Nsd3 (NM_001106090) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Whsc1l1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR205449 representing NM_001106090 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTAGGATCATGGGAAACACAATTCAGCAACCACCTCAACTCATTGACTCGGCCAATATCCGCC AGGAGGATGCCTTTGATAACAACAGTGACATTGTTGAAGATGGTGGCCCGACACCCTTCGAAGCTACTTT GCAACAAGGTTTCCAATGTCCACCTGCAACAGAAGACCTTCCTCCACTCACAAACGGCTACCCACCGTCC ATCAGCTTGTATGAGACTCAAACCAAATACCCGCCATATAGCCAGTATCCCAATGGGTCAGCCAACGGTT TTGGTGCAGTTAGAAACTTTAGTCCTACTGACTATTACCATTCAGAAATTCCAAACACAAGACCACATGA AATTCTGGAAAAACCGTCTCCCCCACAGCCTCCTCCTCCTCCTCTTCTTCCTCCTCCTCCTCCTCCGGTA CCACAAACTGTGATTCCAAAGAAGACAGGCTCACCCGAGATTAAACTAAAAATAACCAAAACTATCCAGA ATGGCAGGGAATTGTTTGAGTCCTCCCTCTGTGGAGACCTTTTAAACGAAGTACAGGCCAGTGAGCACTT AAAGTCAAAGCACGAAAGCAGAAAGGAAAAGAGGAAAAAAAGCAGCAGACACGAGTCATCGAGATCCGAA GAGCGAAAGTCACACAAGGTCCCCAAGTTAGAGCCAGAGGGGCAGAATAGACCAAATGAGAGGGTGGACC CTGCACCAGAAAAGCCAAGAGAAGAGCCAGTGCTCAAAGAGGCCGTCCCGGTTCAGCCAATACTGTCTTC TGTTCCAACAACAGAAACGTCCACTGGTGTTAAGTTCCAGGTTGGTGATCTTGTTTGGTCCAAGGTGGGA ACCTATCCTTGGTGGCCTTGTATGGTTTCAAGTGATCCCCAGCTTGAGGTCCATACCAAAATTAACACAA GAGGTGCCCGGGAGTATCATGTCCAGTTTTTTAGCAACCAGCCAGAGAGGGCATGGGTTCATGAGAAGCG GGTACGGGAGTATAAAGGTCACGAACAGTATGAAGAGTTACTAGCAGAGGCAGCCAAACAAGCCAGCAAC CATTCTGAAAAGCAAAAGATTCGGAAACCCCGACCTCAGAGAGAACGCGCCCAGTGGGATATTGGCATTG CTCACGCAGAGAAAGCATTGCAGATGACACGAGAGGAAAGGGTAGAACAGTACACGTTCATCTATATCGA TAAGCAGCCAGAAGAGTCTCCGTCCCAAGCAAAGAAGAGTGTTACCTCTAAGGCGGAAGTCAAGAAACCC CGAAGGCCCAGATCTGTGGGGAACAGTCAGCCAGAACAGACCAGTGCTGGGGAGGTGGCTTCCCCACAGT CGAGTACTGACCCTCGCAGGCAGAGCCAGAGGCGGCACACCAGTGTGGAAGAGGACGGGCCGCCTCCCGT TAAAATAGCCTGGAGAACAGCCGCCGCCAGGAAGTCCTTACCAGCCTCCATCACAATGCACAAGGGGAGC CTAGATCTGCACAAGTGTAATATGTCTCCAGTTGTGAAAATTGAACAAGTGTTTGCTCTCCAGAATGCAA CAGGAGATGGGAAGTTTATTGATCAGTTTGTTTATTCAACGAAGGGAATTGGTAACAAAACAGAAATAAG TGTCAGGGGGCAAGACAGGCTTATAATCTCTTCACCAAGTCAGAGAAACGAGAAGTCGACTCCGAGCGCG TCACCTCCGGAGGCCACATCTGGTTCTGCAGGCCCAGTAGAAAAGAAGCAGCAGAGAAGATCAATCAGGA CTCGATCTGAGTCAGAGAAATCCACCGAGGTTGTGTCAAAGAAGAAGATCAAAAAGGAGCAGGTTGAAAC ATCTCCTCAGGCTTCCCTGAAGACCGGATTACAGAAAGGTGCCAGCGAGATTTCAGATTCCTGTAAACCT