Ehmt1 (NM_001108572) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR206985 representing NM_001108572 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCGGATGCTGAGCAGGCAGTTCTGGCCAAGCAAGAGACCAAGCAGGATTGCTGCATGAAAA CTGAACTGCTAAGGGAAGATACACTTATGGCTGCTGATGAAGGTTCCACAGAGAAGCAAGCAGGAGAGAC TCCTATGGCTGCAGATGGAGAAACCAATGGGTCTTGTGAAAAGAGTGGCGATATCAGCCATCCAAATGCA CCCAAACATACTCAGGAGAACACAAGAGCTAGCCCACAGGAAGGCACCAACAGAGTATCTCGGGTGGCGG AAAATGGGGTTTCAGAAAGAGACACAGAAGTGGGGAAGCAAAACCATGTTACAGCTGACGACTTCATGCA GACGTCTGTCATTGGAAGCAATGGATATTTCTTAAATAAACCAGCCCTGCAGGGACAGCCCTTGAGGACT CCCAACACTCTAAATTCCTCGCTTCCTGGTCATGCCGCAAAAACGCTTCCTGGAGGAGCCAGTAAGTGCA GGACTCCAAGTGCACTTCCTCAGACACCAACCACAGCACCCACTGTGCCTGGGGAAGGGAGTGCAGACAC AGAGGACAGAAAGCCTACAGCCTCGGGCACTGATGTCAGGGTTCACAGGGCACGCAAGACCATGCCGAAG TCCATCCTGGGCCTGCACGCAGCCAGTAAAGACCATAGAGAAGTTCAAGACCATAAGGAACCAAAAGAGG ATATCAACAGAAACATTTCTGAATGTGGACGACAGCAGCTTTTACCAACTTTCCCAGCCCTCCACCAGTC GCTACCTCAGAATCAATGCTACATGGCCACCACAAAGTCCCAGACAGCTTGCTTGCCTTTTGTTTTAGCA GCTGCAGTGTCTCGGAAGAAAAAACGAAGAATGGGAACCTATAGCTTAGTTCCCAAGAAAAAGACCAAAG TATTAAAGCAGAGGACGGTGATTGAGATGTTTAAAAGCATCACTCATTCCACTGTGGGTGCCAAGGGTGA GAAAGTCTTAGATGATAGTGCCCTGCATGTAAATGGCGAGAGCTTGGAGATGGACTCCGAGGAGGAAGAC TCTGAGGAGCTGGAGGATGAGGAGGACCGTGGCGCTGAGCAGGCTGCTGCATTTCCCACTGAGGACAGTA GGACTTCTAAAGAGAGCATGTCTGAGACTGATCGTGCTGCAAAGAGTTCTGAATCTAGTATCAAGAAGAA ATTTCTCAAGAGAAGAGGGAAGACTGACAGCCCCTGGATCAAACCTGCTCGGAAAAGGAGGCGGAGAAGC AGAAAGAAGCCAAGCAGCATGCTTGGTTCCGAGGCTTGTAAGTCATCCCCAGGAAGCATGGAGCAGGCAG CTCTGGGAGACAGCGCTGGGTATATGGAAGTTTCTCTGGATTCCCTGGATCTCCGTGTCAGAGGAATTCT GTCTTCCCAGACGGAAAACGAAGGGCTGGCCAATGGTCCGGATGTGCTGGAGACGGATGGCCTCCATGAA GTGCCTCTCTGCAGCTGCCGCATGGAAACCCCCAAGAGCCGTGAGATCAGCACCCTGGCCAACAACCAGT GCATGGCCACTGAGAGCGTGGATCACGAATTGGGCCGGTGTACAAACAGCGTGGTCAAGTATGAGCTGAT GCGCCCTTCCAACAAAGCACCACTCTTGGTGCTGTGTGAAGACCATCGGGGTCGCATGGTGAAGCACCAG TGCTGTCCTGGCTGCGGCTACTTCTGCACGGCGGGTAACTTCATGGAATGTCAGCCTGAGAGCAGCATCT CTCATCGTTTCCATAAGGACTGTGCCTCTCGAGTCAACAATGCCAGCTACTGTCCCCATTGTGGGGAAGA GACTTCCAAGGCCAAAGAGGTGACCATAGCAAAAGCAGACACGACTTCCACAGTGACCCTGGCCCCTGGA CAGGAGAAGAGCTTGGCTGCTGAGGGCAGGGCTGACACGACCACTGGCAGCATTGCTGGAGCCCCAGAGG ATGAAAAATCGCAGAGCACAGTCACTCAGGCACCAGAGTGCTTCGATCCTGCAGGGCCAGCTGGTTTCGT GAGGCCGACCTCTGGCTTTTCCCAGGGCCCAGGAAAGGAAACCTTGGAAAGTGCTCTCATTGCTCTGGAC TCTGAAAAACCGAAGAAACTTCGCTTCCACCCGAAGCAGCTGTACTTCTCCGCTAGGCAGGGTGAGCTGC AGAAGGTGCTTCTCATGCTGGTTGATGGAATTGATCCCAACTTCAAAATGGAGCACCAAAGTAAGCGTTC CCCATTACACGCTGCTGCAGAGGCTGGCCACGTGGACATCTGCCACATGCTGGTTCAGGCGGGTGCCAAT ATTGACACTTGCTCAGAGGACCAGAGGACCCCCCTGATGGAGGCCGCAGAGAACAACCACTTGGACGCAG TGAAGTACCTCATCAAGGCCGGAGCACAGGTGGATCCGAAGGACGCAGAGGGCTCCACGTGTTTGCACTT GGCTGCCAAGAAAGGCCACTATGACGTGGTTCAGTATCTGCTGTCAAATGGACAGATGGATGTCAACTGC CAGGATGATGGTGGTTGGACACCTATGATCTGGGCCACTGAGTACAAGCACGTGGACCTGGTGAAGCTGC TGCTCTCTAAAGGCTCTGACATCAATATCCGGGACAATGAGGAGAACATTTGTCTGCACTGGGCAGCATT