Mtmr4 (NM_001105827) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207050 representing NM_001105827 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAGGAGGGCCCCCCCAGCCTGGAGTACATCCAAGCCAAGGATCTGTTCCCCCCCAAGGAACTGG TGAAGGAGGAGGAAAATCTTCAGGTACCCTTCACAGTGCTACAGGGTGAGGGAGTGGAATTCCTGGGCCG GGCAGCTGATGCCCTCATTGCAATCTCCAACTACCGGCTGCATATCAAGTTCAAAGACTCTGTCATCAAC GTGCCCCTCCGTATGATTGACAGTGTGGAAAGCCGTGATATGTTTCAGTTGCACATTGCCTGCAAGGACT CTAAAGTGGTGAGGTGCCACTTCTCCACTTTCAAGCAGTGCCAAGAGTGGCTCTCAAGGCTAAGCCGGGC CACAGCAAGACCTGCCAAGCCCGAGGATCTCTTTGCCTTTGCCTACCATGCCTGGTGCCTGGGGCTGACT GAGGAGGATCAGCATACCCACCTGTGTCAGCCAGGAGAGCACATCCGATGTCGACAGGAGGCCGAGCTCG CAAGGATGGGTTTTGACCTGCAGAATGTCTGGAGAGTCTCGCATATCAACAGCAATTACAAGCTGTGCCC TAGCTACCCCCAGAAGCTGCTGGTTCCTGTGTGGATCACAGATAAAGAGCTGGAGAATGTGGCTTCTTTC CGCTCTTGGAAGCGCATCCCTGTTGTGGTGTACAGACACCTGCGCAACGGGGCTGCCATTGCCCGCTGCA GCCAGCCTGAGATCAGCTGGTGGGGCTGGCGCAATGCTGATGATGAATACCTCGTCACGTCCATTGCCAA AGCCTGCGCCTTAGACCCAGGGACCAGGGCCAGTGGGGGCTCCCTCAGTGCTGGGACTAATGATGCCAGC GAGGCATGTGACACGGATTTCGATTCTTCTCTGACTGCATGCTCTGGGGTAGAGAGCACAGCAGCCCCCC AAAAACTGCTGATCCTGGATGCACGGTCCTACACAGCAGCAGTGGCCAACCGGGCCAAGGGTGGAGGCTG TGAATGTGAAGAATACTATCCGAACTGTGAGGTCGTGTTTATGGGAATGGCCAACATCCATGCCATCCGG AACAGCTTCCAGTACCTCCGGGCTGTGTGTAGCCAGATGCCAGATCCCAGCAACTGGTTGTCGGCTCTGG AGAGTACCAAATGGCTACAGCACTTGTCAGTGATGCTAAAAGCGGCTGTGTTGGTAGCCAATACAGTAGA TCGGGAAGGCCGGCCTGTGCTGGTGCACTGCTCTGATGGCTGGGATCGTACGCCACAGATTGTAGCCCTG GCCAAAATACTACTGGATCCGTATTACAGGACTCTGGAGGGCTTCCAAGTGTTAGTGGAGTCTGATTGGC TGGATTTTGGGCACAAGTTTGGAGATCGCTGTGGCCATCAGGAAAATGCAGAGGACCAAAATGAACAGTG TCCGGTGTTCCTCCAGTGGCTTGATTCTGTACATCAGCTGCTTAAGCAGTTCCCCTGCCTGTTTGAATTC AATGAAGCATTTCTGGTAAAACTGGTGCAGCACACGTACTCCTGCCTCTACGGCACATTCCTGGCCAACA ACCCCTGCGAGCGAGAGAAGCGCAACATCTATAAACGGACATGCTCGGTGTGGGCACTCCTGCGAGCTGG CAACAAGAACTTCCATAACTTCCTCTACACACCTGGGTCAGACATGGTCCTACATCCCGTGTGTCACGTC CGGGCCCTGCACCTCTGGACAGCTGTTTACCTGCCTGCGTCATCGCCGTGCACCCTTGGAGAGGAGAATA TGGATCTTTACCTTTCCCCAGTGGCTCAGAGCCAGGAATTTTCTGGTCGTTCTCTGGACAGGTTACCTAA AACCAGATCCATGGATGATCTTCTTTCTGCCTGTGATACAAGCAGCCCCCTCACTCGTACTTCCAGTGAC CCGAACCTGAATAACCACTGTCAGGAGGTCAGAGTCAGCCTGGAGCCCTGGCACAGCAATCCTGAAGGAG CAGAGGCAGTCATAGAATCTGGAGTCGGAAGCCCACAGCTGACTGTGGGAGACGTGGGCCTTCCTCCTCC TCTGCCCAGTAGCCAGAAAGACTACTTGAGCAATAAACCTTTCAAGGGTCACAAAAGTTGTTCTCTTAGT TATAAGTTGCTTAATACTTCTGTGTCCTGGGAAATGAAGAACACCTCTGAGATCAAAGTCCTGGAAGAAA CTGAAGCACTGGCCCCGGGCCCTCCAGCCCGAGAGGAGCAGAGTAGGACTTCTGATGGCTTAGGGAAGCC ACCTGAACAGTGTCCAGAGAAGGAAGCTACCAGCTCACTCTCCAATGTTTCCAGCAAGTGTGGAGGAGCC TGTGATTTTCCTGGGGAGCCTCCCCAGGATTCCTTTACAGGTACTCCCCAACAGCCCCATCTAGACTCCA TGCAGGCTGTGCCCATCAGGTGTACTCCAGATCACGGTCAGGGCAGCCTTTGCAACCCACCAAGTGCTGC CAGCCAAACTCTTCAAGAGCCAAATACTGACCTCATCAGCCAAGATCCCCCTGGGTCTATGGCAGGTATC