Robo1 (NM_022188) Rat Tagged ORF Clone
CAT#: RR205390
- TrueORF®
Robo1 (Myc-DDK-tagged ORF) - Rat roundabout homolog 1 (Drosophila) (Robo1), (10 ug)
"NM_022188" in other vectors (1)
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CNY 16,944.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR205390 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATGGAAACATCTTCCTCTTCTGGTCATGATATCTCTCCTCACTCTATCAAAAAAGCATCTCCTTC TGGCCCAGCTTATTCCAGACCCTGAGGACCTAGAGAGGGGGAACGACAACGGGACGCCAGCCCCCACCTC TGATAATGATGACAATTCGCTGGGCTACACAGGCTCCCGTCTCCGTCAGGAAGATTTCCCACCGCGCATT GTTGAGCACCCTTCAGACCTGATCGTCTCTAAAGGGGAACCCGCCACCCTCAACTGTAAAGCTGAAGGCC GCCCTACCCCGACTATAGAATGGTACAAGGGCGGCGAAAGAGTGGAAACGGACAAAGACGACCCTCGCTC ACACCGAATGCTGCTGCCAAGTGGATCTTTATTTTTCTTACGCATAGTGCATGGACGGAAGAGTAGGCCT GACGAAGGGGTCTACATCTGTGTAGCAAGGAATTACCTCGGAGAGGCTGTGAGCCACAATGCATCGCTGG AGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTTATGGTGGCGGTGGGCGAGCCTGC AGTAATGGAATGCCAGCCTCCTCGGGGCCACCCAGAGCCCACCATCTCCTGGAAAAAGGATGGCTCACCC CTGGATGATAAAGACGAGCGAATCACTATCCGAGGCGGGAAGCTCATGATCACGTACACCCGTAAAAGCG ATGCTGGCAAGTATGTTTGTGTTGGTACCAACATGGTAGGGGAGCGGGAGAGCAAAGTCGCGGACGTGAC TGTCTTAGAGAGACCGTCCTTTGTAAAGAGACCCAGTAATTTGGCAGTGACTGTGGATGACAGTGCTGAG TTTAAGTGTGAGGCCCGAGGAGACCCCGTGCCTACGTTCGGGTGGAGGAAAGATGACGGAGAGCTGCCCA AATCCAGATATGAGATCCGAGATGATCATACTTTGAAAATTCGGAAGGTGACAGCTGGCGACATGGGATC ATACACTTGTGTTGCAGAAAATATGGTTGGGAAAGCCGAAGCATCAGCCACTTTGACAGTTCAAGAGCCA CCACATTTTGTCGTGAAGCCTCGAGACCAGGTGGTTGCCTTGGGGCGAACTGTGACTTTTCAGTGTGAAG CAACTGGAAATCCTCAACCAGCTATCTTTTGGCGGAGAGAGGGGAGTCAGAATCTGCTTTTCTCATACCA GCCTCCACAGTCCTCTAGCCGGTTTTCAGTTTCCCAGACGGGGGACCTCACTGTTACGAACGTCCAGAGG TCTGATGTCGGTTACTATATCTGTCAGACTTTGAACGTGGCTGGAAGCATCATCACAAAGGCATATTTGG AAGTTACTGATGTGATTGCGGACCGGCCTCCCCCAGTCATCCGACAAGGTCCTGTGAATCAGACTGTAGC AGTGGATGGTACTTTAACTCTCAGCTGTGTGGCCACAGGCAGTCCCGTGCCCACAATTCTGTGGAGGAAG GATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGAGAGCGGGGTCCTGCAGATCCGCT ACGCTAAGCTGGGTGACACCGGTCGGTACACCTGCACTGCTTCGACCCCCAGTGGTGAGGCCACGTGGAG CGCTTACATCGAAGTCCAGGAATTTGGAGTTCCAGTTCAACCTCCAAGACCCACCGACCCCAATTTAATC CCCAGTGCGCCTTCAAAGCCTGAAGTCACAGATGTGAGCAAAAACACAGTCACTTTATTGTGGCAGCCTA ACCTGAACTCAGGAGCAACCCCAACGTCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAGCTG GCAGACCGTGGCAGAAAATGTGAAAACAGAAACATTCGCCATCAAAGGGCTCAAACCTAATGCGATCTAC CTTTTCCTGGTGAGGGCAGCAAATGCCTATGGGATTAGCGATCCAAGCCAAATATCCGATCCAGTGAAAA CACAAGATGTCCCACCAACCACCCAAGGGGTGGACCACAAGCAGGTCCAGAGAGAACTGGGCAATGTTGT CTTGCATCTCCACAACCCTACCATCCTTTCTTCCTCTTCTGTCGAAGTGCACTGGACGGTGGACCAACAA