Asah2 (NM_053646) Rat Tagged ORF Clone

CAT＃: RR203862

  • TrueORF®

Asah2 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)

Datasheet
SDS
Plasmid Map

  "NM_053646" in other vectors (3)

Need custom modification / cloning service?
Get a free quote

CNY 9,790.00

货期*
6周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00

添加到购物车
CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车
TA592569S
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

添加到购物车

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR203862 representing NM_053646
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAGCGAACCTTCTCCTCCTTGGAGGCGTTCCTCATTTTCCTTCTGGTAATGATGACGGCCATCA
CAGTGGCCCTTCTCACCCTCTTGTTCGTCACCAGTGGGACCATCGAAAACCACAAAGATTCAGGGAATCA
CTGGGTTTCAACCACCCAGGGCCCCACAACCACCCAGTCCTCTCCAACCACACAAACTCCAACCACACAA
ACTCCAGACCTCCCTCCCTCTCAGAACTTCAGTGGCTACTACATTGGTGTTGGGCGAGCTGACTGCACCG
GACAAGTGTCAGATATCAATTTGATGGGCTATGGCAAAAATGGCCAGAACGCACAGGGTCTCCTCACCAG
GCTGTTCAGCCGCGCTTTTATCTTGGCGGATCCAGATGGGTCAAATCGAATGGCATTTGTGAGCGTGGAA
CTATGCATGATTTCCCAACGACTCAGGTTGGAGGTCTTGAAGAGACTACAGAGTAAATATGGCTCTCTGT
ATCGAAGAGACAACGTTATCCTGAGTGCCACTCACACTCACTCTGGCCCAGCAGGATTTTTCCAATATAC
ACTCTATATACTTGCCAGCGAGGGATTCAGCAACCGGACCTTTCAGTACATAGTCTCTGGGATCGTGAAG
AGCATTGATATAGCACACACAAATCTTAAACCGGGCAAAGTCCTTATCAACAAAGGAAATGTTGCTAATG
TGCAGATCAACCGCAGTCCCTCCTCTTACCTTCAGAATCCACCTTCGGAGAGAGCAAGGTATTCTTCCGA
CACGGACAAGGAAATGGTCGTCTTGAAACTGGTGGATTTGAATGGAGAAGACTTGGGCCTTATCAGCTGG
TTTGCCGTCCACCCCGTGAGCATGAACAACAGTAACCACCTCGTCAACAGTGACAATATGGGCTACGCGG
CTTACCTTTTCGAGCAAGAAAAGAACAGAGGCTATCTGCCTGGACAGGGACCATTCGTAGCAGGCTTTGC
TTCATCAAATCTCGGAGACGTGTCGCCCAACATTCTTGGCCCACATTGTGTCAACACAGGGGAGTCTTGT
GACAACGACAAAAGCACCTGTCCCAGTGGTGGGCCTAGCATGTGCATGGCCAGTGGACCCGGACAAGATA
TGTTTGAGAGCACACACATTATAGGACGGGTCATCTATCAGAAAGCCAAGGAGCTGCATGCCTCTGCCTC
CCAGGAAGTGACCGGCCCAGTGCTCACAGCTCACCAGTGGGTGAACATGACGGATGTGAGCGTCCAACTC
AATGCCACACACACAGTGAAGACGTGTAAAGCCGCCCTGGGCTACAGTTTTGCCGCAGGCACAATTGATG
GAGTTTCGGGCCTCAATATTACACAGGGAACAACAGAAGGGAATCTATTTTGGGACACTCTTCGGGACCA
GCTCTTGGGAAAACCATCTGAAGAAATCATAGAATGCCAGAAACCTAAACCAATCCTAATCCACACGGGA
GAGCTGACAAAACCTCATCCTTGGCAACCAGATATTGTTGATATTCAGATCGTTACTCTTGGTTCGTTGG
CCATAGCTGCTATCCCTGGGGAATTTACAACCATGTCAGGTCGAAGACTTCGTGAGGCAGTTAAAAAAGA
ATTTGCACTTTATGGGATGAAGGATATGACTGTCGTTATCGCAGGGCTGAGCAATGTTTATACCCATTAC
ATTACCACATATGAAGAATACCAGGCTCAGCGGTATGAGGCAGCATCTACGATTTATGGACCACACACCC
TGTCTGCGTACATCCAGCTCTTCAGAGCCCTTGCTAAGGCCATTGCTACGGATACAGTAGCCAACATGAG
CAGCGGTCCTGAGCCTCCATTCTTCAAAAACCTGATAGGTTCACTTATTCCTAATATTGCGGATAGAGCA
CCAATAGGCAAACAATTTGGGGATGTCCTGCAGCCAGCAAAACCTGAATACAGAGTGGGAGAAGTGGTTG
AAGTTGTCTTTGTAGGCGCTAACCCAAAGAATTCAGCAGAAAACCAGACCCATCAAACCTTCCTCACTGT
GGAGAAATACGAGGACTCTGTAGCCAACTGGCAGATAATGCATAACGATGCCTCCTGGGAGACGAGATTT
TATTGGCACAAGGGAGTACTGGGTCTGAGCAATGCAACAATACATTGGCATATTCCAGATACTGCCCTCC
CTGGAGTCTACAGAATCAGATATTTTGGACACAATCGGAAGCAGGAACTTCTAAAGCCTGCTGTCATACT
AGCATTTGAAGGAATTTCTTCCCCTTTTGAAATTGTCACTACT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>RR203862 representing NM_053646
Red=Cloning site Green=Tags(s)