TCCTACCAGGAACAGCCTAGTTCTGTGTCGGATCTGATCTATAAGAAGGAAGATGCTGGCAAGAGAGGAA GTAAAAATGGGCAGTTGGTGGAAAATCCTCGCTTTGGGAAAATGCCATTAGAGTTGGCCCGAAAGCCAAT TTCTCAGAGCCAGATCAGTGAGTTCTCATTTTTAGGGTCAAACTGGGACAGCTTCCAAGGGATGATGACG TCATTCCCCAGTGGAGAGACCACTCCTCGGCGGCTGCTTGCTTATGGCTGTTGTAGCAAGAGGCCGAGCA ATAAGCATATCCGAGCTGCAGCGCCCTGCTTTGGGGGCCAGTGGGCTCAGAGAGAAGGGATGAAGTCGCC CGTCTGCTCTAGTCATTCCAATGGACACTGTACAGGCCCAGGAGGAAAGAACAACCGGATGTGGTTTTCC AGTCATCCAAAGCAAGTCTCCAGCACAAAGCCTTCTCTTCTGAGCTGCCCTTCTCCAGTGCCTCCTCTCT ACCTGGATGATGATGGACTCCCCTTTCCCACGGATGTGATCCAACATAGGTTACGGCAGATCGAAGCAGG GTACAAGCAAGAGGTAGAACAGCTACGTCGACAGGTTCGAGAGCTTCAGATGAGGCTAGACATTCGTCAC TGCTGTGCCCCTCCAGCAGAGCCCCCCATGGACTATGAGGATGACTTTACGTGTTTAAAGGAGTCAGATG GCAGTGACACTGAAGACTTTGGCTCTGATCACAGTGAGGACTGCCTTTCAGAAGCAAGCTGGGAACCTGT TGATAAGAAGGAGACGGAGGTGACTCGCTGGGTTCCAGACCATATGGCATCACACTGCTATAACTGTGAC TGTGAATTCTGGTTGGCCAAACGAAGGCATCATTGCAGAAATTGTGGGAACGTATTTTGTGCTGGATGCT GCCACCTTAAGCTGCCCATTCCCGATCAGCAACTCTATGACCCAGTTCTCGTCTGTAACTCATGTTACGA GCACATTCAAGTGTCTCGTGCCAGGGAGCTCATGAGCCAACATCTGAAGAAACCCATTGCTACAGCTTCC AGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207050 representing NM_001105827 Red=Cloning site Green=Tags(s) MGEEGPPSLEYIQAKDLFPPKELVKEEENLQVPFTVLQGEGVEFLGRAADALIAISNYRLHIKFKDSVIN VPLRMIDSVESRDMFQLHIACKDSKVVRCHFSTFKQCQEWLSRLSRATARPAKPEDLFAFAYHAWCLGLT EEDQHTHLCQPGEHIRCRQEAELARMGFDLQNVWRVSHINSNYKLCPSYPQKLLVPVWITDKELENVASF RSWKRIPVVVYRHLRNGAAIARCSQPEISWWGWRNADDEYLVTSIAKACALDPGTRASGGSLSAGTNDAS EACDTDFDSSLTACSGVESTAAPQKLLILDARSYTAAVANRAKGGGCECEEYYPNCEVVFMGMANIHAIR NSFQYLRAVCSQMPDPSNWLSALESTKWLQHLSVMLKAAVLVANTVDREGRPVLVHCSDGWDRTPQIVAL AKILLDPYYRTLEGFQVLVESDWLDFGHKFGDRCGHQENAEDQNEQCPVFLQWLDSVHQLLKQFPCLFEF NEAFLVKLVQHTYSCLYGTFLANNPCEREKRNIYKRTCSVWALLRAGNKNFHNFLYTPGSDMVLHPVCHV RALHLWTAVYLPASSPCTLGEENMDLYLSPVAQSQEFSGRSLDRLPKTRSMDDLLSACDTSSPLTRTSSD PNLNNHCQEVRVSLEPWHSNPEGAEAVIESGVGSPQLTVGDVGLPPPLPSSQKDYLSNKPFKGHKSCSLS YKLLNTSVSWEMKNTSEIKVLEETEALAPGPPAREEQSRTSDGLGKPPEQCPEKEATSSLSNVSSKCGGA CDFPGEPPQDSFTGTPQQPHLDSMQAVPIRCTPDHGQGSLCNPPSAASQTLQEPNTDLISQDPPGSMAGI SYQEQPSSVSDLIYKKEDAGKRGSKNGQLVENPRFGKMPLELARKPISQSQISEFSFLGSNWDSFQGMMT SFPSGETTPRRLLAYGCCSKRPSNKHIRAAAPCFGGQWAQREGMKSPVCSSHSNGHCTGPGGKNNRMWFS SHPKQVSSTKPSLLSCPSPVPPLYLDDDGLPFPTDVIQHRLRQIEAGYKQEVEQLRRQVRELQMRLDIRH CCAPPAEPPMDYEDDFTCLKESDGSDTEDFGSDHSEDCLSEASWEPVDKKETEVTRWVPDHMASHCYNCD CEFWLAKRRHHCRNCGNVFCAGCCHLKLPIPDQQLYDPVLVCNSCYEHIQVSRARELMSQHLKKPIATAS S TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001105827
ORF Size	3573 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001105827.4, NP_001099297.4
RefSeq Size	5684 bp
RefSeq ORF	3576 bp
Locus ID	287607
MW	132.9 kDa

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