Scn4a (NM_013178) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	microI; Nav1.4; NCHVS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR209327 representing NM_013178 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGCTCATCTCTGCCCAACCTGGTCCCCCCGGGTCCCCACTGCCTGCGCCCCTTCACCCCAGAGT CCCTGGCAGCCATAGAGCAGCGGGCGGTGGAGGAGGAGGCCCGGCTGCAGCGGAACAAGCAGATGGAGAT TGAGGAGCCTGAGCGGAAGCCACGCAGTGACCTGGAAGCTGGCAAGAACCTCCCACTCATCTATGGGGAC CCCCCACCCGAAGTCATTGGCATCCCCCTGGAGGACCTGGATCCTTACTACAGTGACAAGAAGACCTTCA TTGTGCTCAACAAAGGAAAGGCCATCTTCCGATTCTCTGCCACGCCTGCCCTCTACCTGCTGAGCCCCTT CAGCATCGTCAGGAGGGTGGCTATCAAGGTGCTCATTCACGCGCTGTTCAGCATGTTTATCATGATCACC ATCCTGACCAACTGTGTGTTCATGACCATGAGCAATCCGCCTTCTTGGTCCAAACACGTGGAGTACACCT TCACGGGGATCTATACCTTTGAGTCCCTCATTAAGATGCTGGCCCGAGGCTTTTGCATTGATGACTTCAC ATTCCTCCGAGACCCCTGGAACTGGCTGGACTTCAGTGTCATCACAATGGCGTATGTGACAGAGTTTGTG GACTTGGGCAACATCTCAGCCCTGAGGACCTTCCGTGTGCTGCGGGCCCTGAAGACCATCACGGTTATCC CAGGGCTGAAGACAATTGTGGGAGCCCTGATCCAGTCTGTGAAAAAGCTGTCGGATGTGATGATCCTCAC TGTCTTCTGCCTGAGTGTCTTTGCCCTGGTGGGGCTGCAGCTTTTCATGGGAAACCTGCGTCAGAAGTGC GTGCGTTGGCCCCCGCCCATGAATGACACCAACACCACGTGGTATGGCAATGACACTTGGTACAGCAATG ACACTTGGTACGGCAATGACACTTGGTACATCAATGACACTTGGAACAGCCAGGAGAGCTGGGCCGGCAA CTCTACCTTTGACTGGGAGGCCTACATCAATGACGAAGGGAACTTCTATTTCTTGGAGGGCTCCAATGAT GCTCTGCTCTGTGGGAATAGCAGTGATGCTGGGCACTGCCCTGAGGGCTACGAATGCATAAAGGCTGGGC GGAACCCCAACTATGGCTACACCAGCTATGACACCTTCAGCTGGGCTTTCCTGGCTCTCTTCCGGCTCAT GACGCAGGACTACTGGGAGAACCTTTTCCAGCTGACCCTACGAGCTGCTGGCAAGACCTACATGATCTTC TTCGTGGTCATCATCTTCCTGGGCTCCTTCTACCTCATCAATCTGATCCTGGCCGTGGTGGCCATGGCGT ACGCTGAGCAGAATGAGGCTACCCTGGCCGAAGACCAGGAGAAAGAGGAGGAGTTCCAACAGATGCTTGA GAAATACAAAAAACATCAGGAGGAACTGGAAAAGGCTAAGGCTGCCCAGGCTCTGGAAAGTGGAGAGGAG GCAGATGGGGACCCAACCCACAACAAAGACTGCAATGGGAGCCTGGATGCATCCGGGGAGAAGGGGCCCC CAAGGCCAAGCTGCAGCGCAGACAGTGCCATCTCAGATGCTATGGAGGAGCTGGAAGAGGCCCATCAGAA GTGCCCACCGTGGTGGTACAAGTGTGCACACAAAGTCCTCATCTGGAACTGCTGTGCCCCGTGGGTGAAG TTCAAACATATAATCTACCTGATCGTCATGGACCCCTTTGTGGACCTGGGTATCACCATCTGCATTGTGC TCAACACCCTCTTCATGGCCATGGAGCACTACCCCATGACCGAGCACTTTGACAACGTGCTCTCCGTGGG CAACTTGGTCTTCACAGGCATCTTCACTGCGGAGATGGTGTTGAAGCTGATTGCCATGGACCCCTACGAG TATTTCCAACAGGGCTGGAACATCTTTGACAGTTTCATCGTCACCCTCAGCCTGGTGGAGCTGGGCCTGG CCAACGTACAGGGGCTGTCAGTGCTCCGTTCCTTCCGCCTGCTGCGTGTCTTCAAGCTGGCCAAGTCATG GCCAACACTCAACATGCTCATCAAAATCATTGGCAACTCAGTGGGCGCGCTGGGCAACCTGACCCTGGTG CTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGCATGCAGCTGTTCGGCAAGAGCTATAAGGAGTGTG TGTGCAAGATCGCCTCAGACTGCAACCTGCCTCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTCAT CGTCTTCCGCATCCTCTGCGGGGAATGGATCGAGACCATGTGGGACTGCATGGAGGTGGCCGGCCAGGCC ATGTGCCTCACCGTCTTCCTCATGGTCATGGTCATTGGCAACCTGGTGGTCCTGAATCTGTTCCTGGCTC TCCTGCTGAGTTCCTTCAGTGCTGACAGCCTGGCGGCCTCGGATGAGGATGGCGAGATGAACAACCTACA