MAKRTFSSLEAFLIFLLVMMTAITVALLTLLFVTSGTIENHKDSGNHWVSTTQGPTTTQSSPTTQTPTTQ
TPDLPPSQNFSGYYIGVGRADCTGQVSDINLMGYGKNGQNAQGLLTRLFSRAFILADPDGSNRMAFVSVE
LCMISQRLRLEVLKRLQSKYGSLYRRDNVILSATHTHSGPAGFFQYTLYILASEGFSNRTFQYIVSGIVK
SIDIAHTNLKPGKVLINKGNVANVQINRSPSSYLQNPPSERARYSSDTDKEMVVLKLVDLNGEDLGLISW
FAVHPVSMNNSNHLVNSDNMGYAAYLFEQEKNRGYLPGQGPFVAGFASSNLGDVSPNILGPHCVNTGESC
DNDKSTCPSGGPSMCMASGPGQDMFESTHIIGRVIYQKAKELHASASQEVTGPVLTAHQWVNMTDVSVQL
NATHTVKTCKAALGYSFAAGTIDGVSGLNITQGTTEGNLFWDTLRDQLLGKPSEEIIECQKPKPILIHTG
ELTKPHPWQPDIVDIQIVTLGSLAIAAIPGEFTTMSGRRLREAVKKEFALYGMKDMTVVIAGLSNVYTHY
ITTYEEYQAQRYEAASTIYGPHTLSAYIQLFRALAKAIATDTVANMSSGPEPPFFKNLIGSLIPNIADRA
PIGKQFGDVLQPAKPEYRVGEVVEVVFVGANPKNSAENQTHQTFLTVEKYEDSVANWQIMHNDASWETRF
YWHKGVLGLSNATIHWHIPDTALPGVYRIRYFGHNRKQELLKPAVILAFEGISSPFEIVTT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_053646
ORF Size 2283 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053646.2, NP_446098.1
RefSeq Size 4914 bp
RefSeq ORF 2286 bp
Locus ID 114104
UniProt ID Q91XT9
MW 83.5 kDa
Gene Summary membrane-bound neutral ceramidase; hydrolyzes the N-acyl linkage of ceramide to produce sphingosine [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
RN203862 Asah2 (untagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)
    • 10 ug

CNY 9,790.00

RR203862L3 Lenti ORF clone of Asah2 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)
    • 10 ug

CNY 11,590.00

RR203862L4 Lenti ORF clone of Asah2 (mGFP-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)
    • 10 ug

CNY 12,640.00

You may also need
plasmid icon Empty Vector
protein iconDDK/Flag Antibody

其它Asah2产品

Expression Plasmids
Lentiviral Particles
CRISPR
RNAi
Proteins
Antibodies
相关产品 (8)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

    • 10 ug

CNY 5,420.00

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

    • 1 ml in 1 vial

CNY 4,090.00

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

【特别福利】购一抗正装产品，免费领10ul抗体试用装

    • 100 ul

CNY 1,050.00

RR203862L3V

Lenti ORF particles, Asah2 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), 200ul, >10^7 TU/mL

    • 200 ul

CNY 12,483.00 暂停销售

RN203862

Asah2 (untagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)

    • 10 ug

CNY 9,790.00

RR203862L4V

Lenti ORF particles, Asah2 (GFP-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), 200ul, >10^7 TU/mL

    • 200 ul

CNY 13,870.00 暂停销售

RR203862L4

Lenti ORF clone of Asah2 (mGFP-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)

    • 10 ug

CNY 12,640.00

RR203862L3

Lenti ORF clone of Asah2 (Myc-DDK-tagged ORF) - Rat N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (Asah2), (10 ug)

    • 10 ug

CNY 11,590.00

Previous
Next
Customer Reviews