Ppfia2 (NM_001108745) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR207506 representing NM_001108745 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGCGAAGTGATGCCCACGATTAATGAGGACACCCCAATGAGCCAACGGGGGTCCCAAAGTAGTG GCTCTGACTCGGACTCCCACTTTGAGCAACTGATGGTCAATATGCTAGATGAAAGGGACCGTTTACTGGA CACACTTCGAGAGACTCAGGAAAGCCTCTCGCTTGCCCAGCAAAGACTCCAGGATGTCATCTACGACCGA GATTCACTCCAGAGACAGCTTAATTCAGCCCTGCCTCAGGACATCGAATCTCTAACAGGAGGGCTGACTG GTTCTAAGGGGGCTGATCCACCGGAGTTTGCTGCACTGACTAAAGAGCTGAATGCTTGCCGTGAGCAACT TCTAGAAAAGGAAGAAGAGATTTCAGAGCTAAAAGCTGAAAGAAACAACACAAGACTGTTGCTGGAACAC TTGGAGTGCCTTGTGTCCCGACATGAAAGGTCACTAAGAATGACAGTGGTCAAGCGGCAAGCACAGTCTC CCTCTGGAGTGTCCAGTGAAGTGGAGGTTCTCAAGGCACTGAAATCTTTGTTTGAGCACCACAAAGCCTT GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAGGAACTAGCT GCAGCTAATCAGGAGATTGTTGCCTTGCGTGAGCAAAATGTTCATATTCAAAGAAAAATGGTATCGAGTG AGGGGTCCACGGAGTCAGAACATCTTGACGGGATGGAACCAGGCCAGAAAGTCCATGAAAAGCGTCTATC CAATGGCTCCATAGATTCCACAGATGACACCAGCCAGATCGTCGAGCTGCAAGAATTGCTTGAAAAGCAA AACTATGAAATGGCCCAAATGAAAGACCGCTTAGCAGCTCTTTCTTCCCGAGTGGGAGAAGTGGAACAAG AAGCCGAGACGGCAAGAAAGGACCTCATTAAAACAGAAGAAATGAACACCAAATATCAAAGGGACATCCG AGAGGCCATGGCTCAGAAGGAAGATATGGAAGAAAGAATCACAACTCTGGAGAAGCGTTACCTGAGTGCC CAGAGAGAATCTACCTCCATTCACGACATGAATGATAAACTAGAAAATGAGTTGGCAAACAAGGAAGCCA TCTTGCGGCAGATGGAAGAGAAAAACAGGCAATTGCAAGAGCGTCTTGAGCTGGCTGAGCAAAAGTTACA GCAGACCATGAGGAAAGCTGAAACTTTACCTGAAGTAGAGGCAGAACTTGCTCAGAGAATTGCAGCCCTA ACAAAGGCTGAAGAGAGACATGGAAATATTGAAGAGCGTATGAGACACCTAGAGGGTCAACTTGAAGAGA AGAATCAAGAACTTCAAAGAGCTAGGCAAAGGGAGAAAATGAATGAAGAGCATAACAAGAGATTATCTGA CACCGTGGACAGGCTTCTGACAGAATCCAATGAGCGCCTGCAGCTGCACCTAAAGGAAAGGATGGCTGCC TTGGAAGAGAAGAATGTTTTGATTCAAGAATCAGAAACCTTCAGAAAAAATCTTGAAGAGTCCTTACATG ATAAGGAAAGACTAGCAGAAGAAATAGAGAAGTTGAGGTCTGAACTTGACCAAATGAAAATGAGAACTGG CTCTCTAATTGAACCAACTATATCGAGAACTCATCTAGACACCTCCGCTGAGTTGCGGTATTCAGTTGGG TCCCTCGTAGACAGCCAGTCTGATTACAGAACCACTAAAGTAATACGAAGACCAAGGAGAGGCCGCATGG GTGTGCGAAGAGATGAGCCAAAGGTGAAATCCCTTGGGGATCATGAATGGAACAGGACTCAGCAAATTGG AGTACTAGGCAGTCACCCATTCGAAAGTGACACTGAAATGTCAGATATTGATGATGACGACAGAGAAACG ATTTTCAGCTCAATGGATCTTCTCTCTCCAAGTGGCCATTCTGATGCCCAGACGCTAGCTATGATGCTTC AAGAACAACTGGATGCAATCAACAAAGAAATAAGGCTAATCCAGGAAGAAAAGGAATCTACAGAATTGCG TGCTGAAGAAATCGAGAATAGGGTGGCTAGTGTAAGCTTGGAAGGTCTGAACTTGGCAAGGGTCCATCCT GGTACCTCCATCACTGCTTCTGTTACAGCTTCATCACTGGCCAGTTCATCTCCCCCAAGTGGACACTCAA CGCCAAAGCTTACACCAAGAAGCCCGGCCAGGGAAATGGACCGTATGGGAGTCATGACCCTGCCAAGTGA TCTAAGGAAACATCGGAGAAAGATTGCAGTGGTGGAAGAAGATGGGCGGGAAGATAAAGCAACAATTAAA TGTGAAACTTCTCCTCCCCCAACACCCAGAGCCATCAGAATGACTCACACCCTACCTTCTTCCTACCACA ATGAGGCCCGGAGCAGTTTATCTGCCTCTCTTGAGCCAGATAGACTTGGGCTTGGCAGTGCCAATAGCAG CCAGGACTCTCTTCACAAAGCCCCGAAGAAGAAAGGAATCAAGTCTTCAATTGGGCGTTTGTTTGGTAAA AAAGAGAAGGCTCGACTTGGGCAGCTTCGAGGCTTCATGGAGACAGAAGCTGCAGCACAGGAATCGCTGG