Igsf1 (NM_175763) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Inhbp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR210061 representing NM_175763 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTTCGGACCTTCACTCTTTTGCTCCTTTGCATTTGGCTCAATCGGGGTATGACTTCAATGGCAG CAGTGGAGTCTCAACCAGAGCTATGGATAGAGTCCAACTACCCCCAGGCCCCTTGGGAGAACATCACACT TTGGTGCAAAAGCCCCTCTCGGGTATCCAGCAAGTTCCTGCTGCTGAAGGATAACACACAGATGACCTGG ATTCATCCTCCTTACAAGACCTTCCAAGTTTCATTCTTCATAGGTGCCCTTACTGAGTCCAATACAGGTC TTTATCGATGCTGCTACTGGAATGAGAAAGGCTGGTCGAAGCCCAGTAAAATTCTAGAGCTAGAAGCACC AGGCCAACTCCCTAAACCTATCTTCTGGATCCAGGCAGAGACCCCCCCTTTTCCTGGATGCAATGTTAAT ATTCTCTGCCATGGATGGCTTCAGGATTTGGTATTTATGCTGTTTAAAGAGGGATACACAGAGCCCATAG ATTACCAAGTCCCAACTGGGACAATGGCCATCTTCTCCATTGACAACTTGGCACCTGAGAATGAAGGCAT TTACATCTGCCGCACCCATATCCAGATGCTCCCCACTCTGTGGTCAGAGCCCAGCAACCCCCTAAAGTTG GTGGTAGCAGGTCTCTACCCCAAACCAACTCTGACAGCCCATCCTGGGCCCATCCTGGCACCAGGAGAAA GCCTGAGTCTCAGGTGCCAAGGGCCAATATATGGAATGACGTTTGCTTTAATGAGGTTGGAAGACTTGAA GAAACCATATTACTACAAGAAGCCAATAAAAAATGAGGCATATTTCTACTTCCAGGCTCTGAAGACACAG GATACAGGGCACTACCTCTGTTTTTACTATGATGGGTCATACAGGGGCTCACTGCTTAGTGATATCCTGA AAATCTGGGTGACTGACACTTTTCCCAAGACCTGGCTACTTGTTCAGCCCAGCCCTGTGATTCAAATGGG TCAAAACGTGAGCCTGAGATGTGGAGGACTGATGGATGGAGTGGGTCTTGCACTCCATAAGAAGGGAGAA GAAAAGCCCCTTCAGTTTCTGGATGCCACCAGCAACACTGGCAACAATTCATTCTTCCTAAAGAATGTGA CCTACAGGGATGCTGGCATCTATAGCTGCCACTATTATCTTACCTGGAAGACTTCCATTAAGATGGCGAC ATACAACACTGTGGAGCTGATTGTTGTAGCTTGGCCCAGTTCCGTGTTCAAAGTAGGAAAGACCATCACC CTTCAGTGCCGAGTATCTCATCCAGTTCTTGAATTTTCTTTGGAATGGGAAGAAACAACAACATTCCAAA AATTCTCAGTAGACGGAGACTTCATCATCACTAACATTGAAGGGCAAGGCACGGGTACCTACAGTTGCAG CTATCGTATTGAGTCACACGCTAACATCTGGTCACATCGCAGTGAGCCTCTCAAGTTGGTGGGGCCAGCA GTCACAGGCTTCCTCCCCTGGAATTCCATTCTGAATGAAGCTATCAGGGTGTCCTTAACGGTGCAGTTTC TTTCCTTGCTGTTGCTAGTGCTGTGGCTACAATGGAAGTGTCGGAGACTGAGACTCAGAGAAGCCTGGTT GTTGGGAACAGCTCAAGGGGTTGCCATGCTCGTCATACTCATAGCTCTTCTTTGCTGTGGGCTGTGCAAT GGGGCATTGACAGAGGAGATTGAAATCATCATGCCAACCCCTAAACCTGAACTGTGGGCAGAGACGAACT