CTAAAGAAAAGGAGTCGCGCCTCGACTGATGTGGAAACGGCCAGTTGCACGTACAGAGACACATCTGACT CCGATTCCAGAGGACCGAGTGATGGGCAGGTAGGCTTTGGAAAACAAGTAGATAGCCCGTCAGCAGCTGC TGATGCAGATGCTTCTGATGCACAGTCGGTGGACTCGAGCCTGTCACGGAGAGGCGTCGGCCTGAGTAAG AAGGACACTGTGTGTCAGGTCTGTGAGAACACTGGAGACTCTCTCGTTGCTTGTGAGGGGGAATGCTGCA GACACTTTCATCCGGAGTGCTTGGGGCTGGCTGCGGTCCCTGAGGGACGCTTCACCTGTGAGGAGTGTGA GACAGGGCAGCACCCATGTTTTTCATGTAAAGTGTCTGGTAAGGATGTGAAACGTTGTTCTGTTAGTGTT TGTGGAAAGTTTTATCATGAAGCCTGTGTCCGCAAATTCCCCACTGCCATCTTTGAGTCAAAAGGATTCC GCTGTCCCCAGCACTGTTGTTCTTCCTGCTCCATGGAGAAGGACATCCACAAAGCGAGCAAAGGCCGGAT GATGAAGTGCTTGAGATGCCCAATCGCCTACCACTTTGGAGATGCGTGTGTCGCTGCTGGAAGTGTATCT GTGTCCTCCCACATTCTCATCTGTAGTAACCATTCCAAGCGGAGTAGTCATTCTGCTGCTATAAACGTAG GCTTTTGTTTCGTTTGTGCCAGAGGTGGAAGATTGCTCTGCTGTGAATCGTGCCCTGCTTCCTTCCACCC AGAATGCCTGAGCATAGACATGCCCGAAGGCTGCTGGAATTGCAATGACTGTAAAGCTGGCAAGAAGCTG CATTACAAACAGATTGTTTGGGTCAAATTGGGAAATTACAGGCAAGTTTCCCAAGCAGATAGGAAATGGT GGCCTGCAGAGACCTGCAGTCCCAGGTCTGTGCCGCTGAACATCCAGGGCCTTAAGCACGGCGTGGGCGA CTTCCCTGTGCTCTTCTTTGGCTCTCATGACTACTACTGGGTCCACCAAGGCAGAGTGTTTCCCTATGTT GAAGGAGACACAAACTTTGCTGAGGGACAGACTAGCATTAACAAGACCTTCAAAAAAGCACTGGAAGAAG CTGCAAAACGTTTCCAGGAGTTGAAAGCGCAGCGAGAGAGTAAGGAGGCTTTGGAGATAGAGAAAACCTC AAGAAAACCCCCTCCCTACAAACACATCAAGGCCAACAAAGTCATAGGGAAGGTGCAGATCCAGGTGGCT GACCTGTCGGAGATCCCGCGCTGTAACTGCAAGCCTGGTGATGAAAACCCATGTGGCCTGGAGTCGGAGT GTCTAAACAGAATGTCACAGTACGAGTGTCACCCGCAGGTGTGCCCTGCTGGGGACCGCTGTCAGAACCA GTGCTTTACAAAGAGGCTGTACCCCGATGCAGAGATCATCAAAACTGAGCGGAGAGGATGGGGCCTCAGA ACCAAAAGAAGTATTAAAAAGGGGGAATTTGTAAACGAATATGTCGGTGAACTAATCGACGAGGAAGAGT GCAGACTTCGAATCAAGCGAGCCCACGAGAACAGTGTAACTAATTTTTATATGTTAACTGTTACCAAGGA CCGTATAATTGATGCTGGACCAAAGGGAAATTATTCTCGTTTCATGAACCACAGTTGTAATCCAAACTGT GAGACACAGAAGTGGACCGTGAATGGAGATGTCCGAGTCGGGCTGTTCGCTCTTTGTGACATCCCTGCAG GGATGGAGTTAACATTTAATTACAACTTGGACTGTCTGGGCAATGGCAGGACAGTATGCCACTGCGGAGC AGACAACTGCAGTGGTTTCCTAGGAGTGCGGCCAAAGTCTGCATGTACGTCAGCAGCTGACGAGAAGACA AAAAATGCCAAGTTAAAGAAGAGGCGGAGAGGCAAAGCAGAGGCCAAGCAAATCCATGAGGATTATTGTT TTCAGTGTGGGGATGGTGGAGAGTTGGTCATGTGTGACAAAAAAGACTGTCCCAAAGCGTACCACCTCCT ATGCCTTAACCTGACTCAGCCACCCTACGGAAAGTGGGAGTGCCCGTGGCATCAGTGTGACGAGTGTGGC