GCTTAGGCAAACTTGGCACTCAAGCTGAAAAGGACAGAAGGCTGAAGAAAAAGCATGAACTTCTTGAAGA AGCTCGTAGAAAAGGATTACCCTTTGCCCAGTGGGACGGACCTACTGTGGTTGCGTGGCTGGAGCTCTGG CTGGGAATGCCTGCTTGGTATGTGGCAGCATGCAGAGCCAACGTGAAAAGTGGTGCCATCATGTCGGCTT TATCAGACACTGAAATCCAAAGGGAGATTGGAATCAGCAACCCTCTGCACCGCTTAAAGCTCAGACTAGC CATCCAGGAGATGGTTTCCCTCACAAGCCCTTCAGCTCCTCCGACATCGCGAACTCCTTCAGGCAATGTC TGGGTGACCCATGAAGAAATGGAAAACCTCGCAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCT GGGCCCAGACCCTGGCTTATGGAGATATGAACCATGAGTGGATCGGGAACGAATGGCTTCCCAGCCTCGG ATTACCGCAATACCGAAGTTACTTTATGGAATGCTTGGTTGATGCAAGAATGTTAGATCACCTCACAAAA AAGGATCTTCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTTTACAGTATGGGATTATGT GCTTGAAAAGATTGAATTATGATAGGAAAGAGCTAGAGAGAAGACGAGAAGCAAGTCAACATGAAATAAA AGATGTTTTAGTGTGGAGCAATGATCGAGTTATTCGCTGGATCCAAGCGATTGGACTTCGGGAATATGCC AATAACATTCTTGAGAGTGGAGTGCATGGCTCACTCATAGCCCTGGATGAGAACTTTGACTACAGCAGCC TAGCTCTACTGCTGCAGATTCCCACGCAGAACACCCAGGCAAGGCAGATTCTTGAACGCGAGTATAATAA CCTCTTGGCTCTGGGAACTGAGCGGCGACTGGATGAAAGTGATGACAAGAATTTCAGACGCGGATCAACC TGGCGAAGGCAGTTCCCTCCTCGCGAGGTACACGGAATCAGCATGATGCCTGGATCCTCAGAAACATTAC CAGCTGGATTCAGGTTGACCACAACATCTGGGCAGTCCAGAAAAATGACGACAGATGTTGCTTCATCAAG ACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR207506 representing NM_001108745 Red=Cloning site Green=Tags(s) MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDR DSLQRQLNSALPQDIESLTGGLTGSKGADPPEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEH LECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELA AANQEIVALREQNVHIQRKMVSSEGSTESEHLDGMEPGQKVHEKRLSNGSIDSTDDTSQIVELQELLEKQ NYEMAQMKDRLAALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSA QRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAAL TKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAA LEEKNVLIQESETFRKNLEESLHDKERLAEEIEKLRSELDQMKMRTGSLIEPTISRTHLDTSAELRYSVG SLVDSQSDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLGSHPFESDTEMSDIDDDDRET IFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHP GTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIK CETSPPPTPRAIRMTHTLPSSYHNEARSSLSASLEPDRLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGK KEKARLGQLRGFMETEAAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELW LGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNV WVTHEEMENLAAPAKTKESEEGSWAQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTK KDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDRKELERRREASQHEIKDVLVWSNDRVIRWIQAIGLREYA NNILESGVHGSLIALDENFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKNFRRGST WRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108745
ORF Size	3753 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108745.1, NP_001102215.1
RefSeq Size	4986 bp
RefSeq ORF	3756 bp
Locus ID	362876
MW	142.6 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.