TCCCTCAGGCCCCGTGGAAGAACGTAACCCTCTGGTGCAGAAGCCCTTCTGGCTCAACTAAGGAGTTTGT GCTACTAAAGGACGGGACTGGGTGGATTGCAACTCGCCCGGCCTCAGAGCAGGTCCGGGCTGCCTTCCCC CTTGGCGCCCTGACCCAAAGCCACACCGGGAGTTACCATTGCCATTCATGGGAGGAGATGGCTGTGTCGG AGCCTAGTGAAGCACTTGAACTAGTGGGGACAGACATCCTTCCCAAACCTGTCATTTCGGCTTCCCTTCC AATCCGGGGCCAGGAACTGCAGATCCGATGTAAAGGATGGCTGGAAGGCTTGGGGTTTGCTCTGTATAAG ATGGGAGAGCAGGAACCTGTCCAGCAACTCGGTGCTGTTGGGAGAGAAGCCTTCTTTACAATTCAAAGAA TGGAGGATAAAGAGGAGGGCAATTACAGCTGCCGCACACATACTGAAAAGCAGCCCTTCAAGTGGTCTGA GCCCAGTGCTCCCCTGGAGCTTGTCATAAAAGAACTGTACCCCAAGCCTTTCTTCAAGACATGGGCTAGC CCCGTGGTCACTCCTGGTTCTAGAGTGACTTTCAATTGCTCCACCTCCCGGGAACACATGAGTTTTATTC TTTACAAGGATGGCAATGAAATTGCATCCAGTGACCCAGTCTGGGGAAACCCAGGAACCAGCACAGCACA CTTTCTGATCATTTCAGTGGGCATTGGTGATGGAGGAAACTATAGCTGCCGTTATTATGACTTTTCCATC TGGTCTGAGCCCAGCGACCCCGTGGAGCTTATTGTGACAGAATTCTACCCCAAACCCACTCTCCTGGCAC AGCCAGGCCCTGTGGTGCTTCCTGGGAAGAATGTGACCCTGCGCTGTCAAGGAATTTTCCAGGGCATGAG GTTTGCCCTCTTACAGGAGGGTGCTCATGCTCCCTTACAGTTTCAGAGTGCCTCGGGGACCTCCGTTGAC TTCCTCCTCCACACTGTTGGAGCAGAGGACTTTGGGAACTACAGCTGTGTCTACTATGAGACAACCATGT CCAACAGGGGCTCATATCTTAGTACCCCCCTTATGATCTGGGTGACTGACACATTCCCCAGACCATGGTT GTCTGCTGAGCCTAGTTCTGTGGTCACCATGGGGCAGAATGTTACTCTATGGTGCCAAGGACCAGTCCAT GGAGTTGGGTACATTCTGCACAAAGAAGGAGAAGCCACTTCAATGCAGCTTTGGGATTCCACTAGCAATG AAGGGGCATTCCCTATCATCAATATATCTGGTGCTAGCATAGGACGTTATAGCTGCTGTTACCACCCTGA CTGGATGAGCCCTATCAAGATACAGCCTAGCAACACTCTGGAACTCATAGTCACTGGTTTACTCCCTAAG CCCAGCCTGTTAGTCCAGCCTGGTCCCATGGTAGTCCCCGGAGAAAACATGACTTTTCAATGTCAAGGAG AACTGCCAGATTCGACATTTGTCCTGTTGAAGGAGGGGACACAACAGCCCATAGAACAACAGAGGCCAAG TGGGTACAGAGCTGACTTCTGGATGCCAGTGGTGAGGGATCAAGATTCTGGTGTTTACAGCTGTGTTTAT TATCTGGATTCTGCTCCCCTTGTGGCTTCTAATCACAGCAACTCTCTAGAGATCTGGGTGACCGATAAGC CCCCTAAACCCTCTCTGTCAGCGTGGCCCAGCACTGTGTTCAAACTAGGAAAGGATATCACCCTTCAGTG CCGAGGACCCCTGCCAGGAGTTGAATTCGTGCTAGAACACGATGGAGAAGAGGCCCCCCAACAGTTCTCA GAGGATGGAGATTTTGTTATCGGCAACATGGAAGGGAAAGGCATTGGAAACTACAGTTGCAGCTATCGGC TGCAGGCTTACCCTGACATCTGGTCAGAGCCCAGCGACTCCCTGGAGCTGGTTGGAGCCGCAGGGCCTGT TGCTCAAGAGTGCACTGTGGGTAACATTGTCCGAAGTACCCTGATTGTGGTGGTTGTGGTAGCCTTGGGG