Zfyve9 (NM_001107952) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206647 representing NM_001107952 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATTACTTCCAAGCAGAAGCTTACAACCTGGACAAGGTATTAGATGAATTTGAACAAAATGAAG ATGAAACAGTTTCTCCTACTTTATTGGATACAAAGTGGAATAAGATTTTAGATTCCTCTTCTCATCCATT GTCTTTTAACCCTGCATTAGCCAATGTGAATGAACCTACAATTTCTAATGAGACAGGACCACAGCGGAAA ATCTTTTCTCTTGCTGGCTCAGCTCCCCTTACAAGGGAGGGAAAAGGTCCCTGTGCTAACGGACAGGACG GTAGTCTAAATCCAGAGATTGACACGATGTGGATTGATGAAAATGCTGTGGCAGAAGACCAACTAATTAA GAGAAACTACAATCAGGATGATCAATTTAGTGCCGTTGAAGTGGGAGAGGAGAAACGTGGGAGCCTCACT TGTCTGCCAGACGAGAAGAATGTTCTGGTTGTAGCTGTCATGCATAACTGTGATAAAAGGACACTGCAAA GTGATTTGCAGGATTGTAATAACTATAATAGTCAATCCCTCATGGATTCTTTTAGCTGTTCACTGGATAA TGAAACTAGACAAACTGATCAATTTAGTTTTAGTATGAATGGGTCCGCAGAAAAAGGTATAAACTCAGAG AAACAAATGGATGCTTTGAATAAACCAAAATCAGAGAGGGATTCTCTTAATCATCTCTGCGCTGCTTCAG CTAACAGTGCAACCAGTATCTCTTCCTCTTCACAATTAAAGGACAATGAAAATGTAGGTAGAGACCCATC TACATCTACAGCTACAAGCTTAGCAATTAATACATCCCAGGGAATGGATGGAGGGCCTGCCATTAAAAAA CAAGAGGATTATATGCCTGATGAGGACCTTTCTGGCGCAAACAGCTCTTCTAGGACAGATCTAGGGAGCT CAAACTCCTTTTCCCACTCAAGGGAGGAGCTCTTAACAAAAACAGAGCCAGCAGAGGAGAGGACAGCTGA AGACCCCCTCCCATCTGGTTTATCTTTGAATCTCAAGCCAGATACTCCTGCTTTATGTGGAAGGGACAAT TGCGAACCATCCTCAGACTGCCTTGTGTCTAGTGAGACTAGAACTGATGGAGATGGAGGTAAGGACTCTC AGACGACTCACTGGGACTCAGCAAAGCTAAATGAGGTGGATGATCGCCAAGGAAGTGAAGAAAATCAAGT GGTTTTACAGATCAGCCAACCTGAGGACCCTCAGAGTGCAGGAGAGTGTGTTAGAATGGCAGATTCAGAT CTAGATTTCAAAGGAACTTGCTTGAATGAAAGTGAAGGATATGATTTCTCCACTGTTAGTGATGCACCAG CAGCAAATTCTCTGTCTAATAGTTGTGATTCCTATGGAATGCAGAGCCCAATTGTATCTTTTGTTCCAAA GACTTTACCCTCCAAAGAAGATTCAGTAACAGAAGAAAAAGAAATAGAAGAGAGCAAATCAGAATGCTAC TCAAATCTTTATGAACAGAGAGGAAATGAGAACACAGAAGGGAATGGACTGCTTTTAAACAGCACTGGAA CTATAATGAAGAAAAATTATTTACATAATTTCTGTAGTCAAATCCCATCTGTACTCGGGCAATCTTCCCC CAAGATAGCAAACCTGCAGTCTATTAGTGTTCCTTTTGGTGGTGCAAGACCCAAGCAACCTTCTAATCTT AAACTTCAGATTCCAAAGCCATTATCAGACCATTTACAAAATGACCTTCCTGCAAACAATGGAAATAATA GTAAAAACAAAAATGATATTCTTGGGAAAGCAAAACTAGGGGAAAACTCAGCAGTCAGTGCGTGCAGTGC AACTTTGGGAAACATCTCTGTGACTGATACAAATGGGGAACATTTAGAAAGTTTCGAGGCTGACATCTCC AGTAGACCATGCCTTGCATTATCTCCAGATAGTCCAGATAATGATCTCAGAGCTGGTCAATTTGGAATTT CTGCAAGAAAGCCATTTACCACCCTGGGTGAGGTGGCACCAGTATGGGTACCAGACTCCCAGGCTCCGAA CTGCATGAAGTGTGAAGCTCGGTTTACATTCACCAAAAGGAGGCATCATTGCAGAGCATGTGGGAAGGTT TTCTGTGCTTCCTGCTGCAGCCTGAAATGCAAACTGCTGTACATGGATAGAAAGGAAGCTCGAGTGTGTG