GATTGCCATCGGGCGTATCAAGTGGGGCATCGGTTTTGCCAAAACCTTCCTCCTGGGGCTGTTGCGTGGC AAGATCCTGAGCCCCAAGGAAATAATACTCAGCCTCGGTGAGCCCGGGGGTGCCGGGGAAAATGCCGAGG AGAGTACTCCCGAGGATGAGAAGAAGGAGCCGCCACCGGAAGATAAGGAGCTGAAAGACAATCATATCCT GAACCACGTGGGCCTGACCGATGGCCCCCGCTCCAGCATCGAGCTGGACCACCTTAACTTCATCAACAAC CCCTACCTCACCATCCAGGTGCCCATTGCCTCCGAGGAGTCTGACCTGGAGATGCCCACAGAGGAGGAGA CAGACGCCTTCTCGGAGCCTGAGGATATCAAGAAGCCCCTACAGCCCCTCTACGACGGGAACTCCTCCGT CTGCAGCACAGCTGACTACAAGCCCCCTGAAGAGGACCCCGAGGAGCAGGCTGAGGAGAACCCCGAGGGG GAGCAGCCTGAGGAATGCTTCACGGAAGCCTGTGTGAAGCGCTGCCCCTGCCTCTATGTGGACATCTCCC AGGGCCGTGGGAAAATGTGGTGGACACTGCGCAGGGCCTGTTTCAAGATTGTTGAGCACAACTGGTTTGA GACCTTCATTGTCTTCATGATCTTGCTCAGCAGTGGAGCCCTGGCCTTCGAGGACATCTACATTGAACAG CGACGAGTCATCCGCACCATCCTGGAATACGCCGACAAGGTCTTCACCTACATCTTCATCCTGGAGATGT TGCTAAAGTGGGTCGCCTACGGCTTCAAGGTGTATTTCACCAATGCCTGGTGCTGGCTCGACTTCCTCAT CGTGGACGTCTCCATCATCAGCCTGGTGGCAAACTGGCTGGGCTACTCTGAGCTGGGGCCCATCAAATCC CTGCGCACACTCCGGGCCCTGCGACCTCTGAGGGCACTGTCTCGATTTGAGGGTATGAGGGTGGTGGTGA ACGCCCTCCTGGGAGCTATCCCCTCCATCATGAACGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTT CAGCATCATGGGGGTCAACTTGTTTGCTGGAAAGTTCTACTACTGCGTCAACACCACCACCTCTGAGAGA TTCGACATCTCGGTGGTCAACAACAAGTCCGAGTCTGAGAGCCTCATGTATACAGGCCAGGTCCGCTGGA TGAATGTCAAGGTCAACTACGACAACGTGGGTCTGGGCTATCTCTCCCTCCTTCAGGTGGCCACATTCAA GGGTTGGATGGATATCATGTATGCAGCTGTGGACTCCCGGGAGAAAGAGGAGCAGCCACACTATGAGGTG AACCTCTACATGTACCTCTACTTCGTCATCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTCTTCA TCGGTGTCATCATCGACAACTTCAACCAACAGAAGAAGAAGTTTGGAGGGAAAGACATCTTCATGACAGA GGAACAGAAGAAATACTACAATGCGATGAAGAAGCTTGGCTCCAAGAAGCCCCAGAAGCCAATTCCCCGG CCTCAGAACAAGATCCAGGGCATGGTGTACGACTTCGTGACGAAGCAGGTGTTTGACATCTCCATCATGA TCCTCATCTGCCTCAACATGGTTACTATGATGGTGGAGACAGATGACCAGAGCCAGCTCAAGGTGGACAT CCTGTACAACATCAACATGGTCTTCATTATCATCTTCACAGGGGAGTGTGTGCTGAAGATGTTTGCCCTA CGCCATTACTACTTCACCATTGGCTGGAACATCTTTGACTTTGTGGTTGTCATCTTGTCCATCGTGGGCC TTGCGCTCTCTGACTTGATACAGAAATACTTTGTGTCACCCACGCTGTTCCGTGTGATCCGCTTGGCTCG GATCGGGCGTGTCCTGCGTCTGATCCGCGGGGCCAAGGGCATCCGGACGCTGCTGTTCGCCCTGATGATG TCCCTGCCCGCCCTCTTCAACATCGGCCTCCTTCTCTTTCTGGTCATGTTCATCTACTCGATCTTCGGCA TGTCTAACTTCGCCTACGTCAAGAAAGAGTCAGGCATCGACGACATGTTTAACTTTGAGACCTTTGGCAA CAGTATCATCTGCCTCTTCGAGATCACCACGTCAGCCGGCTGGGACGGGCTTCTGAACCCCATCCTCAAC AGTGGGCCCCCAGACTGTGACCCGACGTTGGAGAACCCGGGTACCAATGTCAGGGGGGACTGCGGCAACC CTTCCATCGGCATCTGTTTCTTCTGCAGCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACAT TGCTATCATCCTGGAGAATTTCAACGTGGCCACCGAAGAGAGCAGCGAGCCCCTCAGTGAGGATGACTTC GAGATGTTCTATGAGACCTGGGAGAAGTTCGACCCGGACGCCACTCAGTTCATCGACTACAGCCGCCTCT CGGACTTTGTGGACACCCTGCAGGAGCCACTGAAAATCGCCAAACCCAATAAGATCAAGCTTATCACGTT AGACCTGCCCATGGTGCCCGGCGACAAGATCCACTGCTTGGACATCCTCTTTGCCCTGACCAAAGAGGTA CTGGGTGACTCTGGGGAGATGGACGCCCTCAAGCAGACCATGGAGGAGAAGTTTATGGCAGCTAACCCTT