United States
Germany
Japan
United Kingdom
China
Atg7 (NM_001012097) Rat Tagged ORF Clone
CAT＃: RR207526
- TrueORF®
Atg7 (Myc-DDK-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug)
"NM_001012097" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 8,930.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Apg7l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR207526 representing NM_001012097
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCTGGACTGTCCAAGCTGCAGTTTGCCCCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGC ACGAACTGACCCAGAAGAAGTTGAACGAGTATCGCCTGGACGAGGCACCCAAAGACATCAAGGGCTATTA CTACAATGGTGACTCTGCCGGCCTCCCCACCCGCTTGACATTGGAGTTCAGTGCTTTTGACATGAGCGCC CCCACGCCTGCCCGCTGCTGCCCGGCCATGGGAACCCTGCACAACACCAACACACTTGAGGCTTTTAAGA CTGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCCAATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGC TCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCTCTTGACCTTCGCGGACCTAAAGAAGTACCACTTC TACTACTGGTTTTGCTGCCCTGCCCTCTGTCTTCCTGAGAGCATCCCTCTAATCCGGGGACCTGTGGGCT TGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCCTGGAGCATGCCTACGATGACCTGTGTCGAACTGA AGGCGTCACAGCCCTGCCATACTTCTTATTCAAGTACGATGACGACACTGTGCTGGTCTCCTTGCTCAAA CACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAGTTAACAGTCGGTGTGTACGATCCCTGTAACCTAA CCCAGCACCCTGGATGGCCTTTGAGGAATTTTTTGGTCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCA GTCTGTTGAAGTTCTCTGCTTTCGGGACCGCACCATGCAGGGAGCAAGAGATGTGACACACAGCATCATC TTTGAAGTGAAACTTCCAGAAATGGCGTTTAGCCCAGATTGTCCTAAGGCAGTTGGCTGGGAGAAGAACC AGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCAGCGGATGTATGGACCCCAAAAGGCTGGCTGAGTC ATCCGTGGATCTGAATCTCAAGCTGATGTGCTGGCGGTTGGTCCCCACCTTGGACTTGGACAAGGTCGTG TCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTGGGGTGTAATGTGGCTAGAACACTGATGGGCTGGG GCGTCAGACACGTCACGTTTGTGGACAACGCCAAGATCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTA TGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAAGGCCCTGGCTGCAGCAGAGCGGCTCCAGAAAATA TTTCCCGGAGTGAATGCCAGCGGGTTCAACATGAGCATCCCCATGCCCGGACACCCTGTGAACTTCTCTG ACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGCAGCTGGAGGAACTCATCGATAGCCACGATGTCAT CTTCCTGCTAATGGACACCAGGGAGAGCCGATGGCTTCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTG GTCATCAATGCTGCCTTGGGGTTTGACACCTTTGTTGTCATGAGACATGGCCTGAAGAAACCCAAGCAGC AGGGAGCCGGGGACCTGTGCCCAAGCCATCTTGTAGCACCTGCTGACCTGGGCTCCTCACTTTTTGCCAA CATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAATGATGTGGTGGCTCCAGGAGATTCCACCAGAGAC CGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCAGGGCTGGCCGTGATTGCAGGAGCCCTGGCTGTGG AGCTGATGGTTTCTGTCTTGCAGCATCCTGAGGGGGGCTACGCCATCGCCAGCAGCAGTGATGACCGCAT GAATGAGCCTCCCACCTCGCTGGGGCTTGTACCTCACCAGATCCGGGGCTTTCTGTCACGGTTCGATAAT GTCCTTCCTGTCAGCCTGGCATTTGATAAATGTACAGCCTGTTCATCCAAAGTTCTTGATCAATATGAGC AAGAAGGATTCACCTTCCTGGCCAAGGTGTTTAACTCTTCACATTCCTTCTTAGAAGATTTGACTGGTCT TACACTGCTCCACCAAGAGACCCAAGCTGCTGAGATCTGGGACATGAGTGACGAGGAGACCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR207526 representing NM_001012097
Red=Cloning site Green=Tags(s) MGDPGLSKLQFAPFNSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPTRLTLEFSAFDMSA PTPARCCPAMGTLHNTNTLEAFKTADKKLLLEQSANEIWEAIKSGAALENPMLLNKFLLLTFADLKKYHF YYWFCCPALCLPESIPLIRGPVGLDQRLSPKQIQALEHAYDDLCRTEGVTALPYFLFKYDDDTVLVSLLK HYSDFFQGQRTKLTVGVYDPCNLTQHPGWPLRNFLVLAAHRWSGSFQSVEVLCFRDRTMQGARDVTHSII FEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSGCMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVV SVKCLLLGAGTLGCNVARTLMGWGVRHVTFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAAERLQKI FPGVNASGFNMSIPMPGHPVNFSDVTMEQARRDVEQLEELIDSHDVIFLLMDTRESRWLPTVIAASKRKL VINAALGFDTFVVMRHGLKKPKQQGAGDLCPSHLVAPADLGSSLFANIPGYKLGCYFCNDVVAPGDSTRD RTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDN VLPVSLAFDKCTACSSKVLDQYEQEGFTFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDEETV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012097 |
| ORF Size | 2094 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012097.1, NP_001012097.1 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 312647 |
| UniProt ID | Q641Y5 |
| MW | 77.4 kDa |
| Gene Summary | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation (By similarity). Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN207526 | Atg7 (untagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 8,930.00 |
|
| RR207526L3 | Lenti ORF clone of Atg7 (Myc-DDK-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 10,740.00 |
|
| RR207526L4 | Lenti ORF clone of Atg7 (mGFP-tagged ORF) - Rat ATG7 autophagy related 7 homolog (S. cerevisiae) (Atg7), (10 ug) |
CNY 11,880.00 |