TCTCAATATATTCAAGGTTATAAAATTCTCTACCGACCATCTGGAGCCAGCCATGGCGAATCGGAGTGGT TAGTTTTTGAAGTGAGGACCCCAACCAAAAATAGTGTGGTGATCCCCGATCTGAGAAAAGGCGTCAACTA CGAGATTAAGGCCCGCCCATTTTTTAATGAGTTCCAAGGAGCAGATAGTGAAATCAAATTTGCCAAAACC TTAGAAGAACGACCAAGTGCCCCTCCCCGAAGTGTCACTGTATCCAAGAATGATGGAAATGGGACAGCAA TTCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAGAATGGAATGGTCCAAGAATATAAGGTTTGGTG TCTCGGGAATGAGACTAGGTATCACATAAACAAGACAGTAGATGGCTCTACCTTTTCGGTGGTCATTCCC TTTCTTGTTCCTGGAATCCGCTACAGTGTAGAAGTGGCTGCAAGCACTGGGGCTGGCCCTGGGGTGAAGA GTGAGCCCCAGTTCATCCAGTTGGATTCCCATGGAAACCCCGTGTCTCCCGAGGACCAAGTGAGCCTGGC TCAGCAGATCTCCGACGTGGTGAAGCAGCCAGCCTTCATTGCGGGCATCGGGGCCGCCTGTTGGATCATT CTCATGGTCTTCAGTATCTGGCTTTACCGACACCGCAAGAAGAGAAACGGGCTCAGCAGCACGTACGCGG GGATCCGGAAAGTCCCGTCCTTTACCTTCACACCAACAGTAACTTATCAAAGAGGAGGCGAAGCCGTCAG CAGTGGAGGGAGGCCGGGACTCCTCAACATCAGTGAACCTGCCACACAGCCATGGCTGGCGGACACGTGG CCCAACACAGGCAACAGTCACAATGACTGCTCCATCAACTGCTGCACAGCCAGCAACGGCAACAGCGACA GCAACCTGACCACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAAACA AACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAAAATCAATGAGATG AAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCCATCAGGGCAGCCCACTCCCTACG CCACCACGCAGCTCATCCAGGCAAATCTCATCAACAATATGAATAACGGCGGCGGGGACTCCAGCGAGAA ACACTGGAAGCCTCCGGGGCAGCAGAAACAGGAAGTGGCACCAATTCAGTATAACATCATGGAGCAAAAC AAGTTGAACAAAGATTATCGGGCAAATGACACAATCCTTCCAACCATCCCGTACAACCATTCNTATGACC AGAATACAGGAGGATCCTACAACAGCTCAGATCGGGGCAGTAGTACATCTGGGAGTCAAGGCCACAAGAA AGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGGCAGACTTGCTGCCCCCTCCCCCA GCGCATCCTCCCCCACACAGCAACAGCGAGGAATACAGCATGTCTGTAGATGAAAGCTATGATCAAGAAA TGCCGTGCCCTGTTCCGCCTGCACGGATGTATTTGCAACAGGATGAATTAGAAGAAGAGGAAGCTGAAAG AGGCCCCACGCCCCCTGTCCGGGGGGCAGCTTCGTCCCCAGCTGCTGTGTCCTACAGCCATCAGTCCACG GCCACTCTCACCCCGTCTCCCCAGGAAGAGCTCCAGCCTATGTTACAGGATTGTCCGGAGGACCTCGGCC ACATGCCCCACCCACCAGACAGGAGGCGGCAGCCGGTGAGTCCTCCTCCACCACCACGGCCAATTTCCCC ACCACATACTTACGGCTACATTTCAGGACCCCTGGTCTCAGACATGGATACGGATGCCCCAGAAGAGGAG GAAGACGAAGCTGACATGGAAGTTGCTAAGATGCAGACCCGAAGGCTGTTGTTACGTGGGCTTGAACAGA CCCCAGCATCTAGTGTTGGGGACCTCGAGAGCTCGGTCACTGGCTCCATGATCAATGGCTGGGGCTCAGC CTCAGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCATCGGATGGTTCCTTTTTCACTGAC GCGGATTTTGCCCAGGCAGTTGCAGCAGCCGCCGAGTATGCGGGCCTGAAGGTGGCTCGCCGCCAGATGC AGGATGCTGCTGGCCGCCGACACTTCCATGCCTCCCAGTGCCCTAGGCCCACGAGTCCTGTGTCTACAGA CAGCAACATGAGTGCTGCTGTAATCCAGAAAGCCAGACCCACCAAGAAACAGAAGCACCAGCCAGGACAT CTGCGCAGAGAGGCCTACACAGATGATCTTCCACCACCTCCAGTGCCACCGCCTGCTATAAAATCGCCCA GTGTCCAGTCCAAGGCACAGCTGGAGGCACGGCCTATAATGGGGCCAAAGCTCGCTTCTATAGAAGCGAG AGCAGACAGATCATCAGACAGAAAAGGCGGCAGTTACAAGGGGAGAGAAGCTCTGGACGGAAGACAAGTC