AGTGCAGCCATTTCCTTCTGTGAATTCTGTCCACACTCCTTCTGTAAGGCTCATGAAAAGGGGGCTCTGG TACCCTCTGCACTAGAAGGTCGTCTCTGCTGCTCCAACCATGACCCTGCATCTCCTGTGTCACCGGAATA CTGGAGCAAGATGAGATGTAAATGGGAATCCCAAGATGGTGGGGAAGAAGTAAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR205449 representing NM_001106090 Red=Cloning site Green=Tags(s) MASRIMGNTIQQPPQLIDSANIRQEDAFDNNSDIVEDGGPTPFEATLQQGFQCPPATEDLPPLTNGYPPS ISLYETQTKYPPYSQYPNGSANGFGAVRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPLLPPPPPPV PQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHLKSKHESRKEKRKKSSRHESSRSE ERKSHKVPKLEPEGQNRPNERVDPAPEKPREEPVLKEAVPVQPILSSVPTTETSTGVKFQVGDLVWSKVG TYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHEQYEELLAEAAKQASN HSEKQKIRKPRPQRERAQWDIGIAHAEKALQMTREERVEQYTFIYIDKQPEESPSQAKKSVTSKAEVKKP RRPRSVGNSQPEQTSAGEVASPQSSTDPRRQSQRRHTSVEEDGPPPVKIAWRTAAARKSLPASITMHKGS LDLHKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISSPSQRNEKSTPSA SPPEATSGSAGPVEKKQQRRSIRTRSESEKSTEVVSKKKIKKEQVETSPQASLKTGLQKGASEISDSCKP LKKRSRASTDVETASCTYRDTSDSDSRGPSDGQVGFGKQVDSPSAAADADASDAQSVDSSLSRRGVGLSK KDTVCQVCENTGDSLVACEGECCRHFHPECLGLAAVPEGRFTCEECETGQHPCFSCKVSGKDVKRCSVSV CGKFYHEACVRKFPTAIFESKGFRCPQHCCSSCSMEKDIHKASKGRMMKCLRCPIAYHFGDACVAAGSVS VSSHILICSNHSKRSSHSAAINVGFCFVCARGGRLLCCESCPASFHPECLSIDMPEGCWNCNDCKAGKKL HYKQIVWVKLGNYRQVSQADRKWWPAETCSPRSVPLNIQGLKHGVGDFPVLFFGSHDYYWVHQGRVFPYV EGDTNFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKTSRKPPPYKHIKANKVIGKVQIQVA DLSEIPRCNCKPGDENPCGLESECLNRMSQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLR TKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNC ETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTVCHCGADNCSGFLGVRPKSACTSAADEKT KNAKLKKRRRGKAEAKQIHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECG SAAISFCEFCPHSFCKAHEKGALVPSALEGRLCCSNHDPASPVSPEYWSKMRCKWESQDGGEEVKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001106090
ORF Size	4188 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001106090.1, NP_001099560.1
RefSeq Size	4863 bp
RefSeq ORF	4191 bp
Locus ID	290831
MW	155.6 kDa

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