Als2 (NM_001013413) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205048 representing NM_001013413 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCAAAGAAGAAAAGCTCAGCAGAGGCAGAAGGATCCAAAGAAAGAGGCCTAGTCCATGTCTGGC AGGCAGGATCCTTTTCTCTAACACCAGAGAGGTTGCCAGGCTGGGGAGGAAAAACAGTTCTTCAGGCGGC CCTTGGCGTGAAGCATGGAGTTCTTCTGACTGAAGATGGTGAGGTCTACAGCTTTGGGACCCTTCCCTGG AAAAGTGAAGCAGCAGAAATTTGTCCAAGCAGCCCCCTTCTAGAAAGTGCCCTGGTTGGGCATCATGTTG TTACTGTGGCAACAGGGAGCTTCCACAGTGGAGCGGTGACAGAAAGTGGAGTGGTGTACATGTGGGGGGA GAACGCTGCCGGGCAGTGTGCAGTAGCGAACCAGCAGTATGTGTCGGAACCAAGTCCTGTCAGCATTTCT GACTCGGAGACCAGCCCCTTGCTAGCAGTTAGGATTCTGCAGTTGGCATGTGGCGAGGAACACACACTGG CATTGTCAATCAGCAGAGAGATCTGGGCATGGGGCACCGGCTGTCAGCTGGGTCTCATCACCACCACCTT CCCAGTGACAAAGCCACAAAAGGTGGAACACCTTGCTGGACGAGTGGTGCTCCAGGTGGCCTGTGGTGCG TTCCACAGCCTTGCACTTGTGCAGTGCCTCCCTCCCCAGGATCTGAAGCCAGTCCCAGAGAGATGCAATC AGTGCAGCCAGCTGCTCATCACCATGACAGACAAAGAGGACCACGTGATAATATCAGACAGCCATTGCTG CCCTTTAGGTGTGACACTGTCTGAATCTCAAGCAGAAAAGCACGCCAGCACTGTGACCAGCCCTCACCCG GAGACGCTGGATGGGCAAGGAGAGGTGTTTGAGAACACAGTAGCAGAAGCTGAACTGAATATGGGAAGCG ATCAGACCACAAGTGGCAGTGCCATTTCCGCCCAGCAGAACATCGTGGGAATGGCTGAAGTGTCTTCTGC CAGAACAGCTCCCTCATACCCAGACACCCAGACGGTAACTGCATACCTGCAGAAGCTATCAGAGCATTCA GTGAAGGAGAACCATGAGCGTGAGGAAAAGCTACCCCAGGTCCAGCCTCTTGTAGAAGAAGCAGTTCCTG ATCTTCACAGTCCACCAACCACAAGCACCTCAGCCCTCAACAGCTTGGTGGTCTCCTGTGCATCTGCTGT TGGTGTGAGAGTGGCCGCCACCTATGAAGCTGGGGCCTTGTCTCTCAAGAAAGTTATGAACTTTTATAGC ACTGCCCCCTGCGAGCCAGGAGCTCCGTCAGGCACTGCCTCCACAGGCCCAGAAAGTCTGAAAGATCTCA GAGAAGAGCAGGTGAAACAGGAATCACTACAAGGGAAGAAAAGCTCAAGCCTCATGGATATCAGAGAGGA GGAGTCGGAGGGAGGGAGTCGAAGACTCTCCCTCCCAGGATTGTTGTCGCAAGTTTCCCCCAGGCTCTTA AGGAAGGCCGCCAGGGTGAAAACACGGACAGTGGTTCTGACTCCCACGTACAGTGGAGAAGCAGATGCCC TCCTGCCTTCGCTGAGAACAGAGGTGTGGACCTGGGGGAAAGGCAAGGAAGGGCAGCTGGGGCACGGCGA TGTCCTGCCCAGGCTTCAGCCGTTGTGTGTCAAGTGTCTGGATGGTAAAGAGGTCATCCACTTGGAGGCA