ACTGACCTGCGAACAAGCCCAGGTGACCCCAGAGAAGCACAGGAGCAGCCAAACGAAGGGAAAGCACGAG GGACGAAGACAGCAAAGCGAGACCTTCCACCAGCGAAGACACACCTCATCCCAGAGGATATCCTTCCGTA CTGTAGGCCTACTTTCCCAACGTCAAACAACCCTAGGGATCCCAGTTCTTCAAGCTCAATGTCATCAAGA GGCTCAGGAAGCCGACAGAGAGAGCAAGCAAATGTGGGCCGAAGAAATATGGCAGAAATGCAAGTACTTG GGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACTGAAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205390 protein sequence
Red=Cloning site Green=Tags(s) MKWKHLPLLVMISLLTLSKKHLLLAQLIPDPEDLERGNDNGTPAPTSDNDDNSLGYTGSRLRQEDFPPRI VEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRP DEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSP LDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAE FKCEARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEP PHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQR SDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRK DGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLI PSAPSKPEVTDVSKNTVTLLWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIY LFLVRAANAYGISDPSQISDPVKTQDVPPTTQGVDHKQVQRELGNVVLHLHNPTILSSSSVEVHWTVDQQ SQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKT LEERPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIP FLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWII LMVFSIWLYRHRKKRNGLSSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPATQPWLADTW PNTGNSHNDCSINCCTASNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEM KTFNSPNLKDGRFVNPSGQPTPYATTQLIQANLINNMNNGGGDSSEKHWKPPGQQKQEVAPIQYNIMEQN KLNKDYRANDTILPTIPYNHXYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKAPKQGGMNWADLLPPPP AHPPPHSNSEEYSMSVDESYDQEMPCPVPPARMYLQQDELEEEEAERGPTPPVRGAASSPAAVSYSHQST ATLTPSPQEELQPMLQDCPEDLGHMPHPPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEE EDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTD ADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVIQKARPTKKQKHQPGH LRREAYTDDLPPPPVPPPAIKSPSVQSKAQLEARPIMGPKLASIEARADRSSDRKGGSYKGREALDGRQV TDLRTSPGDPREAQEQPNEGKARGTKTAKRDLPPAKTHLIPEDILPYCRPTFPTSNNPRDPSSSSSMSSR GSGSRQREQANVGRRNMAEMQVLGGFERGDENNEELEETES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022188 |
ORF Size | 4953 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022188.1, NP_071524.1 |
RefSeq Size | 4956 bp |
RefSeq ORF | 4956 bp |
Locus ID | 58946 |
UniProt ID | O55005 |
MW | 180.8 kDa |
Gene Summary | putative axon guidance receptor [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN205390 | Robo1 (untagged ORF) - Rat roundabout homolog 1 (Drosophila) (Robo1), (10 ug) |
CNY 26,510.00 |