEAAIPVKQEPEMMS
TPALLNVRQQPPSTTTFVLNQINQLPTLGSTIVMTKTPPATTNRQTITLTKFIQTTANTRPSVSAPAVRN
AIPPAPAKDQVQLKDLLKNNSLNELMKLKPPANIAQPVATAATDVSNGAVKKESSNKEVARVWINDMKMR
SFSPTMKVPVVREEDEPEEEDEEEMGHAETYAEYMPIKLKIGLRHPDAVVETSSLSSVTPPDVWYKTSIS
EETIDNGWLSALQLEAVTYAAQQHETFLPNGDRAGFLIGDGAGVGKGRTIAGIIYENYLLSRKRALWFSV
SNDLKYDAERDLRDIGAKNILVHSLNKFKYGKISSKHNGSVKKGVIFATYSSLIGESQSGGKYKTRLKQL
LHWCGDDFDGVIVFDECHKAKNLCPVGSSKPTKTGLAVLELQNKLPKARVVYASATGASEPRNMAYMNRL
GIWGEGTPFREFSDFIQAVERRGVGAMEIVAMDMKLRGMYIARQLSFTGVTFKIEEVLLSQSYVKMYNKA
VKLWVIARERFQQAADLIDAEQRMKKSMWGQFWSAHQRFFKYLCIASKVKRVVQLAREEIKNGKCVVIGL
QSTGEARTLEALEEGGGELNDFVSTAKGVLQSLIEKHFPAPDRKKLYSLLGIDLTAPSNNSSPRDSPCKE
NKIKKRKGEEITREAKKARKIGGLTGSSSDDSGSESDVSDNEESDYESSKNMSSGDDDDFNPFRDESSED
NEDDPWLIRKDHKKSKDKKKKKSIDPDSIQSALLASGLGSKRPSFSSAPVISPASNSAPANSNSNSNSSL
ITSQDAVERAQQMKKDLLDKLEKLAEDLPPNTLDELIDELGGPENVAEMTGRKGRVVSNDDGSISYESRS
ELDVPVEILNITEKQRFMDGDKNIAIISEAASSGISLQADRRAKNQRRRVHMTLELPWSADRAIQQFGRT
HRSNQVTAPEYVFLISELAGEQRFASIVAKRLESLGALTHGDRRATESRDLSRFNFDNKYGRNALEIVMK
SIVNLDSPMVSPPPDYPGEFFKDVRQGLIGVGLINVEDRSGILTLDKDYNNIGKFLNRILGMEVHQQNAL
FQYFADTLTAVVQNAKKNGRYDMGILDLGSGDEKVRKSDVKKFLTPGYSTSGHVELYTISVERGMSWDEA
TKIWAELTGPDDGFYLSLQCSFWRRIGLRKAYVNLTAVDCAAGVAIAKKQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001107138
ORF Size 3723 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001107138.1, NP_001100608.1
RefSeq Size 5355 bp
RefSeq ORF 3726 bp
Locus ID 304470
MW 136.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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