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Prdm9 (NM_001108903) Rat Tagged ORF Clone
CAT＃: RR204955
- TrueORF®
Prdm9 (Myc-DDK-tagged ORF) - Rat PR domain containing 9 (Prdm9), (10 ug)
"NM_001108903" in other vectors (3)
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CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Prdm7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204955 representing NM_001108903
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGCACCATGAACACCAACAAGCCGGAAGAGAATAGTACTGAAGGAGATGCAGGGAAACTCGAGT GGAAACCCAAGGTCAAAGATGAGTTCAAAGACATTTCCATATACTTCTCCAAAGAAGAATGGGCAGAGAT GGGAGAGTGGGAGAAAATTCGCTATAGAAATGTGAAAAGGAACTATAAAATGCTGATTTCTATAGGTCTC AGAGCCCCTAGACCAGCTTTCATGTGTTACCAAAGGCAGGCAATCAAGCCCCAGATAAATGACAATGAGG ATTCTGATGAGGAATGGACACCAAAGCAGCAAGTCAGTTCTCCTTGGGTGCCTTTCCGGGTGAAGCACAG TAAACAACAGAAGGAAACACCCAGAATGCCATTAAGTGATAAATCTAGTGTGAAGGAAGTATTTGGAATA GAAAATTTGCTGAACACAAGTGGCTCAGAGCATGCCCAGAAACCAGTGTGCTCCCCTGAAGAAGGGAATA CCTCCGGACAGCACTTTGGAAAAAAACTGAAACTCAGGAGAAAGAATGTTGAAGTGAATAGGTACAGGCT GCGAGAGAGAAAGGACCTTGCATATGAAGAGGTCAGCGAGCCCCAGGATGATGACTACCTCTATTGTGAG AAGTGCCAGAATTTCTTCATCGACAGTTGTCCCAACCATGGGCCTCCTGTATTTGTAAAAGACAGTGTGG TGGACAGGGGGCATCCCAACCACTCAGTCCTCAGTCTGCCCCCTGGGCTGAGAATTGGTCCATCAGGCAT CCCTGAAGCTGGACTTGGAGTATGGAATGAAGCATCTGATCTACCAGTGGGTCTGCACTTTGGTCCCTAT AAGGGCCAGATTACAGAGGATGAAGAGGCAGCCAACAGTGGATACTCCTGGCTGATCACCAAGGGAAGAA ACTGCTATGAGTATGTGGATGGGCAGGATGAGTCCCAAGCCAACTGGATGAGGTATGTGAACTGTGCCCG GGATGACGAAGAGCAGAACCTGGTGGCCTTTCAATATCACAGGAAAATCTTCTATCGGACCTGCCGGGTC ATCAGGCCAGGTCGCGAGCTTCTGGTCTGGTATGGGGATGAGTATGGCCAAGAGCTGGGCATCAAGTGGG GAAGCAAGATGAAGAAAGGATTCACAGCAGGAAGAGAACTAAGGACAGAGATTCATCCTTGTTTTTTGTG CTCTTTGGCCTTCTCAAGTCAGAAATTCCTCACTCAACATGTAGAATGGAATCATCGCACAGAAATCTTC CCAGGAGCATCTGCAAGGATAAATCCTAAGCCAGGAGATCCCTGTCCAGATCAGCTTCAGGAACATTTTG ATTCACAGAACAAGAATGACAAAGCCAGCAATGAAGTCAAAAGAAAATCCAAACCCAGGCATAAATGGAC AAGACAGAGGATTTCAACAGCCTTTTCCAGTACTCTCAAAGAACAAATGAGATCTGAGGAAAGTAAGAGA ACTGTAGAAGAAGAGCTTAGAACAGGCCAGACAACAAATATAGAGGACACAGCCAAATCATTTATTGCAT CAGAAACCTCAAGAATTGAAAGGCAATGTGGGCAATGTTTCAGTGATAAGTCAAATGTCAGTGAGCACCA GAGGACACACACAGGGGAGAAGCCCTACATTTGCAGGGAGTGTGGGCGGGGCTTTTCACAGAAGTCAGAC CTCATCAAGCACCAGAGGACACACACAGAGGAGAAGCCCTACATTTGCAGGGAGTGTGGGCGGGGCTTTA CACAGAAGTCAGACCTCATCAAGCACCAGAGGACACACACAGAGGAGAAGCCCTACATTTGCAGGGAGTG TGGGCGGGGCTTTACACAGAAGTCAGACCTCATCAAGCACCAGAGGACACACACAGGGGAGAAGCCCTAC