Zfyve9 (NM_001107952) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206647 representing NM_001107952 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTATTAGATGAATTTGAACAAAATGAAG ATGAAACAGTTTCTCCTACTTTATTGGATACAAAGTGGAATAAGATTTTAGATTCCTCTTCTCATCCATT GTCTTTTAACCCTGCATTAGCCAATGTGAATGAACCTACAATTTCTAATGAGACAGGACCACAGCGGAAA ATCTTTTCTCTTGCTGGCTCAGCTCCCCTTACAAGGGAGGGAAAAGGTCCCTGTGCTAACGGACAGGACG GTAGTCTAAATCCAGAGATTGACACGATGTGGATTGATGAAAATGCTGTGGCAGAAGACCAACTAATTAA GAGAAACTACAATCAGGATGATCAATTTAGTGCCGTTGAAGTGGGAGAGGAGAAACGTGGGAGCCTCACT TGTCTGCCAGACGAGAAGAATGTTCTGGTTGTAGCTGTCATGCATAACTGTGATAAAAGGACACTGCAAA GTGATTTGCAGGATTGTAATAACTATAATAGTCAATCCCTCATGGATTCTTTTAGCTGTTCACTGGATAA TGAAACTAGACAAACTGATCAATTTAGTTTTAGTATGAATGGGTCCGCAGAAAAAGGTATAAACTCAGAG AAACAAATGGATGCTTTGAATAAACCAAAATCAGAGAGGGATTCTCTTAATCATCTCTGCGCTGCTTCAG CTAACAGTGCAACCAGTATCTCTTCCTCTTCACAATTAAAGGACAATGAAAATGTAGGTAGAGACCCATC TACATCTACAGCTACAAGCTTAGCAATTAATACATCCCAGGGAATGGATGGAGGGCCTGCCATTAAAAAA CAAGAGGATTATATGCCTGATGAGGACCTTTCTGGCGCAAACAGCTCTTCTAGGACAGATCTAGGGAGCT CAAACTCCTTTTCCCACTCAAGGGAGGAGCTCTTAACAAAAACAGAGCCAGCAGAGGAGAGGACAGCTGA AGACCCCCTCCCATCTGGTTTATCTTTGAATCTCAAGCCAGATACTCCTGCTTTATGTGGAAGGGACAAT TGCGAACCATCCTCAGACTGCCTTGTGTCTAGTGAGACTAGAACTGATGGAGATGGAGGTAAGGACTCTC AGACGACTCACTGGGACTCAGCAAAGCTAAATGAGGTGGATGATCGCCAAGGAAGTGAAGAAAATCAAGT GGTTTTACAGATCAGCCAACCTGAGGACCCTCAGAGTGCAGGAGAGTGTGTTAGAATGGCAGATTCAGAT CTAGATTTCAAAGGAACTTGCTTGAATGAAAGTGAAGGATATGATTTCTCCACTGTTAGTGATGCACCAG CAGCAAATTCTCTGTCTAATAGTTGTGATTCCTATGGAATGCAGAGCCCAATTGTATCTTTTGTTCCAAA GACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAAGAGAGCAAATCAGAATGCTAC TCAAATCTTTATGAACAGAGAGGAAATGAGAACACAGAAGGGAATGGACTGCTTTTAAACAGCACTGGAA CTATAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAATCCCATCTGTACTCGGGCAATCTTCCCC CAAGATAGCAAACCTGCAGTCTATTAGTGTTCCTTTTGGTGGTGCAAGACCCAAGCAACCTTCTAATCTT AAACTTCAGATTCCAAAGCCATTATCAGACCATTTACAAAATGACCTTCCTGCAAACAATGGAAATAATA GTAAAAACAAAAATGATATTCTTGGGAAAGCAAAACTAGGGGAAAACTCAGCAGTCAGTGCGTGCAGTGC AACTTTGGGAAACATCTCTGTGACTGATACAAATGGGGAACATTTAGAAAGTTTCGAGGCTGACATCTCC AGTAGACCATGCCTTGCATTATCTCCAGATAGTCCAGATAATGATCTCAGAGCTGGTCAATTTGGAATTT CTGCAAGAAAGCCATTTACCACCCTGGGTGAGGTGGCACCAGTATGGGTACCAGACTCCCAGGCTCCGAA CTGCATGAAGTGTGAAGCTCGGTTTACATTCACCAAAAGGAGGCATCATTGCAGAGCATGTGGGAAGGTT TTCTGTGCTTCCTGCTGCAGCCTGAAATGCAAACTGCTGTACATGGATAGAAAGGAAGCTCGAGTGTGTG TCATCTGTCACTCAGTGCTAATGAACGCTCAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCC TAACCCTAACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCC CTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAAAGCACCCTGGAACAGAAGTGCCTC AGCCCAGAGAGCAGAGGAGAGTTTGGTTTGCTGATGGGATTTTACCCAATGGAGAAGTTGCTGATGCAGC CAAATTAACAATGAATGGAACTTCCTCTGCAGGAACTCTGGCTGTGTCACATGACCCAGTCAAGCCTGTA GCCACCAGTCCTCTACCGGCAGAGGCTGACGTTTCTCTGTTCTCTGGGAGCATTACTCAGGTTGGAAGTC CTGTTGGAAGTGCAATGAACCTTATTCCTGAAGATGGCCTTCCTCCCATCCTCATCTCCACGGGTGTGAA AGGAGATTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTCATGCAGCAACTGGAGGATGGTGGCCCT GACCCACTTGTGTTTGTTTTAAATGCAAATTTATTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGA AGTGCTGGTGTTTTACTACCAAAGGAATGCATGCTGTGGGTCAGTCTGAGATAGTCATTTTGCTACAGTG TCTACCAGATGAGAAATGTTTGCCCAAGGACATCTTTAATCATTTTGTACAACTTTACCGGGATGCACTG