Ppargc1b (NM_176075) Rat Tagged Lenti ORF Clone
CAT#: RR209465L4
- LentiORF®
Lenti ORF clone of Ppargc1b (mGFP-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma, coactivator 1 beta (Ppargc1b), (10 ug)
"NM_176075" in other vectors (3)
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CNY 15,770.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Synonyms | Perc; PGC1beta |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR209465).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_176075 |
ORF Size | 2970 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176075.2, NP_788264.1 |
RefSeq Size | 3163 bp |
RefSeq ORF | 2973 bp |
Locus ID | 291567 |
UniProt ID | Q811R2 |
Gene Summary | Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcriptional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be part of the pathways regulating the elevation of gluconeogenesis, beta-oxidation of fatty acids and ketogenesis during fasting. Stimulates SREBP-mediated lipogenic gene expression in the liver. Induces energy expenditure and antagonizes obesity when overexpressed. Induces also the expression of mitochondrial genes involved in oxidative metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209465 | Ppargc1b (untagged ORF) - Rat peroxisome proliferator-activated receptor gamma, coactivator 1 beta (Ppargc1b), (10 ug) |
CNY 7,256.00 |
|
RR209465 | Ppargc1b (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma, coactivator 1 beta (Ppargc1b), (10 ug) |
CNY 7,976.00 |
|
RR209465L3 | Lenti ORF clone of Ppargc1b (Myc-DDK-tagged ORF) - Rat peroxisome proliferator-activated receptor gamma, coactivator 1 beta (Ppargc1b), (10 ug) |
CNY 14,350.00 |
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