Pik3c2g (NM_053923) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206800 representing NM_053923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATACAATTGGCAAACAGAGCCAAACCGTGCTGAACCACAGGAAGGTGGACATGATCACCAGCAGT GTCACCATGCAGACCAGCACCTTTCTTCCAGGCAAGTCAGGTTGGGTTTTGATCAGCTTGTGGAAGAGCT CAGTAACAAAACTCCACTGCCTGAGGATGAAAAAGAAGGCACGTGTTTTGTACCAGATACACCAAACTTG GATTCAAAATGGCAATCCATATATGGACCCCACCCAAGGCACTTCAATGAATTCACTTCTCAGAGTCCCC ACTTCTCCCAGCTTCCTTTTGGAAAAGCATCAGCCATTGGTTTTAATCCTGCTGTATTACCTGCACATCA GTTCATTCATGAGGGAGCCTCCTGGAGAAATCCCACAAGAAAATATCATGGTGGTGAGGATCCCAGGTTC AGTGCTCTAACTCCGTCATCCACTGGCTTGGATAAATGTCATCAACAAGGACAATCAGGGACCGAACATT GTAACTATTATGTGGAACCTGAAAACAATGTTCCCCATCATTATTCACCCTACTCAATGGACTCCATACC CGATAGTGAGGAAAAAGGAAGTGGAGATGCGGATCTTGTAGAACCTTCTCTGGTGTTCTCTAAAGACTCC TTTCTACCCAGGGCATCGGAGAACATGTCAGTGGAAAGCACAGAGCCCATTGGTTGCCCCCTTGAAATAG TTGAAGCACCCCAAGGGAGTAACAAGAGCCTCGCCTCCTTTTGCAACAATGTAACAAAAATAAGAGGACT ATATCATGCAAGTGACACTAATTCCAATTCCGGAAAGATCTGGGCCATCACCACAGCCTATCCATCTCGG CTCTTCGCTGACACCCAGTTCAGAGTTAAAATTTCCACTGATAACTCGGCACAACTTCTTCTTCTTAAGC CACCCGCTAATTATCTTGTCAAAGACCTAATTGCCGAAATTCTACTTTTATGTGCAAATGAGCAGCTTTC CCCCAAAGAGTATCTTCTAAGTATATGCGGTTCTGAGGAATTTTTACAGACGGATCACTGTCTAGGGAGC CACAAAATATTTCAGAAAAGTAAATCTGTCATTCAACTCCATCTCCAGAGAAGCAGGGACACTCCAGGAA AATTATCCCGGAAGAGGGATGATGACCGCAGTCGGGTCCATCTGAACCAACTTCTAGAATTTACACATAT TTGGAAAATATCCAGACAATGCCTCTCCACAGTAATGAAAAGCTACAACCTCCATGTCGAGCACCTGTTG AAAACCCAGGAAGATGTGGAGGAGAAACCTCTGTCATCCATGTTTTCCTGTGGCCGACACCCTCCTCAGC CACATGGGAATGACATTATTGAAGATGTTAGAAACATATGCAGTGTTCTGGGGTGTATTGAAACCAAACA AGTTTCAGATGCAGTAAAAGAACTAACTCTAATTCTGCAGAGACCATCACAGAATTTTCATCAGAATTCA GAGACTTCAAAAAAAGGCTTCATAGAGAACGTGACATCGGAACTGTCGAGGTCCCTCCATCAGCTGGTTG ACGTGTACTGCAGTAGCTTTTGTACAGATTTCCGGCCTGCGCGCGCACCTGGAGGCGTCTCCCGCGACCA CGCTGGGCTCCACTCCCACCTGAGCTTCACGGTGTGTTCCCTGCACAATGTTCCAGAAACTTGGGCACAC AGCTACAAAGCATTTTCATTTTCCTGCTGGCTCACATATGCTGGGAAGAAGCTGTGCCAAGTGAAAAGCT GCAGATCCCTGCCAGTCACAAAGTCATTCTCTTTTTCGGTGAACTGGAATGAAATAATCAATTTTCCTCT TGAGATAAAGTCACTTCCAAGAGAATCCATGCTCGTTATAAAGCTGTTTGGGATTGACAGTGCCACCCAC AGCGCAAATCTGCTGGCCTGGACCTGCCTTCCACTATTTCCAAAAGAAAAGTCTCCGCTGGGGTCTAGGC TTCTCAGCATGACACTACAGAGTGAGCCTCCTATAGAAATGATGGCTCCAGGAGTATGGGATGGGAGCCA GCCTACCCCACTGACCCTGCAGATAGATTTTCCAGCTGCCACGTGGGAGTACGTGAAACCTGAGACTGAA