Ptger1 (NM_013100) Rat Tagged ORF Clone
CAT#: RR204660
- TrueORF®
Ptger1 (Myc-DDK-tagged ORF) - Rat prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10 ug)
"NM_013100" in other vectors (3)
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CNY 5,488.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204660 representing NM_013100
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCTACGGGCTTAACCTGAGCCTAGTGGATGAGGCAACAACGTGTGTAACACCCAGGGTCCCCA ATACATCTGTGGTGCTGCCAACAGGCGGTAACGGCACATCACCAGCGCTGCCTATCTTCTCCATGACGCT GGGTGCTGTGTCCAACGTGCTGGCGCTGGCGCTGCTGGCCCAGGTTGCAGGCAGACTGCGGCGCCGCCGC TCGACTGCCACCTTCCTGTTGTTCGTCGCCAGCCTGCTTGCCATCGACCTAGCAGGCCATGTGATCCCGG GCGCCTTGGTGCTTCGCCTGTATACTGCAGGACGTGCGCCCGCTGGCGGGGCCTGTCATTTCCTGGGCGG CTGCATGGTCTTCTTTGGCCTGTGCCCACTTTTGCTTGGCTGTGGCATGGCCGTGGAGCGCTGCGTGGGT GTCACGCAGCCGCTGATCCACGCGGCGCGCGTGTCCGTAGCCCGCGCACGCCTGGCACTAGCCCTGCTGG CCGCCATGGCTTTGGCAGTGGCGCTGCTGCCACTAGTGCACGTGGGTCACTACGAGCTACAGTACCCTGG CACTTGGTGTTTCATTAGCCTTGGGCCTCCTGGAGGTTGGCGCCAGGCGTTGCTTGCGGGCCTCTTCGCC GGCCTTGGCCTGGCTGCGCTCCTTGCCGCACTAGTGTGTAATACGCTCAGCGGCCTGGCGCTCCTTCGTG CCCGCTGGAGGCGGCGTCGCTCTCGACGTTTCCGAGAGAACGCAGGTCCCGATGATCGCCGGCGCTGGGG GTCCCGTGGACTCCGCTTGGCCTCCGCCTCGTCTGCGTCATCCATCACTTCAACCACAGCTGCCCTCCGC AGCTCTCGGGGAGGCGGCTCCGCGCGCAGGGTTCACGCACACGACGTGGAAATGGTGGGCCAGCTCGTGG GCATCATGGTGGTTTCGTGCATCTGCTGGAGCCCCCTGCTGGTATTGGTGGTGTTGGCCATCGGGGGCTG GAACTCTAACTCCCTGCAGCGGCCGCTCTTTCTGGCTGTACGCCTCGCGTCGTGGAACCAGATCCTGGAC CCATGGGTGTACATCCTGCTGCGCCAGGCTATGCTGCGCCAACTTCTTCGCCTCCTACCCCTGAGGGTTA GTGCCAAGGGTGGTCCAACGGAGCTGAGCCTAACCAAGAGTGCCTGGGAGGCCAGTTCACTGCGTAGCTC CCGGCACAGTGGCTTCAGCCACTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204660 representing NM_013100
Red=Cloning site Green=Tags(s) MSPYGLNLSLVDEATTCVTPRVPNTSVVLPTGGNGTSPALPIFSMTLGAVSNVLALALLAQVAGRLRRRR STATFLLFVASLLAIDLAGHVIPGALVLRLYTAGRAPAGGACHFLGGCMVFFGLCPLLLGCGMAVERCVG VTQPLIHAARVSVARARLALALLAAMALAVALLPLVHVGHYELQYPGTWCFISLGPPGGWRQALLAGLFA GLGLAALLAALVCNTLSGLALLRARWRRRRSRRFRENAGPDDRRRWGSRGLRLASASSASSITSTTAALR SSRGGGSARRVHAHDVEMVGQLVGIMVVSCICWSPLLVLVVLAIGGWNSNSLQRPLFLAVRLASWNQILD PWVYILLRQAMLRQLLRLLPLRVSAKGGPTELSLTKSAWEASSLRSSRHSGFSHL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013100 |
ORF Size | 1215 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013100.1, NP_037232.1 |
RefSeq Size | 2216 bp |
RefSeq ORF | 1218 bp |
Locus ID | 25637 |
MW | 81.2 kDa |
Gene Summary | binds prostaglandin E2; plays a role in G-protein coupled receptor signaling and Ca2+ mobilization [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204660 | Ptger1 (untagged ORF) - Rat prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10 ug) |
CNY 5,488.00 |
|
RR204660L3 | Lenti ORF clone of Ptger1 (Myc-DDK-tagged ORF) - Rat prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10 ug) |
CNY 6,080.00 |
|
RR204660L4 | Lenti ORF clone of Ptger1 (mGFP-tagged ORF) - Rat prostaglandin E receptor 1 (subtype EP1) (Ptger1), (10 ug) |
CNY 6,650.00 |