ATAGTGCTAGCCATAGAGTGGAAGAAGTGGCCTCGACTTCGGACTAGGGGCTCTGAGACAGATGGGAGAG ACCAGACCATAGTCCTTGAAGAGTGTAACCAAGATGGAGAACCCGGCACTGCCACCAATTCTCCTTCATC TGCTTTGCAGGGTGTCTCAGTGGAACAAACAGTCCCAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR210061 representing NM_175763 Red=Cloning site Green=Tags(s) MMLRTFTLLLLCIWLNRGMTSMAAVESQPELWIESNYPQAPWENITLWCKSPSRVSSKFLLLKDNTQMTW IHPPYKTFQVSFFIGALTESNTGLYRCCYWNEKGWSKPSKILELEAPGQLPKPIFWIQAETPPFPGCNVN ILCHGWLQDLVFMLFKEGYTEPIDYQVPTGTMAIFSIDNLAPENEGIYICRTHIQMLPTLWSEPSNPLKL VVAGLYPKPTLTAHPGPILAPGESLSLRCQGPIYGMTFALMRLEDLKKPYYYKKPIKNEAYFYFQALKTQ DTGHYLCFYYDGSYRGSLLSDILKIWVTDTFPKTWLLVQPSPVIQMGQNVSLRCGGLMDGVGLALHKKGE EKPLQFLDATSNTGNNSFFLKNVTYRDAGIYSCHYYLTWKTSIKMATYNTVELIVVAWPSSVFKVGKTIT LQCRVSHPVLEFSLEWEETTTFQKFSVDGDFIITNIEGQGTGTYSCSYRIESHANIWSHRSEPLKLVGPA VTGFLPWNSILNEAIRVSLTVQFLSLLLLVLWLQWKCRRLRLREAWLLGTAQGVAMLVILIALLCCGLCN GALTEEIEIIMPTPKPELWAETNFPQAPWKNVTLWCRSPSGSTKEFVLLKDGTGWIATRPASEQVRAAFP LGALTQSHTGSYHCHSWEEMAVSEPSEALELVGTDILPKPVISASLPIRGQELQIRCKGWLEGLGFALYK MGEQEPVQQLGAVGREAFFTIQRMEDKEEGNYSCRTHTEKQPFKWSEPSAPLELVIKELYPKPFFKTWAS PVVTPGSRVTFNCSTSREHMSFILYKDGNEIASSDPVWGNPGTSTAHFLIISVGIGDGGNYSCRYYDFSI WSEPSDPVELIVTEFYPKPTLLAQPGPVVLPGKNVTLRCQGIFQGMRFALLQEGAHAPLQFQSASGTSVD FLLHTVGAEDFGNYSCVYYETTMSNRGSYLSTPLMIWVTDTFPRPWLSAEPSSVVTMGQNVTLWCQGPVH GVGYILHKEGEATSMQLWDSTSNEGAFPIINISGASIGRYSCCYHPDWMSPIKIQPSNTLELIVTGLLPK PSLLVQPGPMVVPGENMTFQCQGELPDSTFVLLKEGTQQPIEQQRPSGYRADFWMPVVRDQDSGVYSCVY YLDSAPLVASNHSNSLEIWVTDKPPKPSLSAWPSTVFKLGKDITLQCRGPLPGVEFVLEHDGEEAPQQFS EDGDFVIGNMEGKGIGNYSCSYRLQAYPDIWSEPSDSLELVGAAGPVAQECTVGNIVRSTLIVVVVVALG IVLAIEWKKWPRLRTRGSETDGRDQTIVLEECNQDGEPGTATNSPSSALQGVSVEQTVPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175763
ORF Size	3960 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175763.2, NP_786939.1
RefSeq Size	4407 bp
RefSeq ORF	3963 bp
Locus ID	302822
UniProt ID	Q925N6
MW	147.3 kDa
Gene Summary	a putative inhibin coreceptor [RGD, Feb 2006]

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