GAGAACAGAACTGACCACCAAGAGCCTCCAAGAGAGTGTTTAAAACACATCGCCAGACTCTCCCAAAAGC AGCCTCCCTTGCTACTTTCTGTGGAAAAGAGGAGATATTTGTGGTTTTATCGTTTCTACTGCAACAATGA GAACTCCTCTCTCCCTCTGGTCTTGGGCAGCGCCCCTGGTTGGGATGAAGGGACAGTTTCGGAAATGCAT GCCGTCTTGAGAAGGTGGACATTTTCCCATCCGTTGGAAGCTCTTGGCCTTTTGACTTCCAGGTTTCCAG ACCAAGACATTCGTGAAGTTGCCGTTCAACAGTTAGACAACTTCTTGACCGATGAGCTGCTGGACTGCCT CCCACAGCTAGTTCAGGCTGTCAAGTTTGAGTGGAGTCTCGAAAGTCCCTTGGTGGAACTCCTGCTTCAT CGATCCTTGCAAAGCATCCGAGTGGCTCACCGCCTGTTCTGGCTGCTGCGGGATGCACAAGGTGAAGACT ACTTTAAAAGCTGGTACCAGGAGCTTTTGGCCGCTCTCCAGTTCTGTGCAGGAGAAGCCCTGATCGAAGA GCTTTCCAAAGAGCAGAAACTTGTCAAACTCCTGGGTGATATTGGAGAAAAAGTGAAGTCGGCTGGCGAT GCTCAGAGAAAGGATGTGCTAAAGAAGGAGATTGGCAGTCTAGAAGAATTCTTTAAAGATATAAAGACTT GCCATCTTCCTCTGAACCCGGCCCTGTGCGTAAAAGGAATTGATCGGGATGCATGTTCATATTTCACATC TAATGCCTTGCCATTGAAGATCACTTTCATCAATGCTAATCCAATGGGCAAAAATATCAGTGTTATTTTT AAGGCCGGCGACGATCTTCGGCAGGATATGCTTGTTCTGCAGATTATTCAAGTGATGGACAACGTTTGGC TTCAGGAGGGCCTCGATATGCAAATGATCATTTATGGATGTCTAGCCACAGGAAAGGCTCAAGGATTCAT AGAGATGGTGCCTGATGCTGTAACGCTTGCCAAGATCCATCTGCACTCTGGGCTGATAGGACCCCTGAAA GAAAACACCATCAAGAAGTGGTTCAGTCAGCACAACCACTTAAAGGAAGATTATGAAAAGGCCTTGAGGA ACTTTTTTTACTCTTGTGCTGGCTGGTGTGTGGTGACATTCATCTTGGGAGTCTGTGACCGACATAATGA CAATATCATGCTGACAAAGTCAGGCCACATGTTTCATATTGACTTTGGAAAATTCTTGGGTCACGCACAA ACATTTGGCGGTATAAAAAGGGACCGAGCGCCTTTCATTTTTACTTCAGAGATGGAGTACTTTATTACGG AGGGTGGGAAAAACACACAGCATTTTCAAGACTTCGTGGAACTCTGCTGCAGAGCCTACAACATTGTGAG GAAGCACAGCCAACTGCTCCTGAGCCTTCTAGAAATGATGCTGCATGCCGGGCTTCCTGAGCTGAGGGGG ATTGAAGACCTGAAATACGTACACGACAATCTCCGGCCACAAGACACAGACCTGGAAGCCACAAGTCATT TTACCACGAAGATAAAGCAGAGTCTGGAGTGCTTCCCAGTTAAACTGAATAACCTGATCCACACGCTTGC ACAGATGCCAGCCTTCAGCCTTGCCAGACCTGCCCCTCAGACTCCTCCCCAGGAGTGCTGCGTCCTGAAT AAAACCAGGACAATTCAGAGAGTCACAATTTTAGGGTTCAGCAAGACACACAGCAACCTGTACCTGATCG AGGTGACACGCAGCGACAACAGGAAAAACCTGGCCAAAAAGTCCTTCGAGCAGTTTTACAGACTTCACAG CCAGATTCAGAAGCAGTTCCCCTTGTTGACTCTCCCAGAGTTTCCTCACTGGTGGCATCTACCTTTCACA GACTCGCACCATGAGAGAATCCGAGATCTGAGTCACTACGTGGAACAGGTGCTGCACGGATCTTACGAAG TCGCAAACAGTGATTGTGTACTCAGTTTTTTTCTCTCTGAACATATACAACAGACCCTTGAAGACTCTCC ATTTGTGGACCCAGGTGACCATTCTCCAGACAAGAGCCCCCAGGTGCAGTTGCTGATGACCTATGAGGAC ACAAAGCTCACCATCCTAGTGAAACACTTGAAAAACATCCATCTCCCAGATGGCTCAGCGCCCAGCGCAC ATGTTGAAATTTATCTTCTGCCACATCCCAGTGAAGTTCGCAGGAAGAAAACAAAGTGCGTTCCAAAATG