TTCAGGCTGTGTGGACATAGCTGAAATCCTCCTGGCTGCCAAGTGTGACCTGCATGCTGTGAATATCCAC GGAGACTCACCCCTGCACATCGCAGCCAGGGAGAATCGCTACGACTGTGTTGTCCTCTTTCTTTCTCGGG ATTCAGATGTTACTTTGAAAAACAAGGAAGGAGAGACTCCCTTGCAGTGTGCAAGCCTCAATTCCCAGGT GTGGAGTGCACTGCAGATGAGCAAGGCACTTCAGGACTCAGCCCCTGACAAGCCCGTTGCTGTTGAGAAG ACGGTGAGCAGAGACATTGCCCGAGGATATGAGCGCATTCCCATTCCCTGTGTCAATGCTGTGGATAGTG AGTTATGTCCCACCAACTACAAGTACGTCTCCCAGAACTGCGTGACGTCCCCAATGAACATCGACAGGAA CATAACTCATTTGCAGTACTGCGTTTGTGTAGACGACTGCTCCTCTAGCACCTGCATGTGTGGCCAGCTG AGCATGCGCTGCTGGTATGACAAGGACGGGCGACTTCTGCCTGAGTTTAACATGGCAGAACCACCCTTGA TCTTCGAGTGCAATCATGCCTGCTCCTGCTGGAGGAACTGCCGCAATCGCGTGGTGCAGAATGGTCTCAG GGCAAGGCTGCAGCTTTATCGGACACAGGACATGGGCTGGGGTGTGCGGTCCCTTCAGGATATCCCGCTG GGCACCTTTGTCTGCGAATACGTGGGGGAGCTGATTTCAGACTCCGAGGCTGATGTTCGGGAAGAGGACT CTTACCTCTTTGATCTTGACAATAAGGATGGAGAGGTGTACTGCATTGATGCTCGGTTCTACGGGAATGT CAGCCGGTTCATAAACCACCACTGCGAGCCCAACCTTGTGCCTGTGCGAGTGTTCATGTCACACCAGGAC CTACGGTTTCCCAGGATTGCCTTCTTCAGCACCCGCCTGATTCAGGCTGGGGAACAGCTGGGGTTTGACT ATGGGGAACGCTTTTGGGACGTCAAGGGCAAGCTCTTCAGTTGCCGGTGTGGGTCTCCCAAGTGTCGGCA CTCAAGTGCAGCCCTGGCCCAGCGGCAAGCCAGTGCAGCCCAGGAGGCTCAGGAGAATGGCCTTCCAGAT ACCAGCTCTGCAGCTGCTGCTGACCCCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR206985 representing NM_001108572 Red=Cloning site Green=Tags(s) MAAADAEQAVLAKQETKQDCCMKTELLREDTLMAADEGSTEKQAGETPMAADGETNGSCEKSGDISHPNA PKHTQENTRASPQEGTNRVSRVAENGVSERDTEVGKQNHVTADDFMQTSVIGSNGYFLNKPALQGQPLRT PNTLNSSLPGHAAKTLPGGASKCRTPSALPQTPTTAPTVPGEGSADTEDRKPTASGTDVRVHRARKTMPK SILGLHAASKDHREVQDHKEPKEDINRNISECGRQQLLPTFPALHQSLPQNQCYMATTKSQTACLPFVLA AAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGAKGEKVLDDSALHVNGESLEMDSEEED SEELEDEEDRGAEQAAAFPTEDSRTSKESMSETDRAAKSSESSIKKKFLKRRGKTDSPWIKPARKRRRRS RKKPSSMLGSEACKSSPGSMEQAALGDSAGYMEVSLDSLDLRVRGILSSQTENEGLANGPDVLETDGLHE VPLCSCRMETPKSREISTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQ CCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEETSKAKEVTIAKADTTSTVTLAPG QEKSLAAEGRADTTTGSIAGAPEDEKSQSTVTQAPECFDPAGPAGFVRPTSGFSQGPGKETLESALIALD SEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQSKRSPLHAAAEAGHVDICHMLVQAGAN IDTCSEDQRTPLMEAAENNHLDAVKYLIKAGAQVDPKDAEGSTCLHLAAKKGHYDVVQYLLSNGQMDVNC QDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIH GDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDKPVAVEK TVSRDIARGYERIPIPCVNAVDSELCPTNYKYVSQNCVTSPMNIDRNITHLQYCVCVDDCSSSTCMCGQL SMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTQDMGWGVRSLQDIPL GTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMSHQD LRFPRIAFFSTRLIQAGEQLGFDYGERFWDVKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQENGLPD TSSAAAADPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108572
ORF Size	3810 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108572.1, NP_001102042.1
RefSeq Size	4932 bp
RefSeq ORF	3813 bp
Locus ID	362078
MW	139.4 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.