United States
Germany
Japan
United Kingdom
China
Sike1 (NM_001012182) Rat Tagged ORF Clone
CAT＃: RR208313
- TrueORF®
Sike1 (Myc-DDK-tagged ORF) - Rat suppressor of IKK epsilon (Sike), (10 ug)
"NM_001012182" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | RGD1311316; Sike |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR208313 representing NM_001012182
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTGTACGATCGAGAAGATCTTGACGGACGCCAAGACGCTGCTGGAGCGGCTGCGCGAACACGATG CGGCGGCCGAGTCGCTGGTGGACCAGTCGGCCGCGCTGCACCGGCGGGTGGCTGCGATGCGGGAGGCGGG CGCCGTGTTGCCGGAGCAGTATCAAGAGGATGCATCCGACGTGAAGGACATGTCTAAATATAAACCTCAC ATTCTGCTGTCTCAAGAGAATACTCAGATTAGAGACTTACAGCAGGAAAACAGAGAGCTGTGGGTCTCCT TGGAGGAGCACCAGGATGCATTGGAACTCATCATGAGCAAGTACCGGAAACAAATGTTACAGTTAATGGT TGCTAAACAAGCAGTGGATGCTGAACCAGTTTTGAAAGCTCATCAGTCTCACTCTGCAGAAATTGAAAGT CAGATCGATAGAATCTGTGAAATGGGAGCAGTGATGCGGAGAGCGGTTCAGGTGGACGATAACCAGTTCT GTAAGATTCAGGAGAAACTAGCTCAACTGGAGCTTGAAAATAAGGAACTTCGAGAATTACTGTCCATCAG CAGTGAGTCTCTCCAGGTTGGAAAAGAAAGCTCTGTGGCTCCCGAGGCTCAGACCATCAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR208313 representing NM_001012182
Red=Cloning site Green=Tags(s) MSCTIEKILTDAKTLLERLREHDAAAESLVDQSAALHRRVAAMREAGAVLPEQYQEDASDVKDMSKYKPH ILLSQENTQIRDLQQENRELWVSLEEHQDALELIMSKYRKQMLQLMVAKQAVDAEPVLKAHQSHSAEIES QIDRICEMGAVMRRAVQVDDNQFCKIQEKLAQLELENKELRELLSISSESLQVGKESSVAPEAQTIR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001012182 |
| ORF Size | 621 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001012182.2, NP_001012182.1 |
| RefSeq Size | 2393 bp |
| RefSeq ORF | 624 bp |
| Locus ID | 362007 |
| UniProt ID | Q5FWT9 |
| MW | 23.6 kDa |
| Gene Summary | Physiological suppressor of IKK-epsilon and TBK1 that plays an inhibitory role in virus- and TLR3-triggered IRF3. Inhibits TLR3-mediated activation of interferon-stimulated response elements (ISRE) and the IFN-beta promoter. May act by disrupting the interactions of IKBKE or TBK1 with TICAM1/TRIF, IRF3 and DDX58/RIG-I. Does not inhibit NF-kappa-B activation pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN208313 | Sike1 (untagged ORF) - Rat suppressor of IKK epsilon (Sike), (10 ug) |
CNY 3,990.00 |
|
| RR208313L3 | Lenti ORF clone of Sike1 (Myc-DDK-tagged ORF) - Rat suppressor of IKK epsilon (Sike), (10 ug) |
CNY 6,080.00 |
|
| RR208313L4 | Lenti ORF clone of Sike1 (mGFP-tagged ORF) - Rat suppressor of IKK epsilon (Sike), (10 ug) |
CNY 6,650.00 |