CCAAGGTCTCCTATGAGCCCATCACCACCACCCTCAAGAGGAAGCAGGAGGAGGTGTGTGCTATCAAAAT CCAGAGGGCCTACCGCCGCCACCTGCTGCAGCGCTCCGTGAAGCAGGCCTCTTACATGTACCGTCACAGC CAGGACGGCAATGACGACGGGGCCCCCGAGAAGGAGGGATTGCTTGCCAACACCATGAACAAGATGTATG GCCATGAGAAAGAGGGTGATGGTGTGCAGAGCCAGGGGGAGGAAGAGAAGGCCTCTACAGAGGATGCTGG ACCCACCGTGGAGCCCGAACCCACCAGCAGCTCAGATACTGCCCTGACTCCTTCTCCTCCACCACTACCC CCCTCATCATCACCGCCACAAGGGCAGACAGTTCGCCCAGGGGTCAAAGAGTCTCTTGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR209327 representing NM_013178 Red=Cloning site Green=Tags(s) MASSSLPNLVPPGPHCLRPFTPESLAAIEQRAVEEEARLQRNKQMEIEEPERKPRSDLEAGKNLPLIYGD PPPEVIGIPLEDLDPYYSDKKTFIVLNKGKAIFRFSATPALYLLSPFSIVRRVAIKVLIHALFSMFIMIT ILTNCVFMTMSNPPSWSKHVEYTFTGIYTFESLIKMLARGFCIDDFTFLRDPWNWLDFSVITMAYVTEFV DLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKC VRWPPPMNDTNTTWYGNDTWYSNDTWYGNDTWYINDTWNSQESWAGNSTFDWEAYINDEGNFYFLEGSND ALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIF FVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKYKKHQEELEKAKAAQALESGEE ADGDPTHNKDCNGSLDASGEKGPPRPSCSADSAISDAMEELEEAHQKCPPWWYKCAHKVLIWNCCAPWVK FKHIIYLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDNVLSVGNLVFTGIFTAEMVLKLIAMDPYE YFQQGWNIFDSFIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLV LAIIVFIFAVVGMQLFGKSYKECVCKIASDCNLPRWHMNDFFHSFLIVFRILCGEWIETMWDCMEVAGQA MCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIGRIKWGIGFAKTFLLGLLRG KILSPKEIILSLGEPGGAGENAEESTPEDEKKEPPPEDKELKDNHILNHVGLTDGPRSSIELDHLNFINN PYLTIQVPIASEESDLEMPTEEETDAFSEPEDIKKPLQPLYDGNSSVCSTADYKPPEEDPEEQAEENPEG EQPEECFTEACVKRCPCLYVDISQGRGKMWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIYIEQ RRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKS LRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCVNTTTSER FDISVVNNKSESESLMYTGQVRWMNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPHYEV NLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPR PQNKIQGMVYDFVTKQVFDISIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMFAL RHYYFTIGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMM SLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILN SGPPDCDPTLENPGTNVRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLSEDDF EMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLKIAKPNKIKLITLDLPMVPGDKIHCLDILFALTKEV LGDSGEMDALKQTMEEKFMAANPSKVSYEPITTTLKRKQEEVCAIKIQRAYRRHLLQRSVKQASYMYRHS QDGNDDGAPEKEGLLANTMNKMYGHEKEGDGVQSQGEEEKASTEDAGPTVEPEPTSSSDTALTPSPPPLP PSSSPPQGQTVRPGVKESLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_013178
ORF Size	5520 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_013178.1, NP_037310.1
RefSeq Size	6957 bp
RefSeq ORF	5523 bp
Locus ID	25722
UniProt ID	P15390
MW	208.9 kDa
Gene Summary	voltage-gated Na+ channel subunit [RGD, Feb 2006]

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