TCATCTGTCACTCAGTGCTAATGAACGCTCAAGCCTGGGAGAACATGATGAGTGCCTCAAGCCAGAGCCC TAACCCTAACAATCCTGCTGAATACTGTTCTACTATCCCTCCCTTGCAGCAAGCTCAGGCCTCAGGAGCC CTGAGCTCTCCACCTCCCACTGTGATGGTACCTGTGGGAGTTTTAAAGCACCCTGGAACAGAAGTGCCTC AGCCCAGAGAGCAGAGGAGAGTTTGGTTTGCTGATGGGATTTTACCCAATGGAGAAGTTGCTGATGCAGC CAAATTAACAATGAATGGAACTTCCTCTGCAGGAACTCTGGCTGTGTCACATGACCCAGTCAAGCCTGTA GCCACCAGTCCTCTACCGGCAGAGGCTGACGTTTCTCTGTTCTCTGGGAGCATTACTCAGGTTGGAAGTC CTGTTGGAAGTGCAATGAACCTTATTCCTGAAGATGGCCTTCCTCCCATCCTCATCTCCACGGGTGTGAA AGGAGATTATGCTGTGGAAGAGAAACCATCACAGATTTCAGTCATGCAGCAACTGGAGGATGGTGGCCCT GACCCACTTGTGTTTGTTTTAAATGCAAATTTATTGTCAATGGTTAAAATTGTAAATTATGTGAACAGGA AGTGCTGGTGTTTTACTACCAAAGGAATGCATGCTGTGGGTCAGTCTGAGATAGTCATTTTGCTACAGTG TCTACCAGATGAGAAATGTTTGCCCAAGGACATCTTTAATCATTTTGTACAACTTTACCGGGATGCACTG GCTGGGAGTGTTGTGGGCAGCCTGGGACATTCTTTCTTCAGTCAGAGCTTCCTGGGCAGTAAAGAGCACG GCGGGTTCCTGTACATGACCTCTACCCACCAGTCACTGCAAGACCTGCTGCTTCCAACACCTCCTTATCT GTTTGGGATTCTCATCCAGAAATGGGAGACCCCTTGGGCCAAAGTGTTTCCTATTCGACTCATGTTAAGA CTTGGAGCTGAGTATCGACTTTACCCCTGTCCACTGTTCAGTGTCAGATTTCGGAAGCCATTGTTCGGAG AAACTGGGCATACCATCATGAATCTTCTAGCAGACTTCCGAAATTACCAGTACACTTTGCCAGGTGTCCA AGGTTTGGTGGTTGATATGGAAGTTCGGAAAACAAGCATCAAAATTCCCAGCAACAGATACAATGAGATG ATGAAAGCCATGAACAAATCCAATGAGCACGTCCTGGCAGGAGGTGCCTGCTTCAATGAAAAGGCAGATT CTCATCTTGTGTGTGTACAGAACGATGATGGGAACTATCAGACCCAGGCCATCAGTATTCACAATCAGCC CAGGAAAGTGACTGGTGCCAGCTTCTTCGTGTTCAGTGGCGCACTGAAGTCCTCTTCAGGGTACCTTGCC AAGTCCAGTATTGTAGAAGATGGCGTTATGGTCCAGATCACTGCAGAGAACATGGATTCCTTGAGGCAGG CACTGCGGGAGATGAAGGACTTTACCATCACTTGTGGGAAGGCAGATGCAGAGGACCCCCAGGAACAGAT CCACATCCAGTGGGTGGACGACGACAAGACAGTTAACAAAGGTGTGGTGAGTCCTATAGATGGGAAGTCC ATGGAATCTATAACAAATGTGAAAATATTCCATGGGTCAGAGTATAAAGCCAATGGAAAAGTCATCAGAT GGACAGAGGTTTTCTTCCTAGAGAACGATGACCACCGTACCTGCCTGAGTGACCCTGCAGATCACAGCAG ACTGACCGAGCACGTCGCCAAGGCATTCTGCCTGGCCCTCTGCCCCCACCTGAAGCTTTTAAAAGAAGAC GGAATGACAAAACTGGGACTCCGTGTGACGCTTGACTCAGATCAGGTTGGCTATCAAGCAGGGAGCAATG GACAGCCCCTCCCCTCGCAGTACATGAAGGATCTGGACAGTGCCTTGGTGCCTGTCATCCATGGAGGAGC CTGCCAGCTCAGCGAGGGCCCGGTCGGCATCGAGCTCATCTTTTACATCCTGGAGAACATCGCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107952
Insert Size	4197 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107952.1, NP_001101422.1
RefSeq Size	6457 bp
RefSeq ORF	4197 bp
Locus ID	313477

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