GCTGGGAGTGTTGTGGGCAGCCTGGGACATTCTTTCTTCAGTCAGAGCTTCCTGGGCAGTAAAGAGCACG GCGGGTTCCTGTACATGACCTCTACCCACCAGTCACTGCAAGACCTGCTGCTTCCAACACCTCCTTATCT GTTTGGGATTCTCATCCAGAAATGGGAGACCCCTTGGGCCAAAGTGTTTCCTATTCGACTCATGTTAAGA CTTGGAGCTGAGTATCGACTTTACCCCTGTCCACTGTTCAGTGTCAGATTTCGGAAGCCATTGTTCGGAG AAACTGGGCATACCATCATGAATCTTCTAGCAGACTTCCGAAATTACCAGTACACTTTGCCAGGTGTCCA AGGTTTGGTGGTTGATATGGAAGTTCGGAAAACAAGCATCAAAATTCCCAGCAACAGATACAATGAGATG ATGAAAGCCATGAACAAATCCAATGAGCACGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGGCAGATT CTCATCTTGTGTGTGTACAGAACGATGATGGGAACTATCAGACCCAGGCCATCAGTATTCACAATCAGCC CAGGAAAGTGACTGGTGCCAGCTTCTTCGTGTTCAGTGGCGCACTGAAGTCCTCTTCAGGGTACCTTGCC AAGTCCAGTATTGTAGAAGATGGCGTTATGGTCCAGATCACTGCAGAGAACATGGATTCCTTGAGGCAGG CACTGCGGGAGATGAAGGACTTTACCATCACTTGTGGGAAGGCAGATGCAGAGGACCCCCAGGAACAGAT CCACATCCAGTGGGTGGACGACGACAAGACAGTTAACAAAGGTGTGGTGAGTCCTATAGATGGGAAGTCC ATGGAATCTATAACAAATGTGAAAATATTCCATGGGTCAGAGTATAAAGCCAATGGAAAAGTCATCAGAT GGACAGAGGTTTTCTTCCTAGAGAACGATGACCACCGTACCTGCCTGAGTGACCCTGCAGATCACAGCAG ACTGACCGAGCACGTCGCCAAGGCATTCTGCCTGGCCCTCTGCCCCCACCTGAAGCTTTTAAAAGAAGAC GGAATGACAAAACTGGGACTCCGTGTGACGCTTGACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAATG GACAGCCCCTCCCCTCGCAGTACATGAAGGATCTGGACAGTGCCTTGGTGCCTGTCATCCATGGAGGAGC CTGCCAGCTCAGCGAGGGCCCGGTCGGCATCGAGCTCATCTTTTACATCCTGGAGAACATCGCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206647 representing NM_001107952 Red=Cloning site Green=Tags(s) MENYFQAEAYNLDKVLDEFEQNEDETVSPTLLDTKWNKILDSSSHPLSFNPALANVNEPTISNETGPQRK IFSLAGSAPLTREGKGPCANGQDGSLNPEIDTMWIDENAVAEDQLIKRNYNQDDQFSAVEVGEEKRGSLT CLPDEKNVLVVAVMHNCDKRTLQSDLQDCNNYNSQSLMDSFSCSLDNETRQTDQFSFSMNGSAEKGINSE KQMDALNKPKSERDSLNHLCAASANSATSISSSSQLKDNENVGRDPSTSTATSLAINTSQGMDGGPAIKK QEDYMPDEDLSGANSSSRTDLGSSNSFSHSREELLTKTEPAEERTAEDPLPSGLSLNLKPDTPALCGRDN CEPSSDCLVSSETRTDGDGGKDSQTTHWDSAKLNEVDDRQGSEENQVVLQISQPEDPQSAGECVRMADSD LDFKGTCLNESEGYDFSTVSDAPAANSLSNSCDSYGMQSPIVSFVPKTLPSKEDSVTEEKEIEESKSECY SNLYEQRGNENTEGNGLLLNSTGTIMKKNYLHNFCSQIPSVLGQSSPKIANLQSISVPFGGARPKQPSNL KLQIPKPLSDHLQNDLPANNGNNSKNKNDILGKAKLGENSAVSACSATLGNISVTDTNGEHLESFEADIS SRPCLALSPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHHCRACGKV FCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPAEYCSTIPPLQQAQASGA LSSPPPTVMVPVGVLKHPGTEVPQPREQRRVWFADGILPNGEVADAAKLTMNGTSSAGTLAVSHDPVKPV ATSPLPAEADVSLFSGSITQVGSPVGSAMNLIPEDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGP DPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDAL AGSVVGSLGHSFFSQSFLGSKEHGGFLYMTSTHQSLQDLLLPTPPYLFGILIQKWETPWAKVFPIRLMLR LGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPGVQGLVVDMEVRKTSIKIPSNRYNEM MKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASFFVFSGALKSSSGYLA KSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEDPQEQIHIQWVDDDKTVNKGVVSPIDGKS MESITNVKIFHGSEYKANGKVIRWTEVFFLENDDHRTCLSDPADHSRLTEHVAKAFCLALCPHLKLLKED GMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMKDLDSALVPVIHGGACQLSEGPVGIELIFYILENIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107952
ORF Size	4194 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107952.1, NP_001101422.1
RefSeq Size	6457 bp
RefSeq ORF	4197 bp
Locus ID	313477
MW	152.4 kDa

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