Gjc1 (NM_001085381) Rat Tagged ORF Clone

CAT#: RR210107

  • TrueORF®

Gja7 (Myc-DDK-tagged ORF) - Rat gap junction membrane channel protein alpha 7 (Gja7), (10 ug)



  "NM_001085381" in other vectors (3)

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CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Cx45; Gja7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR210107 representing NM_001085381
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTGGAGCTTCCTGACTCGCCTGCTAGAGGAGATACACAACCATTCGACGTTTGTAGGGAAGATCT
GGCTCACTGTGCTGATTGTCTTTCGAATTGTCCTAACTGCGGTAGGAGGAGAGTCCATCTACTATGATGA
GCAAAGCAAATTTGTGTGCAACACAGAGCAGCCGGGCTGTGAGAACGTCTGCTATGATGCCTTTGCCCCG
CTCTCCCACGTGCGCTTCTGGGTATTCCAGATCATCCTGGTTGCTACTCCCTCGGTGATGTACCTGGGAT
ATGCTATTCACAAGATTGCCAAAATGGAGCACGGCGAGGCAGACAAGAAGGCAGCTCGGAGCAAACCCTA
TGCCATGCGTTGGAAGCAGCACCGGGCTCTGGAAGAAACGGAAGAGGACCATGAAGAGGATCCTATGATG
TACCCAGAGATGGAGTTAGAAAGCGAGAAAGAAAATAAAGAGCAGAGCCAACCAAAACCCAAGCATGATG
GCCGCCGACGCATTCGTGAGGATGGGCTCATGAAAATCTATGTGTTGCAGCTGCTAGCCAGGACTGTGTT
TGAGGTGGGCTTTCTCATAGGGCAGTATTTCCTGTATGGCTTCCAAGTACACCCATTTTATGTGTGCAGC
AGACTTCCTTGTCCTCATAAGATAGACTGCTTTATTTCTAGACCCACTGAAAAGACCATCTTCCTTCTGA
TAATGTATGGTGTCACAGGCCTCTGCCTATTGCTTAACATTTGGGAGATGCTTCATCTAGGGTTTGGGAC
CATTCGAGACTCACTAAACAGTAAAAGGAGGGAACTTGATGATCCGGGTGCTTATAATTATCCTTTCACT
TGGAATACACCATCTGCTCCCCCAGGCTATAACATTGCTGTCAAACCAGATCAAATCCAGTACACTGAGT
TGTCCAATGCTAAGATTGCCTACAAGCAGAACAAAGCCAATATCGCCCAGGAGCAGCAGTATGGCAGCCA
CGAGGAGCACCTCCCGGCCGATCTGGAGACCCTGCAGCGGGAGATCAGAATGGCCCAGGAACGCTTGGAT
CTAGCAATCCAGGCCTACCATCACCAGAACAACCCCCATGGTCCTCGGGAAAAGAAGGCCAAAGTGGGGT
CCAAATCTGGGTCCAACAAAAGCAGTATTAGTAGCAAATCAGGAGATGGGAAGACCTCCGTCTGGATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR210107 representing NM_001085381
Red=Cloning site Green=Tags(s)

MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAP
LSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDHEEDPMM
YPEMELESEKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCS
RLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELDDPGAYNYPFT
WNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANIAQEQQYGSHEEHLPADLETLQREIRMAQERLD
LAIQAYHHQNNPHGPREKKAKVGSKSGSNKSSISSKSGDGKTSVWI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001085381
ORF Size 1188 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001085381.1, NP_001078850.1
RefSeq Size 1599 bp
RefSeq ORF 1191 bp
Locus ID 266706
UniProt ID A4GG66
MW 45.7 kDa
Gene Summary component of gap junctions; involved in intercellular communication in arterial smooth muscle [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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