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Opa1 (NM_133585) Rat Tagged ORF Clone
CAT＃: RR205659
- TrueORF®
Opa1 (Myc-DDK-tagged ORF) - Rat optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_133585" in other vectors (3)
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CNY 12,350.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MGC124921 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205659 representing NM_133585
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGAGCAGGTCGGGCTGCCCTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAGCACAGCTCTGGAG TACAAAGAAACGTACCGCTCCAAAAACTGCATCTGGTTTCCCGAAGTATTTATCGTTCACATCATCCTGC CCTCAAGCTTCAAAGACCCCAATTAAGGACATCTTTTCAGCAATTCTCTTCTCTAACTAACCTTTCTTTA CATAAATTGAAACTTTCTCCAACTAAATATGGCTACCAACCCCGCAGGAACTTTTGGCCAGCAAGGTTAG CTGCAAGGCTCTTAAAACTTCGATATATTATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA TGGGAAATTGATGAGTATATTGATTTGGAGAAAATCAGAAAAGCCCTTCCCAGTTCAGAAGACCTCGCCA ATTTCGCTCCTGACCTGGACAAGATCGCCGAAAGCCTCAGCTTGTTGAAGGATTTCTTCACTGCGGGTAC ACCTGGAGAAACAGCATTTAGAGCAACAGATCATGGATCCGAAAGTGACAAGCACTATAGGAAGGTGTCA GACAAAGAAAAGATTGACCAACTTCAGGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCCTGG AGCGTTTGGAGAAGGAGAACAAAGAGTTGAGGAAGTTGGTGCTGCAGAAGGATGACAAAGGCATCCACCA CAGGAAGCTCAAGAAATCTCTGATCGATATGTATTCTGAAGTTCTTGATGTCCTTTCTGACTATGATGCC AGCTACAATACCCAAGATCATCTGCCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGAAAAACAAGTG TTCTGGAAATGATTGCTCAAGCACGAATATTCCCACGAGGATCCGGTGAGATGATGACGCGCTCTCCAGT GAAGGTGACTCTCAGTGAAGGCCCTCACCACGTGGCCTTGTTTAAAGACAGCTCCCGGGAGTTTGACCTC ACCAAGGAGGAAGATCTTGCAGCGTTAAGACATGAAATTGAACTCCGGATGAGAAAAAATGTGAAAGAAG GTTGTACTGTTAGCCCTGAGACCATATCTTTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTGGT GGACTTGCCTGGTGTTATCAACACTGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGT ATCAGCAAAGCTTACATGCAGAATCCTAACGCCATCATCCTGTGCATTCAAGATGGATCTGTAGATGCCG AGCGCAGTATTGTTACAGACTTGGTCAGTCAAATGGATCCGCATGGAAGAAGAACCATATTTGTTTTGAC CAAAGTAGACCTGGCAGAAAAAAATGTAGCCAGTCCAAGCAGAATACAACAGATAATTGAAGGCAAGCTC TTCCCAATGAAAGCTCTGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGCATTGAAG CTATAAGAGAATATGAAGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACA CCAGGTGACCACGAGAAATCTAAGCCTTGCCGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCGGTT GAACAACAGGCTGATAGTTTTAAAGCGACTCGCTTCAACCTGGAGACAGAGTGGAAGAACAACTACCCCC GCCTGCGGGAGCTCGACAGGAATGAACTATTTGAAAAGGCTAAAAATGAGATCCTCGATGAAGTCATCAG TCTGAGCCAGGTTACTCCAAAGCACTGGGAGGAGATCCTTCAGCAGTCCCTGTGGGAACGAGTGTCAACG CATGTGATCGAGAACATCTACCTCCCAGCTGCCCAGACCATGAACTCAGGAACATTCAACACCACAGTGG ACATCAAGCTTAAACAGTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCTTGGGAGACCCT ACAAGACGAATTTTCCCGCTTCATGACAGAACCCAAAGGAAAGGAACACGATGACATATTTGACAAACTT AAGGAGGCTGTGAAGGAGGAGAGTATCAAGCGGCACAAATGGAACGACTTCGCAGAAGACAGCTTGAGGG TCATCCAGCACAACGCTCTGGAGGACCGGTCCATATCAGATAAGCAACAGTGGGACGCAGCCATCTACTT CATGGAAGAGGCACTTCAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATCGGGCCAGAC TGGAAAAAGAGGTGGATATACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAATGAAACCAAGAATG AATTGGAAAAGATGCTGAAGGTTAATGATGAACACCCAGCTTATCTTGCAAGTGATGAGATTACCACAGT CCGGAAGAACCTGGAATCTCGAGGAGTCGAAGTCGATCCAAGCCTGATTAAGGATACTTGGCATCAAGTC TATAGAAGACATTTCCTAAAAACAGCTCTAAATCACTGTAACCTTTGTCGGAGAGGTTTTTATTACTACC AGAGGCATTTTATAGACTCTGAGCTGGAGTGCAACGACGTGGTCCTGTTCTGGCGAATACAGCGCATGCT CGCTATCACTGCCAACACGCTGAGGCAGCAGCTGACAAACACCGAAGTTAGGCGGCTAGAGAAAAACGTT AAAGAGGTATTAGAAGATTTTGCTGAAGACGGCGAGAAGAAGGTTAAATTGCTTACTGGCAAACGAGTTC AGCTGGCAGAAGATCTCAAGAAAGTTAGAGAAATTCAAGAAAAACTTGATGCTTTCATTGAAGCTCTTCA CCAGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205659 representing NM_133585
Red=Cloning site Green=Tags(s) MWRAGRAALACEVCQSLVKHSSGVQRNVPLQKLHLVSRSIYRSHHPALKLQRPQLRTSFQQFSSLTNLSL HKLKLSPTKYGYQPRRNFWPARLAARLLKLRYIILGSAVGGGYTAKKTFDEWKDMIPDLSDYKWIVPDFI WEIDEYIDLEKIRKALPSSEDLANFAPDLDKIAESLSLLKDFFTAGTPGETAFRATDHGSESDKHYRKVS DKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDA SYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVALFKDSSREFDL TKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGVINTVTSGMAPDTKETIFS ISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPSRIQQIIEGKL FPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTRNLSLAVSDCFWKMVRESV EQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVTPKHWEEILQQSLWERVST HVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQDEFSRFMTEPKGKEHDDIFDKL KEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEALQGRLKDTENAIENMIGPD WKKRWIYWKNRTQEQCVHNETKNELEKMLKVNDEHPAYLASDEITTVRKNLESRGVEVDPSLIKDTWHQV YRRHFLKTALNHCNLCRRGFYYYQRHFIDSELECNDVVLFWRIQRMLAITANTLRQQLTNTEVRRLEKNV KEVLEDFAEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133585 |
| ORF Size | 2880 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133585.3, NP_598269.3 |
| RefSeq Size | 3200 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 171116 |
| UniProt ID | Q2TA68 |
| MW | 111.3 kDa |
| Gene Summary | Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:16778770). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (By similarity). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (By similarity). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205659 | Opa1 (untagged ORF) - Rat optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 12,350.00 |
|
| RR205659L3 | Lenti ORF clone of Opa1 (Myc-DDK-tagged ORF) - Rat optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 14,060.00 |
|
| RR205659L4 | Lenti ORF clone of Opa1 (mGFP-tagged ORF) - Rat optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 15,490.00 |