ATTTGCAGGGAGTGTGGGCGGGGCTTTACACAGAAGTCAGACCTCATCAAGCACCAGAGGACACACACAG AGGAGAAGCCCTACATTTGCAGGGAGTGTGGGCGGGGCTTTACACAGAAGTCAAGCCTCATCCGGCACCA GAGGACACACACAGGGGAGAAGCCCTACATTTGCAGGGAGTGTGGGCTGGGCTTTACACAGAAGTCAAAC CTCATCCGGCACCTGAGGACACACACAGGGGAGAAGCCCTACATTTGCAGGGAGTGTGGGCTGGGCTTTA CACGGAAGTCAAACCTCATCCAGCACCAGAGGACACACACAGGGGAGAAGCCCTACATTTGCAGGGAGTG TGGGCAGGGCCTTACATGGAAGTCAAGCCTCATCCAGCACCAGAGGACACACACAGGGGAGAAGCCCTAC ATTTGCAGGGAGTGTGGGCGGGGCTTTACATGGAAGTCAAGCCTCATCCAGCACCAGAGGACACACACAG TGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204955 representing NM_001108903
Red=Cloning site Green=Tags(s) MSRTMNTNKPEENSTEGDAGKLEWKPKVKDEFKDISIYFSKEEWAEMGEWEKIRYRNVKRNYKMLISIGL RAPRPAFMCYQRQAIKPQINDNEDSDEEWTPKQQVSSPWVPFRVKHSKQQKETPRMPLSDKSSVKEVFGI ENLLNTSGSEHAQKPVCSPEEGNTSGQHFGKKLKLRRKNVEVNRYRLRERKDLAYEEVSEPQDDDYLYCE KCQNFFIDSCPNHGPPVFVKDSVVDRGHPNHSVLSLPPGLRIGPSGIPEAGLGVWNEASDLPVGLHFGPY KGQITEDEEAANSGYSWLITKGRNCYEYVDGQDESQANWMRYVNCARDDEEQNLVAFQYHRKIFYRTCRV IRPGRELLVWYGDEYGQELGIKWGSKMKKGFTAGRELRTEIHPCFLCSLAFSSQKFLTQHVEWNHRTEIF PGASARINPKPGDPCPDQLQEHFDSQNKNDKASNEVKRKSKPRHKWTRQRISTAFSSTLKEQMRSEESKR TVEEELRTGQTTNIEDTAKSFIASETSRIERQCGQCFSDKSNVSEHQRTHTGEKPYICRECGRGFSQKSD LIKHQRTHTEEKPYICRECGRGFTQKSDLIKHQRTHTEEKPYICRECGRGFTQKSDLIKHQRTHTGEKPY ICRECGRGFTQKSDLIKHQRTHTEEKPYICRECGRGFTQKSSLIRHQRTHTGEKPYICRECGLGFTQKSN LIRHLRTHTGEKPYICRECGLGFTRKSNLIQHQRTHTGEKPYICRECGQGLTWKSSLIQHQRTHTGEKPY ICRECGRGFTWKSSLIQHQRTHTVEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108903 |
| ORF Size | 2388 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108903.2, NP_001102373.2 |
| RefSeq Size | 2688 bp |
| RefSeq ORF | 2391 bp |
| Locus ID | 365155 |
| UniProt ID | P0C6Y7 |
| MW | 92.6 kDa |
| Gene Summary | Histone methyltransferase that specifically trimethylates 'Lys-4' of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does not have the ability to mono- and dimethylate 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair
,null,
eLife
,PubMed ID 32744506
[Prdm9]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204955 | Prdm9 (untagged ORF) - Rat PR domain containing 9 (Prdm9), (10 ug) |
CNY 3,990.00 |
|
| RR204955L3 | Lenti ORF clone of Prdm9 (Myc-DDK-tagged ORF) - Rat PR domain containing 9 (Prdm9), (10 ug) |
CNY 6,080.00 |
|
| RR204955L4 | Lenti ORF clone of Prdm9 (mGFP-tagged ORF) - Rat PR domain containing 9 (Prdm9), (10 ug) |
CNY 6,650.00 |