GGTGGCTCCCACTCTCTCGCACTGACTGCGAAATCTCAGGTTTACTCATGGGGCAGTAATACCTTCGGTC AGCTGGGGCATTCTGAGTTTCCAACAACGGTTCCTCGACTCTCGAAGGTTAGCAGTGAAAGTGGAGTCTG GAGTGTAGCTGCAGGCCACGGTTATTCCCTGTTTTTAGTGGACACTGAAGACTTCCAGCCTGGGTTGTAT TACAGTGGCCGCCAGGACCGTGCAGAAGGTGACACCCTGCCAGAGAATCCCAGTGGTACAACGACTCCGG TACTTCTCTCCTGTAGTAAGCTTGGATATATCAGCAGAGTGACAGCAGGAAAAGATAGCTACCTAGCCTT GGTGGATAAGAACATCATGGGATACATCGCCAGCCTCCATGAGTTGGCTACCACAGAAAGACGGTTTTAC TCGAAACTAAGTGAAATCAAGTCACAGATACTTAGGCCTCTTCTCAGTCTGGAACATTTGGGCACTGTGA CCACTGTCCAGCTGTTGCAGGAAGTCGCCAGCCGGTTCAGCAAGTTGTGTTACCTCATTGGGCAGCATGG AGCCTCACTGAGCAGCTACCTGCAAGGCATGAAGGAAGCCAGGAACCTGGTCATCATGAAGCACTCGAGT CTTTTCTTGGACAGTTACACAGAGTATTGTACATCAGTTTCAAATTTTCTGGTTATGGGAGGATCCCAGC TTCTTGCTAAGCCTGCAATTGATTTCCTAAATAAAAACCAAGAACTCTTGCAAGATTTGTCAGAAGTGAA TGATGAGAACACTCAGTTGATGGAAATACTGAATGCGCTGTTTTTCTTGCCAATCAGACGACTTCATAAT TATGCAAAAGTTTTGCTAAAGCTCGCCACTTGCTTTGAAGTGACATCACCAGAGTATCAAAAGCTGCAGG ATTCCAGTTCTTGTTATGAGTCTCTTGCTCTCCATCTTGGCAAGAAGAGGAAGGAAGCAGAGTACACGCT GAGCTTCTGGAAGACCTTCCCTGGAAAAATGACGGATTCCTTGAGGAAGCCAGAGCGCCGGCTGCTGTGT GAGAGCAGTAACCGAGCCCTCTCCCTGCAGCATGCTGGCAGGTTTTCTGTGAATTGGTTCATTCTCTTCA ATGATGCCCTGGTCCATGCTCAGTTCTCCACACACCACGTGTTCCCTTTGGCCACACTCTGGGCAGAGCC ACTTTCTGAAGAAACTGGTGGCGTGAATGGCTTAAAGATAACCACACCTGAAGAACAGTTCACGCTCATT TCTTCAACACCCCAGGAAAAGACCAAGTGGCTTCGGGCTATCAGCCAAGCCGTGGACCAGGCTTTGAGGG GGACGTCTGACTTCCCACTTTACGGAGGCAGCAGCACTGTTCAGAGGCAGGAACCACCCATCTCAAGAAG TGCCAAATACACTTTCTACAAGGATACTCGCCTAAAGGACGCCACTTACGATGGGCGCTGGCTTTCAGGG AAGCCGCACGGAAGGGGTGTTTTGAAGTGGCCTGATGGGAAGGTGTATTCTGGCACGTTCAGGAATGGCT TGGAAGACGGATATGGTGAATACAGAATCCCAAACAAGGCCCTGAACAAAGAAGACCATTACGTGGGCCA TTGGAAGGAGGGGAAAATGTGTGGGCAAGGAGTGTACAGCTATGCCTCTGGTGAAGTGTTTGAAGGCTGC TTTCAAGATAACATGCGCCACGGGCACGGTCTCCTCCGCAGTGGAAAACTGACTTCTTCCTCTCCCAGCA TGTTCATTGGCCAGTGGGTAATGGACAAGAAAGCAGGGTATGGCGTCTTCGATGATATCACCAGGGGAGA