Hsd17b4 (NM_024392) Rat Tagged ORF Clone
CAT#: RR209960
- TrueORF®
Hsd17b4 (Myc-DDK-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4), (10 ug)
"NM_024392" in other vectors (3)
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CNY 9,500.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MPF-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR209960 representing NM_024392
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCGCCTCTGAGGTTCGACGGGCGTGTGGTCCTGGTCACCGGCGCCGGGGGAGGGTTGGGCAGAG CTTATGCCCTGGCTTTTGCAGAAAGAGGAGCATTAGTTGTTGTGAATGACTTAGGAGGGGACTTCAAAGG CGTTGGGAAAGGCTCTTCTGCCGCAGACAAGGTCGTGGAAGAAATAAGAAGGAGAGGCGGGAAAGCGGTG GCCAATTACGATTCAGTCGAAGCAGGCGAGAAGCTTGTGAAGACAGCACTGGACACATTCGGCAGAATAG ATGTTGTGGTGAACAATGCTGGGATCCTGAGGGACCGTTCCTTCTCTAGGATAAGTGATGAAGACTGGGA TATAATTCAAAGAGTTCATTTGCGGGGCTCCTTCCAAGTGACCCGGGCAGCATGGGATCATATGAAGAAG CAGAATTATGGAAGAATCATTATGACGGCCTCAGCTTCTGGAATATACGGCAACTTTGGCCAGGCAAATT ATAGTGCTGCAAAGCTGGGCCTTCTGGGTCTCGCCAATACTCTCGTGATTGAAGGCAGGAAGAACAACAT TCATTGTAACACCATTGCCCCAAACGCTGGGTCACGGATGACAGAGACGGTGATGCCAGAAGACCTCGTT GAAGCCCTGAAGCCAGAGTATGTGGCACCGCTGGTCCTTTGGCTTTGCCATGAGAGCTGTGAGGAAAATG GTGGCTTGTTTGAGGTTGGAGCAGGATGGATTGGAAAATTGCGCTGGGAGAGGACCCTGGGAGCCATTGT CAGGAAGCGGAATCAGCCCATGACTCCCGAGGCAGTGAGGGACAACTGGGTGAAGATCTGTGACTTCAGC AATGCCAGCAAGCCGAAGAGCATTCAAGAGTCCACAGGTGGTATAATCGAAGTTTTACATAAAATAGATT CAGAAGGAATCTCACAAAATCACACCGGTCAAGTGGCATCTGCAGATGCATCAGGATTTGCTGGCGTCGT TGGCCACAAACTTCCTTCATTTTCTTCTTCATATACGGAACTGCAGTGCATTATGTATGCCCTCGGAGTA GGAGCTTCAGTCAAAAATCCAAAGGACTTGAAGTTTGTTTATGAAGGGAGTGCTGACTTCTCCTGTTTGC CTACATTTGGAGTCATTGTCGCTCAGAAGTCCTTGATGAGTGGAGGCTTAGCAGAGGTTCCTGGGCTGTC AATCAACTTTGCAAAGGTTCTTCATGGGGAGCAGTACTTGGAGTTGTATAAGCCACTTCCCCGATCAGGG GAATTAAAATGTGAAGCAGTTATTGCTGACATCCTGGATAAAGGCTCTGGCATAGTGATTGTTATGGACG TCTATTCTTATTCTGGCAAGGAACTTATATGCTATAATCAGTTCTCTGTCTTCGTTGTTGGCTCTGGAGG CTTTGGTGGAAAACGGACATCAGAAAAACTCAAAGCAGCTGTAGCCGTACCAAGTCGGCCTCCAGATGCT GTACTGAGAGATACCACTTCACTGAATCAGGCCGCTCTGTACCGCCTCAGTGGAGACTCGAATCCTTTAC ACATTGACCCGAGCTTTGCGAGCATTGCCGGTTTTGAGAAACCCATATTACACGGATTATGTACTTTTGG GTTTTCTGCAAGGCATGTTTTACAGCAGTTTGCGGATAATGATGTGTCAAGATTCAAGGCCATTAAGGTT CGTTTTGCCAAACCAGTGTATCCAGGACAAACTCTACAAACTGAGATGTGGAAGGAAGGAAACAGAATTC ATTTTCAAACCAAGGTCCAAGAGACTGGAGACATTGTCATTTCCAATGCATATGTGGATCTTGTTCCTAC ATCTGGAGTTTCCGCTCAGACACCTTCTGAGGGTGGAGCACTGCAGAGTGCTCTTGTATTTGGGGAAATA GGTCGACGCCTCAAGGATGTTGGACGTGAGGTGGTAAAGAAAGTAAATGCTGTATTTGAATGGCATATCA CGAAAAATGGGAATGTTGCAGCCAAGTGGACCATTGACCTGAAGAACGGCTCTGGAGAGGTTTACCAAGG CCCTGCCAAAGGCTCTGCTGACACGACCATCACAATTTCTGATGAGGATTTCATGGAAGTGGTCCTGGGC AAGCTTAACCCACAGAATGCCTTCTTCAGTGGCAGACTGAAGGCCCGAGGAAACATCATGCTGAGCCAGA AGCTACAGATGATTCTGAAAGACTATGCCAAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR209960 representing NM_024392
Red=Cloning site Green=Tags(s) MASPLRFDGRVVLVTGAGGGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSSAADKVVEEIRRRGGKAV ANYDSVEAGEKLVKTALDTFGRIDVVVNNAGILRDRSFSRISDEDWDIIQRVHLRGSFQVTRAAWDHMKK QNYGRIIMTASASGIYGNFGQANYSAAKLGLLGLANTLVIEGRKNNIHCNTIAPNAGSRMTETVMPEDLV EALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRKRNQPMTPEAVRDNWVKICDFS NASKPKSIQESTGGIIEVLHKIDSEGISQNHTGQVASADASGFAGVVGHKLPSFSSSYTELQCIMYALGV GASVKNPKDLKFVYEGSADFSCLPTFGVIVAQKSLMSGGLAEVPGLSINFAKVLHGEQYLELYKPLPRSG ELKCEAVIADILDKGSGIVIVMDVYSYSGKELICYNQFSVFVVGSGGFGGKRTSEKLKAAVAVPSRPPDA VLRDTTSLNQAALYRLSGDSNPLHIDPSFASIAGFEKPILHGLCTFGFSARHVLQQFADNDVSRFKAIKV RFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLVPTSGVSAQTPSEGGALQSALVFGEI GRRLKDVGREVVKKVNAVFEWHITKNGNVAAKWTIDLKNGSGEVYQGPAKGSADTTITISDEDFMEVVLG KLNPQNAFFSGRLKARGNIMLSQKLQMILKDYAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024392 |
ORF Size | 2205 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024392.2, NP_077368.2 |
RefSeq Size | 2602 bp |
RefSeq ORF | 2208 bp |
Locus ID | 79244 |
UniProt ID | P97852 |
MW | 79.4 kDa |
Gene Summary | a 17 beta-hydroxysteroid dehydrogenase type IV involved in cholesterol metabolism [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN209960 | Hsd17b4 (untagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4), (10 ug) |
CNY 9,500.00 |
|
RR209960L3 | Lenti ORF clone of Hsd17b4 (Myc-DDK-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4), (10 ug) |
CNY 11,210.00 |
|
RR209960L4 | Lenti ORF clone of Hsd17b4 (mGFP-tagged ORF) - Rat hydroxysteroid (17-beta) dehydrogenase 4 (Hsd17b4), (10 ug) |
CNY 12,350.00 |
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