CACTGACCCAACTTACAATGAAATTGTGGTATATGATGACGTCTCAGGACTTCAGGGACATGTTTTAATG CTCATTGTGAAGAGCAAAACTGTATTTGTGGGAGCGGTTAACATTCAGCTCTGCAGTGTTCCCCTCAATG AAGAAAAGTGGTACCCATTAGGGAACAGTATCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206800 representing NM_053923 Red=Cloning site Green=Tags(s) MAYNWQTEPNRAEPQEGGHDHQQCHHADQHLSSRQVRLGFDQLVEELSNKTPLPEDEKEGTCFVPDTPNL DSKWQSIYGPHPRHFNEFTSQSPHFSQLPFGKASAIGFNPAVLPAHQFIHEGASWRNPTRKYHGGEDPRF SALTPSSTGLDKCHQQGQSGTEHCNYYVEPENNVPHHYSPYSMDSIPDSEEKGSGDADLVEPSLVFSKDS FLPRASENMSVESTEPIGCPLEIVEAPQGSNKSLASFCNNVTKIRGLYHASDTNSNSGKIWAITTAYPSR LFADTQFRVKISTDNSAQLLLLKPPANYLVKDLIAEILLLCANEQLSPKEYLLSICGSEEFLQTDHCLGS HKIFQKSKSVIQLHLQRSRDTPGKLSRKRDDDRSRVHLNQLLEFTHIWKISRQCLSTVMKSYNLHVEHLL KTQEDVEEKPLSSMFSCGRHPPQPHGNDIIEDVRNICSVLGCIETKQVSDAVKELTLILQRPSQNFHQNS ETSKKGFIENVTSELSRSLHQLVDVYCSSFCTDFRPARAPGGVSRDHAGLHSHLSFTVCSLHNVPETWAH SYKAFSFSCWLTYAGKKLCQVKSCRSLPVTKSFSFSVNWNEIINFPLEIKSLPRESMLVIKLFGIDSATH SANLLAWTCLPLFPKEKSPLGSRLLSMTLQSEPPIEMMAPGVWDGSQPTPLTLQIDFPAATWEYVKPETE ENRTDHQEPPRECLKHIARLSQKQPPLLLSVEKRRYLWFYRFYCNNENSSLPLVLGSAPGWDEGTVSEMH AVLRRWTFSHPLEALGLLTSRFPDQDIREVAVQQLDNFLTDELLDCLPQLVQAVKFEWSLESPLVELLLH RSLQSIRVAHRLFWLLRDAQGEDYFKSWYQELLAALQFCAGEALIEELSKEQKLVKLLGDIGEKVKSAGD AQRKDVLKKEIGSLEEFFKDIKTCHLPLNPALCVKGIDRDACSYFTSNALPLKITFINANPMGKNISVIF KAGDDLRQDMLVLQIIQVMDNVWLQEGLDMQMIIYGCLATGKAQGFIEMVPDAVTLAKIHLHSGLIGPLK ENTIKKWFSQHNHLKEDYEKALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMFHIDFGKFLGHAQ TFGGIKRDRAPFIFTSEMEYFITEGGKNTQHFQDFVELCCRAYNIVRKHSQLLLSLLEMMLHAGLPELRG IEDLKYVHDNLRPQDTDLEATSHFTTKIKQSLECFPVKLNNLIHTLAQMPAFSLARPAPQTPPQECCVLN KTRTIQRVTILGFSKTHSNLYLIEVTRSDNRKNLAKKSFEQFYRLHSQIQKQFPLLTLPEFPHWWHLPFT DSHHERIRDLSHYVEQVLHGSYEVANSDCVLSFFLSEHIQQTLEDSPFVDPGDHSPDKSPQVQLLMTYED TKLTILVKHLKNIHLPDGSAPSAHVEIYLLPHPSEVRRKKTKCVPKCTDPTYNEIVVYDDVSGLQGHVLM LIVKSKTVFVGAVNIQLCSVPLNEEKWYPLGNSII TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053923
ORF Size	4515 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053923.1, NP_446375.1
RefSeq Size	5990 bp
RefSeq ORF	4518 bp
Locus ID	116720
UniProt ID	O70173
MW	171 kDa
Gene Summary	catalyzes the addition of a phosphate group to the 3' position for phosphotidylinositol and PtdIns 4-P substrates, but not for PtdIns 4,5-P2 [RGD, Feb 2006]

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