AAAGTACATGGGAATGTGGCAGGACGATGCATGCCAAGGGAATGGGGTGGTGGTCACCCAGTTTGGGTTA TACTACGAAGGCAACTTCCACCTTAATAAAATGATGGGAAATGGGGTTTTGCTTTCGGAAGATGATACTA TATATGAAGGAGAATTTTCTGATGACTGGACACTTTGTGGAAAGGGAACACTGACTATGCCAAATGGAGA TTATATTGAAGGTTATTTTAGTGGAGAATGGGGATCTGGGATAAAAATCACTGGGACCTACTTCAAACCT AGCCTATATGAGAGTGATAAGGACAGGCCCAAAGCCTTCAGGAAGCTGGGAAACCTGGCCGTGGCCGCCG ATGAGAAATGGAGAGCAGTGTTTGACGAATGCTGGCGCCAGCTGGGCTGTGAGAGCCCAGGCCAAGGGGA GGTTTGGAAAGCGTGGGATAATATTGCTGTGGCCTTGACCACCAACCGTCGCCAGCATAAAGACAGTCCA GAAATACTAAGCCGTTCACAGACTCAGACCCTGGAGAGTTTGGAGTACATTCCCCAGCACGTTGGCGCCT TCTCTGTGGAGAAATATGATGATATCAAGAAGTATTTAATAAAGGCCTGTGACACTCCTCTGCACCCACT GGGCAGGCTTGTGGAGACCCTGGTTGCAGTGTATAGAATGACTTATGTGGGCGTAGGGGCCAACCGTCGG TTACTGCAGGAAGCTGTGAAGGAGATTAAGTCTTATCTCAAGAGGATTTTCCAACTTGTGAGGTTCTTGT TTCCTGAGCTTCCTGAAGAGGGCAGCACAGTTCCTCTCTCTGCTCCTCTGCCCACTGGAAGAAGATCCTT CTGCACTGGGAAATCAGATTCCAGATCCGAGTCACCAGAGCCAGGTTATGTAGTAACAAGTTCTGGCTTA CTGCTTCCGGTGCTGCTGCCTCGGCTCTACCCGCCTCTCTTCATGCTCTATGCCCTGGACAATGACCGAG AGGAAGACATTTACTGGGAATGTGTGCTTCGACTAAACAAGCAGCCAGATATTGCTCTCCTGGGCTTTCT TGGAGTACAGAGGAAATTCTGGCCAGCAACCTTGTCAATCCTTGGAGAGAGTAAAAAGGTACTGCCATCC ACAAAGGATGCTTGTTTTGCATCTGCAGTAGAATGTCTGCAGCAGATCAGCACAACATTTACTCCATCAG ACAAACTTAAAGTGATCCAGCAGACCTTCGAAGAAATCTCTCAGAGTGTGCTGGCGTCGCTGCAGGAGGA CTTCCTTTGGTCCATGGATGACTTGTTCCCTGTCTTCTTATACGTGGTGCTGCGGGCCAGGATTCGGAAT TTAGGCTCTGAAGTTCATCTCATTGAGGATCTGATGGACCCCTATCTCCAGCATGGGGAGCAAGGCATAA TGTTCACCACCTTGAAGGCCTGTTACTACCAGATTCAGCGTGAGAAGCTTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205048 representing NM_001013413 Red=Cloning site Green=Tags(s) MDSKKKSSAEAEGSKERGLVHVWQAGSFSLTPERLPGWGGKTVLQAALGVKHGVLLTEDGEVYSFGTLPW KSEAAEICPSSPLLESALVGHHVVTVATGSFHSGAVTESGVVYMWGENAAGQCAVANQQYVSEPSPVSIS DSETSPLLAVRILQLACGEEHTLALSISREIWAWGTGCQLGLITTTFPVTKPQKVEHLAGRVVLQVACGA FHSLALVQCLPPQDLKPVPERCNQCSQLLITMTDKEDHVIISDSHCCPLGVTLSESQAEKHASTVTSPHP ETLDGQGEVFENTVAEAELNMGSDQTTSGSAISAQQNIVGMAEVSSARTAPSYPDTQTVTAYLQKLSEHS VKENHEREEKLPQVQPLVEEAVPDLHSPPTTSTSALNSLVVSCASAVGVRVAATYEAGALSLKKVMNFYS TAPCEPGAPSGTASTGPESLKDLREEQVKQESLQGKKSSSLMDIREEESEGGSRRLSLPGLLSQVSPRLL RKAARVKTRTVVLTPTYSGEADALLPSLRTEVWTWGKGKEGQLGHGDVLPRLQPLCVKCLDGKEVIHLEA GGSHSLALTAKSQVYSWGSNTFGQLGHSEFPTTVPRLSKVSSESGVWSVAAGHGYSLFLVDTEDFQPGLY YSGRQDRAEGDTLPENPSGTTTPVLLSCSKLGYISRVTAGKDSYLALVDKNIMGYIASLHELATTERRFY SKLSEIKSQILRPLLSLEHLGTVTTVQLLQEVASRFSKLCYLIGQHGASLSSYLQGMKEARNLVIMKHSS LFLDSYTEYCTSVSNFLVMGGSQLLAKPAIDFLNKNQELLQDLSEVNDENTQLMEILNALFFLPIRRLHN YAKVLLKLATCFEVTSPEYQKLQDSSSCYESLALHLGKKRKEAEYTLSFWKTFPGKMTDSLRKPERRLLC ESSNRALSLQHAGRFSVNWFILFNDALVHAQFSTHHVFPLATLWAEPLSEETGGVNGLKITTPEEQFTLI SSTPQEKTKWLRAISQAVDQALRGTSDFPLYGGSSTVQRQEPPISRSAKYTFYKDTRLKDATYDGRWLSG KPHGRGVLKWPDGKVYSGTFRNGLEDGYGEYRIPNKALNKEDHYVGHWKEGKMCGQGVYSYASGEVFEGC FQDNMRHGHGLLRSGKLTSSSPSMFIGQWVMDKKAGYGVFDDITRGEKYMGMWQDDACQGNGVVVTQFGL YYEGNFHLNKMMGNGVLLSEDDTIYEGEFSDDWTLCGKGTLTMPNGDYIEGYFSGEWGSGIKITGTYFKP SLYESDKDRPKAFRKLGNLAVAADEKWRAVFDECWRQLGCESPGQGEVWKAWDNIAVALTTNRRQHKDSP EILSRSQTQTLESLEYIPQHVGAFSVEKYDDIKKYLIKACDTPLHPLGRLVETLVAVYRMTYVGVGANRR LLQEAVKEIKSYLKRIFQLVRFLFPELPEEGSTVPLSAPLPTGRRSFCTGKSDSRSESPEPGYVVTSSGL LLPVLLPRLYPPLFMLYALDNDREEDIYWECVLRLNKQPDIALLGFLGVQRKFWPATLSILGESKKVLPS TKDACFASAVECLQQISTTFTPSDKLKVIQQTFEEISQSVLASLQEDFLWSMDDLFPVFLYVVLRARIRN LGSEVHLIEDLMDPYLQHGEQGIMFTTLKACYYQIQREKLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001013413
ORF Size	4953 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001013413.1, NP_001013431.1
RefSeq Size	5068 bp
RefSeq ORF	4956 bp
Locus ID	363235
UniProt ID	P0C5Y8
MW	182.4 kDa
Gene Summary	May act as a GTPase regulator (By similarity). Controls survival and growth of spinal motoneurons.[UniProtKB/Swiss-Prot Function]

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